Abstract:

When 6 days old barley (Hordeum vulgare L. cv. `Jian 4} seedlings were treated with different concentrations of NaCl for 3 days, the contents of noncovalently conjugated polyamines (PAs) in leaf thylakoid membrane were not changed under low NaCl concentration treatment, but decreased remarkably with NaCl concen tration increasing. Among three kinds of detected noncovalently conjugated PAs, Spd level was the most abundant, which accounted for 40% approximately. However, the contents of covalently conjugated PAs were gradually decreased with NaCl concentration increasing. Among three kinds of detected covalently conjugated PAs, Put level was predominant, which accounted for about 60%. The incorporation of 14C-Glu into thylakoid membrane protein was decreased strikingly by increasing salinity.

Key words: Thylakoid membrane, Polyamines, Incorporation of labelled glutamine, Salt stress, Barley