植物生态学报 ›› 2022, Vol. 46 ›› Issue (1): 88-101.DOI: 10.17521/cjpe.2021.0206
所属专题: 光合作用
杨丽婷, 谢燕燕, 左珂怡, 徐森, 谷瑞, 陈双林, 郭子武()
收稿日期:
2021-05-31
接受日期:
2021-10-08
出版日期:
2022-01-20
发布日期:
2022-04-13
通讯作者:
郭子武
作者简介:
*(hunt-panther@163.com)基金资助:
Li-Ting YANG, Yan-Yan XIE, Ke-Yi ZUO, Sen XU, Rui GU, Shuang-Lin CHEN, Zi-Wu GUO()
Received:
2021-05-31
Accepted:
2021-10-08
Online:
2022-01-20
Published:
2022-04-13
Contact:
Zi-Wu GUO
Supported by:
摘要:
分株数量或生物量比例差异会明显影响克隆系统对资源异质性环境的生态适应性, 地下茎木质化、连接稳固的竹类植物在生长过程中相连克隆分株通常会生活在异质光环境中, 但其叶片光合生理特性对异质光环境的响应及其分株比例效应则未见报道。该研究以地下茎相连的美丽箬竹(Indocalamus decorus)克隆系统为实验材料, 设置2个遮光率(分别为50% ± 5%和75% ± 5%)和3个分株比例(遮光与未遮光分株比例分别为1:3、2:2、3:1)处理, 分株数量为4株。分别测定了遮光处理后30、90、150天遮光和未遮光分株叶片光响应特征、气体交换参数、光合色素含量, 分析了异质光环境下美丽箬竹光合生理的变化规律。结果显示: 分株比例对美丽箬竹光合生理有显著影响, 且其与遮光、处理时间交互作用显著。美丽箬竹克隆系统遮光分株比例越大, 即遮光相对分株数量越多, 其表观量子效率(AQE)、光饱和点、最大净光合速率(Pn max)、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、水分利用效率越大, 光补偿点、暗呼吸速率越小, 其光合效率越高, 光能利用能力越强, 而与之相连的未遮光分株则相反; 随遮光分株比例的增大, 遮光分株叶片叶绿素a、叶绿素b含量呈先升高而后下降的变化趋势, 类胡萝卜素(Car)含量则持续下降, 而与之相连的未遮光分株叶片光合色素含量则呈下降趋势; 遮光率提高, 相同分株比例美丽箬竹克隆系统遮光分株叶片AQE、Pn max、Pn、Gs、胞间CO2浓度(Ci)和光合色素含量总体升高, 而与之相连的未遮光分株叶片Pn max、Ci以及Car含量则总体下降。研究结果表明异质光环境下, 遮光分株光合效率和弱光利用能力明显增强, 而未遮光分株则相反, 克隆系统内分株间发生了明显的克隆分工, 且2:2、3:1分株比例克隆系统较1:3分株比例对异质光环境具有更好的适应能力。美丽箬竹克隆系统可通过差异性调节分株光合生理特性和光合色素含量, 提高遮光分株光能利用和光合效率来适应异质光环境, 以提高克隆系统的适合度。
杨丽婷, 谢燕燕, 左珂怡, 徐森, 谷瑞, 陈双林, 郭子武. 分株比例对异质光环境下美丽箬竹克隆系统光合生理的影响. 植物生态学报, 2022, 46(1): 88-101. DOI: 10.17521/cjpe.2021.0206
Li-Ting YANG, Yan-Yan XIE, Ke-Yi ZUO, Sen XU, Rui GU, Shuang-Lin CHEN, Zi-Wu GUO. Effects of ramet ratio on photosynthetic physiology of Indocalamus decorus clonal system under heterogeneous light environment. Chinese Journal of Plant Ecology, 2022, 46(1): 88-101. DOI: 10.17521/cjpe.2021.0206
图1 美丽箬竹克隆系统实验设计示意图。 1:3、2:2、3:1分别代表遮光分株数量与未遮光分株数量之比。
Fig. 1 Schematic diagram of the experimental design of Indocalamus decorus clonal system exposed to different shading levels. 1:3, 2:2 and 3:1 referred to the ratio of shaded ramets to unshaded ones, respectively.
