Chin J Plan Ecolo ›› 2013, Vol. 37 ›› Issue (6): 530-541.doi: 10.3724/SP.J.1258.2013.00054

• Research Articles • Previous Articles     Next Articles

Responses of photosynthetic characteristics of Stipa baicalensis to grazing disturbance

YAN Xin, GONG Ji-Rui*, ZHANG Zi-Yu, HUANG Yong-Mei, AN Ran, QI Yu, and LIU Min   

  1. State Key Laboratory of Surface Processes and Resource Ecology, College of Resources Science and Technology, Beijing Normal University, Beijing 100875, China
  • Received:2012-11-13 Revised:2013-02-06 Online:2013-06-05 Published:2013-06-01
  • Contact: GONG Ji-Rui


Aims Our objective was to evaluate (a) the impact of livestock grazing disturbance on physiological traits of Stipa baicalensis and (b) the adaptive mechanisms that S. baicalensis employed.
Methods We investigated the diurnal variations of photosynthetic characteristics, water relations, light energy utilization, photosynthetic apparatus activity and osmotic regulation of S. baicalensis growing in both grazing and enclosed grassland of Hulun Buir, Inner Mongolia.
Important findings Both ribulose-1,5-bisphosphate carboxylase (RUBPCase) and chlorophyll content increased and the direction of electronic chain was altered with grazing, which is beneficial for S. baicalensis to cope with the impact of grazing disturbance by accumulating more assimilates. The midday depression of photosynthesis of S. baicalensis in the enclosed grassland resulted from the decline of RUBPCase carboxylation capacity, which is due to the absence of P. The photochemical quenching (qP) of S. baicalensis in the grazed grassland is higher, which reflects that it tended to use more absorbed solar energy to improve photosynthetic capacity, which was adaptive to the effects of grazing. Lower leaf water content of S. baicalensis in the grazed field led to the increase of osmotic regulating substances for obtaining sufficient water from the soil. In addition, grazing disturbance promoted the absorption and utilization of N by S. baicalensis. Negative correlation of leaf mass per area (LAM) with photosynthetic nitrogen-use efficiency (PNUE) and photosynthetic phosphorus-use efficiency (PPNE) was observed in the S. baicalensis in both grasslands. Our research demonstrated that various ecophysiological mechanisms were employed by S. baicalensis as adaptive to grazing.

