RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS

doi:10.3773/j.issn.1005-264x.2009.03.017

Abstract

Abstract:

Aims Our objective was to investigate diversity within soil microbial communities and tomato yields and qualities under different soil water and fertilization treatments.

Methods PCR (polymerase chain reaction) with long random primers and intron-splice junction primers were used to study greenhouse soil microbial diversity with tomato cultivated under different treat-ments.

Important findings A total of 182 bands were detected with 6 primer combinations, and 142 (78.02%) of the bands were polymorphic. Soil microbial community diversity was affected by the different soil water contents and fertilizations. Diversity was positively correlated with yields, VC contents and solu-ble protein contents, but negatively correlated with soluble solids contents. The highest soil microbial community diversity index was detected under the treatment of medium water level and high fertilization; the VC contents, soluble protein contents and yields of tomato fruits were significantly higher and the soluble solids of the tomato fruits was lower than those in other treatments. This showed that treatment can enhance the soil microbial community diversity and stability and to improve soil ecological environments and tomato quality.

Key words: intron-splice junction primer, Shannon-Wiener index, yield, quality

WEI Ze-Xiu, LIANG Yin-Li, YAMADA Satoshi, ZENG Xing-Quan, ZHOU Mao-Juan, HUANG Mao-Lin, WU Yan. RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS[J]. Chin J Plant Ecol, 2009, 33(3): 580-586.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2009.03.017

https://www.plant-ecology.com/EN/Y2009/V33/I3/580

Figures/Tables 9

Table 1 The chemical properties of soil layer 0~20 cm in the greenhouse

Fig.1 Mean temperature and RH from April to October in greenhouhouse at Ansai station

Table 2 The sequence of the primers and the result of amplified

Fig.2 The dendrogram of different water and fertiliza-tion treatment based on PCR pattern

Fig.3 2D plots of principal coordinate analysis of six different treatment based on PCR data using NTSYS-pc program Note see Fig.2

Fig.4 The soil microbial Shannon-wiener index of different treatments Note see Fig.2

Fig.5 The amplification map of L1 intron-splice junction primer Note see Fig.2

Table 3 The yield and fruit nutrient quality in different treatments

Table 4 The correlation between soil microbial community diversity index with tomato yield and fruit quality

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