温度变化对油菜及其伴生杂草种苗生长和幼苗生理特性的影响

doi:10.3724/SP.J.1258.2013.00116

摘要/Abstract

摘要：

以目前大面积栽培的甘蓝型油菜(Brassica napus) ‘秦优10号’和其田间常见的8种杂草播娘蒿(Descurainia sophia)、泽漆(Euphorbia helioscopia)、野燕麦(Avena fatua)、棒头草(Polypogon fugax)、婆婆纳(Veronica didyma)、看麦娘(Alopecurus aequalis)、灰绿藜(Chenopodium glaucum)、反枝苋(Amaranthus retroflexus)为试验材料, 利用人工气候培养箱控温, 研究了10 ℃/5 ℃ (昼/夜)、18 ℃/10 ℃、25 ℃/20 ℃、35 ℃/30 ℃、40 ℃/35 ℃不同温度幅度处理对它们的发芽势、发芽率、幼苗形态及生理指标的影响, 旨在为预测气候变化环境下油菜田恶性靶标杂草的演替趋势提供参考依据。试验结果表明: 不同供试杂草对温度变化表现出明显的适应性差异, 看麦娘、灰绿藜和反枝苋的最适萌发温度偏高, 如反枝苋在高温处理(40 ℃/35 ℃)下种子的发芽率达到最高(91%); 而播娘蒿、泽漆、野燕麦、棒头草、婆婆纳种子的最适萌发温度主要集中于10-18 ℃范围内。进一步测试研究表明, 高温处理(40 ℃/35 ℃)下, 反枝苋幼苗生长旺盛, 体内丙二醛的积累也明显低于低温(10 ℃/5 ℃)处理, 可溶性糖和可溶性蛋白含量以及保护酶活性也较高, 表明反枝苋对高温胁迫有较好的适应性, 灰绿藜对高温的响应与反枝苋相似, 在高温气候年份, 要严防这2种草害加重; 而在低温处理(10 ℃/5 ℃)下, 野燕麦的种子萌发率达到100%, 其幼苗生长旺盛, 体内可溶性糖和可溶性蛋白含量及保护酶活性较高, 播娘蒿对低温胁迫的响应类似于野燕麦, 因此在寒冷年份需加强对野燕麦和播娘蒿草害的预防。供试油菜品种相对于所有供试杂草对温度变化的适应性更好, 其种子在不同温度处理下均保持较稳定的高发芽率, 且幼苗长势良好, 表明‘秦优10号’是一个对温度变化适应性很好的油菜品种。

关键词: 适应性, 油菜, 幼苗生长, 温度变化, 杂草

Abstract:

Aims Weed communities in rape fields have changed with occurrence of extreme weather events and global warming. Our objective was to investigate the effects of different regimes of temperature change on rape (Brassica napus ‘Qinyou-10’) and eight companion weeds, i.e. Descurainia sophia, Euphorbia helioscopia, Avena fatua, Polypogon fugax, Veronica didyma, Alopecurus aequalis, Chenopodium glaucum and Amaranthus retroflexus, to help with predicting the succession of companion weed communities under extreme temperatures.
Methods Seed germination, seedling growth and several physiological properties were investigated under five temperature treatments (10 °C/5 °C, 18 °C /10 °C, 25 °C /20 °C, 35 °C /30 °C and 40 °C /35 °C) (day/night) controlled by a climate-control growth chamber. Physiological properties tested included contents of malondialdehyde (MDA), soluble sugar and soluble protein, and activities of superoxide dismutase (SOD) and peroxidase (POD).
Important findings There were significant differences among the weed species in response to temperature change. The optimal temperatures for seed germination in Alopecurus aequalis, Chenopodium glaucum and Amaranthus retroflexus were high; for instance, the highest seed germination rate (91%) occurred at temperature of 40 °C /35 °C in Amaranthus retroflexus. In contrast, seed germination in Descurainia sophia, Euphorbia helioscopia, Avena fatua, Polypogon fugax and Veronica didyma required a much lower optimal temperature (i.e. 10-18 °C). At the highest treatment temperature (40 °C /35 °C), Amaranthus retroflexus seedlings grew vigorously, and their MDA contents were much lower than under low temperature treatment of 10 °C /5 °C. Moreover, the concentrations of soluble sugar and soluble protein and the activities of SOD and POD in Amaranthus retroflexus seedlings all increased with temperature, indicating the strong ability of this weed species in adapting to high temperature stress. Chenopodium glaucum had the similar responses as Amaranthus retroflexus in those properties to changes in temperature. Therefore, these two weeds should be paid more attention with the increasingly intensified global warming. Contrarily, at the lowest treatment temperature of 10 °C/5 °C, seed germination reached 100%, and the seedlings exhibited high soluble sugar and soluble protein concentrations as well as high SOD and POD activities in Avena fatua. Descurainia sophia had the similar responses as Avena fatua. Therefore, we should take additional measures to prevent malignant growth of Avena fatua and Descurainia sophia in cold years. Rape (‘Qinyou-10’) had stable and high seed germination rate, and grew well as seedlings, indicating that it better adapted to temperature changes compared to the eight companion weeds. We deduced ‘Qinyou-10’ is a rape species that can adapt well to temperature changes.