变异来源 Variation source | df | AQE | LCP | LSP | Pn max | Rd | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 26.7 | <0.001 | 19.1 | <0.001 | 24.6 | <0.001 | 8.7 | 0.001 | 12.2 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 6.3 | 0.017 | 7.5 | 0.010 | 32.4 | <0.001 | 5.3 | 0.027 | 0.1 | 0.763 |
处理时间 Treatment time (T) | 2, 53 | 79.6 | <0.001 | 59.8 | <0.001 | 255.6 | <0.001 | 151.6 | <0.001 | 16.7 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 9.0 | 0.001 | 1.8 | 0.181 | 3.5 | 0.042 | 0.9 | 0.420 | 9.7 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 4.6 | 0.004 | 3.9 | 0.010 | 2.4 | 0.073 | 0.3 | 0.896 | 2.8 | 0.041 |
遮光率×处理时间 S × T | 2, 53 | 8.3 | 0.001 | 14.2 | <0.001 | 1.4 | 0.261 | 4.8 | 0.014 | 34.0 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 7.1 | <0.001 | 5.0 | 0.002 | 7.3 | <0.001 | 1.5 | 0.213 | 3.2 | 0.025 |
表1 异质光环境下美丽箬竹克隆系统遮光分株叶片光响应参数三因素方差分析
Table 1 Statistical results of three-way ANOVA for leaf light response parameters of shaded ramets of Indocalamus decorus clonal system under heterogeneous light environment
变异来源 Variation source | df | AQE | LCP | LSP | Pn max | Rd | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 26.7 | <0.001 | 19.1 | <0.001 | 24.6 | <0.001 | 8.7 | 0.001 | 12.2 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 6.3 | 0.017 | 7.5 | 0.010 | 32.4 | <0.001 | 5.3 | 0.027 | 0.1 | 0.763 |
处理时间 Treatment time (T) | 2, 53 | 79.6 | <0.001 | 59.8 | <0.001 | 255.6 | <0.001 | 151.6 | <0.001 | 16.7 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 9.0 | 0.001 | 1.8 | 0.181 | 3.5 | 0.042 | 0.9 | 0.420 | 9.7 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 4.6 | 0.004 | 3.9 | 0.010 | 2.4 | 0.073 | 0.3 | 0.896 | 2.8 | 0.041 |
遮光率×处理时间 S × T | 2, 53 | 8.3 | 0.001 | 14.2 | <0.001 | 1.4 | 0.261 | 4.8 | 0.014 | 34.0 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 7.1 | <0.001 | 5.0 | 0.002 | 7.3 | <0.001 | 1.5 | 0.213 | 3.2 | 0.025 |
变异来源 Variation source | df | AQE | LCP | LSP | Pn max | Rd | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 31.6 | <0.001 | 10.3 | <0.001 | 76.5 | <0.001 | 28.9 | <0.001 | 32.5 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 38.4 | <0.001 | 41.6 | <0.001 | 33.1 | <0.001 | 3.8 | 0.062 | 8.1 | 0.007 |
处理时间 Treatment time (T) | 2, 53 | 174.6 | <0.001 | 55.8 | <0.001 | 120.9 | <0.001 | 270.9 | <0.001 | 117.5 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 0.9 | 0.427 | 6.0 | 0.006 | 3.2 | 0.054 | 2.6 | 0.089 | 10.2 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 2.7 | 0.048 | 4.1 | 0.008 | 8.1 | <0.001 | 5.5 | 0.001 | 2.3 | 0.080 |
遮光率×处理时间 S × T | 2, 53 | 3.0 | 0.064 | 34.2 | <0.001 | 15.5 | <0.001 | 9.9 | <0.001 | 2.3 | 0.110 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 0.3 | 0.909 | 8.1 | <0.001 | 15.6 | <0.001 | 4.1 | 0.008 | 2.5 | 0.059 |
表2 异质光环境下美丽箬竹克隆系统未遮光分株叶片光响应参数三因素方差分析
Table 2 Statistical results of three-way ANOVA for leaf light response parameters of unshaded ramets of Indocalamus decorus clonal system under heterogeneous light environment
变异来源 Variation source | df | AQE | LCP | LSP | Pn max | Rd | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 31.6 | <0.001 | 10.3 | <0.001 | 76.5 | <0.001 | 28.9 | <0.001 | 32.5 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 38.4 | <0.001 | 41.6 | <0.001 | 33.1 | <0.001 | 3.8 | 0.062 | 8.1 | 0.007 |
处理时间 Treatment time (T) | 2, 53 | 174.6 | <0.001 | 55.8 | <0.001 | 120.9 | <0.001 | 270.9 | <0.001 | 117.5 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 0.9 | 0.427 | 6.0 | 0.006 | 3.2 | 0.054 | 2.6 | 0.089 | 10.2 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 2.7 | 0.048 | 4.1 | 0.008 | 8.1 | <0.001 | 5.5 | 0.001 | 2.3 | 0.080 |
遮光率×处理时间 S × T | 2, 53 | 3.0 | 0.064 | 34.2 | <0.001 | 15.5 | <0.001 | 9.9 | <0.001 | 2.3 | 0.110 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 0.3 | 0.909 | 8.1 | <0.001 | 15.6 | <0.001 | 4.1 | 0.008 | 2.5 | 0.059 |
图2 异质光环境下美丽箬竹克隆系统遮光分株叶片光响应特征(平均值±标准差)。 1:3、2:2、3:1为分株比例; 50%、75%为遮光率。大写字母表示同一处理相同指标不同时间比较, 小写字母表示不同处理相同指标同一时间比较, 字母不同表示差异显著(p < 0.05)。
Fig. 2 Light response characteristics of shaded ramets of Indocalamus decorus clonal system under heterogeneous light environment (mean ± SD). 1:3, 2:2 and 3:1 referred to the ratio of shaded ramets to unshaded ones, respectively. The shading rate is 50% and 75%, respectively. AQE, apparent quantum efficiency; LCP, light compensation point; LSP, light saturation point; Pn max, maximum net photosynthetic rate; Rd, dark respiration rate. Uppercase letters indicated the comparison between different treatment times for the same treatment; lowercase letters indicated the comparison between the different treatments at the same time, different letters represented significant difference at the level of p < 0.05.
