Chin J Plant Ecol ›› 2019, Vol. 43 ›› Issue (2): 107-118.doi: 10.17521/cjpe.2018.0272

• Research Articles • Previous Articles     Next Articles

Effects of simulated warming and decomposition interface on the litter decomposition rate of Zizania latifolia and its phyllospheric microbial community structure and function

YAN Peng-Fei1,ZHAN Peng-Fei1,XIAO De-Rong1,WANG Yi2,YU Rui1,LIU Zhen-Ya1,WANG Hang1,*()   

  1. 1 Southwest Forestry University National Plateau Wetlands Research Center/Wetlands College, Kunming 650224, China
    2 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China
  • Received:2018-10-31 Accepted:2019-01-30 Online:2019-06-04 Published:2019-02-20
  • Contact: WANG Hang
  • Supported by:
    Supported by the National Natural Science Foundation of China(41877346);Supported by the National Natural Science Foundation of China(31500409);Supported by the National Natural Science Foundation of China(41867059)

Abstract: <i>Aims</i>

Litters of emergent plants are important components of material cycling in wetland ecosystems. To clarify the effects of climate warming and habitat difference on the litter decomposition processes and phyllospheric microorganisms of wetland emergent plants is of great significance for revealing the key material cycling processes in wetland ecosystems.


Zizania latifolia, a dominant emergent plant in typical wetlands of Northwestern Yunnan Plateau, was chosen for this study. Using litter bag methods, we studied mass remaining and the abundance, community structure and metabolic potential of phyllospheric microorganisms of the litter from Zizania latifolia under simulated warming (1.5-2.0 ℃) and under three habitats (air, water and soil interface).

<i>Important findings</i>

Simulated climatic warming and habitat difference significantly affected the litter decomposition rate. After one-year decomposition, the mass remaining of litter was 66.4% under the simulated warming treatment, while 77.7% under the control treatment. The decomposition constant (k) value was 1.64 times under warming compared to the control. The mass remaining of litter at the water and soil interface was 42.2% and 25.3%, and the k value at the water and soil interface was 3.63 and 5.25 times of that at the air interface respectively. These results indicate that habitat difference was the key factor controlling the decomposition of emergent plant litter in wetlands. Moreover, warming mainly changed the community composition of litter phyllospheric microorganisms, while decomposition interface mainly affected the abundance, community structure and metabolic potential of phyllospheric microorganisms. Notably, phyllospheric microorganisms of litter at soil interface had the highest metabolic potential and utilized alcohols as main carbon sources. The characteristics of phyllospheric microorganisms between different treatments were in good agreement with litter decomposition rate, which provides an important theoretical basis for revealing the microbial mechanisms driving the decomposition of wetland plant litter.

Key words: wetland ecosystem, litter decomposition, phyllospheric microorganisms, simulated warming, habitat difference

Fig. 1

Experiment of simulated warming and habitat difference for litter decomposition of Zizania latifolia. A, Three habitats include air interface, water interface, and soil interface. Among them, litter bags under air decomposition were hang over the bamboo (1.2 m from the ground), litter bags under water decomposition were floated in the surface of water (with the aids of table tennis), and litter bags under soil decomposition were fixed by PVC tubes in the soils (5.0 cm in deep). B, The design and operation of Open-top Chamber (OTC). Among them, control group has no OTC devices, and OTC devices simulate rising temperature (warming group). The device was constructed by solar panels with 2.4 m base and 2.0 m opening in diameter. The temperatures between control and warming groups were recorded from December 2014 to December 2015 (once per hour). In warming treatment, the temperature has been raised by 1.5-2.0 ℃. C, The research object was a typical emergent wetland plant, Zizania latifolia. Its leaf litter was subjected to warming and habitat difference treatments."

Fig. 2

Seasonal dynamics in mass remaining of leaf litter from Zizania latifolia (mean ± SE, n = 3). The different lowercase letters above error bars indicate significant differences between treatments by Post Hoc Tests (p < 0.05)."

