植物生态学报 ›› 2008, Vol. 32 ›› Issue (5): 994-1001.DOI: 10.3773/j.issn.1005-264x.2008.05.003
收稿日期:
2008-01-17
接受日期:
2008-04-27
出版日期:
2008-01-17
发布日期:
2008-09-30
通讯作者:
贺学礼
作者简介:
*(xuelh1256@yahoo.com.cn)基金资助:
HE Xue-Li(), ZHANG Huan-Shi, ZHAO Li-Li
Received:
2008-01-17
Accepted:
2008-04-27
Online:
2008-01-17
Published:
2008-09-30
Contact:
HE Xue-Li
摘要:
利用两种不同土壤研究了水分胁迫和接种AM真菌(摩西球囊霉(Glomus mosseae)和油蒿(Artemisia ordosica)根际土著AM真菌)对毛乌素沙地重要演替物种油蒿生长和抗旱性的影响。结果表明, 两种土壤中水分胁迫没有显著影响油蒿的植株形态和含水量, 但严重抑制了菌根侵染率。水分胁迫促使油蒿提高叶片保水能力, 抑制N、P在地上部的分配。在胁迫前期SOD活性较高, 而POD活性在后期较高。同一水分条件下接种AM真菌显著提高了AM真菌侵染率, 土壤中孢子数显著增多, 提高了植株分枝数并促进侧根发育, 显著提高根冠比和植株保水能力, 加强了根系对全磷、全氮的吸收。接种AM真菌的植株可溶性糖和丙二醛含量较低, 可溶性蛋白含量无显著变化, SOD和POD活性提高, 油蒿抗旱性加强。水分胁迫下在不同土壤中接种不同AM真菌对油蒿的促进效应差异较大, 接种土著AM真菌的效果优于摩西球囊霉单一接种。干旱导致菌根侵染率下降是宿主植物吸水能力下降的原因之一, 在植物生长前期接种AM真菌可以增强植物抵抗生长中后期环境干旱的能力。
贺学礼, 张焕仕, 赵丽莉. 不同土壤中水分胁迫和AM真菌对油蒿抗旱性的影响. 植物生态学报, 2008, 32(5): 994-1001. DOI: 10.3773/j.issn.1005-264x.2008.05.003
HE Xue-Li, ZHANG Huan-Shi, ZHAO Li-Li. EFFECTS OF AM FUNGI AND WATER STRESS ON DROUGHT RESISTANCE OF ARTEMISIA ORDOSICA IN DIFFERENT SOILS. Chinese Journal of Plant Ecology, 2008, 32(5): 994-1001. DOI: 10.3773/j.issn.1005-264x.2008.05.003
土壤来源 Soil origion | pH | 速效氮 Available N (μg?g-1) | 速效磷Available P (μg?g-1) | 速效钾Available K (μg?g-1) | 有机质 Organic Matter (g?kg-1) | 孢子数 Spore number (No.? 100g-1soil) | 田间持水量Field moisture capacity (%) |
---|---|---|---|---|---|---|---|
研究站Research station | 7.94 | 18.32 | 4.51 | 63.37 | 1.39 | 1 604 | 26.2 |
榆林 Yulin | 7.81 | 22.83 | 7.35 | 84.11 | 1.88 | 1 441 | 22.9 |
表1 试验用土的来源和基本理化性质
Table 1 Origion and basic physiochemical properties of soils used in the experiment
土壤来源 Soil origion | pH | 速效氮 Available N (μg?g-1) | 速效磷Available P (μg?g-1) | 速效钾Available K (μg?g-1) | 有机质 Organic Matter (g?kg-1) | 孢子数 Spore number (No.? 100g-1soil) | 田间持水量Field moisture capacity (%) |
---|---|---|---|---|---|---|---|
研究站Research station | 7.94 | 18.32 | 4.51 | 63.37 | 1.39 | 1 604 | 26.2 |
榆林 Yulin | 7.81 | 22.83 | 7.35 | 84.11 | 1.88 | 1 441 | 22.9 |
处理 Treatments | 分枝数 Branch number | 地上部干重 Shoot dry weight (g) | 主根长 Main root length (cm) | 主根干重Main root dry weight (g) | 侧根干重 Lateral root dry weight (g) | 根冠比Root/shoot ratio | 侵染率Infection rate (%) | 孢子数 Spore number | ||
---|---|---|---|---|---|---|---|---|---|---|
A | WW | IAMF | 16.2a | 1.34c | 14.03a | 0.380ab | 0.311d | 0.521b | 90.0a | 4 466.7a |
GM | 12.6b | 1.35c | 13.29a | 0.389ab | 0.448b | 0.635a | 70.0b | 2 361.5c | ||
CK | 11.7bc | 1.92a | 13.60a | 0.364c | 0.478a | 0.442c | 90.0a | 2 166.5d | ||
WS | IAMF | 12.3b | 1.57ab | 14.09a | 0.379ab | 0.448b | 0.530b | 73.3b | 3 250.0b | |
