盐胁迫对两种无芒雀麦种子萌发的影响

doi:10.17521/cjpe.2007.0064

摘要/Abstract

摘要：

在实验室中对两种无芒雀麦本地种(Bromus inermis cv. Xilinguole)和引进种(Bromus stamineus)种子在浓度为0.5%、1.0%、1.5%的Na₂SO₄、NaCl和MgCl₂ 3种单盐胁迫下进行发芽实验,结果表明:与对照相比,在以上3种盐胁迫下,随着胁迫浓度的增加,两种无芒雀麦种子的发芽率、发芽指数和发芽值均有不同程度的降低,相对盐害率升高,种子开始发芽的时间推迟且其发芽过程延长;在3种盐中,NaCl的胁迫对两种植物种子萌发的抑制作用最大,其中浓度为1.0% NaCl的胁迫对引进种和本地种无芒雀麦发芽率的抑制分别达到了68.67%和14%,在浓度为1.5%的NaCl胁迫下两种供试植物的发芽率均为零;通过建立不同盐分处理与两种无芒雀麦种子发芽率之间的回归方程得出,引进无芒雀麦种子芽期的耐盐性较本地无芒雀麦强。

关键词: 盐胁迫, 萌发, 无芒雀麦, 种子

Abstract:

Aims Use of saline land has become a major agricultural issue around the world. China has 27 millions kilometer saline land, and the area is increasing. Our objective was to compare the germination ability of the saline-tolerant Bromus stamineus from the USA and B. inermis cv. Xilinguole under saline conditions.
Methods We applied Na₂SO₄, NaCl and MgCl₂ at concentrations of 0.5%, 1.0% and 1.5% for salt stress treatments and recorded germination percentage, germination energy, germination index, germination value etc. We put seeds of the two plants in culture dishes covered with a filter paper and a glass bell jar with a small hole. Each culture dish was put on a beaker containing different salt solution, with gauze connecting the solution and the filter paper. The gauze extended outside the bell jar to evaporate and avoid salt accumulation on the filter paper, keeping the germination environment constant.
Important findings Germination percentage, germination index and germination value for both species declined with increased salinity, with values reduced more for B. inermis cv. Xilinguole than for B. stamineus. Times for seeds to germinate and complete their germination were prolonged under high salinity stress. NaCl had greater effects than Na₂SO₄ and MgCl₂ on both species. Overall, B. stamineus exhibited greater germination ability with salt stress than B. inermis cv. Xilinguole.

Key words: salt stress, germination, Bromus stamineus, Bromus inermis cv. Xilinguole, seed

胡生荣, 高永, 武飞, 米志英, 张雨. 盐胁迫对两种无芒雀麦种子萌发的影响. 植物生态学报, 2007, 31(3): 513-520. DOI: 10.17521/cjpe.2007.0064

HU Sheng-Rong, GAO Yong, WU Fei, MI Zhi-Ying, ZHANG Yu. EFFECT OF SALT STRESS ON GERMINATION OF BROMUS STAMINEUS AND BROMUS INERMIS CV. XILINGUOLE. Chinese Journal of Plant Ecology, 2007, 31(3): 513-520. DOI: 10.17521/cjpe.2007.0064

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2007.0064

https://www.plant-ecology.com/CN/Y2007/V31/I3/513

图/表 10

表1 不同发芽环境下本地种种子7 d的累积发芽率(%)

Table 1 Accumulating germination percentage (%) of Bromus inermis cv. Xilinguole seeds in 7 days

发芽环境 Germination environment	发芽天数 Germination days (d)
发芽环境 Germination environment	1	2	3	4	5	6	7
CK	75.33	80.00	81.33	82.67	82.67	82.67	82.67
A1	25.33	36.00	43.33	47.33	50.67	52.67	52.67
A2	12.67	25.33	27.33	32.00	36.67	38.67	39.33
A3	3.33	3.33	5.33	11.33	17.33	20.00	22.00
B1	5.33	22.67	28.67	38.00	47.33	50.00	50.67
B2	2.00	6.00	8.00	9.33	12.00	14.00	16.00
B3	0.00	0.00	0.00	0.00	0.00	0.00	0.00
C1	10.00	20.00	40.00	46.00	51.33	52.00	54.67
C2	3.33	19.33	24.67	30.67	36.00	36.00	36.00
C3	5.33	10.00	19.33	25.33	29.33	34.67	34.67