Fig. 3

Microbial colony counts in culture dish for leaf litter of Zizania latifolia (mean ± SE). *, p < 0.05; **, p < 0.01."

Fig. 4

Diversity of bacterial community indicated by Chao1 index and the bacterial community composition at the genus level for leaf litter of Zizania latifolia. The error bars represent standard errors (n = 3), and the different lowercase letters above error bars indicate significant differences between treatments by Post Hoc Tests (p < 0.05)."

Fig. 5

Dynamics in average well color development (AWCD) value for carbon sources utilized by litter phyllospheric microorganisms of Zizania latifolia during an incubation period of 12-168 h. The error bars represent standard errors (n = 3), and the different lowercase letters above error bars indicate significant differences between treatments by Post Hoc Tests (p < 0.05)."

Fig. 6

Utilization of six major groups of carbon sources by litter phyllospheric microorganisms of Zizania latifolia."

Fig. 7

Similarity analysis shows the contribution of different carbon sources to the dissimilarity between control vs. warming, air interface vs. water interface, air interface vs. soil interface, and water interface vs. soil interface, illustrated by heatmaps. The color (blue to red) represents the relative contribution of different carbon substrates (0-100%). The observations at 72-96 h incubation point were used for drawing the heatmaps."

[1] Berg B, McClaugherty C ( 1989). Nitrogen and phosphorus release from decomposing litter in relation to the disappearance of lignin. Canadian Journal of Botany, 67, 1148-1156.
doi: 10.1139/b89-150
[2] Bonanomi G, Capodilupo M, Incerti G, Mazzoleni S ( 2015). Litter quality and temperature modulate microbial diversity effects on decomposition in model experiments. Community Ecology, 16, 167-177.
doi: 10.1139/b89-150
[3] Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello KE, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelly ST, Knights D, Koening JE, Ley RE, Lozupone GA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Rob K ( 2010). QIIME allows analysis of high-throghput community sequencing data. Nature, 7, 335-336.
doi: 10.1038/nmeth.f.303
[4] Chaudhary D, Kumar R, Sihag K, Rashmi & Kumari A ( 2017). Phyllospheric microflora and its impact on plant growth: A review. Agricultural Reviews, 38, 51-59.
[5] Chen C, Xin K, Liu H, Cheng J, Shen XH, Wang Y, Zhang L ( 2017). Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat. Scientific Reports, 7, 41564. DOI: 10.1038/srep41564.
doi: 10.1038/srep41564
[6] Chen YM, He RL, Deng CC, Yang WQ, Zhang J, Yang L, Liu Y ( 2015). Litter decomposition and lignocellulose enzyme activities of Actinothuidium hookeri and Cystopteris montana in alpine timberline ecotone of Western Sichuan, China. Chinese Journal of Applied Ecology, 26, 3251-3258.
doi: 10.3321/j.issn:0412-1961.2006.02.002 pmid: 26915177
[ 陈亚梅, 和润莲, 邓长春, 杨万勤, 张健, 杨林, 刘洋 ( 2015). 川西高山林线交错带两种地被物分解的木质纤维素酶活性特征. 应用生态学报, 26, 3251-3258.]
doi: 10.3321/j.issn:0412-1961.2006.02.002 pmid: 26915177
[7] Christoffer B, Mireia BF, Jarone P, Catherine L ( 2018). Response of microbial communities to changing climate conditions during summer cyanobacterial blooms in the Baltic Sea. Frontiers in Microbiology, 9, 1562. DOI: 10.3389/ fmicb.2018.01562.
