元江干热河谷毛枝青冈和三叶漆抗氧化系统季节变化

doi:10.3773/j.issn.1005-264x.2008.05.002

摘要/Abstract

摘要：

为探讨我国西南干热河谷这一严酷生境中植物抗氧化系统对多种胁迫因子的响应机制, 以该地区最干热的元江河谷萨王纳植被中光合能力有明显差异的两个优势种——常绿的毛枝青冈(Cyclobalanopsis helferiana)和干热季落叶的三叶漆(Terminthia paniculata)为材料, 研究了其抗氧化系统活性在高温雨季、干凉季和干热季的变化规律。结果表明: 从总体上看两树种抗氧化系统在干凉季活性最高, 然而, 两树种谷胱甘肽转移酶和谷胱甘肽过氧化物酶都在随后的干热季特异表达。两树种主要非酶抗氧化物质——抗坏血酸(ASC)和谷胱甘肽库容量与水-水循环起端酶超氧化物歧化酶(SOD)活性差异不大, 但光合速率低的三叶漆水-水循环和抗坏血酸-谷胱甘肽循环其它酶活性显著高于光合强的毛枝青冈。三叶漆抗氧化系统比毛枝青冈启动积极, 但后者有更持久的抗氧化能力。与其它逆境中植物相比, 两树种有更发达的抗氧化系统, 故能始终保持相对低的丙二醛含量。

关键词: 干旱, 冷害, 光氧化, 多重胁迫

Abstract:

Aims Hot-dry valleys in southwestern China are adverse habitats. The foliar antioxidant system of plants growing there must play an important role in protecting their photosynthetic apparatus against photo-oxidation. Our aim was to characterize the responses of the antioxidant system to seasonal changes of multiple abiotic stresses in two dominant tree species in the hot-dry valley of the Yuanjiang River, Yunnan.

Methods We chose two savanna species with different photosynthetic rates for study: the evergreen Cyclobalanopsis helferiana(oak) and drought-deciduous Terminthia paniculata(sumach). We examined changes of the two main antioxidants, ascorbate (ASC) and glutathione contents and the activities of all of the antioxidant enzymes of the water-water and ASC-glutathione cycle in these two species from the hot-rainy season to the chill-dry season and to the warm-dry season.

Important findings Both species showed the highest antioxidant activity in the chill-dry season; however, they had the highest activities of glutathione transferase and glutathione peroxidase in the subsequent warm-dry season. They had similar ASC and glutathione contents and activity of SOD, the initial enzyme of water-water cycle, but sumach displayed higher activities of most antioxidant enzymes in the water-water and ASC-glutathione cycles in all seasons compared to oak. Overall, sumach activated its antioxidant system more actively than oak, yet oak had more persistent antioxidant activity than sumach. Compared with activities of antioxidant systems of plants growing in other adverse conditions, the two study species have stronger antioxidant capacity, which is consistent with their absolutely lower contents of malondialdehyde in all seasons.

Key words: chilling, drought, multiple stresses, photo-oxidation

朱俊杰, 曹坤芳. 元江干热河谷毛枝青冈和三叶漆抗氧化系统季节变化. 植物生态学报, 2008, 32(5): 985-993. DOI: 10.3773/j.issn.1005-264x.2008.05.002

ZHU Jun-Jie, CAO Kun-Fang. SEASONAL CHANGES IN THE FOLIAR ANTIOXIDANT SYSTEMS IN CYCLOBALANOPSIS HELFERIANA AND TERMINTHIA PANICULATA IN THE HOT-DRY VALLEY OF THE YUANJIANG RIVER, CHINA. Chinese Journal of Plant Ecology, 2008, 32(5): 985-993. DOI: 10.3773/j.issn.1005-264x.2008.05.002

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2008.05.002

https://www.plant-ecology.com/CN/Y2008/V32/I5/985

图/表 5

表1 元江干热河谷2003年1月至2004年3月降水和气温变化特征

Table 1 Monthly rainfall and temperature during the study period from January 2003 to March 2004

