Chin J Plan Ecolo ›› 2017, Vol. 41 ›› Issue (7): 729-737.doi: 10.17521/cjpe.2016.0270

• Research Articles • Previous Articles     Next Articles

Diversity of dark septate endophyte in the roots of Ammopiptanthus mongolicus and its companion plants

Qian SUN, Zi-Ke XUE, Lin-Lin XIE, Xue-Li HE*(), Li-Li ZHAO   

  1. College of Life Sciences, Hebei University, Baoding, Hebei 071002, China
  • Received:2016-08-29 Accepted:2017-04-05 Online:2017-08-21 Published:2017-07-10
  • Contact: Xue-Li HE E-mail:xuelh1256@aliyun.com
  • About author:

    KANG Jing-yao(1991-), E-mail: kangjingyao_nj@163.com

Abstract:

Aims Dark septate endophytes (DSE) extensively colonize in plant root tissues in different ecosystems. However, our understanding of the ecological significance of DSE in semiarid and arid lands is limited. The main objective of this study was to compare the community composition and species diversity of DSE in Ammopiptanthus mongolicus and its companion plants in desert habitats. Methods Healthy root samples of Ammopiptanthus mongolicus’s companion plants, Artemisia desertorum, Caragana rosea, Nitraria tangutorum and Caragana korshinskii, were collected separately from four main locations of Ammopiptanthus mongolicus plantation in Dengkou of Nei Mongol, Minqin of Gansu, Yinchuan and Shapotou of Ningxia in July 2013. Important findings A total of 103 strains belonging to seven species (i.e Alternaria, Curvularia, Embellisia, Phialocephala, Phoma, Podospora and Pleosporales) of DSE were isolated from the four types of companion plants by means of morphological identification and molecular identification. Among the seven genera, Alternaria, Embellisia, Curvularia and Podospora are found for the first time in desert ecosystems. Ammopiptanthus mongolicus and its companion plants at the same site had significantly different community composition of DSE. Ammopiptanthus mongolicus and its companion plants at different sites had significantly different DSE diversity index. The colonization and species richness of DSE, especially the species and the quantity of spores were higher in roots of Ammopiptanthus mongolicus than in its companion plants. This study supports the results that DSE can form a better symbiotic relationship with the roots of Ammopiptanthus mongolicus than with its companion plants, and can enhance the ecological adaptability of Ammopiptanthus mongolicus to the extreme desert environment.

Key words: dark septate endophyte, community composition, species diversity, Ammopiptanthus mongolicus, companion plants

Table 1

Sampling site characteristics of companion plants of Ammopiptanthus mongolicus"

样地
Site
伴生植物
Companion plant
经纬度
Latitude and
longitude
海拔
Elevation
(m)
年降水量
Mean annual
precipitation (mm)
平均气温
Mean air
temperature (℃)
土壤类型
Soil type
植被类型
Vegetation type
内蒙古磴口
Dengkou, Nei Mongol
沙蒿
Artemisia desertorum
40.43° N, 106.52° E 1 043 145 7.6 流动沙丘
Moving dune
旱生灌木, 半灌木
Desert shrub, subshrub
甘肃民勤
Minqin, Gansu
红花锦鸡儿
Caragana rosea
38.58° N, 102.93° E 1 350 113 9.6 荒漠沙质
Sandy desert
旱生灌木, 半灌木
Desert shrub, subshrub
宁夏银川
Yinchuan, Ningxia
白刺
Nitraria tangutorum
38.6° N, 106.57° E 1 169 203 8.5 石质为主
Stony mainly
荒漠草地, 荒漠灌木
Desert grassland,
desert shrub
宁夏沙坡头
Shapotou, Ningxia
柠条锦鸡儿
Caragana korshinskii
37.57° N, 104.97° E 1 298 186 9.5 沙土为主
Sand mainly
旱生灌木, 半灌木
Desert shrub, subshrub

Table 2

Isolation rate of dark septate endophyte in the companion plants of Ammopiptanthus mongolicus"

样地
Site
伴生植物
Companion plant
菌株数
Number of strains
分离率
Isolation rate (%)
磴口
Dengkou
沙蒿
Artemisia desertorum
49 12.37
民勤
Minqin
红花锦鸡儿
Caragana rosea
11 3.83
银川
Yinchuan
白刺
Nitraria tangutorum
26 9.11
沙坡头
Shapotou
柠条锦鸡儿
Caragana korshinskii
17 4.59

Fig. 1

The morphological characteristics of sporulating dark septate endophyte. A, E, DK-1. B, F, MQ-4. C, G, YC-2-1. D, H, SPT-1."

Fig. 2

The morphological characteristics of sterile dark septate endophyte. A, F, DK-0-10. B, G, DK-4. C, H, MQ-1. D, I, YC-2-1. E, J, YC-2-3."

