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丛枝菌根真菌与凋落物互作有助于加拿大一枝黄花应对营养胁迫

任曦彤, 李颖, 张雨, 熊胡安赫, 张蕊科, 祁珊珊, 戴志聪, 杜道林   

  1. 江苏大学环境与安全工程学院,镇江 212013Jiangsu University, 江苏 212013 中国
    江苏大学农业工程学部,镇江212013Jiangsu University, 江苏 212013 中国
    江苏大学农业工程学院,镇江212013, 江苏 212013 中国
    苏州科技大学,江苏水处理技术与材料协同创新中心,苏州215009, 江苏 215009 中国
    江苏大学京江学院,镇江 212028, 江苏 212013 中国
  • 收稿日期:2025-05-15 修回日期:2025-06-25
  • 基金资助:
    国家自然科学基金(32271587); 国家自然科学基金(32171509); 国家级大学生创新训练计划项目; 江苏大学大学生科研立项(23A118); 江苏大学“青年英才培育计划”

Interaction between Arbuscular Mycorrhizal Fungi and Litter contributes to the responses of Solidago canadensis to Nutrient Stress

REN Xitong, LI Ying, ZHANG Yu, XIONG Huanhe, ZHANG Ruike, QI Shanshan, DAI Zhicong, DU Daolin   

  1. Jiangsu University, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China 212013, China
    Jiangsu University, Faculty of Agricultural Engineering of Jiangsu University, Zhenjiang 212013, China 212013, China
    , School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China 212013, China
    , Suzhou University of Science and Technology, Jiangsu Collaborative Innovation Center of Technology and Material for Water Treatment, Suzhou 215009, China 215009, China
    Jiangsu University, Jingjiang College, Jiangsu University, Zhenjiang 212028, China 212013, China
  • Received:2025-05-15 Revised:2025-06-25
  • Supported by:
    Supported by the National Natural Science Foundation of China(32271587); Supported by the National Natural Science Foundation of China(32171509); National College Student Innovation Training Program; Student Research Project of Jiangsu University(23A118); Youth Talent Cultivation Program of Jiangsu University

摘要: 【目的】植物入侵往往引起本地物种多样性丧失,造成巨大的经济损失。用于解释外来植物成功入侵的“共生促进假说”提出入侵植物往往可以与具有促进作用的共生微生物形成良好的共生关系。为探究在不同营养条件下丛枝菌根真菌(AMF)及凋落物对入侵植物生长的贡献。【方法】本研究以恶性入侵植物加拿大一枝黄花(Solidago canadensis L.)为代表,通过接种幼套球囊霉(Glomus etunicatum)AMF菌剂并添加其叶片凋落物,探讨AMF和凋落物的互作在加拿大一枝黄花响应不同营养条件中的作用。【主要结果】添加凋落物显著提高加拿大一枝黄花的菌根侵染率及侵染丰度;在低营养情况下,AMF及凋落物都能显著促进植株的茎长、叶面积及地上生物量,但显著减少植株的根冠比,且AMF与凋落物互作的促进效果最为显著;在正常营养下,仅AMF与凋落物互作对植物的部分指标有显著影响——抑制了根的发育但提高了地上生物量。相对相互作用强度(RII)分析表明,AMF和凋落物的添加都对加拿大一枝黄花地上部分的生长有显著促进作用,且在低营养条件下的促进作用显著强于正常营养条件。研究结果表明,在低营养胁迫下加拿大一枝黄花可以通过AMF与凋落物的互作调整地上地下资源分配策略以应对低营养胁迫。研究结果有助于从共生微生物AMF与凋落物互作的视角解释外来入侵植物如何通过地上地下部分的资源分配策略响应不同营养胁迫,加深我们对入侵植物成功入侵的机制理解。

关键词: 植物入侵, 丛枝菌根真菌, 凋落物, 营养胁迫, 植物-微生物互作

Abstract: Aims Plant invasion often leads to the loss of local species diversity and causes significant economic losses. The Enhanced Mutualisms Hypothesis used to explain that some alien species have shown a remarkable ability to capitalize on novel but strong soil mutualists, which enhance their invasion success. In this study, the contributions of arbuscular mycorrhizal fungi (AMF) and litter to the growth of invasive plant under different nutrient conditions was investigated. Methods We inoculated AMF, Glomus etunicatum and leaf litter to explore the roles of AMF-litter interactions in the responses of notorious invasive Solidago canadensis to low nutrient stress. Important findings The results showed that litter addition significantly increased the mycorrhizal colonization rate and ratio of abundance class in S. canadensis. Under low-nutrient conditions, both AMF and litter promoted the stem length, leaf area and above-ground biomass, respectively, but significantly decreased the root-shoot ratio, and with the most pronounced synergistic enhancement observed in AMF-litter interactions. Under normal nutrient conditions, only AMF-litter interactions significantly inhibited the development of root system but increased the above-ground biomass in S. canadensis. Relative Interaction Intensity (RII) analysis revealed that AMF and litter significantly stimulated above-ground growth, with stronger effects under low-nutrient conditions compared to normal nutrient levels. These findings suggest that S. canadensis could adjust the allocation strategies for aboveground-belowground resource by AMF-litter interactions to respond low-nutrient stress. This study provides novel insights into how invasive plant utilize symbiotic AMF and litter interactions to mediate resource allocation under nutrient heterogeneity, advancing our understanding of the mechanism of successful invasion of alien plants.

Key words: Plant invasion, Arbuscular mycorrhizal fungi (AMF), Litter, Nutrient stress, Plant-microbe interaction