doi: 10.3389/fmicb.2018.01562
[8] Fan ZX, Bräuning A, Thomas A, Li JB, Cao KF ( 2011). Spatial and temporal temperature trends on the Yunnan Plateau (Southwest China) during 1961-2004. International Journal of Climatology, 31, 2078-2090.
doi: 10.1002/joc.2214
[9] Ferreira V, Raposeiro PM, Pereira A, Cruz AM, Costa AC, Graca M, Goncalves V ( 2016). Leaf litter decomposition in remote oceanic island streams is driven by microbes and depends on litter quality and environmental conditions. Freshwater Biology, 61, 783-799.
doi: 10.1111/fwb.12749
[10] Guo XH, Xiao DR, Tian K, Yu HZ ( 2013). Biomass production and litter decomposition of lakeshore plants in Napahai wetland, Northwestern Yunnan Plateau, China. Acta Ecologica Sinica , 33, 1425-1432.
doi: 10.5846/stxb201208271209
[ 郭绪虎, 肖德荣, 田昆, 余红忠 ( 2013). 滇西北高原纳帕海湿地湖滨带优势植物生物量及其凋落物分解. 生态学报, 33, 1425-1432.]
doi: 10.5846/stxb201208271209
[11] He XB, Song FQ, Zhang P, Lin YH, Tian XJ, Ren LL, Chen CL, Xiao N, Tan HX ( 2007). Variation in litter decomposition- temperature relationships between coniferous and broadleaf forests in Huangshan Mountain, China. Journal of Forestry Research, 18, 291-297.
doi: 10.1007/s11676-007-0058-0
[12] Hobara S, Osono T, Hirose D, Noro K, Hirota M, Benner R ( 2014). The roles of microorganisms in litter decomposition and soil formation. Biogeochemistry, 118, 471-486.‌
doi: 10.1007/s10533-013-9912-7
[13] Huang JX, Huang LM, Lin ZC, Chen GS ( 2010). Controlling factors of litter decomposition rate in China’s forests. Journal of Subtropical Resources and Environment , 5(3), 56-63.
doi: 10.3969/j.issn.1673-7105.2010.03.008
[ 黄锦学, 黄李梅, 林智超, 陈光水 ( 2010). 中国森林凋落物分解速率影响因素分析. 亚热带资源与环境学报, 5(3), 56-63.]
doi: 10.3969/j.issn.1673-7105.2010.03.008
[14] IPCC(Intergovernmental Panel on Climate Change)( 2013). Climate Change 2013: The Physical Science Basis. Cambridge University Press, Cambridge, UK.
doi: 10.1007/BF00524943
[15] Kuehn KA, Steiner D, Gessner MO ( 2004). Diel mineralization patterns of standing-dead plant litter: Implications for CO2 flux from wetlands. Ecology, 85, 2504-2518.
doi: 10.1890/03-4082
[16] Kurten GL, Barkoh A ( 2016). Evaluation of community-level physiological profiling for monitoring microbial community function in aquaculture ponds. North American Journal of Aquaculture, 78, 34-44.
doi: 10.1080/15222055.2015.1079580
[17] Li Q, Zhou DW, Chen XY ( 2014). The accumulation, decomposition and ecological effects of above-ground litter in terrestrial ecosystem. Acta Ecologica Sinica , 34, 3807-3819.
[ 李强, 周道玮, 陈笑莹 ( 2014). 地上枯落物的累积、分解及其在陆地生态系统中的作用. 生态学报, 34, 3807-3819.]
[18] Li SS, Wang ZW, Yang JJ ( 2016). Changes in soil microbial communities during litter decomposition. Biodiversity Science , 24, 195-204.
[ 李姗姗, 王正文, 杨俊杰 ( 2016). 凋落物分解过程中土壤微生物群落的变化. 生物多样性, 24, 195-204.]
[19] Liu GF, Cornwell WK, Pan X, Ye D, Liu FH, Huang ZY, Dong M, Cornelissen JH ( 2015). Decomposition of 51 semidesert species from wide-ranging phylogeny is faster in standing and sand-buried than in surface leaf litters: Implications for carbon and nutrient dynamics. Plant and Soil, 396, 175-187.
[20] Marty C, Houle D, Gagnon C ( 2015). Variation in stocks and distribution of organic C in soils across 21 eastern Canadian temperate and boreal forests . Forest Ecology and Management, 345, 29-38.