GM | 12.7b | 1.51b | 14.58a | 0.406a | 0.427bc | 0.558b | 66.7b | 2 506.7c | ||
CK | 10.7c | 1.33c | 13.72a | 0.328d | 0.414c | 0.572ab | 60.0c | 2 963.3c | ||
B | WW | IAMF | 11.6b | 1.70b | 12.61b | 0.428a | 0.452c | 0.645a | 53.3a | 4 526.5b |
GM | 14.2a | 2.08ab | 15.01a | 0.411b | 0.564a | 0.475c | 53.3a | 6 148.3ab | ||
CK | 12.9b | 2.50a | 15.12a | 0.398c | 0.538b | 0.528b | 46.7b | 903.5d | ||
WS | IAMF | 13.4ab | 1.96ab | 14.44a | 0.352d | 0.536b | 0.460c | 36.7c | 1 890.0c | |
GM | 13.1ab | 2.05ab | 15.00a | 0.354d | 0.412d | 0.378d | 33.3c | 9 400.0a | ||
CK | 14.0a | 2.09ab | 13.00b | 0.349d | 0.449c | 0.393d | 23.3d | 3 870.5b |
表2 不同土壤中AMF和水分胁迫对植株生长的影响
Table 2 Effects of AM fungi and water stress on the growth of Artenisia ordosica in different soils
处理 Treatments | 分枝数 Branch number | 地上部干重 Shoot dry weight (g) | 主根长 Main root length (cm) | 主根干重Main root dry weight (g) | 侧根干重 Lateral root dry weight (g) | 根冠比Root/shoot ratio | 侵染率Infection rate (%) | 孢子数 Spore number | ||
---|---|---|---|---|---|---|---|---|---|---|
A | WW | IAMF | 16.2a | 1.34c | 14.03a | 0.380ab | 0.311d | 0.521b | 90.0a | 4 466.7a |
GM | 12.6b | 1.35c | 13.29a | 0.389ab | 0.448b | 0.635a | 70.0b | 2 361.5c | ||
CK | 11.7bc | 1.92a | 13.60a | 0.364c | 0.478a | 0.442c | 90.0a | 2 166.5d | ||
WS | IAMF | 12.3b | 1.57ab | 14.09a | 0.379ab | 0.448b | 0.530b | 73.3b | 3 250.0b | |
GM | 12.7b | 1.51b | 14.58a | 0.406a | 0.427bc | 0.558b | 66.7b | 2 506.7c | ||
CK | 10.7c | 1.33c | 13.72a | 0.328d | 0.414c | 0.572ab | 60.0c | 2 963.3c | ||
B | WW | IAMF | 11.6b | 1.70b | 12.61b | 0.428a | 0.452c | 0.645a | 53.3a | 4 526.5b |
GM | 14.2a | 2.08ab | 15.01a | 0.411b | 0.564a | 0.475c | 53.3a | 6 148.3ab | ||
CK | 12.9b | 2.50a | 15.12a | 0.398c | 0.538b | 0.528b | 46.7b | 903.5d | ||
WS | IAMF | 13.4ab | 1.96ab | 14.44a | 0.352d | 0.536b | 0.460c | 36.7c | 1 890.0c | |
GM | 13.1ab | 2.05ab | 15.00a | 0.354d | 0.412d | 0.378d | 33.3c | 9 400.0a | ||
CK | 14.0a | 2.09ab | 13.00b | 0.349d | 0.449c | 0.393d | 23.3d | 3 870.5b |
处 理 Treatments | 地上部含水量 Water content of shoot (%) | 主根含水量 Water content of main root (%) | 侧根含水量 Water content of lateral root (%) | 地上部 Shoot | 根部 Root | ||||
---|---|---|---|---|---|---|---|---|---|
全P Total P (%) | 全N Total N (%) | 全P Total P (%) | 全N Total N (%) | ||||||
A | WW | IAMF | 55.26b | 59.99a | 69.13ab | 0.079a | 0.76d | 0.097b | 0.461b |
GM | 66.55a | 59.43a | 71.47a | 0.059b | 1.29b | 0.136a | 0.236c | ||
CK | 55.01b | 57.49b | 68.29c | 0.078a | 1.02c | 0.070c | 0.642a | ||