Key words: adaptability, rape, seedling growth, temperature change, weed

张凯, 慕小倩, 孙晓玉, 汪梦竹, 胡胜武. 温度变化对油菜及其伴生杂草种苗生长和幼苗生理特性的影响. 植物生态学报, 2013, 37(12): 1132-1141. DOI: 10.3724/SP.J.1258.2013.00116

ZHANG Kai, MU Xiao-Qian, SUN Xiao-Yu, WANG Meng-Zhu, HU Sheng-Wu. Effects of temperature change on seed germination, seedling growth and physiological characteristics in rape and companion weeds. Chinese Journal of Plant Ecology, 2013, 37(12): 1132-1141. DOI: 10.3724/SP.J.1258.2013.00116

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2013.00116

https://www.plant-ecology.com/CN/Y2013/V37/I12/1132

图/表 4

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	发芽势 Germination energy (%)					发芽率 Germination rate (%)
	T1	T2	T3	T4	T5	T1	T2	T3	T4	T5
播娘蒿 Descurainia sophia	42^b	80^a	11^c	10^c	10^c	52^b	92^a	20^c	16^d	14^d
泽漆 Euphorbia helioscopia	20^b	31^a	36^a	20^b	16^c	35^a	40^a	40^a	35^a	22^b
野燕麦 Avena fatua	80^a	90^a	90^a	10^b	5^c	100^a	100^a	100^a	20^b	12^c
棒头草 Polypogon fugax	16^e	70^a	62^b	40^d	56^c	32^c	87^b	90^a	91^a	90^a
婆婆纳 Veronica didyma	32^c	87^a	60^b	65^b	60^b	62^d	100^a	95^b	90^c	91^c
看麦娘 Alopecurus aequalis	2^c	3^b	6^a	3^b	6^a	3^d	21^c	25^b	26^b	35^a
灰绿藜 Chenopodium glaucum	5^c	4^c	3^c	10^b	19^a	6^e	10^d	12^c	15^b	26^a
反枝苋 Amaranthus retroflexus	2^e	4^d	16^c	60^b	85^a	3^e	7^d	20^c	85^b	91^a
油菜 Brassica napus	81^c	90^b	96^a	84^c	62^d	88^b	98^a	100^a	98^a	100^a

