土壤养分斑块对比度改变活血丹克隆整合强度和方向

doi:10.17521/cjpe.2007.0079

摘要/Abstract

摘要：

克隆植物相连片段(或分株)常常生长在不同的土壤养分斑块中。克隆整合使得生长在异质养分斑块中的克隆片段(或分株)产生局部和非局部反应,从而影响相连片段(或分株)的表型可塑性。为了揭示养分斑块对比度对活血丹(Glechoma longituba)克隆整合的影响,在一控制实验中,将活血丹克隆片断种植于4种不同对比度的环境中,即:无对比度(对照)、低对比度、中对比度和高对比度。活血丹在气体交换、水势、荧光、形态、生长与分配方面的克隆整合强度随养分斑块对比度的增强而表现出增强或减弱的变化趋势;养分斑块对比度越强,活血丹气体交换和荧光暗反应的整合强度越小,叶片水势整合强度越大。斑块对比度可改变部分性状的克隆整合方向;超过一定的对比阈值,整合强度随养分对比度的变化趋势会向着相反方向转变。克隆整合对生理特征的修饰幅度小于对生长特征的修饰幅度。这些结果指示:养分斑块对比度可通过修饰克隆整合格局(即强度和方向)而改变克隆植物的表型可塑性。

关键词: 克隆可塑性, 气体交换, 生理整合, 生长与分配, 养分斑块, 荧光特征

Abstract:

Aims The connected fragments of clonal plants often grow in different soil-nutrient patches, and clonal integration can modify the plasticity of these connected clonal fragments in diverse ways. We address how nutrient patch contrast modifies intensity and direction of clonal integration.
Methods In a common garden experiment, we planted clonal fragments of Glechoma longituba in four nutrient-patch contrast conditions: no (control), low, medium and high contrast. Water was supplied when necessary. We measured physiological traits at peak growth and harvested all plant materials at the end of the experiment, which ran from July 19 through September 30, 2005. Data were analyzed with SPSS software.
Important findings Clonal integration in gas exchange, water potential, fluorescence, growth and biomass allocation differed among nutrient patch contrast treatments. Higher contrast had smaller integration in both gas exchange and dark fluorescence response and greater integration in leaf water potential. Direction of clonal integration for some characteristics also different among treatments, indicating that the threshold of soil-nutrient patch contrast might play a fundamental role in determining integration direction. Clonal integration had far broader effects on growth and allocation than physiological traits. We infer that nutrient-patch contrast may modify the intensity and direction of clonal integration, which in turn can shape the phenotypic plasticity of clonal plants.

Key words: clonal plasticity, fluorescence, gas exchange, growth and allocation, nutrient patches, physiological integration

张丽丽, 董鸣, 李仁强, 王艳红, 崔清国, 何维明. 土壤养分斑块对比度改变活血丹克隆整合强度和方向. 植物生态学报, 2007, 31(4): 619-624. DOI: 10.17521/cjpe.2007.0079

ZHANG Li-Li, DONG Ming, LI Ren-Qiang, WANG Yan-Hong, CUI Qing-Guo, HE Wei-Ming. SOIL-NUTRIENT PATCH CONTRAST MODIFIES INTENSITY AND DIRECTION OF CLONAL INTEGRATION IN GLECHOMA LONGITUBA. Chinese Journal of Plant Ecology, 2007, 31(4): 619-624. DOI: 10.17521/cjpe.2007.0079

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2007.0079

https://www.plant-ecology.com/CN/Y2007/V31/I4/619

图/表 4

图1 实验设计示意图(生长槽中点的浓淡深浅表征养分浓度的高低)

Fig.1 Layout of the experiment (The four different patterns stand for four different nutrient patches) C: Control LC: Low contrast MC: Medium contrast HC: high contrast

表1 不同对比养分斑块中的养分状况

Table 1 Total amount of nutrients used in the four contrasting nutrient patches

	养分总量 Total amount of nutrients (g·m^-3)
	对照 Control (C)		低对比强度 Low contrast (LC)		中对比强度 Medium contrast (MC)		高对比强度 High contrast (HC)
	O	T	O	T	O	T	O	T
N	1 125	1 125	1 125	1 000	1 125	875	1 125	750
P	1 125	1 125	1 125	1 000	1 125	875	1 125	750
K	1 125	1 125	1 125	1 000	1 125	875	1 125	750

图2 不同养分斑块对比度下气体交换(A),水势(B)和荧光反应(C, D)的克隆整合格局

Fig.2 Clonal integration (I) in physiological function along a nutrient-patch contrast gradient Pn:叶片净光合速率 Net photosynthetic rate Gs:气孔导度 Stomatal conductance Ci/Ca:胞内外CO2浓度比 Ratio of substomatal CO2 to atmospheric CO2 E:蒸腾速率 Transpiration rate F:最小荧光Minimal fluorescence in the light conditions Fm:最大荧光 Maximal fluorescence in the light conditions Yield:最大光化学量子产量 Fluorescence efficiency of PSⅡ in the light conditions qP:光化学淬灭系数 Photochemical quenching in the light conditions NPQ:非光化学淬灭系数 Nonphotochemical quenching in the light conditions F':最小荧光 Minimal fluorescence in the dark conditions Fm':最大荧光 Maximal fluorescence in the dark conditions Yield':最大光化学量子产量 Fluorescence efficiency of PSⅡin the dark conditions C、LC、MC、HC:同图1 See Fig.1

图3 养分斑块对比度对生长与分配(A)和比生物量分配(B)整合强度的影响

Fig.3 Clonal integration (I) in biomass growth and allocation (A) and specific biomass allocation (B) along a nutrient-patch contrast gradient F:克隆片段生物量 Fragment biomass LWR:叶片生物量比 Lamina weight ratio PWR:叶柄生物量比 Petiole weight ratio SWR:匍匐茎生物量比 Stolon weight ratio RWR:根生物量比 Root weight ratio R/S:根冠比 Root/shoot ratio SLA:比叶面积 Specific lamina area SPL:比叶柄长 Specific petiole length SSL:比茎长 Specific stolon length SRL:比根长 Specific root length C、LC、MC、HC:同图1 See Fig.1

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