[21] Moghadam FS, Zimmer M ( 2014). Effects of warming and nutrient enrichment on how grazing pressure affects leaf litter-colonizing bacteria. Journal of Environment Quality, 43, 851-858.
[22] Newell SY ( 2001). Fungal biomass and productivity in‌ standing-decaying leaves of black needlerush (Juncus roemerianus). Marine & Freshwater Research, 52, 249-255.
[23] Ni XY, Yang WQ, Li H, Xu LY, He J, Tan B, Wu FZ ( 2017). The responses of early foliar litter humification to reduced snow cover during winter in an alpine forest. Canadian Journal of Science, 94, 453-461.
[24] Parker TC, Sanderman J, Holden RD, Blume-Werry G, Sjogersten S, Large D, Castro-Diaz M, Street LE, Subke JA, Wookey PA ( 2018). Exploring drivers of litter decomposition in a greening Arctic: Results from a transplant experiment across a tree-line. Ecology, 99, 2284-2294.
[25] Peng SL, Liu Q ( 2002). The dynamics of forest litter and its responses to global warming. Acta Ecologica Sinica , 22, 1534-1544.
[ 彭少麟, 刘强 ( 2002). 森林凋落物动态及其对全球变暖的响应. 生态学报, 22, 1534-1544.]
[26] Saura-Mas S, Estiarte M, Peñuelas J, Lloret F ( 2012). Effects of climate change on leaf litter decomposition across post-fire plant regenerative groups. Environmental & Experimental Botany, 77, 274-282.
[27] Song P, Zhang NL, Ma KP, Guo JX ( 2014). Impacts of global warming on litter decomposition. Acta Ecologica Sinica , 34, 1327-1339.
[ 宋飘, 张乃莉, 马克平, 郭继勋 ( 2014). 全球气候变暖对凋落物分解的影响. 生态学报, 34, 1327-1339.]
[28] Song XZ, Jiang H, Zhang HL, Yu SQ, Zhou GM, Ma YD, Chang SX ( 2008). A review on the effects of global environment change on litter decomposition. Acta Ecologica Sinica , 28, 4414-4423.
[ 宋新章, 江洪, 张慧玲, 余树全, 周国模, 马元丹, Scott X. Chang ( 2008). 全球环境变化对森林凋落物分解的影响. 生态学报, 28, 4414-4423.]
[29] Song Y, Gu XR, Yan HY, Mao WT, Wu XL, Wan YX ( 2014). Dynamics of microbes and enzyme activities during litter decomposition of Pinus Massoniana forest in mid-subtropical area. Environmental Science, 35, 1151-1158.
[ 宋影, 辜夕容, 严海元, 毛文韬, 吴雪莲, 万宇轩 ( 2014). 中亚热带马尾松林凋落物分解过程中的微生物与酶活性动态. 环境科学, 35, 1151-1158.]
[30] Sørensen J, Nybroe O ( 2004). Pseudomonas. Springer, Boston, USA. 303-324.
[31] Sun ZG, Liu JS ( 2007). Development in study of wetland litter decomposition and its responses to global change. Acta Ecologica Sinica , 27, 1606-1618.
[ 孙志高, 刘景双 ( 2007). 湿地枯落物分解及其对全球变化的响应. 生态学报, 27, 1606-1618.]
[32] Tang SS, Yang WQ, Yin R, Xiong L, Wang HP, Wang B, Zhang Y, Peng YJ, Chen QS ( 2014). Spatial characteristics in decomposition rate of foliar litter and controlling factors in Chinese forest ecosystems. Chinese Journal of Plant Ecology , 38, 529-539.
[ 唐仕姗, 杨万勤, 殷睿, 熊莉, 王海鹏, 王滨, 张艳, 彭艳君, 陈青松 ( 2014). 中国森林生态系统凋落叶分解速率的分布特征及其控制因子. 植物生态学报, 38, 529-539.]
[33] Veronica F, Chauvet E ( 2011). Future increase in temperature more than decrease in litter quality can affect microbial litter decomposition in streams. Oecologia, 167, 279-291.
[34] Wang FY ( 1989). Forest litter quality research review. Advances in Ecology , 2, 82-89.
[ 王凤友 ( 1989). 森林凋落量研究综述. 生态学进展, 2, 82-89.]
[35] Wang YH, Gong JR, Liu M, Huang YM, Yan X, Zhang ZY, Xu S, Luo QP ( 2015). Effects of grassland-use on soil respiration and litter decomposition. Chinese Journal of Plant Ecology , 39, 239-248.