WS | IAMF | 54.28bc | 55.96c | 68.89bc | 0.058b | 1.16bc | 0.072c | 0.375bc | |
GM | 56.07b | 57.12b | 69.98ab | 0.064b | 1.01c | 0.065cd | 0.697a | ||
CK | 53.99c | 59.84a | 69.13ab | 0.062b | 1.56a | 0.060d | 0.549ab | ||
B | WW | IAMF | 58.59a | 57.50b | 68.21b | 0.088a | 1.83a | 0.095b | 0.86a |
GM | 56.14c | 66.44a | 69.06ab | 0.105a | 1.65a | 0.096b | 0.50b | ||
CK | 56.04c | 34.09d | 64.36d | 0.095a | 0.54d | 0.116a | 0.48b | ||
WS | IAMF | 57.32bc | 56.16b | 69.47a | 0.060b | 1.22b | 0.114a | 0.70a | |
GM | 58.28ab | 56.90b | 68.11bc | 0.076b | 0.97c | 0.115a | 0.41bc | ||
CK | 56.39bc | 49.14c | 67.03c | 0.075b | 0.34d | 0.098b | 0.32c |
表3 不同土壤中AMF和水分胁迫对植株水分、全P、全N含量的影响
Table 3 Effects of AM fungi and water stress on the water content and total P and total N contents of Artemisia ordosica in different soils
处 理 Treatments | 地上部含水量 Water content of shoot (%) | 主根含水量 Water content of main root (%) | 侧根含水量 Water content of lateral root (%) | 地上部 Shoot | 根部 Root | ||||
---|---|---|---|---|---|---|---|---|---|
全P Total P (%) | 全N Total N (%) | 全P Total P (%) | 全N Total N (%) | ||||||
A | WW | IAMF | 55.26b | 59.99a | 69.13ab | 0.079a | 0.76d | 0.097b | 0.461b |
GM | 66.55a | 59.43a | 71.47a | 0.059b | 1.29b | 0.136a | 0.236c | ||
CK | 55.01b | 57.49b | 68.29c | 0.078a | 1.02c | 0.070c | 0.642a | ||
WS | IAMF | 54.28bc | 55.96c | 68.89bc | 0.058b | 1.16bc | 0.072c | 0.375bc | |
GM | 56.07b | 57.12b | 69.98ab | 0.064b | 1.01c | 0.065cd | 0.697a | ||
CK | 53.99c | 59.84a | 69.13ab | 0.062b | 1.56a | 0.060d | 0.549ab | ||
B | WW | IAMF | 58.59a | 57.50b | 68.21b | 0.088a | 1.83a | 0.095b | 0.86a |
GM | 56.14c | 66.44a | 69.06ab | 0.105a | 1.65a | 0.096b | 0.50b | ||
CK | 56.04c | 34.09d | 64.36d | 0.095a | 0.54d | 0.116a | 0.48b | ||
WS | IAMF | 57.32bc | 56.16b | 69.47a | 0.060b | 1.22b | 0.114a | 0.70a | |
GM | 58.28ab | 56.90b | 68.11bc | 0.076b | 0.97c | 0.115a | 0.41bc | ||
CK | 56.39bc | 49.14c | 67.03c | 0.075b | 0.34d | 0.098b | 0.32c |
图1 不同土壤中AMF和水分胁迫对植株保水力和可溶性糖含量的影响 1: 前期 Early stage 2: 中期 Middle stage 3: 后期 Later stage 4: 末期 End stage IN: 接种土著 AMF Indigenous AM fungi inoculation GC: 接种摩西球囊霉 G. mosseae inoculation CK: 未接种 Non-inoculation A、B、WW、WS: 同表2 See Table 2
Fig. 1 Effects of AM fungi and water stress on the water retention capacity and the content of soluble sugar of Artemisia ordosica in different soils.
图2 不同土壤中AMF和水分胁迫对油蒿保护系统和丙二醛含量的影响 图例、图注同图1 Legends and notes see Fig. 1
Fig. 2 Effects of AM fungi and water stress on the protective system and the content of MDA of Artemisia ordosica in different soils
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