Chin J Plan Ecolo ›› 2017, Vol. 41 ›› Issue (6): 622-631.doi: 10.17521/cjpe.2016.0260

• Research Articles • Previous Articles     Next Articles

Effects of localized nitrogen supply treatments on growth and root parameters in Pinus massoniana families under phosphorus deficiency

Ping SONG1, Rui ZHANG1,*(), Zhi-Chun ZHOU1, Jian-She TONG2, Hui WANG2   

  1. 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry
    State Forestry Administration Engineering Research Center of Masson Pine
    Zhejiang Provincial Key Laboratory of Tree Breeding, Hangzhou 311400, China
    2Fuxi Forest Farm, Chun’an, Zhejiang 311701, China;
  • Received:2017-01-03 Accepted:2016-08-09 Online:2017-07-19 Published:2017-06-10
  • Contact: Rui ZHANG
  • About author:

    KANG Jing-yao(1991-), E-mail:


Aims A heterogeneous spatially distribution of nutrients in natural soil may affect plant growth. The objective of this study was to determine the effects of localized nitrogen (N) supply treatments on growth traits and root parameters among different families in Pinus massoniana.Methods Five families of P. massoniana seedlings from full-sib progenies were used as test materials (1, 25, 49, 52, and 57). This study included two conditions, (i.e. homogeneous phosphorus (P) deficiency vs. heterogeneous P efficiency) among soil layers in combination with four N supply treatments in a one-year pot experiment. These N supply treatments were: (1) Homogeneously high N along the soil profile (HHH); (2) high N-high N-low N (HHL); (3) low N-low N-high N (LLH); (4) low N-low N-on side with N addition and the other side without N supply (LLH/L).Important findings This study indicated that localized N supply treatment did enhance the growth of P. massoniana, and this enhancement mainly happened in the pattern of N applied to deep soil. The results showed: 1) Compared to the homogeneous low P condition, there were increase in the growth traits and root parameters of P. massoniana under heterogeneous low P condition. Particularly, the root length and root surface area under the heterogeneous P deficiency condition were 1.95 times and 2.11 times higher than that subjected to the homogeneous P deficiency. 2) Localized N supply treatment affected seedling growth, and there was a significant interaction among N supply pattern and P condition. In compared with homogeneous N supply treatment, the height, basal diameter and dry weight of seedlings increased significantly by localized N supply treatments (LLH and/or LLH/L) under both two P deficiency conditions. But when the seedlings parameters were enhanced under homogeneous P deficiency, they were inhibited under heterogeneous P deficiency subjected to HHL. 3) Within the two P conditions, LLH and LLH/L stimulated root proliferation significantly, and root parameters were significantly enhanced under the heterogeneous P deficiency condition. Specifically, the root length and root surface area subjected to LLH/L rather than HHH were significantly enhanced by 29.2% and 32.3%, respectively. However, the length and surface area of the roots were suppressed by HHL treatment. 4). There were significant differences in response to different N supply treatments among P. massoniana families Seedlings in the families of 49, 52, and 57 responded to the localized N supply treatments with increased root proliferation, which enhanced seedling dry mass. On the other hand, the seedling growth in the family of 25 were stimulated by N and (or) P concentration, while the response of seedlings in the family of 1 to local nitrogen supply was relatively slow and exhibited growth retardation.

Key words: family, localized nitrogen supply, phosphorus deficiency, Pinus massoniana, root parameter

Table 1

Soil physical and chemical properties of the substrate in the pot experiment"

Soil type
Total N (g·kg-1)
Total P (g·kg-1)
Hydrolysable N (mg·kg-1)
Available P (mg·kg-1)
Available K (mg·kg-1)
Organic matter (g·kg-1)
pH value
Acid red soil
0.41 0.34 46.35 0.99 37.78 6.68 5.06

Fig. 1

The patterns of N added under two P deficiency conditions. HHH, homogeneously high N along the soil profile; HHL, high N-high N-low N; LLH, low N-low N-high N; LLH/L, low N-low N-on side with N addition and the other side without N supply."