[ 王忆慧, 龚吉蕊, 刘敏, 黄永梅, 晏欣, 张梓瑜, 徐沙, 罗亲普 ( 2015). 草地利用方式对土壤呼吸和凋落物分解的影响. 植物生态学报, 39, 239-248.]
[36] Wang Z, Cao GQ, Zhang YQ, Zhang HY, Wang F, Chen AL ( 2017). Responses of metabolism diversity of topsoil microbial to the litterfall addition in Cunninghamia laneolata plantation. Journal of Forest and Environment, 37, 148-154.
[ 王珍, 曹光球, 张月全, 张海燕, 王飞, 陈爱玲 ( 2017). 凋落物配比对杉木土壤微生物碳代谢多样性的影响. 森林与环境学报, 37, 148-154.]
[37] Warskow AL, Juni E ( 1972). Nutritional requirements of Acinetobacter strains isolated from soil, water, and sewage. Journal of Bacteriology, 112, 1014-1016.
[38] Wierzchos J, Casero MC, Artieda O, Ascaso C ( 2018). Endolithic microbial habitats as refuges for life in polyextreme environment of the Atacama Desert. Current Opinion in Microbiology, 43, 124-131.
[39] Wu FZ, Yang WQ, Zhang J, Deng RJ ( 2010). Litter decomposition in two subalpine forests during the freeze-thaw season. Acta Oecologica, 36, 135-140.
[40] Xu XF, Tian HQ, Wan SQ ( 2007). Climate warming impacts on carbon cycling in terrestrial ecosystems. Journal of Plant Ecology (Chinese Version), 31, 175-188.
[ 徐小峰, 田汉勤, 万师强 ( 2007). 气候变暖对陆地生态系统碳循环的影响. 植物生态学报, 31, 175-188.]
[41] Xu ZF, Yin HJ, Zhao CZ, Cao G, Wan ML, Liu Q ( 2009). A review of response of litter decomposition in terrestrial ecosystems to global warming. Journal of Plant Ecology , 33, 1208-1219.
[ 徐振锋, 尹华军, 赵春章, 曹刚, 万名利, 刘庆 ( 2009). 陆地生态系统凋落物分解对全球气候变暖的响应. 植物生态学报, 33, 1208-1219.]
[42] Yang WQ, Deng RJ, Zhang J ( 2007). Forest litter decomposition and its responses to global climate change. Chinese Journal of Applied Ecology , 18, 2889-2895.
[ 杨万勤, 邓仁菊, 张健 ( 2007). 森林凋落物分解及其对全球气候变化的响应. 应用生态学报, 18, 2889-2895.]
[43] Yang XH, Peng LJ, Li SY, Wang SZ ( 2013). Effect of mangrove leaf litter decomposition on soil dissolved organic matter. Ecology and Environmental Sciences , 22, 924-930.
[ 杨秀虹, 彭琳婧, 李适宇, 王诗忠 ( 2013). 红树植物凋落叶分解对土壤可溶性有机质的影响. 生态环境学报, 22, 924-930.]
[44] Yue K, Yang WQ, Peng Y, Huang CP, Zhang C, Wu FZ ( 2016). Effects of streams on lignin degradation during foliar litter decomposition in an alpine forest. Chinese Journal of Plant Ecology , 40, 893-901.
[ 岳楷, 杨万勤, 彭艳, 黄春萍, 张川, 吴福忠 ( 2016). 高寒森林溪流对凋落叶分解过程中木质素降解的影响. 植物生态学报, 40, 893-901.]
[45] Zhang J, Zhai FF, Zhang JH, Sun FB, Zhang HJ, Mao ZG ( 2010). The behavior of anaerobic fermentation in the technique of alcohol fermentation cooperate with methane fermentation. Journal of Food Science & Biotechnology , 29, 276-281.
[ 张静, 翟芳芳, 张建华, 孙付保, 张宏健, 毛忠贵 ( 2010). 酒精沼气双发酵偶联中厌氧沼气的发酵行为. 食品与生物技术学报, 29, 276-281.]
[46] Zhang XN, Liu ZY, Li LP, Wang H, Zhang Y, Sun M, Xiao DR ( 2017). Effect of experimental warming on the decomposition of litter from dominant lakeside plants in a typical wetland of Northwestern Yunnan Plateau, China. Acta Ecologica Sinica , 37, 7811-7820.
[ 张晓宁, 刘振亚, 李丽萍, 王行, 张贇, 孙梅, 肖德荣 ( 2017). 大气增温对滇西北高原典型湿地湖滨带优势植物凋落物质量衰减的影响. 生态学报, 37, 7811-7820.]
[47] Zheng H, Ouyang ZY, Fang ZG, Zhao TQ ( 2004). Application of BIOLOG to study on soil microbial community functional diversity. Acta Pedologica Sinica , 41, 456-461.
[ 郑华, 欧阳志云, 方治国, 赵同谦 ( 2004). BIOLOG在土壤微生物群落功能多样性研究中的应用. 土壤学报, 41, 456-461.]
[1] Ying Chen. Techniques and methods for field warming manipulation experiments in terrestrial ecosystems [J]. Chin J Plant Ecol, 2020, 44(生态技术与方法专辑): 0-0.
