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丛枝菌根真菌与不同功能细菌联用对紫花苜蓿的促生作用探究

姜庆宏, 丁露, 王哲, 郑春丽, 冯昭绰   

  1. 内蒙古科技大学能源与环境学院, 内蒙古包头 014010, 内蒙古自治区 014010 中国
    上海第二工业大学资源与环境工程学院, 上海 201209, 上海 201209 中国
  • 收稿日期:2025-10-27 修回日期:2026-03-04
  • 基金资助:
    内蒙古自然科学基金(2023MS04010); 国家自然科学基金项目(52264013); 内蒙古自治区直属高校基本科研业务费专项资金资助(2023CXPT004)

Combination of Arbuscular Mycorrhizal Fungi and Functional Bacteria on the Growth Promotion of Medicago sativa in Semi-Arid Coal Mining Areas

Qing-hong Jiang, Lu Ding, Zhe Wang, Chun-li Zheng, Zhao-chuo Feng   

  1. , Inner Mongolia University of Science and Technology, School of Energy and Environment, Baotou, Inner Mongolia 014010, China 014010, China
    , Shanghai Polytechnic University, School of Resource and Environmental Engineering, Shanghai 201209, China 201209, China
  • Received:2025-10-27 Revised:2026-03-04
  • Supported by:
    the Inner Mongolia Natural Science Foundation(2023MS04010); The National Natural Science Foundation of China(52264013); Special Funds for Basic Scientific Research Business Expenses of Universities Directly under the Inner Mongolia Autonomous Region(2023CXPT004)

摘要： 为解决鄂尔多斯半干旱煤矿区土壤养分贫瘠, 植株定植困难等问题, 本研究围绕丛枝菌根真菌与不同功能细菌多元组合对紫花苜蓿根系进行侵染, 构建了作用周期短且稳定性强的土壤微生物修复体系, 探究了其对鄂尔多斯煤矿半干旱土壤理化性质的改良以及对该地区植株的促生效应。结果表明, ARB(AMF+根瘤菌+巨大芽孢杆菌)、ARBK(AMF+根瘤菌+巨大芽孢杆菌+胶质芽孢杆菌)微生物体系在真菌与细菌的联合作用下对土壤理化性质的改善及紫花苜蓿的促生的效果较好。ARB组的优势菌属包括Kaistobacter菌属、节杆菌属(Arthrobacter)、黄色土源菌属(Flavisolibacter)等。与CK2相比，ARB体系使得紫花苜蓿根系土壤中的有机质、全氮、速效磷含量分别提高了93.7%、117.6%、215.6%; 土壤EEG和TG分别提升约195.2%、63.2%; 紫花苜蓿根系的AMF侵染率提高了76.87%, 增加了53.2条一级新根和8cm侧根长, 紫花苜蓿地上生物量提升了168.0%。ARBK体系下叶片营养含量和抗逆性的提升效果最佳, 叶绿素a、叶绿素b、胡萝卜素、可溶性蛋白含量分别提升约80.8%、77.8%、201.7%、90.5%, 可溶性糖含量和脯氨酸含量分别提升70.6%、66.9%。研究为解决半干旱矿区土壤贫瘠、植物定植困难等问题提供了科学参考。

关键词: 丛枝菌根真菌, 细菌真菌联用, 植物生长促进, 土壤微生物修复, 紫花苜蓿

Abstract: Aims Our objective was to overcome soil nutrient deficiency and vegetation establishment barriers. Methods This study developed a rapid-cycle, stable microbial remediation system by combining AMF with multifunctional bacteria. The system was applied to improve soil physicochemical properties and promote the growth of alfalfa (Medicago sativa) in Ordos coal mining soils. Important findings Experimental data revealed that the ARB (AMF + Rhizobium + Bacillus megaterium) and ARBK (AMF + Rhizobium + Bacillus megaterium + Bacillus mucilaginosus) microbial system substantially enhanced soil fertility and plant growth. The dominant bacterial genus in the ARB group included Kaistobacter, Arthrobacter, and Flavisolibacter. Compared with CK2 group, the ARB treatment elevated rhizosphere organic matter, total nitrogen, and available phosphorus by 93.7%, 117.6% and 215.6%, while increasing soil EEG (easily extractable glomalin) and TG (total glomalin) by 195.2% and 63.2%. Concurrently, the AMF infection rate in Alfalfa roots surged by 76.87%, accompanied by 53.2 primary new roots formation and 8cm extension of lateral roots, and 168.0% biomass accumulation in aboveground. The ARBK treatment exhibited superior leaf nutritional content and stress resistance, with chlorophyll a, chlorophyll b, carotenoids, and soluble protein rising by 80.8%, 77.8%, 201.7%, and 90.5%, respectively. Stress-resistant metabolites including soluble sugars and proline also improved by 70.6% and 66.9%. These findings provide a scientific basis for ecological restoration in soil nutrient-poor, difficult plant establishing semi-arid mining regions.

Key words: Arbuscular mycorrhizal fungi, bacterial-fungal combination, soil microbial remediation, plant growth promotion, Medicago sativa

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