Relationships between leaf thickness and vein traits of Achnatherum splendens under different soil moisture conditions in a flood plain wetland, Heihe River, China

doi:10.17521/cjpe.2016.0123

Abstract

Abstract:

Aims The correlations between leaf thickness and vein traits influenced the leaf hydraulic dynamic balance, and there were important meanings to reveal ecophysiological mechanisms of plant leaves water transport and growth rate. Our objective was to study the changes in the relationship between leaf thickness and vein traits (vein diameter, vein density) of Achnatherum splendenspopulations by using standardized major axis estimation (SMA) method under different soil moisture conditions located in flood plain wetland of Zhangye.Methods The study site was located at flood plains wetland of Zhangye, Gansu Province, China. Selecting a starting point along the vertical direction of the river, in turn, along the soil moisture gradient, four plots were set up at intervals of 40 m, plot I (50.07%), plot II (38.77%), plot III (31.5%) and plot IV (20.4%). From each of the four sample plots, seven samples were collected, resulting in (5 m × 5 m) a total of 28 samples. Community traits (height, density) and soil physical and chemical properties were investigated. Six individual samplings of A. splendens from each plot were used to measure the leaf thickness, vein density and vein diameter in laboratory. In addition, the photosynthetically active radiation (PAR), leaf net photosynthetic rate (P_n) and transpiration rate (T_r) of A. splendens were measured in natural environment. The 28 plots were categorized into groups I, II, III and IV, and SMA estimation method was then used to examine the allometric relationship among leaf thickness, vein density and vein diameter. Important findings With a decreased soil moisture, the plant density and height displayed a pattern of steadily declining, while the soil electrical conductivity increased, In addition, the vein density, leaf thickness, water use efficiency (WUE), PAR and twig number of A. splendens displayed a pattern of initial decrease, whereas the vein diameter and T_r increase gradually, P_n and plant high displayed changing trends of increasing-decreasing. The leaf thickness was negatively associated with the vein density, vein diameter, and the relationship varied with the soil moisture conditions (p< 0.05). There was a significant positive relationship (p < 0.05) between the leaf thickness and vein density. The SMA slope of the regression equation gradually decreased and was significantly different from 1.0 (p < 0.05) on plot I and IV. In addition, along decreased soil moisture, the standardized major axis slope of regression equation in the scaling relationships between the leaf thickness and vein diameter gradually increased and was significantly different from -1.0 (p < 0.05) on plot I and IV.

Key words: leaf thickness, vein density, vein diameter, allometry, Achnatherum splendens, flood plain wetland

Ling HAN, Cheng-Zhang ZHAO, Ting XU, Wei FENG, Bei-Bei DUAN. Relationships between leaf thickness and vein traits of Achnatherum splendens under different soil moisture conditions in a flood plain wetland, Heihe River, China[J]. Chin J Plan Ecolo, 2017, 41(5): 529-538.

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References 52

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样地 Plot	土壤含水量 Soil moisture content (%)	土壤电导率 Soil electrical conductivity (ms·cm^-1)	高度 High (cm)	密度 Density (plant·m^-2)	PAR (μmol·m^-2·s^-1)
I	50.07 ± 1.24^a	3.01 ± 0.19^d	171.20 ± 5.24^a	19.5 ± 0.55^a	645.30 ± 13.18^d
II	38.77 ± 1.03^b	5.88 ± 0.23^c	146.37 ± 4.62^b	16.4 ± 0.34^b	833.10 ± 22.48^c
III	31.50 ± 1.01^c	7.79 ± 0.33^b	127.33 ± 3.25^c	10.3 ± 0.24^c	921.40 ± 31.24^b
IV	20.40 ± 0.67^d	11.90 ± 0.55^a	98.57 ± 3.66^d	6.3 ± 0.1^d	1β397.10 ± 45.25^a