[1]Anderson JM (1986). Photo regulation of the composition, function, and structure of thylakoid membranes. Annual Review of Plant Physiology, 37, 93–136. Crossref
[2] Anderson TM, Dong Y, McNaughton SJ (2006). Nutrient acquisition and physiological responses of dominant Serengeti grasses to variation in soil texture and grazing. Journal of Ecology, 94, 1164–1175. Crossref
[3] Arnon D (1949). Copper enzymes in isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1–25. Crossref
[4] Asada K (1994). Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH, Mullineaux PM eds. Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants. CRC Press, Tokyo. 77–104. Crossref
[5] Bieleski RL (1973). Phosphate pools, phosphate transport, and phosphate availability. Annual Review of Plant Physiology, 24, 225–252. Crossref
[6] Chen SP, Bai YF, Lin GH, Liang Y, Han XG (2005). Effects of grazing on photosynthetic characteristics of major steppe species in the Xilin River Basin, Inner Mongolia, China. Photosynthetica, 43, 559–565. Crossref
[7] Chen ZZ, Wang SP (2000). Typical Grassland Ecosystem of China. Science Press, Beijing. (in Chinese) [陈佐忠, 汪诗平 (2000). 中国典型草原生态系统. 科学出版社, 北京.] Crossref
[8] Crofts AR, Baroli I, Kramer D, Taoka S (1993). Kinetics of electron transfer between QA and QB in wild type and herbicide-resistant mutants of Chlamydomonas reinhardtii. Z Naturforsch, 48, 259–266. Crossref
[9] Demmig B, Björkman O (1987). Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. Planta, 171, 171–184. Crossref
[10] Demmig-Adams B, Adams WW III, Barker DH, Logan BA, Bowling DR, Verhoeven AS (1996). Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. Physiologia Plantarum, 98, 253–264. Crossref
[11] Elser JJ, Marzolf ER, Goldman CR (1990). Phosphorus and nitrogen limitation of phytoplankton growth in the freshwaters of North America: a review and critique of experimental enrichments. Canadian Journal of Fisheries and Aquatic Sciences, 47, 146–1477. Crossref
[12] Epron D, Godard D, Cornic G, Genty B (1995). Limitation of net CO2 assimilation rate by internal resistance to CO2 transfer in the leaves of two tree species (Fagus sylvation L. and Castanea sativa Mill.). Plant, Cell & Environment, 18, 43–51. Crossref
[13] Evans JR (1983). Nitrogen and photosynthesis in the flagleaf of wheat (Triticum aestivum L.). Plant Physiology, 72, 297–302. Crossref
[14] Evans JR, Seemann JR (1984). Differences between wheat genotypes in specific activity of ribulose-1, 5 bisphos- phate carboxylase and the relationship to photosynthesis. Plant Physiology, 74, 759–765. Crossref
[15] Farquhar GD, Sharkey TD (1982). Stomach conductance and photosynthesis. Annual Review of Plant Physiology, 33, 317–345. Crossref
[16] Farquhar GD, von Caemmerer S, Berry JA (1980). A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta, 149, 78–90. Crossref
[17] Filella I, Llusià J, Piñol J, Peñuelas J (1998). Leaf gas exchange and fluorescence of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions. Environmental and Experimental Botany, 39, 213–220. Crossref
[18] Groat RG, Vance CP (1981). Root nodule enzymes of ammonia assimilation in alfalfa (Medicago sativa L). Plant Physiology, 67, 1198–1203. Crossref
[19] Hikosaka K (2004). Interspecific difference in the photosynthesis-nitrogen relationship: patterns, physiological causes, and ecological importance. Journal of Plant Research, 117, 481–494. Crossref
[20] Kedrowski RA (1983). Extraction and analysis of nitrogen, phosphorus and carbon fractions in plant material. Journal of Plant Nutrition, 6, 989–1011. Crossref
[21] Krall JP, Edward GE (1992). Relationship between photosystem II activity and CO2 fixation in leaves. Physiologia Plantarum, 86, 180–187. Crossref
[22] Kramer PJ (1998). Water Relations of Plants. Academic Press, New York. Crossref
[23] Laisk A, Loreto F (1996). Determining photosynthetic parameters from leaf CO2 exchange and chlorophyll fluorescence. Plant Physiology, 110, 903–912. Crossref
[24] Larcher W (1994). Ökophysiologie der Pflanzen. Verlag Eugen Ulmer, Stuttgart. 47–100. Crossref
[25] Li HS (1999). Plant Physiological Biochemical Experiment Principles and Techniques. Higher Education Press, Beijing. 131–261. (in Chinese) [李合生 (1999). 植物生理生化实验原理和技术. 高等教育出版社, 北京. 131–261.] Crossref
[26] Liang EY, Wang YF, Xu Y, Liu B, Shao XM (2010). Growth variation in Abies georgei var. smithii along altitudinal gradients in the Sygera Mountains, southeastern Tibetan. Plateau Trees, 24, 363–373. Crossref
[27] Lin ZF, Peng CL (2000). The allocation of photosynthetic electron transport and absorbed light energy in leaves of four woody plants acclimated to different light intensities. Acta Phytophysiologica Sinica, 26, 387–392. (in Chinese with English abstract) [林植芳, 彭长连 (2000). 4种木本植物叶片的光合电子传递和吸收光能分配特性对光强的适应. 植物生理学报, 26, 387–392.] Crossref
[28] Makhnev AK, Makhneva NE (2010). Landscape-ecological and population aspects of the strategy of restoration of disturbed lands. Contemporary Problems of Ecology, 3, 318–322. Crossref
[29] Marschner H (1995). Mineral Nutrition of Higher Plants. 2nd edn. Academic Press, London, UK. Crossref
[30] Melis A, Harvey GW (1981). Regulation of photosystem stoichiometry, chlorophyll a and chlorophyll b content and relation to chloroplast ultrastructure. Biochimica et Biophysica Acta-Bioenergetics, 63, 138–145. Crossref
[31] Meyer GA (1998). Mechanisms promoting recovery from defoliation in goldenrod (Solidago altissima). Canadian Journal of Botany, 76, 450–459. Crossref
[32] Onoda HK, Hirose T, Hirose T (2004). Allocation of nitrogen to cell walls decreases photosynthetic nitrogen-use efficiency. Functional Ecology, 18, 419–425. Crossref
[33] Parkhurst DF (1994). Diffusion of CO2 and other gases inside leaves. New Phytologist, 126, 449–479. Crossref
[34] Peng Y, Jiang GM, Liu XH, Niu SL, Liu MZ, Biswas DK (2007). Photosynthesis, transpiration and water use efficiency of four plant species with grazing intensities in Hunshandak Sandland, China. Journal of Arid Environments, 70, 304–315. Crossref
[35] Poorter H, Evans JR (1998). Photosynthetic nitrogen-use efficiency of species that differ inherently in specific leaf area. Oecologia, 116, 126–137. Crossref
[36] Racker E (1962). Ribulose diphosphate carboxylase from spinach leaves. In: Colowick SP, Kaplan NO eds. Methods in Enzymology. Academic Press, New York. 266–278. Crossref
[37] Richards JH (1993). Physiology of plants recovering from defoliation. In: Proceedings of the XVII International Grasslands Conference. Polmerston North, New Zealand. 47–55. Crossref
[38] Sukenik A, Bennett J, Falkowski P (1987). Light-saturated photosynthesis-limitation by electron transport or carbon fixation. Biochimica et Biophysica Acta-Bioenergetics, 2, 205–215. Crossref
[39] Sun W, Resco V, Williams DG (2010). Nocturnal and seasonal patterns of carbon isotope composition of leaf dark- respired carbon dioxide differ among dominant species in a semiarid savanna. Oecologia, 164, 297–310. Crossref
[40] Takashhna T, Kkosaka K, Hirose T (2004). Photosynthesis or persistence: nitrogen allocation in leaves of evergreen and deciduous Quercus species. Plant, Cell & Environment, 27, 1047–1054. Crossref
[41] Vu JC, Allon LH, Gowes G (1987). Drought stress and elevated CO2 effects on soybean ridulose bisphosphate carboxylase activity and canopy photosynthetic rates. Plant Physiology, 83, 573–578. Crossref
[42] Xue S, Wang PH (1992). Effects of water stress of CO2 assimilation of two winter wheat cultivars with different drought resistance. Acta Phytophysiol Sinica, 18, 1–7. (in Chinese with English abstract) Crossref
[43] Ye JY, Mi HL (1999). Experimental Guide of Modern Plant Physiology. Science Press, Beijing. 108–109. (in Chinese) [叶济宇, 米华玲 (1999). 现代植物生理学实验指南. 科学出版社, 北京. 108–109.] Crossref
[44] Zhao P, Sun GC, Peng SL (1998). Ecophysiological research on nitrogen nutrition of plant. Ecologic Science, 17, 37–42. (in Chinese with English abstract) [赵平, 孙谷畴, 彭少麟 (1998). 植物氮素营养的生理生态学研究. 生态科学, 17, 37–42.] Crossref
[45] Zhao W, Chen SP, Han XG, Lin GH (2009). Effects of long-term grazing on the morphological and functional traits of Leymus chinensis in the semiarid grassland of Inner Mongolia, China. Ecological Research, 24, 99–108. Crossref
[46] Zhou G, Wang Y, Wang S (2002). Responses of grassland ecosystems to precipitation and land use along the Northeast China Transect. Journal of Vegetation Science, 13, 361–368. Crossref
[47] Zou Q (2000). The Experiment Guide of Plant Physiology. China Agricultural Press, Beijing. 80–82, 137–138, 119–120. (in Chinese) [邹琦 (2000). 植物生理学实验指导. 中国农业科技出版社, 北京. 80–82, 137–138, 119–120.] Crossref
No related articles found!
Full text