月/年 Month/year	月降水 Monthly rainfall (mm)	月最低温 Monthly minimum temperature (℃)	月均温度 Monthly mean temperature (℃)	月最高温 Monthly maximum temperature (℃)
01/2003 02/2003 03/2003 04/2003 05/2003 06/2003 07/2003 08/2003 09/2003 10/2003 11/2003 12/2003 01/2004 02/2004 03/2004	67.8 5.9 37.1 10.0 66.8 141.6 136.4 121.8 48.8 36.6 0 11.8 19.4 7.6 5.4	6.5 9.6 12.1 16.3 20.2 22.1 23.3 22.8 18.3 17.8 11.1 9.2 6.4 6.7 11.1	16.1 19.1 22.5 27.3 28.9 28.2 29.3 28.4 26.5 25.6 21.1 19.0 16.6 19.1 23.5	30.0 33.9 35.7 39.5 40.1 37.5 40.2 38.3 36.9 37.3 32.8 29.7 30.4 35.4 38.7

图1 干热河谷毛枝青冈和三叶漆叶片相对含水量、光合速率、电子传递量子效率、丙二醛和超氧产生速率的季节变化图中数据为平均值(n=4), 柱上方不同字母表示同一树种不同季节间差异显著(p<0.05) Means significantly different within each species (n=4) were labeled by different letters (p<0.05)

Fig. 1 Seasonal changes of relative water content, CO2 assimilation rate, quantum yield of photosynthetic electron transport, malondialdehyde (MDA) contents and superoxide (O2-· ) radical production rate inCyclobalanopsis helferiana (oak) and Terminthia paniculata (sumach) in the hot-dry valley of Yuanjiang River

图2 毛枝青冈和三叶漆抗坏血酸(ASC)、脱氢抗坏血酸(dASC)和谷胱甘肽含量(GSSG, 氧化型谷胱甘肽; GSH, 还原型谷胱甘肽)及转换状况的季节变化图注同图1 Note see Fig. 1

Fig. 2 Seasonal changes of ASC, dASC, GSSG and GSH content, dASC/ASC and GSH/(GSH+GSSG) ratio in Cyclobalanopsis helferiana(oak) and Terminthia paniculata (sumach) in the hot-dry valley of Yuanjiang River

图3 毛枝青冈和三叶漆清除H2O2主要抗氧化酶的季节变化图注同图1 Note see Fig. 1

Fig. 3 Seasonal changes of SOD, APX, CAT, POX activities in Cyclobalanopsis helferiana(oak) and Terminthia paniculata (sumach) in the hot-dry valley of Yuanjiang River

图4 毛枝青冈和三叶漆抗坏血酸和谷胱甘肽代谢抗氧化酶的季节变化图注同图1 Note see Fig. 1

Fig. 4 Seasonal changes of DHAR, MDAR, GR, GP and GT activity in Cyclobalanopsis helferiana (oak) and Terminthia paniculata (sumach) in the hot-dry valley of Yuanjiang River

