外生菌根真菌对青藏高原东缘岷江冷杉和糙皮桦氮获取贡献及其影响因素

doi:10.17521/cjpe.2025.0252

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外生菌根真菌对青藏高原东缘岷江冷杉和糙皮桦氮获取贡献及其影响因素

陈淼, 陈健, 刘顺, 许格希, 冯秋红, 史作民

中国林业科学研究院森林生态环境与自然保护研究所，国家林业和草原局森林生态环境重点实验室，北京 100091, 北京中国
四川米亚罗森林生态系统定位观测研究站，四川理县 623100, 北京中国
四川省林业科学研究院, 森林和湿地生态保护修复与资源挖掘利用四川省重点实验室, 成都 610081, 北京中国
四川大熊猫国家公园定位观测研究站, 卧龙森林生态四川省野外科学观测研究站, 四川阿坝 623006, 北京中国
南京林业大学南方现代林业协同创新中心，南京 210037, 北京中国

收稿日期:2025-07-02 修回日期:2025-12-31 接受日期:2025-12-16
基金资助:
项目基金：国家重点研发计划课题(2024YFD2201402); 中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2022SY021)

The contribution and influencing factors of ectomycorrhizal fungi to nitrogen acquisition for Abies fargesii var. faxoniana and Betula utilis on the eastern Qinghai–Tibetan Plateau

CHEN Miao, CHEN Jian, LIU Shun, XU Ge-xi, FENG Qiu-hong, SHI Zuo-min

, Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China , China
, Sichuan Miyaluo Forest Ecosystem Observation and Research Station, Lixian County, Sichuan 623100, China , China
, Ecological Conservation, Restoration and Resource Utilization on Forest and Wetland Key Laboratory of Sichuan Province, Sichuan Academy of Forestry, Chengdu 610081, China , China
, Sichuan Giant Panda National Park Observation and Research Station, Wolong Forest Ecology Observation and Research Station of Sichuan Province,, Aba, Sichuan 623006, China , China
, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China , China

Received:2025-07-02 Revised:2025-12-31 Accepted:2025-12-16
Supported by:
Supported by the National Key Research and Development Program of China(2023YFD2200404-03); Supported by the National Key Research and Development Program of China(2021YFD2200405); the Fundamental Research Funds of Chinese Academy of Forestry(CAFYBB2021ZA002-2); the Fundamental Research Funds of Chinese Academy of Forestry(CAFYBB2022QC002); the Sichuan Science and Technology Program(2024ZYD0169); Supported by the National Key Research and Development Program of China(and 2024YFD2201402); the Fundamental Research Funds of Chinese Academy of Forestry(and CAFYBB2022SY021)

摘要/Abstract

摘要： 外生菌根树木既通过细根直接吸收土壤中的氮(根途径)，也通过外生真菌菌丝吸收土壤氮(菌丝途径)后转移到细根上。然而，目前对于菌丝途径对外生菌根树木氮获取的相对贡献(f_fungi)的量化估算仍具有较大的不确定性，其关键影响因素亦不甚明确。本研究以青藏高原东缘岷江冷杉(Abies fargesii var. faxoniana)和糙皮桦(Betula utilis)为对象，采用¹⁵N自然丰度法量化了菌丝途径对其氮素获取的相对贡献，并进一步分析了f_fungi的关键影响因素。研究发现，岷江冷杉和糙皮桦通过菌丝途径获取的氮在总氮吸收中的贡献与根途径相当，菌丝途径对岷江冷杉总氮吸收的贡献范围为33.6% ~ 71.8%，对糙皮桦的贡献范围为41.6% ~ 63.0%。此外，土壤C/N、年平均温度及土壤pH是f_fungi的关键影响因素。本研究为深入理解自然生态系统中外生菌根树木氮获取策略及其影响因素提供了新的思路和见解。

关键词: 氮稳定同位素, 外生菌根树木, 菌丝途径, 氮吸收, 亚高山森林

Abstract: Ectomycorrhizal trees obtain nitrogen (N) through direct root uptake from soil (root pathway) and via ectomycorrhizal fungal mycelia translocated to roots (mycelia pathway). However, estimates of the contribution of the mycelial pathway to tree N acquisition (f_fungi) remain highly uncertain, and the key influencing factors are not well defined. In this study, we employed the ¹⁵N natural abundance method to quantify the relative contribution of the mycelial pathway to N acquisition in ectomycorrhizal trees (Abies fargesii var. faxoniana and Betula utilis) on the eastern Qinghai–Tibetan Plateau. We further analyzed the influencing factors of f_fungi. This study revealed that the contribution of mycelia pathways to total N uptake was comparable to that of root pathways in both A. fargesii var. faxoniana and B. utilis. Specifically, the mycelia pathway contributed to 33.6% ~ 71.8% of total N uptake in A. fargesii var. faxoniana and 41.6% ~ 63.0% in B. utilis. Moreover, the soil C/N, mean annual temperature and soil pH were identified as a key influencing factor for f_fungi. This study provides novel perspectives and insights for understanding N acquisition strategies and their influencing factors in ectomycorrhizal trees within natural ecosystems.

Key words: nitrogen stable isotope, ectomycorrhizal tree, mycelia pathway, nitrogen uptake, subalpine forest

陈淼, 陈健, 刘顺, 许格希, 冯秋红, 史作民. 外生菌根真菌对青藏高原东缘岷江冷杉和糙皮桦氮获取贡献及其影响因素. . DOI: 10.17521/cjpe.2025.0252

CHEN Miao, CHEN Jian, LIU Shun, XU Ge-xi, FENG Qiu-hong, SHI Zuo-min. The contribution and influencing factors of ectomycorrhizal fungi to nitrogen acquisition for Abies fargesii var. faxoniana and Betula utilis on the eastern Qinghai–Tibetan Plateau. Chinese Journal of Plant Ecology. DOI: 10.17521/cjpe.2025.0252

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