植物生态学报

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亚热带森林不同菌根真菌树种叶片和细根的C、N、P化学计量特征

王梦雪1,胡明艳1,储诚进1,陈阳2,罗文启1,马子龙3   

  1. 1. 中山大学生态学院
    2. 中山大学生命科学学院
    3. 中山大学
  • 收稿日期:2024-10-21 修回日期:2025-01-17 发布日期:2025-01-20
  • 通讯作者: 马子龙

C, N, P stoichiometric characteristics of leaves and fine roots in different mycorrhizal tree species in subtropical forests

Meng-Xue WANG1,Ming-Yan HU2,Cheng-Jin CHU3,Yang CHEN3,Wen-Qi LUO4,Zilong Ma5   

  1. 1. 中山大学生态学院
    2. School of Ecology, Sun Yat-sen University,
    3. School of Life Sciences, Sun Yat-sen University
    4. School of Ecology, Sun Yat-sen University
    5. Sun Yat-sen University
  • Received:2024-10-21 Revised:2025-01-17
  • Contact: Zilong Ma

摘要: 【目的】菌根真菌与宿主植物的碳(C)、氮(N)、磷(P)及C、N、P化学计量特征紧密相关。然而,亚热带森林中不同菌根真菌树种叶片和细根的C、N、P化学计量特征是否具有显著相关性尚不清楚。因此,本研究旨在阐明丛枝菌根(AM)和外生菌根(ECM)树种叶片和细根的C、N、P化学计量特征有何差异以及AM和ECM树种叶片和细根的C、N、P化学计量比是否协同变化。【方法】本研究依托广东省黑石顶自然保护区森林动态监测样地,采取81个AM树种和16个ECM树种的叶片和细根样品并测定其C、N、P浓度。通过计算Blomberg's K统计量以检验系统发育信号,利用线性混合模型检验不同菌根类型树种之间叶片和细根的C、N、P浓度和化学计量比的差异,利用皮尔逊相关系数检验不同菌根类型树种叶片和细根中C、N、P浓度之间以及化学计量比之间的相关性,最后利用标准化主轴回归分析不同菌根类型树种叶片和细根之间C、N、P浓度及化学计量比的关系。【主要结果】AM树种细根的P浓度高于ECM树种,但是C:P低于ECM树种。AM和ECM树种叶片和细根的N浓度和P浓度之间,C:N和C:P之间均具有显著正相关性。AM树种叶片和细根的C、N、P浓度及化学计量比之间都存在显著相关性,而ECM树种叶片和细根N浓度、C:N、N:P之间存在显著相关性。本研究结果表明菌根真菌定殖会影响植物的化学元素组成及化学计量比,但是AM和ECM树种叶片和细根间的C、N、P化学计量比都具有显著相关性。

关键词: 丛枝菌根真菌, 外生菌根真菌, 化学计量比, 叶片, 细根

Abstract: Aims Mycorrhizal fungi are closely related to the host's carbon (C), nitrogen (N), phosphorus (P), and C, N, P stoichiometric characteristics. However, whether the C, N, P stoichiometric characteristics in leaves and fine roots of different mycorrhizal tree species are significantly correlated in subtropical forests remains unclear. Therefore, this study aims to clarify the differences of the C, N, P stoichiometric characteristics in leaves and fine roots be-tween arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) tree species, and whether the C, N, P stoichio-metric ratio in leaves and fine roots of AM and ECM tree species changes consistently. Methods This study collected leaf and fine root samples of 81 AM tree species and 16 ECM tree species from the forest dynamic monitoring sample plot in Heishiding Nature Reserve, located in Guangdong Province, and meas-ured their C, N, and P concentrations. We calculated Blomberg's K statistic to test phylogenetic signals, used the linear mixed model to test the differences in C, N and P concentrations and stoichiometric ratios of leaves and fine roots among different mycorrhizal species, and used the Pearson correlation coefficient to test the correlation be-tween C, N and P concentrations and stoichiometric ratios in leaves and fine roots of different mycorrhizal species. Finally, we used standardised major axis regression to analyze the relationship of C, N and P concentrations and stoichiometric ratios between leaves and fine roots in different mycorrhizal species. Important findings The P concentration in fine roots of AM species was higher than that of ECM species, but C:P was lower than that of ECM species. There was a significant positive correlation between the N and P concentra-tions, C:N and C:P in the leaves and fine roots of AM and ECM tree species. There was a significant correlation between the concentrations of C, N and P and stoichiometric ratios of leaves and fine roots in AM species, while there was a significant correlation between N concentration, C:N and N:P of leaves and fine roots in ECM species. The results of this study confirm that the colonization of mycorrhizal fungi affected the chemical element compo-sition and stoichiometric ratio of plants, but the C, N and P stoichiometric ratios between leaves and fine roots in AM and ECM tree species were all significantly correlated.

Key words: arbuscular mycorrhizal fungi, ectomycorrhizal fungi, stoichiometric ratio, leaves, fine roots