三峡库区岸生植物秋华柳对水淹的光合和生长响应

doi:10.17521/cjpe.2007.0115

摘要/Abstract

摘要：

为阐明三峡库区岸生植物秋华柳(Salix variegata)对水淹的耐受机制,模拟三峡库区消落带水淹发生的情况,研究了在不同水淹时间和水淹深度处理下秋华柳的光合和生长特性。实验设置了对照(不进行水淹,常规供水管理)、水淹根部(植株置于水中,植株地下部分被淹没)、水下0.5 m(植株置于水中,植株顶部在水面下0.5 m)和水下2 m(植株置于水中,植株顶部在水面下2 m)4个不同的水淹深度和0、10、20、40、60和90 d 6个不同的水淹时间处理,并测定了在不同水淹时间和水淹深度处理下秋华柳的光合作用、叶绿素荧光和生长。研究结果发现:随着水淹时间的延长,对照和水淹根部植株都具有高的净光合速率、表观量子效率和羧化效率。水淹40 d后,相同水淹深度处理秋华柳植株的净光合速率显著高于耐水湿环境的垂柳(Salix babylonica)(p<0.05)。水淹90 d后,全淹处理植株的光合能力较对照有显著的下降(p<0.05),对照、水下0.5 m和水下2 m植株的净光合速率分别为13.2、10.1和8.05 μmol·m^-2·s^-1,同时全淹植株PSII的最大光化学效率也有一定程度的下降,显著低于对照和水淹根部处理的植株(p<0.05)。水淹40、60和90 d后,全淹植株的胞间CO₂浓度都高于对照和水淹根部植株。随着水淹时间的增加,水淹根部植株不定根数量不断增加,而全淹植株只有极少量的不定根产生。水淹根部植株的主茎长的增量、分枝数的增量、主茎新生叶片数、根生物量的积累和总生物量的积累都高于全淹植株,全淹植株在水淹过程中,其主茎长、分枝数、主茎新叶数、根生物量和总生物量都有增加,同时其凋落叶片较多。水淹90 d后,秋华柳植株的存活率为100%。研究结果表明,秋华柳在经过较长时间的水淹后,表现出较强的光合和生长适应性,可以考虑将秋华柳列为三峡库区消落带植被构建的物种之一。

关键词: 三峡库区, 秋华柳, 水淹, 光合响应, 生长

Abstract:

Aims The aim was to reveal the effects of flooding on photosynthesis and growth of the riparian plant Salix variegata for revegetation of riparian areas in Three Gorges Reservoir Region.

Methods Four flooding treatments were applied to the plants: no flooding, belowground submergence and whole plant submergence to water depths of 0.5 and 2 m. Net photosynthetic rate, apparent quantum yield, carboxylation efficiency, intercellular CO₂ concentration and maximal photochemical efficiency of PSII (F_v/F_m) were determined at 0, 40, 60 and 90 d. The numbers of adventitious roots were determined at 6, 15, 25 and 32 d. Increase of stem length, increase of number of shoots, number of newly grown leaves on stem, number of shed leaves on stem, increase of root biomass and increase of plant biomass were determined at 10, 20, 40, 60 and 90 d.

Important findings Control and belowground-submerged plants maintained high net photosynthetic rate, apparent quantum yield and carboxylation efficiency during inundation. At 40 d, the net photosynthetic rate of S. variegata was significantly higher than that in the waterlogging-tolerant species S. babylonica (p<0.05). At 60 and 90 d, the photosynthetic capacity andF_v/F_m of wholly submerged plants decreased significantly as compared with that of the control and belowground-submerged plants (p<0.05), but the plants could still maintain high photosynthetic capacity. At 40, 60 and 90 d, the intercellular CO₂ concentration of wholly submerged plants was higher than that of the control and belowground-submerged plants. At 32 d, many adventitious roots were developed in belowground-submerged plants, while the wholly submerged plants had few adventitious roots. The belowground-submerged plants had greater increase in stem length, number of shoots, and newly generated leaves on stem, root biomass and total plant biomass than wholly submerged plants. During the flooding period, the stem length, number of shoots, and newly generated leaves on stem, root biomass and total plant biomass of wholly submerged plants increased, and the number of shed leaves of wholly submerged plants was higher than that of the control and belowground-submerged plants. At 90 d, all belowground-submerged and wholly submerged S. variegata were alive. Therefore, S. variegata has high photosynthetic capacity and growth adaptability over inundation of 90 d and is a promising species for revegetation of the riparian zone in the Three Gorges Reservoir Region.

