Diversity of dark septate endophyte in the roots of Ammopiptanthus mongolicus and its companion plants

doi:10.17521/cjpe.2016.0270

Abstract

Abstract:

Aims Dark septate endophytes (DSE) extensively colonize in plant root tissues in different ecosystems. However, our understanding of the ecological significance of DSE in semiarid and arid lands is limited. The main objective of this study was to compare the community composition and species diversity of DSE in Ammopiptanthus mongolicus and its companion plants in desert habitats. Methods Healthy root samples of Ammopiptanthus mongolicus’s companion plants, Artemisia desertorum, Caragana rosea, Nitraria tangutorum and Caragana korshinskii, were collected separately from four main locations of Ammopiptanthus mongolicus plantation in Dengkou of Nei Mongol, Minqin of Gansu, Yinchuan and Shapotou of Ningxia in July 2013. Important findings A total of 103 strains belonging to seven species (i.e Alternaria, Curvularia, Embellisia, Phialocephala, Phoma, Podospora and Pleosporales) of DSE were isolated from the four types of companion plants by means of morphological identification and molecular identification. Among the seven genera, Alternaria, Embellisia, Curvularia and Podospora are found for the first time in desert ecosystems. Ammopiptanthus mongolicus and its companion plants at the same site had significantly different community composition of DSE. Ammopiptanthus mongolicus and its companion plants at different sites had significantly different DSE diversity index. The colonization and species richness of DSE, especially the species and the quantity of spores were higher in roots of Ammopiptanthus mongolicus than in its companion plants. This study supports the results that DSE can form a better symbiotic relationship with the roots of Ammopiptanthus mongolicus than with its companion plants, and can enhance the ecological adaptability of Ammopiptanthus mongolicus to the extreme desert environment.

http://jtp.cnki.net/bilingual/detail/html/ZWSB201707003

Key words: dark septate endophyte, community composition, species diversity, Ammopiptanthus mongolicus, companion plants

Qian SUN, Zi-Ke XUE, Lin-Lin XIE, Xue-Li HE, Li-Li ZHAO. Diversity of dark septate endophyte in the roots of Ammopiptanthus mongolicus and its companion plants[J]. Chin J Plant Ecol, 2017, 41(7): 729-737.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2016.0270

https://www.plant-ecology.com/EN/Y2017/V41/I7/729

Figures/Tables 9

References 34

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样地 Site	伴生植物 Companion plant	经纬度 Latitude and longitude	海拔 Elevation (m)	年降水量 Mean annual precipitation (mm)	平均气温 Mean air temperature (℃)	土壤类型 Soil type	植被类型 Vegetation type
内蒙古磴口 Dengkou, Nei Mongol	沙蒿 Artemisia desertorum	40.43° N, 106.52° E	1 043	145	7.6	流动沙丘 Moving dune	旱生灌木, 半灌木 Desert shrub, subshrub
甘肃民勤 Minqin, Gansu	红花锦鸡儿 Caragana rosea	38.58° N, 102.93° E	1 350	113	9.6	荒漠沙质 Sandy desert	旱生灌木, 半灌木 Desert shrub, subshrub
宁夏银川 Yinchuan, Ningxia	白刺 Nitraria tangutorum	38.6° N, 106.57° E	1 169	203	8.5	石质为主 Stony mainly	荒漠草地, 荒漠灌木 Desert grassland, desert shrub
宁夏沙坡头 Shapotou, Ningxia	柠条锦鸡儿 Caragana korshinskii	37.57° N, 104.97° E	1 298	186	9.5	沙土为主 Sand mainly	旱生灌木, 半灌木 Desert shrub, subshrub

样地 Site	伴生植物 Companion plant	经纬度 Latitude and longitude	海拔 Elevation (m)	年降水量 Mean annual precipitation (mm)	平均气温 Mean air temperature (℃)	土壤类型 Soil type	植被类型 Vegetation type
内蒙古磴口 Dengkou, Nei Mongol	沙蒿 Artemisia desertorum	40.43° N, 106.52° E	1 043	145	7.6	流动沙丘 Moving dune	旱生灌木, 半灌木 Desert shrub, subshrub
甘肃民勤 Minqin, Gansu	红花锦鸡儿 Caragana rosea	38.58° N, 102.93° E	1 350	113	9.6	荒漠沙质 Sandy desert	旱生灌木, 半灌木 Desert shrub, subshrub
宁夏银川 Yinchuan, Ningxia	白刺 Nitraria tangutorum	38.6° N, 106.57° E	1 169	203	8.5	石质为主 Stony mainly	荒漠草地, 荒漠灌木 Desert grassland, desert shrub
宁夏沙坡头 Shapotou, Ningxia	柠条锦鸡儿 Caragana korshinskii	37.57° N, 104.97° E	1 298	186	9.5	沙土为主 Sand mainly	旱生灌木, 半灌木 Desert shrub, subshrub

样地 Site	伴生植物 Companion plant	菌株数 Number of strains	分离率 Isolation rate (%)
磴口 Dengkou	沙蒿 Artemisia desertorum	49	12.37
民勤 Minqin	红花锦鸡儿 Caragana rosea	11	3.83
银川 Yinchuan	白刺 Nitraria tangutorum	26	9.11
沙坡头 Shapotou	柠条锦鸡儿 Caragana korshinskii	17	4.59

样地 Site	伴生植物 Companion plant	菌株数 Number of strains	分离率 Isolation rate (%)
磴口 Dengkou	沙蒿 Artemisia desertorum	49	12.37
民勤 Minqin	红花锦鸡儿 Caragana rosea	11	3.83
银川 Yinchuan	白刺 Nitraria tangutorum	26	9.11
沙坡头 Shapotou	柠条锦鸡儿 Caragana korshinskii	17	4.59

样地 Site	分离菌株编号 Number of strains	参考物种(登录号) Reference taxa (accession number)	序列相似性 Similarity of sequence (%)
磴口 Dengkou	DK-1	Podospora sp. (JQ354914.1)	97%
	DK-0-10	Alternaria alternate (KF731832.1)	99%
	DK-4	Alternaria sp. (KC584231.1)	99%
民勤 Minqin	MQ-1	Pleosporales sp. (JN859354.1)	97%
民勤 Minqin	MQ-4	Curvularia sp. (JF742784.1)	99%
银川 Yinchuan	YC-1	Alternaria japonica (FJ477839.1)	97%
	YC-2-1	Embellisia sp. (JN859366.1)	99%
	YC-3	Phialocephala fluminis (AF486124.1)	100%
沙坡头 Shapotou	SPT-1	Phoma medicaginis (FJ224118.1)	99%