Chin J Plan Ecolo ›› 2009, Vol. 33 ›› Issue (3): 580-586.doi: 10.3773/j.issn.1005-264x.2009.03.017

• Research Articles • Previous Articles     Next Articles


WEI Ze-Xiu1,4; LIANG Yin-Li1,2*; YAMADA Satoshi3; ZENG Xing-Quan4; ZHOU Mao-Juan1; HUANG Mao-Lin1; WU Yan1   

  1. 1College of Resource and Environment, Northwest Sci &Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China; 2Institute of Water and Soil Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi 712100, China; 3Faculty of Agriculture, Tottori University 4-101 Koyamach-Minama, 680-8553, Japan; 4Tibet Agriculture Research Institute, Lhasa 850032, China
  • Online:2009-05-31 Published:2009-05-31
  • Contact: LIANG Yin-Li

Abstract: Aims Our objective was to investigate diversity within soil microbial communities and tomato yields and qualities under different soil water and fertilization treatments.
Methods PCR (polymerase chain reaction) with long random primers and intron-splice junction primers were used to study greenhouse soil microbial diversity with tomato cultivated under different treatments.
Important findings A total of 182 bands were detected with 6 primer combinations, and 142 (78.02%) of the bands were polymorphic. Soil microbial community diversity was affected by the different soil water contents and fertilizations. Diversity was positively correlated with yields, VC contents and soluble protein contents, but negatively correlated with soluble solids contents. The highest soil microbial community diversity index was detected under the treatment of medium water level and high fertilization; the VC contents, soluble protein contents and yields of tomato fruits were significantly higher and the soluble solids of the tomato fruits was lower than those in other treatments. This showed that treatment can enhance the soil microbial community diversity and stability and to improve soil ecological environments and tomato quality.

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