	发芽势 Germination energy (%)					发芽率 Germination rate (%)
	T1	T2	T3	T4	T5	T1	T2	T3	T4	T5
播娘蒿 Descurainia sophia	42^b	80^a	11^c	10^c	10^c	52^b	92^a	20^c	16^d	14^d
泽漆 Euphorbia helioscopia	20^b	31^a	36^a	20^b	16^c	35^a	40^a	40^a	35^a	22^b
野燕麦 Avena fatua	80^a	90^a	90^a	10^b	5^c	100^a	100^a	100^a	20^b	12^c
棒头草 Polypogon fugax	16^e	70^a	62^b	40^d	56^c	32^c	87^b	90^a	91^a	90^a
婆婆纳 Veronica didyma	32^c	87^a	60^b	65^b	60^b	62^d	100^a	95^b	90^c	91^c
看麦娘 Alopecurus aequalis	2^c	3^b	6^a	3^b	6^a	3^d	21^c	25^b	26^b	35^a
灰绿藜 Chenopodium glaucum	5^c	4^c	3^c	10^b	19^a	6^e	10^d	12^c	15^b	26^a
反枝苋 Amaranthus retroflexus	2^e	4^d	16^c	60^b	85^a	3^e	7^d	20^c	85^b	91^a
油菜 Brassica napus	81^c	90^b	96^a	84^c	62^d	88^b	98^a	100^a	98^a	100^a