Table 3

The results of sequence Blast"

样地 Site 分离菌株编号 Number of strains 参考物种(登录号) Reference taxa (accession number) 序列相似性 Similarity of sequence (%)
磴口 Dengkou DK-1 Podospora sp. (JQ354914.1) 97%
DK-0-10 Alternaria alternate (KF731832.1) 99%
DK-4 Alternaria sp. (KC584231.1) 99%
民勤 Minqin MQ-1 Pleosporales sp. (JN859354.1) 97%
MQ-4 Curvularia sp. (JF742784.1) 99%
银川 Yinchuan YC-1 Alternaria japonica (FJ477839.1) 97%
YC-2-1 Embellisia sp. (JN859366.1) 99%
YC-3 Phialocephala fluminis (AF486124.1) 100%
沙坡头 Shapotou SPT-1 Phoma medicaginis (FJ224118.1) 99%

Fig. 3

Neighbor-Joining tree of dark septate endophyte in the roots of the companion plants of Ammopiptanthus mongolicus. DK-1, DK-0-10, DK-4 represent the sequence numbers of ITS1-5.8S-ITS2 form three kinds of dark septate endophyte in the roots of the companion plants of Ammopiptanthus mongolicus in Dengkou; MQ-1, MQ-4, represent the sequence numbers of ITS1-5.8S-ITS2 form two kinds of dark septate endophyte in the roots of the companion plants of Ammopiptanthus mongolicus in Minqin; YC-1, YC- 2-1, YC-3, represent the sequence numbers of ITS1-5.8S-ITS2 form three kinds of dark septate endophyte in the roots of the companion plants of Yinchuan; SPT-1 represents the sequence numbers of ITS1-5.8S-ITS2 form dark septate endophyte in the roots of the companion plants of Ammopiptanthus mongolicus in Shapotou. Other accession numbers in the figure are homologous sequence of dark septate endophyte in the roots of the companion plants of Ammopiptanthus mongolicus.obtained by Blast."

Table 4

Isolation frequency of dark septate endophyte in Ammopiptanthus mongolicus and its companion plants"

样地 Site 植物 Plant 菌株编号
Number of strains
分类
Classification
菌株数量
Quantity of strains
分离频率
Isolation frequency (%)
磴口
Dengkou
沙冬青
Ammopiptanthus
mongolicus
DK-S Cladosporium sp. 16 5.61
DK-1 Podospora sp. 16 5.61
沙蒿
Artemisia desertorum
DK-0-10 Alternaria alternate 23 8.07
DK-4 Alternaria sp. 10 3.51
总菌株数 Total strains 65 22.74
民勤
Minqin
沙冬青
Ammopiptanthus
mongolicus
0-10MQ1217-2-2 Paraphoma chrysanthemicola 30 10.53
2MQ0-10 Phoma sp. 23 8.07
MQ1125 Pleosporales sp. 12 4.21
0-10MQ1217-3 Cladosporium sphaerospermum 16 5.61
红花锦鸡儿
Caragana rosea
MQ-1 Pleosporales sp. 6 2.11
MQ-4 Curvularia eragrostidis 5 1.75
总菌株数 Total strains 92 32.29
银川
Yichuan
沙冬青
Ammopiptanthus
mongolicus
8YC Exophiala cancerae 8 2.81
4YC Phoma sp. 9 3.16
白刺
Nitraria tangutorum
YC-1 Alternaria japonica 8 2.81
YC-2-1 Embellisia sp. 15 5.26
YC-3 Phialocephala fluminis 3 1.06
总菌株数 Total strains 43 15.08
沙坡头
Shapotou
沙冬青
Ammopiptanthus
mongolicus
2SPT1218 Cladosporium cladosporioides 12 4.21
3ASPT1218 Exophiala sp. 28 9.83
6SPT Phialophora mustea 13 4.56
SPT-1125 Exophiala salmonis 15 5.26
柠条锦鸡儿
Caragana korshinskii
SPT-1 Phoma medicaginis 17 5.96
总菌株数 Total strains 85 29.89
合计 Total 285 100

Table 5

The comparison between dark septate endophyte in roots of Ammopiptanthus mongolicus and that in A. mongolicus’s companion plants from different sampling sites"

深色有隔内
生真菌种类
Species of dark
septate endophyte
沙冬青
Ammopiptanthus mongolicus
沙蒿
Artemisia
desertorum
红花锦鸡儿
Caragana
rosea
白刺
Nitraria
tangutorum
柠条锦鸡儿
Caragana
korshinskii
磴口 Dengkou 民勤 Minqin 银川 Yinchuan 沙坡头 Shapotou 磴口 Dengkou 民勤 Minqin 银川 Yinchuan 沙坡头 Shapotou
Alternaria sp. - - - - + - + -
Embellisia sp. - - - - - - + -
Phialocephala sp. - - - - - - + -
Pleosporales sp. - + - - - + - -
Podospora sp. - - - - + - - -
Curvularia sp. - - - - - + - -
Phoma sp. - + + - - - - +
Paraphoma sp. - + - - - - - -
Exophiala sp. - - + + - - - -
Cladosporium sp. + + - + - - - -
Phialophora sp. - - - + - - - -

Table 6

Species diversity of dark septate endophyte in the roots of Ammopiptanthus mongolicus and its companion plants"

样地 Sampling sites 植物 Plant 多样性指数 Diversity index 优势度 Dominance 均匀度 Uniformity
磴口 Dengkou 沙冬青 Ammopiptanthus mongolicus 0.161 6b 0.996 8a -
沙蒿 Artemisia desertorum 0.482 3a 0.989 1a 0.439 0
民勤 Minqin 沙冬青 Ammopiptanthus mongolicus 0.735 1a 0.977 5a 0.530 3a
红花锦鸡儿 Caragana rosea 0.152 2b 0.999 2a 0.219 6b
银川 Yinchuan 沙冬青 Ammopiptanthus mongolicus 0.209 5b 0.998 2a 0.302 3a
白刺 Nitraria tangutorum 0.303 4a 0.996 3a 0.276 2b
沙坡头 Shapotou 沙冬青 Ammopiptanthus mongolicus 0.657 1a 0.983 7a 0.474 0
柠条锦鸡儿 Caragana korshinskii 0.168 1b 0.996 4a -
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