[2] JIA Bing-Rui. Litter decomposition and its underlying mechanisms [J]. Chin J Plant Ecol, 2019, 43(8): 648-657.
[3] SONG Xiao-Yan,WANG Gen-Xu,RAN Fei,YANG Yan,ZHANG Li,XIAO Yao. Flowering phenology and growth of typical shrub grass plants in response to simulated warmer and drier climate in early succession Taiga forests in the Da Hinggan Ling of northeast China [J]. Chin J Plan Ecolo, 2018, 42(5): 539-549.
[4] Wen-Jing CHEN, Lu GONG, Yu-Tong LIU. Effects of seasonal snow cover on decomposition and carbon, nitrogen and phosphorus release of Picea schrenkiana leaf litter in Mt. Tianshan, Northwest China [J]. Chin J Plan Ecolo, 2018, 42(4): 487-497.
[5] WU Qi-Qian, WANG Chuan-Kuan. Dynamics in foliar litter decomposition for Pinus koraiensis and Quercus mongolica in a snow-depth manipulation experiment [J]. Chin J Plan Ecolo, 2018, 42(2): 153-163.
[6] Li-Li YANG, Ji-Rui GONG, Min LIU, Bo YANG, Zi-He ZHANG, Qin-Pu LUO, Zhan-Wei ZHAI, Yan PAN. Advances in the effect of nitrogen deposition on grassland litter decomposition [J]. Chin J Plan Ecolo, 2017, 41(8): 894-913.
[7] Yu-Peng LU, Ji-Yuan XU, Xiao-Xi ZHANG, Bo-Ya WANG, Bo XIE, Zeng-Wen LIU. Effects of leachate from understory medicinal plants on litter decomposition and soil enzyme activities of Betula albo-sinensis and Eucommia ulmoides [J]. Chin J Plan Ecolo, 2017, 41(6): 639-649.
[8] Li-Li YANG, Ji-Rui GONG, Yi-Hui WANG, Min LIU, Qin-Pu LUO, Sha XU, Yan PAN, Zhan-Wei ZHAI. Effects of grazing intensity and grazing exclusion on litter decomposition in the temperate steppe of Nei Mongol, China [J]. Chin J Plan Ecolo, 2016, 40(8): 748-759.
[9] Shanshan Li,Zhengwen Wang,Junjie Yang. Changes in soil microbial communities during litter decomposition [J]. Biodiv Sci, 2016, 24(2): 195-204.
[10] WANG Yi-Hui,GONG Ji-Rui,LIU Min,HUANG Yong-Mei,YAN Xin,ZHANG Zi-Yu,XU Sha,LUO Qin-Pu. Effects of grassland-use on soil respiration and litter decomposition [J]. Chin J Plan Ecolo, 2015, 39(3): 239-248.
[11] HE Jie, YANG Wan-Qin, NI Xiang-Yin, LI Han, XU Li-Ya, and WU Fu-Zhong. Effects of snow patch on the dynamics of potassium and sodium during litter decomposition in winter in a subalpine forest of western Sichuan [J]. Chin J Plan Ecolo, 2014, 38(6): 550-561.
[12] WU Qi-Qian, WU Fu-Zhong, YANG Wan-Qin, XU Zhen-Feng, HE Wei, HE Min, ZHAO Ye-Yi, and ZHU Jian-Xiao. Effect of seasonal snow cover on litter decomposition in alpine forest [J]. Chin J Plan Ecolo, 2013, 37(4): 296-305.
[13] HE Wei, WU Fu-Zhong, YANG Wan-Qin, WU Qi-Qian, HE Min, and ZHAO Ye-Yi. Effect of snow patches on leaf litter mass loss of two shrubs in an alpine forest [J]. Chin J Plan Ecolo, 2013, 37(4): 306-316.
[14] LIU Rui-Long, YANG Wan-Qin, TAN Bo, WANG Wen-Jun, NI Xiang-Yin, and WU Fu-Zhong. Effects of soil fauna on N and P dynamics at different stages during the first year of litter decomposition in subalpine and alpine forests of western Sichuan [J]. Chin J Plan Ecolo, 2013, 37(12): 1080-1090.
[15] TU Li-Hua, HU Hong-Ling, HU Ting-Xing, ZHANG Jian, LUO Shou-Hua, and DAI Hong-Zhong. Response of Betula luminifera leaf litter decomposition to simulated nitrogen deposition in the Rainy Area of West China [J]. Chin J Plan Ecolo, 2012, 36(2): 99-108.
Full text