		播娘蒿 Descurainia sophia	泽漆 Euphorbia helioscopia	野燕麦 Avena fatua	棒头草 Polypogon fugax	婆婆纳 Veronica didyma	看麦娘 Alopecurus aequalis	灰绿藜 Chenopodium glaucum	反枝苋 Amaranthus retroflexus
丙二醛 Malonaldehyde (nmol·g^-1 FW)	T1	4.98 ± 0.64^ab	3.41 ± 0.14^b	1.31 ± 0.22^a	5.29 ± 0.95^a	5.21 ± 1.02^b	46.31 ± 1.80^a	1.75 ± 0.09^a	10.18 ± 0.63^a
	T2	2.80 ± 0.09^c	2.42 ± 0.08^b	0.97 ± 0.45^b	2.35 ± 0.24^b	4.13 ± 1.42^b	10.61 ± 0.80^bc	1.45 ± 0.12^ab	8.44 ± 0.52^b
	T3	3.73 ± 0.15^bc	0.36 ± 0.01^c	0.27 ± 0.22^c	1.63 ± 0.05^b	5.12 ± 0.31^b	8.80 ± 1.45^c	1.27 ± 0.02^b	5.59 ± 0.01^c
	T4	4.05 ± 0.96^abc	2.36 ± 0.25^b	0.30 ± 0.49^c	1.75 ± 0.22^b	6.58 ± 0.31^b	15.35 ± 0.43^bc	0.73 ± 0.05^c	4.69 ± 0.13^c
	T5	5.56 ± 0.12^a	6.10 ± 0.68^a	0.35 ± 0.02^c	4.62 ± 0.07^a	9.53 ± 1.47^a	23.80 ± 0.15^b	1.51 ± 0.20^ab	4.97 ± 0.14^c
可溶性糖 Soluble sugar (mg·g^-1 FW)	T1	55.67 ± 0.31^a	152.89 ± 0.50^b	70.47 ± 0.22^a	80.70 ± 0.09^a	299.47 ± 0.02^ab	511.55 ± 0.24^d	42.35 ± 0.02^b	82.93 ± 0.39^a
	T2	50.05 ± 0.37^ab	117.56 ± 0.42^c	57.23 ± 0.45^b	70.30 ± 0.44^b	201.60 ± 1.14^c	621.08 ± 3.12^b	39.37 ± 0.42^a	68.71 ± 0.32^b
	T3	39.57 ± 0.61^b	60.42 ± 0.03^d	29.87 ± 0.05^c	36.76 ± 0.01^d	223.91 ± 0.05^c	562.93 ± 0.20^c	10.20 ± 0.05^b	53.94 ± 0.29^c
	T4	42.91 ± 0.20^ab	105.60 ± 0.59^c	33.45 ± 0.02^c	61.27 ± 0.24^e	253.65 ± 3.71^bc	737.33 ± 2.06^a	34.73 ± 0.31^a	72.30 ± 0.22^b
	T5	54.48 ± 1.16^ab	241.21 ± 1.53^a	34.50 ± 0.02^c	102.50 ± 0.05^c	329.000 ± 0.03^a	398.82 ± 0.44^e	10.21 ± 0.02^b	76.72 ± 0.24^ab
可溶性蛋白 Soluble protein (mg·g^-1FW)	T1	9.66 ± 0.14^e	12.96 ± 0.14^c	15.62 ± 0.21^b	20.10 ± 0.33^b	4.56 ± 0.15^b	33.42 ± 0.87^b	11.00 ± 0.03^c	21.01 ± 0.75^b
	T2	16.64 ± 0.11^b	13.92 ± 0.12^b	16.64 ± 0.19^a	17.37 ± 0.08^c	4.91 ± 0.07^b	9.99 ± 0.20^d	17.16 ± 0.13^a	19.75 ± 0.04^c
