Physiological mechanism of shading stress on photosynthetic efficiency in summer maize (Zea mays)

doi:10.3773/j.issn.1005-264x.2010.12.010

Abstract

Abstract:

Aims Our objective was to investigate the light-adaptive physiological mechanism of maize on shading stress after anthesis, in order to provide theory behind evidence for high and stable yield in summer maize.
Methods In a field experiment, we used common corn (Zea mays) (TY2) to analyze changes of photosynthetic performance, chlorophyll fluorescence parameters, xanthin circle and light partition in maize in the process of shading and recovery stages under (a) shading 55% (+S) 1-20 d after pollination and (b) natural illumination in field condition (-S).
Important findings The net photosynthetic rate and light saturation point of ear leaves declined rapidly, because the chlorophyll content, soluble protein content and PEPCase and RuBPCase activities in ear leaves markedly decreased after shading. Under shading, the maximal photochemical efficiency (F_v/F_m) and the actual photochemical efficiency (Φ_PSII) significantly declined, but non-photochemistry quenching (NPQ) increased. The Φ_PSII of ear leaves increased in early recovery, which indicated that the sudden exposure to natural light significantly enhanced the photosynthetic electron transport rate, according with its photosynthetic rate and carbon metabolism activity of maize ear leaves. After shading, the size of xanthin circle sink in ear leaves was not significantly different, but the de-epoxidation state of xanthin circle (A + Z)/(V + A + Z) increased after shading. The distribution of light energy in photochemical reaction was lower, but the antenna dissipation rate of light energy increased significantly. On the other hand, the dissipation of excess light energy in the recovery process mainly depended on non-photochemical reactions. The PSII primary photochemistry activity of maize decreased under after shading and recovery in early stage, which limited electron supply of photosynthetic carbon metabolism and re- strained the photosynthesis. Then the energy dissipation mostly relied on the xanthin circle increased. The light protective mechanism of maize leaves improved in adaptation to the natural light, and photosynthetic capability gradually recovered after illumination switch.

Key words: deficit light stress, maize, photosynthetic efficiency, physiological trait

JIA Shi-Fang, LI Cong-Feng, DONG Shu-Ting, ZHANG Ji-Wang. Physiological mechanism of shading stress on photosynthetic efficiency in summer maize (Zea mays)[J]. Chin J Plant Ecol, 2010, 34(12): 1439-1447.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2010.12.010

https://www.plant-ecology.com/EN/Y2010/V34/I12/1439

Figures/Tables 8

Table 1 Effect of shading treatments on microclimate of maize population (2005)

处理 Treatment	光辐射强度 Light intensity (μmol·m^-2·s^-1)	群体内不同位置温度 Temperature of different position within population(℃)			CO₂浓度 CO₂ concentration (μmol·mol^-1)
处理 Treatment	光辐射强度 Light intensity (μmol·m^-2·s^-1)	地温 Ground	穗位 Ear	冠层 Canopy	CO₂浓度 CO₂ concentration (μmol·mol^-1)
-S	1 135.9^a	24.9^a	28.9^a	29.4^a	336.2^a
+S	573.3^b	23.1^b	28.5^a	29.0^a	337.3^a

Table 2 Effect of shading treatments on light quality of incident light (2005)

处理 Treatment	波长 Weave length (nm)
处理 Treatment	460	510	560	610	660	680	710	760	810	870	950	1 100
S/CK	42.56^a	36.33^a	37.28^a	38.96^a	36.25^a	37.42^a	38.73^a	39.93^a	39.69^a	40.05^a	38.88^a	42.65^a
Y/CK	41.14^a	35.38^a	36.26^a	36.41^a	36.73^a	36.43^a	39.16^a	41.27^a	40.78^a	40.42^a	42.22^b	46.35^b

Fig. 1 Effect of shading on photosynthetic rate (Pn), chlorophyll content and soluble protein content in maize ear leaf. +S, -S, see Table 1.

Fig.2 The photosynthetic rate-photon flux density (Pn-PFD) curve of maize ear leaf on 10th day after shading treatment (left) and the day of shading finish (right). +S, -S, see Table 1.

Fig. 3 Effect of shading on photosynthesis key enzyme ribulosebisphosphate carboxylase (RuBPCase) and phosphoenolpyruvate carboxylase (PEPCase) in maize ear leaf. +S, -S, see Table 1.

Fig. 4 Effect of shading on chlorophyll fluorescence parameters in maize ear leaf. Fv/Fm, maximal photochemistry efficiency; NPQ, non-photochemical quenching; ФPSII, actual photochemistry efficiency. +S, -S, see Table 1.

Fig. 5 Effect of shading on the xanthophyll cycle in maize ear leaf. A, antheraxanthin; V, violaxanthin; Z, zeaxanthin. +S, -S, see Table 1.

Fig. 6 Effect of shading on PSII photochemistry (P), antenna heat dissipation (D) and excess energy (Ex) in maize ear leaf. +S, -S, see Table 1.

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