样地 Plot	土壤含水量 Soil moisture content (%)	土壤电导率 Soil electrical conductivity (ms·cm^-1)	高度 High (cm)	密度 Density (plant·m^-2)	PAR (μmol·m^-2·s^-1)
I	50.07 ± 1.24^a	3.01 ± 0.19^d	171.20 ± 5.24^a	19.5 ± 0.55^a	645.30 ± 13.18^d
II	38.77 ± 1.03^b	5.88 ± 0.23^c	146.37 ± 4.62^b	16.4 ± 0.34^b	833.10 ± 22.48^c
III	31.50 ± 1.01^c	7.79 ± 0.33^b	127.33 ± 3.25^c	10.3 ± 0.24^c	921.40 ± 31.24^b
IV	20.40 ± 0.67^d	11.90 ± 0.55^a	98.57 ± 3.66^d	6.3 ± 0.1^d	1β397.10 ± 45.25^a

各功能性状 Functional characteristic	样地 Plot
各功能性状 Functional characteristic	I	II	III	IV
株高 Plant height (cm)	130.34 ± 4.50^b	149.67 ± 4.62^a	152.00 ± 5.24^a	95.67 ± 3.56^c
分枝数 Twig number (No.·clump^-1)	203.33 ± 8.83^a	173.67 ± 6.34^b	155.33 ± 5.90^b	113.33 ± 4.29^c
叶片厚度 Leaf thickness (mm)	0.145 ± 0.01^c	0.158 ± 0.02^b	0.162 ± 0.02^b	0.166 ± 0.02^a
叶脉直径 Vein diameter (mm)	0.208 ± 0.04^a	0.195 ± 0.03^b	0.184 ± 0.02^b	0.164 ± 0.02^c
叶脉密度 Vein destiny (mm·mm^-1)	1.283 ± 0.05^c	1.406 ± 0.04^b	1.439 ± 0.03^b	1.587 ± 0.03^a
P_n (μmol CO₂·m^-2·s^-1)	13.20 ± 0.12^c	13.94 ± 0.17^a	14.05 ± 0.18^a	13.87 ± 0.13^b
T_r (mmol H₂O·m^-2·s^-1)	6.83 ± 0.11^a	6.67 ± 0.10^b	6.63 ± 0.09^b	6.35 ± 0.07^c
WUE (μmol CO₂·mmol^-1 H₂O)	1.93 ± 0.01^c	2.08 ± 0.02^b	2.12 ± 0.02^b	2.18 ± 0.03^a

各功能性状 Functional characteristic	样地 Plot
各功能性状 Functional characteristic	I	II	III	IV
株高 Plant height (cm)	130.34 ± 4.50^b	149.67 ± 4.62^a	152.00 ± 5.24^a	95.67 ± 3.56^c
分枝数 Twig number (No.·clump^-1)	203.33 ± 8.83^a	173.67 ± 6.34^b	155.33 ± 5.90^b	113.33 ± 4.29^c
叶片厚度 Leaf thickness (mm)	0.145 ± 0.01^c	0.158 ± 0.02^b	0.162 ± 0.02^b	0.166 ± 0.02^a
叶脉直径 Vein diameter (mm)	0.208 ± 0.04^a	0.195 ± 0.03^b	0.184 ± 0.02^b	0.164 ± 0.02^c
叶脉密度 Vein destiny (mm·mm^-1)	1.283 ± 0.05^c	1.406 ± 0.04^b	1.439 ± 0.03^b	1.587 ± 0.03^a
P_n (μmol CO₂·m^-2·s^-1)	13.20 ± 0.12^c	13.94 ± 0.17^a	14.05 ± 0.18^a	13.87 ± 0.13^b
T_r (mmol H₂O·m^-2·s^-1)	6.83 ± 0.11^a	6.67 ± 0.10^b	6.63 ± 0.09^b	6.35 ± 0.07^c
WUE (μmol CO₂·mmol^-1 H₂O)	1.93 ± 0.01^c	2.08 ± 0.02^b	2.12 ± 0.02^b	2.18 ± 0.03^a