	T3	11.63 ± 0.07^d	10.58 ± 0.19^d	9.60 ± 0.14^e	10.60 ± 0.20^e	3.35 ± 0.26^c	9.69 ± 0.42^d	6.73 ± 0.10^d	16.78 ± 0.23^d
	T4	20.40 ± 0.22^a	16.16 ± 0.08^a	10.92 ± 0.16^d	11.62 ± 0.11^d	3.28 ± 0.38^c	29.52 ± 0.55^c	16.63 ± 0.15^b	21.87 ± 0.14^b
	T5	12.18 ± 0.07^c	9.63 ± 0.20^e	14.25 ± 0.08^c	28.00 ± 0.11^a	13.97 ± 0.14^a	50.76 ± 0.73^a	11.17 ± 0.05^c	25.68 ± 0.25^a
超氧化物歧化酶 Superoxide dismutase U·g^-1 FW·min^-1	T1	258.70 ± 3.39^b	323.77 ± 6.89^b	130.75 ± 2.33^a	121.15 ± 6.48^a	421.88 ± 1.04^b	317.95 ± 6.22^b	456.37 ± 1.08^b	199.20 ± 10.14^b
	T2	126.04 ± 1.39^d	184.54 ± 0.75^c	65.62 ± 1.06^c	92.58 ± 1.97^bc	94.76 ± 0.52^e	119.82 ± 9.50^d	206.69 ± 8.65^d	59.31 ± 3.41^c
	T3	50.47 ± 1.94^e	51.33 ± 11.12^d	29.65 ± 4.11^d	68.51 ± 2.43^d	131.03 ± 6.13^d	104.27 ± 3.15^d	63.08 ± 1.84^e	78.84 ± 3.97^c
	T4	228.43 ± 6.22^c	171.35 ± 1.50^c	70.50 ± 10.35^c	80.02 ± 10.69^cd	220.27 ± 11.01^c	143.24 ± 2.45^c	374.39 ± 3.17^c	199.87 ± 3.68^b
	T5	337.23 ± 1.91^a	352.63 ± 1.92^a	91.20 ± 2.62^b	101.77 ± 2.35^b	463.63 ± 8.03^a	500.75 ± 6.98^a	472.60 ± 1.10^a	551.15 ± 6.23^a
过氧化物酶 Peroxidase U·g^-1 FW·min^-1	T1	348.37 ± 5.52^c	451.48 ± 1.52^c	1203.67 ± 18.18^a	450.99 ± 4.52^b	487.79 ± 2.56^a	63.53 ± 6.64^c	147.79 ± 0.54^b	182.59 ± 6.75^b
	T2	709.55 ± 2.76^b	346.97 ± 4.33^d	979.87 ± 2.61^b	341.28 ± 8.36^c	70.59 ± 1.81^d	55.88 ± 1.86^c	70.76 ± 1.48^e	219.972 ± 6.52^ab
	T3	356.38 ± 1.40^c	327.98 ± 0.32^e	633.91 ± 13.43^e	253.00 ± 9.35^c	106.68 ± 1.88^c	57.03 ± 4.47^c	81.17 ± 0.28^d	173.28 ± 1.90^b
	T4	851.68 ± 1.82^a	745.16 ± 9.70^b	748.65 ± 16.32^d	723.16 ± 2.12^a	293.68 ± 6.08^b	86.47 ± 4.99^b	161.50 ± 2.40^a	192.78 ± 1.79^b
	T5	257.59 ± 2.21^d	831.68 ± 2.87^a	805.43 ± 6.62^d	775.99 ± 5.13^a	514.87 ± 2.05^a	118.44 ± 1.29^a	118.28 ± 1.61^c	266.70 ± 6.90^a