Key words: Three Gorges Reservoir Region, Salix variegata, flooding, photosynthetic response, growth

罗芳丽, 曾波, 陈婷, 叶小齐, 刘巅. 三峡库区岸生植物秋华柳对水淹的光合和生长响应. 植物生态学报, 2007, 31(5): 910-918. DOI: 10.17521/cjpe.2007.0115

LUO Fang-Li, ZENG Bo, CHEN Ting, YE Xiao-Qi, LIU Dian. RESPONSE TO SIMULATED FLOODING OF PHOTOSYNTHESIS AND GROWTH OF RIPARIAN PLANT SALIX VARIEGATA IN THE THREE GORGES RESERVOIR REGION OF CHINA. Chinese Journal of Plant Ecology, 2007, 31(5): 910-918. DOI: 10.17521/cjpe.2007.0115

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2007.0115

https://www.plant-ecology.com/CN/Y2007/V31/I5/910

图/表 4

图1 不同水淹条件下秋华柳的净光合速率、表观量子效率、羧化效率、胞间CO2浓度和PSⅡ的最大光化学效率(平均值±标准误) T1~T4同表1 See Table 1 对每一水淹时间水平,标有不同字母的各处理之间有显著差异(p=0.05) For each level of submergence duration of treatments, means of treatments with different letters are significantly different (p=0.05)

Fig.1 Net photosynthetic rate (Pn), apparent quantum yield (α), carboxylation efficiency (g'm), intercellular CO2 concentration (Ci) and maximal photochemical efficiency of PSⅡ (Fv/Fm) (mean±SE) of Salix variegata subjected to different levels and durations of water submergence stress

表1 不同水淹时间和水淹深度下秋华柳的不定根数量

Table 1 Adventitious roots number of Salix variegata subjected to different levels and durations of water submergence stress

水淹时间 Duration of flooding(d)	水淹深度 Underwater depth
水淹时间 Duration of flooding(d)	T1	T2	T3	T4
6	0	3	0	0
15	0	17	0	0
25	0	20	1	0
32	0	24	4	2

图2 不同水淹条件下秋华柳主茎长、分枝、新长叶片数和凋落叶片数的增加量(平均值±标准误) 图注同图1 Notes see Fig. 1

Fig.2 Increase of stem length, number of shoots, number of newly generated leaves on stem and number of shed leaves on stem (mean±SE) in Salix variegata subjected to different levels and durations of water submergence stress

表2 水淹40 d后秋华柳和垂柳的净光合速率(平均值±标准误)

Table 2 The photosynthetic rate (μmol·m-2·s-1) (mean±SE) of Salix variegata and S. babylonica after 40 d inundation treatments

	对照 Control	水淹根部 Belowground submergence	水下2 m Submergence with 2 m water depth
秋华柳 Salix variegata	19.9±1.65^a	18.1±2.03^a	14.2±1.82^a
垂柳S. babylonica	17.5±1.33^a	11.5±1.25^b	9.13±1.06^b