参考文献 26

[1]	Aebi H (1984). Catalase in vitro. Method in Enzymology, 105,121-126.
[2]	Aono M, Kubo A, Saji H, Natori T, Tanaka K, Kondo N (1991). Resistance to active oxygen toxicity of transgenic Nicotiana tabacum that expresses the gene for glutathione reductase from Escherichia coli. Plant and Cell Physiology, 32,691-697.
[3]	Asada K (1999). The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50,601-63. URL PMID
[4]	Bradford MM (1976). A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72,248-254. DOI URL PMID
[5]	Cao KF, Guo YH, Cai ZQ (2006). Photosynthesis and antioxidant enzyme activity in breadfruit, jackfruit and mangosteen in southern Yunnan, China. Journal of Horticultural Science & Biotechnology, 81,168-172.
[6]	Elstner EF, Heupel A (1976). Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Analytical Biochemistry, 70,616-620. DOI URL PMID
[7]	Foyer CH, Noctor G (2005). Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant, Cell and Environment, 28,1056-1071.
[8]	Giannopolitis CN, Ries SK (1977). Superoxide dismutase. Ⅰ.Occurrence in higher plants. Plant Physiology, 113,1193-1201.
[9]	Grace SC, Logan BA (1996). Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. Plant Physiology, 112,1631-1640. DOI URL PMID
[10]	Gupta AS, Webb RP, Holaday AS, Allen RD (1993). Overexpression of superoxide dismutase protects plants from oxidative stress (induction of ascorbate peroxidase in superoxide dismutase-overexpressing plants). Plant Physiology, 103,1067-1073. URL PMID
[11]	Hodges DM, DeLong JM, Forney CF, Prange RK (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207,604-611.
[12]	Huner NPA, Öquist G, Sarhan F (1998). Energy balance and acclimation to light and cold. Trends in Plant Science, 3,224-230.
[13]	Jin ZZ (金振洲), Ou XK (欧晓昆) (2000). Vegetations in the Hot and Dry Valleys along the Yuanjiang, Nujiang, Jinshajiang, and Lancangjiang Rivers(元江、怒江、金沙江、澜沧江干热河谷植被). Yunnan University Press, Kunming, 1-297. (in Chinese)
[14]	Kocsy G, Galiba G, Brunold C (2001). Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiologia Plantarum, 113,158-164. URL PMID
[15]	Light GG, Mahan JR, Roxas VP, Allen RD (2005). Transgenic cotton ( Gossypium hirsutum L.) seedlings expressing a tobacco glutathione s-transferase fail to provide improved stress tolerance. Planta, 222,346-354. DOI URL PMID
[16]	Li Y (李筠), Deng XP (邓西平), Kwak SS (郭尚洙), Tanaka K (田中净) (2006). Drought tolerance of transgenic sweet potato expressing both Cu/Zn superoxide dismutase and ascorbate peroxidase. Journal of Plant Physiology and Molecular Biology (植物生理与分子生物学报), 32,451-457. (in Chinese with English abstract)
[17]	Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999). Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiology, 119,1091-1099. DOI URL PMID
[18]	Del Carmen Córdoba-Pedregosa M, Córdoba F, Villalba JM, González-Reyes JA (2003). Zonal changes in ascorbate and hydrogen peroxide contents, peroxidase, and ascorbate-related enzyme activities in onion roots. Plant Physiology, 131,697-706. DOI URL PMID
[19]	McKersie BD, Leshem YY (1994). Stress and Stress Coping in Cultivated Plants. Kluwer Academic Publishers, Dordrecht, The Netherlands.
[20]	Müller-Moulé P, Patricia LC, Niyogi KK (2003). Ascorbate deficiency can limit violaxanthin de-epoxidase activity in vivo. Plant Physiology, 128,970-977. DOI URL PMID
[21]	Nakano Y, Asada K (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22,867-880.
[22]	Polle A, Otter T, Seifert F (1994). Apoplastic peroxidases and lignification in needles of norway spruce ( Picea abies L.). Plant Physiology, l06,53-60.
[23]	Rossel JB, Walter PB, Hendrickson L, Chow WS, Poole A, Mullineaux PM, Pogson BJ (2006). 2 gene expression reveals a link between responses to high light and drought tolerance. Plant, Cell and Environment, 29,269-281. URL PMID
[24]	Verhoeven AS, Annie S, Mai T, John W (2005). Seasonal changes in leaf antioxidant systems and xanthophyll cycle characteristics in Taxus×media growing in sun and shade environments. Physiologia Plantarum, 123,428-434.
[25]	Volk S, Feierabend J (1989). Photoinactivation of catalase at low temperature and its relevance to photosynthetic and peroxide metabolism in leaves. Plant, Cell and Environment, 12,701-712.
[26]	Yu SW (余叔文), Tang ZC (汤章城) (1998). Plant Physiology and Molecular Biology (植物生理与分子生物学). Science Press, Beijing, 366. (in Chinese)