		播娘蒿 Descurainia sophia	泽漆 Euphorbia helioscopia	野燕麦 Avena fatua	棒头草 Polypogon fugax	婆婆纳 Veronica didyma	看麦娘 Alopecurus aequalis	灰绿藜 Chenopodium glaucum	反枝苋 Amaranthus retroflexus
丙二醛 Malonaldehyde (nmol·g^-1 FW)	T1	4.98 ± 0.64^ab	3.41 ± 0.14^b	1.31 ± 0.22^a	5.29 ± 0.95^a	5.21 ± 1.02^b	46.31 ± 1.80^a	1.75 ± 0.09^a	10.18 ± 0.63^a
	T2	2.80 ± 0.09^c	2.42 ± 0.08^b	0.97 ± 0.45^b	2.35 ± 0.24^b	4.13 ± 1.42^b	10.61 ± 0.80^bc	1.45 ± 0.12^ab	8.44 ± 0.52^b
	T3	3.73 ± 0.15^bc	0.36 ± 0.01^c	0.27 ± 0.22^c	1.63 ± 0.05^b	5.12 ± 0.31^b	8.80 ± 1.45^c	1.27 ± 0.02^b	5.59 ± 0.01^c
	T4	4.05 ± 0.96^abc	2.36 ± 0.25^b	0.30 ± 0.49^c	1.75 ± 0.22^b	6.58 ± 0.31^b	15.35 ± 0.43^bc	0.73 ± 0.05^c	4.69 ± 0.13^c
	T5	5.56 ± 0.12^a	6.10 ± 0.68^a	0.35 ± 0.02^c	4.62 ± 0.07^a	9.53 ± 1.47^a	23.80 ± 0.15^b	1.51 ± 0.20^ab	4.97 ± 0.14^c
可溶性糖 Soluble sugar (mg·g^-1 FW)	T1	55.67 ± 0.31^a	152.89 ± 0.50^b	70.47 ± 0.22^a	80.70 ± 0.09^a	299.47 ± 0.02^ab	511.55 ± 0.24^d	42.35 ± 0.02^b	82.93 ± 0.39^a
	T2	50.05 ± 0.37^ab	117.56 ± 0.42^c	57.23 ± 0.45^b	70.30 ± 0.44^b	201.60 ± 1.14^c	621.08 ± 3.12^b	39.37 ± 0.42^a	68.71 ± 0.32^b
	T3	39.57 ± 0.61^b	60.42 ± 0.03^d	29.87 ± 0.05^c	36.76 ± 0.01^d	223.91 ± 0.05^c	562.93 ± 0.20^c	10.20 ± 0.05^b	53.94 ± 0.29^c
	T4	42.91 ± 0.20^ab	105.60 ± 0.59^c	33.45 ± 0.02^c	61.27 ± 0.24^e	253.65 ± 3.71^bc	737.33 ± 2.06^a	34.73 ± 0.31^a	72.30 ± 0.22^b
	T5	54.48 ± 1.16^ab	241.21 ± 1.53^a	34.50 ± 0.02^c	102.50 ± 0.05^c	329.000 ± 0.03^a	398.82 ± 0.44^e	10.21 ± 0.02^b	76.72 ± 0.24^ab
可溶性蛋白 Soluble protein (mg·g^-1FW)	T1	9.66 ± 0.14^e	12.96 ± 0.14^c	15.62 ± 0.21^b	20.10 ± 0.33^b	4.56 ± 0.15^b	33.42 ± 0.87^b	11.00 ± 0.03^c	21.01 ± 0.75^b
	T2	16.64 ± 0.11^b	13.92 ± 0.12^b	16.64 ± 0.19^a	17.37 ± 0.08^c	4.91 ± 0.07^b	9.99 ± 0.20^d	17.16 ± 0.13^a	19.75 ± 0.04^c
	T3	11.63 ± 0.07^d	10.58 ± 0.19^d	9.60 ± 0.14^e	10.60 ± 0.20^e	3.35 ± 0.26^c	9.69 ± 0.42^d	6.73 ± 0.10^d	16.78 ± 0.23^d
	T4	20.40 ± 0.22^a	16.16 ± 0.08^a	10.92 ± 0.16^d	11.62 ± 0.11^d	3.28 ± 0.38^c	29.52 ± 0.55^c	16.63 ± 0.15^b	21.87 ± 0.14^b
	T5	12.18 ± 0.07^c	9.63 ± 0.20^e	14.25 ± 0.08^c	28.00 ± 0.11^a	13.97 ± 0.14^a	50.76 ± 0.73^a	11.17 ± 0.05^c	25.68 ± 0.25^a
超氧化物歧化酶 Superoxide dismutase U·g^-1 FW·min^-1	T1	258.70 ± 3.39^b	323.77 ± 6.89^b	130.75 ± 2.33^a	121.15 ± 6.48^a	421.88 ± 1.04^b	317.95 ± 6.22^b	456.37 ± 1.08^b	199.20 ± 10.14^b
	T2	126.04 ± 1.39^d	184.54 ± 0.75^c	65.62 ± 1.06^c	92.58 ± 1.97^bc	94.76 ± 0.52^e	119.82 ± 9.50^d	206.69 ± 8.65^d	59.31 ± 3.41^c
	T3	50.47 ± 1.94^e	51.33 ± 11.12^d	29.65 ± 4.11^d	68.51 ± 2.43^d	131.03 ± 6.13^d	104.27 ± 3.15^d	63.08 ± 1.84^e	78.84 ± 3.97^c
	T4	228.43 ± 6.22^c	171.35 ± 1.50^c	70.50 ± 10.35^c	80.02 ± 10.69^cd	220.27 ± 11.01^c	143.24 ± 2.45^c	374.39 ± 3.17^c	199.87 ± 3.68^b
	T5	337.23 ± 1.91^a	352.63 ± 1.92^a	91.20 ± 2.62^b	101.77 ± 2.35^b	463.63 ± 8.03^a	500.75 ± 6.98^a	472.60 ± 1.10^a	551.15 ± 6.23^a
过氧化物酶 Peroxidase U·g^-1 FW·min^-1	T1	348.37 ± 5.52^c	451.48 ± 1.52^c	1203.67 ± 18.18^a	450.99 ± 4.52^b	487.79 ± 2.56^a	63.53 ± 6.64^c	147.79 ± 0.54^b	182.59 ± 6.75^b
	T2	709.55 ± 2.76^b	346.97 ± 4.33^d	979.87 ± 2.61^b	341.28 ± 8.36^c	70.59 ± 1.81^d	55.88 ± 1.86^c	70.76 ± 1.48^e	219.972 ± 6.52^ab
	T3	356.38 ± 1.40^c	327.98 ± 0.32^e	633.91 ± 13.43^e	253.00 ± 9.35^c	106.68 ± 1.88^c	57.03 ± 4.47^c	81.17 ± 0.28^d	173.28 ± 1.90^b
	T4	851.68 ± 1.82^a	745.16 ± 9.70^b	748.65 ± 16.32^d	723.16 ± 2.12^a	293.68 ± 6.08^b	86.47 ± 4.99^b	161.50 ± 2.40^a	192.78 ± 1.79^b
	T5	257.59 ± 2.21^d	831.68 ± 2.87^a	805.43 ± 6.62^d	775.99 ± 5.13^a	514.87 ± 2.05^a	118.44 ± 1.29^a	118.28 ± 1.61^c	266.70 ± 6.90^a