参考文献 31

[1]	Berry JA, Downton WJS (1982). Environmental regulation of photosynthesis. In: Govind Jed. Photosynthesis Vol Ⅱ. Academic Press, New York.
[2]	Institute of Botany, the Chinese Academy of Sciences(中国科学院植物研究所) (2001). Iconographia Cormophytorum Sinicorum. Tomus. (中国高等植物图鉴)(第一册). Science Press, Beijing, 362. (in Chinese)
[3]	Chen HJ, Qualls RG, Blank RR (2005). Effect of soil flooding on photosynthesis, carbohydrate partitioning and nutrient uptake in the invasive exotic Lepidium latifolium. Aquatic Botany, 82,250-268.
[4]	Chen HJ, Qualls RG, Miller GC (2002). Adaptive responses of Lepidium latifolium to soil flooding: biomass allocation, adventitious rooting, aerenchyma formation and ethylene production. Environmental and Experimental Botany, 48,119-128.
[5]	Farquhar GD, Sharkey TD (1982). Stomatal conductance and photosynthesis. Annual Review Plant Physiology, 33,317-345.
[6]	Gravatt DA, Kirby CJ (1998). Patterns of photosynthesis and starch allocation in seedlings of four bottomland hardwood tree species subjected to flooding. Tree Physiology, 18,411-417. DOI URL PMID
[7]	Groeneveld HW, Voesenek LACJ (2003). Submergence-induced petiole elongation in Rumex palustris is controlled by developmental stage and storage compounds. Plant and Soil, 253,115-123.
[8]	Hook DD (1984). Waterlogging tolerance of lowland tree species of south. Southern Journal of Applied Forestry, 8,136-149.
[9]	Kawase M, Whitmoger RE (1980). Aerenchyma development in waterlogged plants. American Journal of Botany, 67,18-28.
[10]	Kozlowski TT (1997). Responses of woody plants to flooding and salinity. Tree Physiology, 1,1-29. DOI URL PMID
[11]	Kozlowski TT, Pallardy SG (1984). Effect of flooding on water, carbohydrate and mineral relations. In: Kozlowski TTed. Flooding and Plant Growth. Academic Press Inc., London,165-193.
[12]	Krause GH, Weis E (1991). Chlorophyll fluorescence and photosynthesis: the basics. Annual Review of Plant Physiology and Plant Molecular Biology, 42,313-349.
[13]	Laan P, Blom CWPM (1990). Growth and survival responses of Rumex species to flooded and submerged conditions: the importance of shootelongation, underwater photosynthesis and reserve carbohydrates. Journal of Experimental Botany, 41,775-783.
[14]	Li DH (李敦海), Liu YD (刘永定), Song LR (宋立荣) (1999). The effect of salt stress on some physiological and biochemical characteristics of Nostoc sphaeroides Kütz (cyanobacterium). Acta Hydrobiologica Sinica (水生生物学报), 23,414-419. (in Chinese with English abstract)
[15]	Maxwell K, Johnson GN (2000). Chlorophyll fluorescence-a practical guide. Journal of Experimental Botany, 51,659-668. DOI URL PMID
[16]	Mielke MS, de Almeida AF, Gomes FP, Aguilar MAG, Mangabeira PAO (2003). Leaf gas exchange, chlorophyll fluorescence and growth responses of Genipa americana seedlings to soil flooding. Environment and Experimental Botany, 50,221-231.
[17]	Mommer L, Lenssen JPM, Huber H, Visser EJW, Kroon HD (2006). Ecophysiological determinants of plant performance under flooding: a comparative study of seven plant families. Journal of Ecology, 94,1117-1129.
[18]	Mommer L, Visser EJW (2005). Underwater photosynthesis in flooded terrestrial plants: a matter of leaf plasticity. Annals of Botany, 96,581-589. DOI URL PMID
[19]	Naidoo G, Naidoo S (1992). Waterlogging responses of Sporobolus virginicus(L.). Kunth. Oecologia, 90,445-450. DOI URL PMID
[20]	Pezeshki SR (1994). Plant response to flooding. In: Wilkinson REed. Plant-Environment Interactions. Marcel Dekker, New York,289-321.
[21]	Pezeshki SR, Pardue JH, Delaune RD (1996). Leaf gas exchange and growth of flood-tolerant and flood-sensitive tree species under low soil redox conditions. Tree Physiology, 16,453-458. DOI URL PMID
[22]	Pezeshki SR (2001). Wetland plant responses to soil flooding. Environmental and Experimental Botany, 46,299-312.
[23]	Rowe RN, Beardsell DV (1973). Waterlogging of fruit trees. Horticultural Abstracts, 43,533-548.
[24]	Scott HD, Angule JD (1989). Flooding duration effects on soybean growth and yield. Agronomy Journal, 81,631-636.
[25]	Vervuren PJA, Beurskens SMJH, Blom CWPM (1999). Light acclimation, CO ₂ response and long-term capacity of underwater photosynthesis in three terrestrial plant species. Plant, Cell and Environment, 22,959-968.
[26]	Visser EJW, Voesenek LACJ, Vartapetian BB (2003). Flooding and plant growth. Annals of Botany, 91,107-109.
[27]	Wang WQ (王文泉), Zhang FS (张福锁) (2001). The physiological and molecular mechanism of adaptation to anaerobiosis in higher plants. Plant Physiology Communications (植物生理学通讯), 2,63-70. (in Chinese)
[28]	Wang WQ (王文泉), Zheng YZ (郑永战), Mei HX (梅鸿献), Zhang FS (张福锁) (2003). Physiological and structure adaptation in roots of genotypes with different tolerance to waterlogging in Sesame ( Sesamum indicum L.) under anoxia stress. Journal of Plant Genetic Resources (植物遗传资源学报), 4,214-219. (in Chinese with English abstract)
[29]	Wei HP (魏和平), Li RQ (利容千) (2000). Effect of flooding on morphology, structure and ATPase activity in adventitious root apical cells of maize seedlings. Acta Phytoecologica Sinica (植物生态学报), 24,293-297. (in Chinese with English abstract)
[30]	Yordanova RY, Alexieva VS, Popova LP (2003). Influence of root oxygen deficiency on photosynthesis and antioxidant status in barley plants. Russian Journal of Plant Physiology, 50,163-167.
[31]	Yuan H (袁辉), Wang LA (王里奥), Zhan YH (詹艳慧), Huang C (黄川), Hu G (胡刚) (2006). Health evaluation system of the water-level-fluctuation zone in the Three Gorges area. Resources and Environment in the Yangtze Basin (长江流域资源与环境), 15,249-253. (in Chinese with English abstract)