[1] . [J]. Chin Bull Bot, 1994, 11(专辑): 19 .
[2] Xiao Xiao and Cheng Zhen-qi. Chloroplast 4.5 S ribosomol DNA. II Gene and Origin[J]. Chin Bull Bot, 1985, 3(06): 7 -9 .
[3] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chin Bull Bot, 2003, 20(03): 319 -324 .
[4] SONG Li-Ying TAN Zheng GAO Feng DENG Shu-Yan. Advances in in vitro Culture of Cucurbitaceae in China[J]. Chin Bull Bot, 2004, 21(03): 360 -366 .
[5] . [J]. Chin Bull Bot, 1994, 11(专辑): 76 .
[6] LI Jun-De YANG Jian WANG Yu-Fei. Aquatic Plants in the Miocene Shanwang Flora[J]. Chin Bull Bot, 2000, 17(专辑): 261 .
[7] XU Jing-Xian WANG Yu-Fei YANG Jian PU Guang-Rong ZHANG Cui-Fen. Advances in the Research of Tertiary Flora and Climate in Yunnan[J]. Chin Bull Bot, 2000, 17(专辑): 84 -94 .
[8] Sun Zhen-xiao Xia Guang-min Chen Hui-min. Karyotype Analysis of Psathyrostachys juncea[J]. Chin Bull Bot, 1995, 12(01): 56 .
[9] . [J]. Chin Bull Bot, 1994, 11(专辑): 8 -9 .
[10] Yunpu Zheng;Jiancheng Zhao * ;Bingchang Zhang;Lin Li;Yuanming Zhang . Advances on Ecological Studies of Algae and Mosses in Biological Soil Crust[J]. Chin Bull Bot, 2009, 44(03): 371 -378 .