表2 不同发芽环境下引进种种子7 d的累积发芽率(%)

Table 2 Accumulating germination percentage (%) of Bromus stamineus seeds in 7 days

发芽环境 Germination environment	发芽天数 Germination days (d)
发芽环境 Germination environment	1	2	3	4	5	6	7
CK	22.67	26.67	28.00	28.67	28.67	28.67	28.67
A1	11.33	18.00	22.67	25.33	25.33	25.33	25.33
A2	8.67	15.33	18.00	18.67	19.33	21.33	21.33
A3	6.00	6.00	13.33	15.33	18.67	18.67	18.67
B1	5.33	6.00	7.33	13.33	18.00	19.33	21.33
B2	0.00	1.33	4.00	6.00	10.67	14.00	16.67
B3	0.00	0.00	0.00	0.00	0.00	0.00	0.00
C1	12.67	12.67	14.00	16.67	18.00	19.34	22.00
C2	6.66	12.67	12.67	14.67	14.67	15.34	15.34
C3	0.00	6.67	12.67	12.67	13.34	14.00	14.00

图1 盐胁迫对两种植物种子平均发芽天数的影响

Fig.1 Effect of salt stress on MLIT of two plants CK、A1、A2、A3、B1、B2、B3、C1、C2、C3:同表1 See Table 1

图2 盐胁迫对两种植物种子相对发芽势的影响

Fig.2 Effect of salt stress on relative germination vigor (RGe) of two plants CK、A1、A2、A3、B1、B2、B3、C1、C2、C3:同表1 See Table 1

图3 盐胁迫对两种植物种子相对发芽指数的影响

Fig.3 Effect of salt stress on relative germination index (RGi) of two plants CK、A1、A2、A3、B1、B2、B3、C1、C2、C3:同表1 See Table 1

图4 盐胁迫对两种植物种子相对发芽值的影响

Fig.4 Effect of salt stress on relative germination value (RGV) of two plants CK、A1、A2、A3、B1、B2、B3、C1、C2、C3:同表1 See Table 1

图5 盐胁迫对两种植物种子相对盐害率的影响

Fig.5 Effect of salt stress on relative rate of harm by salt of two plants CK、A1、A2、A3、B1、B2、B3、C1、C2、C3:同表1 See Table 1

图6 盐胁迫下发芽率下降百分比变化趋势图

Fig.6 Effect of salt stress on decrease rate of germination percentage (Gp) of two plants CK、A1、A2、A3、B1、B2、B3、C1、C2、C3:同表1 See Table 1

表3 两种无芒雀麦种子在盐胁迫下相对发芽率的变化

Table 3 The change of relative germination percentage (RGp) of two plants under salt stress

植物种 Species	CK	A1	A2	A3	B1	B2	B3	C1	C2	C3
本地种 Bromus inermis cv. Xilinguole	100.00	66.80	48.84	25.61	62.63	18.15	0.00	65.14	40.93	40.26
引进种 B. stamineus	100.00	93.99	81.82	68.81	76.21	62.07	0.00	77.80	69.90	51.76

表4 不同盐处理与两种无芒雀麦种子发芽率之间的回归方程

Table 4 Linear regression between germination percentage (Gp) of two plants and salt stress

植物种 Species	盐分 Salt	回归方程 Linear regression	调整后R² Adjusted R square	显著性水平 Significance
本地种 Bromus inermis cv. Xilinguole	Na₂SO₄	Y=78.47-19.535X	0.953	0.016
	NaCl	Y=79.737-28.268X	0.968	0.011
	MgCl₂	Y=76.403-16.267X	0.819	0.062
引进种 B. stamineus	Na₂SO₄	Y=28.6-3.4X	0.992	0.003
	NaCl	Y=30.268-9.067X	0.890	0.037
	MgCl₂	Y=27.603-5.067X	0.907	0.032

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