EFFECTS OF ROOT TEMPERATURE ON THERMOSTABILITY OF PHOTOSYNTHETIC APPARATUS IN PRUNUS MIRA SEEDLINGS

doi:10.3773/j.issn.1005-264x.2009.05.018

Abstract

Abstract:

Aims High temperature is one of the major abiotic factors that limit plant growth and development in many regions of the world. Although plant thermo-tolerance has been extensively investigated, little attention has been paid to the effects of root temperature on thermo-stability of the photosynthetic apparatus. Our objective was to investigate the effects of variation in root temperature on photosynthetic apparatus and explore potential mechanisms.
Methods Under high air temperature (37 and 40 ℃), root temperatures of Prunus mira seedlings were increased gradually (variable root temperature) or kept at (25±2) ℃ (appropriate root temperature) throughout the treatment. We examined relative water content, H₂O₂concentration, malondialdehyde (MDA) concentration, activity of antioxidant enzymes, cholorophyll a fluorescence and ABA content in leaves. The experimental design was completely randomized, and each treatment was replicated at least three times.
Important findings The combination of high air temperature and appropriate root temperature induced a decrease in activities of antioxidant enzymes and maximum quantum yield of PSII (F_v/F_m) with relatively high leaf relative water content (RWC). Hydrogen peroxide (H₂O₂) and MDA increased, indicating the photosynthetic apparatus was damaged by high temperature. The combination of high air temperature and gradually increasing root temperature reduced damage to the photosynthetic apparatus with significant improvement in antioxidant enzymes activities and leaf ABA content. Therefore, root temperature plays an important role in enhancing the thermo-stability of the photosynthetic apparatus. First, roots at appropriate temperature maintain high relative water content of leaves, but have damaged photosynthetic apparatus. Second, roots sensed the temperature stimulus and increased the leaf ABA accumulation when root temperature increased gradually, and high leaf ABA level prevented damage to the photosynthetic apparatus and enhanced heat-resistance.

Key words: high temperature stress, antioxidant enzyme, H₂O₂, fluorescence, Prunus mira

HAO Hai-Ping, JIANG Chuang-Dao, SHI Lei, TANG Yu-Dan, YAO Juan, LI Zhi-Qiang. EFFECTS OF ROOT TEMPERATURE ON THERMOSTABILITY OF PHOTOSYNTHETIC APPARATUS IN PRUNUS MIRA SEEDLINGS[J]. Chin J Plant Ecol, 2009, 33(5): 984-992.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2009.05.018

https://www.plant-ecology.com/EN/Y2009/V33/I5/984

Figures/Tables 6

Fig. 1 Sketch map of temperature control container

Fig. 2 Effects of root temperature of Prunus mira seedlings on leaf relative water content (A, B) and Malondialdehyde (MDA) content (C, D) in the leaves of P. mira at high air temperature of 37 or 40 ℃ (mean±SE, n=3)

Fig. 3 Effects of root temperature of Prunus mira seedlings on the initial fluorescence (Fo) (A, B), maximal fluorescence (Fm) (C, D) and maximum quantum yield of PSⅡ photochemistry (Fv/Fm) (E, F) in the leaves of P. mira at 37 ℃ or 40 ℃ (mean±SE, n=6)

Fig. 4 Effects of root temperature of Prunus mira seedlings on H2O2 (dark section) concentration in the P. mira leaves at 37 ℃ (A, B) and 40 ℃ (C, D) for 8 h

Fig. 5 Effects of root temperature of Prunus mira seedlings on specific activity of SOD, POD, APX and CAT in P. mira leaves at 37 ℃ or 40 ℃ (mean±SE, n=3)

Fig. 6 Effects of root temperature of Prunus mira seedlings on ABA content in P. mira leaves at 37 ℃ or 40 ℃ (n=3)

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