Table 2

Effects of localized N supply treatments on growth traits, root parameters and N and P concentration of Pinus massoniana (mean ± SE)"

P level
性状 Trait 氮处理 N supply treatment 变异来源 Source of variation (FF value)
HHH HHL LLH LLH/L 家系 Family 氮处理 N 家系&×氮处理
Family &× N
low P
SH (cm) 14.42 &± 0.52b 16.92 &± 0.54a 14.90 &± 0.65b 14.66 &± 0.65b 47.73*** 6.34*** 2.13*
SBD (mm) 2.39 &± 0.10b 2.99 &± 0.07a 2.61 &± 0.09b 2.61 &± 0.09b 23.96*** 12.50*** 2.24*
SDW (g) 1.32 &± 0.12b 1.87 &± 0.10a 1.44 &± 0.13b 1.63 &± 0.12ab 19.21*** 6.11** 2.74*
RDW (g) 0.26 &± 0.02b 0.37 &± 0.03a 0.27 &± 0.02b 0.35 &± 0.02a 12.02*** 7.49*** 1.54
R/S 0.36 &± 0.03a 0.17 &± 0.03b 0.23 &± 0.03ab 0.18 &± 0.03b 12.23*** 2.75* 2.55*
RL (cm) 192.36 &± 9.72a 186.38 &± 11.93a 210.54 &± 14.45a 199.73 &± 11.92a 12.28*** 2.92* 1.20
RSA (cm2) 47.37 &± 3.00b 46.50 &± 2.56b 61.96 &± 4.38a 59.90 &± 3.65a 13.14*** 7.02*** 1.47
PC (mg&·g-1) 0.75 &± 0.03b 1.14 &± 0.04a 0.87 &± 0.08ab 0.95 &± 0.11ab 1.02 0.96 1.02
NC (mg&·g-1) 17.02 &± 0.39b 21.68 &± 2.64a 18.89 &± 0.34ab 16.18 &± 0.59b 3.44** 3.37* 1.47
low P
SH (cm) 17.51 &± 0.74a 13.62 &± 0.52b 16.72 &± 0.67a 16.13 &± 0.67a 43.10*** 12.58*** 2.54*
SBD (mm) 3.18 &± 0.11a 2.61 &± 0.11c 3.11 &± 0.10ab 2.82 &± 0.12bc 17.46*** 8.22*** 2.06*
SDW (g) 2.25 &± 0.19a 1.42 &± 0.13b 2.01 &± 0.14a 1.96 &± 0.16a 15.53*** 6.34*** 2.32*
RDW (g) 0.47 &± 0.04a 0.36 &± 0.03a 0.42 &± 0.04a 0.47 &± 0.06a 13.33*** 1.97 1.04
R/S 0.26 &± 0.03a 0.34 &± 0.05a 0.26 &± 0.02a 0.31 &± 0.04a 7.30*** 0.65 0.49
RL (cm) 368.77 &± 27.72ab 296.81 &± 21.57b 399.17 &± 31.82ab 476.60 &± 61.51a 6.70*** 4.13** 1.09
RSA (cm2) 105.77 &± 7.81b 94.15 &± 7.77b 115.78 &± 9.16ab 139.34 &± 18.01a 8.22*** 3.23* 1.04
PC (mg&·g-1) 0.90 &± 0.03a 1.18 &± 0.04a 1.02 &± 0.03a 1.11 &± 0.08a 1.05 1.06 1.04
NC (mg&·g-1) 17.33 &± 0.39b 18.46 &± 0.34a 15.23 &± 0.41c 15.17 &± 0.39c 15.77*** 23.80*** 1.36

Fig. 2

The seedling growth traits of Pinus massoniana families in different local N supply treatments (mean ± SE). HHH, homogeneously high N along the soil profile; HHL, high N-high N-low N; LLH, low N-low N-high N; LLH/L, low N-low N-on side with N addition and the other side without N supply. Different lower letters indicate significant differences among treatments (p < 0.05)."

Fig. 3

The root parameters of Pinus massoniana families in different local N supply treatments (mean ± SE). HHH, homogeneously high N along the soil profile; HHL, high N-high N-low N; LLH, low N-low N-high N; LLH/L, low N-low N-on side with N addition and the other side without N supply. Different lower letters indicate significant differences among treatments (p < 0.05)."

Fig. 4

N concentration and P concentration of P. massoniana families in different local N supply treatments (mean ± SE). HHH, homogeneously high N along the soil profile; HHL, high N-high N-low N; LLH, low N-low N-high N; LLH/L, low N-low N-on side with N addition and the other side without N supply. Different lower letters indicate significant differences among treatments (p < 0.05)."

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