[1] Liu De-li. Heat-Shock Proteins of Plants and their Functions[J]. Chin Bull Bot, 1996, 13(01): 14 -19 .
[2] Chengqiang Ding, Dan Ma, Shaohua Wang, Yanfeng Ding. Optimization Process and Method of 2-D Electrophoresis for Rice Proteomics[J]. Chin Bull Bot, 2011, 46(1): 67 -73 .
[3] SONG Ke-Min. Phosphorus Nutrition of Plants: Phosphate Transport Systems and their Regulation[J]. Chin Bull Bot, 1999, 16(03): 251 -256 .
[4] CHEN Fa-Ju;YANG Ying-Gen;ZHAO De-Xiu;GUI Yao-Lin and GUO Zhong-Chen. Advances in Studies of Species Habitats Distribution and Chemical Composition of Snow Lotuses(Saussurea) in China[J]. Chin Bull Bot, 1999, 16(05): 561 -566 .
[5] YANG Hong-QiangJIE Yu-lingLI Jun. The Stresses Messenger from Roots and Its Production and Transport in Plant[J]. Chin Bull Bot, 2002, 19(01): 56 -62 .
[6] Hui Li, Guangcan Zhang, Huicheng Xie, Jingwei Xu, Chuanrong Li, Juwen Sun. The Effect of Phenol Concentration on Photosynthetic Physiological Parameters of Salix babylonica[J]. Chin Bull Bot, 2016, 51(1): 31 -39 .
[7] . [J]. Chin Bull Bot, 1996, 13(专辑): 97 -98 .
[9] Wang Renqing. Comparative Study on the Vegetation Between Shandong and Liaodong Peninsulas[J]. Chin J Plan Ecolo, 1984, 8(1): 41 -51 .
[10] Ge Ying, Chang Jie, Lu Dagen, Yue Chunlei, Jiang Hong. A Study on the Ecological Characters of Mosla hangchowensis[J]. Chin J Plan Ecolo, 1999, 23(1): 14 -22 .