MONITORING LEAF CARBON AND NITROGEN STATUS IN WHEAT WITH LEAF COLOR CHARACTERISTICS

doi:10.17521/cjpe.2005.0060

Abstract

Abstract:

Non-destructive monitoring of crop carbon, nitrogen and carbon to nitrogen ratios (C/N) is important for precision fertilizer management. This research was conducted to determine the relationships of leaf carbon, leaf nitrogen, and leaf C/N ratios to post-heading leaf color (SPAD values) in wheat (Triticum aestivum) under different nitrogen and water supply levels. The results showed that leaf soluble sugar content were positively exponentially related to leaf color in the top three leaves, but there were no significant relationships between color differences or ratios among different leaf positions. Similar trends were found for leaf nitrogen status. The correlation sequence was: L 1>L 2>L 3 (correlation coefficients were 0.92, 0.86 and 0.71 for leaf nitrogen content and 0.84, 0.77 and 0.49 for leaf soluble sugar content, respectively). The best leaf position for monitoring leaf nitrogen and carbon status was the first leaf from the top in wheat (LSSC= 4.080 8 e 0.026 9SPADL 1, R 2=707 5; LNC=0.697 2e 0.026 1SPADL 1, R 2=837 6), with a RMSE of 0.22 and 0.04, respectively, for model testing. In addition, leaf carbon to nitrogen ratios were not significantly related to leaf colors in different leaf positions but were linearly significantly related with color difference between L 1 and L 3 in wheat (C/N=0.071 3× (SPADL 1-SPADL 3) +4.459), with a RMSE of 0.07 for model testing. These results indicate that the leaf color of L 1 and color differences between L 1 and L 3 are good indicators of leaf soluble sugar content, nitrogen content and C/N in wheat leaves.

Key words: Wheat, Leaf color characteristics, Leaf position, Leaf sugar, Leaf nitrogen, Carbon to nitrogen ratios

TIAN Yong-Chao, ZHU Yan, CAO Wei-Xing. MONITORING LEAF CARBON AND NITROGEN STATUS IN WHEAT WITH LEAF COLOR CHARACTERISTICS[J]. Chin J Plant Ecol, 2005, 29(3): 457-460.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2005.0060

https://www.plant-ecology.com/EN/Y2005/V29/I3/457

Figures/Tables 4

Table 1 The coefficients of correlation between leaf color characters and leaf nitrogen content and soluble sugar content in wheat (R)

	叶色特征 Leaf color character
	L₁	L₂	L₃	L₁/L₂	L₁/L₃	L₂/L₃	\|L₁-L₂\|	\|L₁-L₃\|	\|L₂-L₃\|
叶片氮含量Leaf nitrogen content	0.92	0.86	0.71	0.27	0.25	0.24	0.00	0.23	0.29
叶片可溶性糖含量 Leaf soluble sugar content	0.84	0.77	0.49	0.28	0.20	0.12	0.01	0.34	0.42

Fig.1 Relationships of canopy leaf soluble sugar contents (A), nitrogen contents (B) to leaf SPAD values in wheat

Fig.2 Relationship between color difference of L1 and L3 and ratio of canopy leaf carbon to nitrogen in wheat

Fig.3 Comparisons of measured with predicted canopy leaf soluble sugar content, nitrogen content and C/N from the leaf color characters in wheat

References 12

[1]	Bronson KF, Chua TT, Booker JD, Keeling JW, Lascano RJ (2003). In-seasonnitrogenstatussensinginirrigatedcotton:II.Leafnitrogenandbiomass. SoilScienceSocietyofAmericaJour nal, 67,1439-1448.
[2]	Chen F (陈防), Lu JW (鲁剑巍) (1996). PreliminarystudyonrapiddiagnosisofcropnutrientstatususingtheSPAD-502chlorophyllmeter. HubeiAgriculturalSciences (湖北农业科学), 2,31-34. (inChinese).
[3]	Jin XC (金先春), Xu WS (徐威生), Zhang L (张玲), Qin F (秦峰), Huang SR (黄淑荣) (1994). Influenceofphysiologi calcharacteristicsatgrainfillingstageondyinggreenofwheatplantsindifferentvarieties. ActaAgronomicaSinica (作物学报), 20,99-105. (inChinesewithEnglishabstract).
[4]	Peng S, Garcia FV, Laza RC, Gassman KG (1993). Adjustmentforspecificleafweightimproveschlorophyllmeter'sestimateofriceleafnitrogenconcentration. AgronomyJournal, 83,926-928.
[5]	Shen ZQ (沈掌泉), Wang K (王珂), Zhu JY (朱君艳) (2002). Preliminarystudyondiagnosisofthenitrogenstatusoftworicevarietiesusingthechlorophyllmeter. BulletinofScienceandTechnology (科技通报), 18 (3),173-176. (inChinesewithEnglishabstract).
[6]	Singh B, Singh Y, Ladha SK, Bronson KF, Balasubramanian V, Singh J, Khind CS (2002). Chlorophyllmeter-andleafcolorchart-basednitrogenmanagementforriceandwheatinnorthwest ernIndia. AgronomyJournal, 94,821-829.
[7]	Turner CJ, Jund MF (1991). Chlorophyllmetertopredictnitrogentopdressrequirementforsemidwarfrice. AgronomyJournal, 83,926-928.
[8]	Wang SH (王绍华), Liu SH (刘胜环), Wang QS (王强盛), Ding YF (丁艳锋), Huang PS (黄丕生), Ling QH (凌启鸿) (2002a). Relationshipbetweenyieldformationandleafnitrogencontentandcolorinriceplant. JournalofNanjingAgriculturalUniversity (南京农业大学学报), 25 (4),1-5. (inChinesewithEnglishabstract).
[9]	Wang SH (王绍华), Cao WX (曹卫星), Wang QS (王强盛), Ding YF (丁艳锋), Huang PS (黄丕生), Ling QH (凌启鸿) (2002b). Positionaldistributionofleafcoloranddiagnosisofni trogennutritioninriceplant. ScientiaAgriculturaSinica (中国农业科学), 35,1461-1466. (inChinesewithEnglishab stract).
[10]	Wu LH (吴良欢), Tao QN (陶勤南) (1999). Nitrogenfertilizerapplicationbasedonthediagnosisofnitrogennutritionofriceplants. JournalofZhejiangAgriculturalUniversity (浙江农业大学学报) 25,135-138. (inChinesewithEnglishabstract).
[11]	Yang WH, Peng SB, Huang J, Sanico AL, Buresh RJ, Witt C (2003). Usingleafcolorchartstoestimateleafnitrogenstatusofrice. AgronomyJournal, 95,212-217.
[12]	Zhang QJ (张庆江), Zhang LY (张立言), Bi HW (毕桓武) (1997). Theabsorption, accumulationandtranslocationofnitro genandtheirrelationshipstograinproteincontentinspringwheatvariety. ActaAgronomicaSinica (作物学报), 23,712-718. (inChinesewithEnglishabstract).

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