图3 异质光环境下美丽箬竹克隆系统未遮光分株叶片光响应特征(平均值±标准差)。 1:3、2:2、3:1为分株比例; 0% (50%), 与遮光50%分株相连的未遮光分株; 0% (75%), 与遮光75%分株相连的未遮光分株。大写字母表示同一处理相同指标不同时间比较, 小写字母表示不同处理相同指标同一时间比较, 字母不同表示差异显著(p < 0.05)。
Fig. 3 Light response characteristics of unshaded ramets of Indocalamus decorus clonal system under heterogeneous light environment (mean ± SD). 1:3, 2:2 and 3:1 referred to the ratio of shaded ramets to unshaded ones, respectively; 0% (50%), unshaded ramets connected to 50% shaded ramets; 0% (75%), unshaded ramets connected to 75% shaded ramets. AQE, apparent quantum efficiency; LCP, light compensation point; LSP, light saturation point; Pn max, maximum net photosynthetic rate; Rd, dark respiration rate. Uppercase letters indicated the comparison between the different treatment times for the same treatment; lowercase letters indicated the comparison between the different treatments at the same time, different letters represented significant difference at the level of p < 0.05.
变异来源 Variation source | df | Pn | Gs | Ci | Tr | WUE | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 33.4 | <0.001 | 180.2 | <0.001 | 4.9 | 0.013 | 16.9 | <0.001 | 47.1 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 3.4 | 0.072 | 12.4 | 0.001 | 7.1 | 0.011 | 1.6 | 0.220 | 112.1 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 240.5 | <0.001 | 5 486.6 | <0.001 | 73.0 | <0.001 | 154.2 | <0.001 | 221.6 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 14.5 | <0.001 | 596.0 | <0.001 | 2.9 | 0.066 | 10.4 | <0.001 | 4.8 | 0.014 |
分株比例×处理时间 R × T | 4, 53 | 14.2 | <0.001 | 26.5 | <0.001 | 2.2 | 0.089 | 33.6 | <0.001 | 1.2 | 0.318 |
遮光率×处理时间 S × T | 2, 53 | 6.9 | 0.003 | 609.4 | <0.001 | 16.8 | <0.001 | 8.7 | 0.001 | 59.1 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 7.0 | <0.001 | 36.9 | <0.001 | 2.2 | 0.087 | 3.1 | 0.026 | 12.6 | <0.001 |
表3 异质光环境下美丽箬竹克隆系统遮光分株叶片气体交换参数三因素方差分析
Table 3 Statistical results of three-way ANOVA for leaf photosynthetic parameters of shaded ramets of Indocalamus decorus clonal system under heterogeneous light environment
变异来源 Variation source | df | Pn | Gs | Ci | Tr | WUE | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 33.4 | <0.001 | 180.2 | <0.001 | 4.9 | 0.013 | 16.9 | <0.001 | 47.1 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 3.4 | 0.072 | 12.4 | 0.001 | 7.1 | 0.011 | 1.6 | 0.220 | 112.1 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 240.5 | <0.001 | 5 486.6 | <0.001 | 73.0 | <0.001 | 154.2 | <0.001 | 221.6 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 14.5 | <0.001 | 596.0 | <0.001 | 2.9 | 0.066 | 10.4 | <0.001 | 4.8 | 0.014 |
分株比例×处理时间 R × T | 4, 53 | 14.2 | <0.001 | 26.5 | <0.001 | 2.2 | 0.089 | 33.6 | <0.001 | 1.2 | 0.318 |
遮光率×处理时间 S × T | 2, 53 | 6.9 | 0.003 | 609.4 | <0.001 | 16.8 | <0.001 | 8.7 | 0.001 | 59.1 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 7.0 | <0.001 | 36.9 | <0.001 | 2.2 | 0.087 | 3.1 | 0.026 | 12.6 | <0.001 |
变异来源 Variation source | df | Pn | Gs | Ci | Tr | WUE | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 99.2 | <0.001 | 344.6 | <0.001 | 16.8 | <0.001 | 18.9 | <0.001 | 47.5 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 15.0 | <0.001 | 62.0 | <0.001 | 22.6 | <0.001 | 0.7 | 0.412 | 63.9 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 984.4 | <0.001 | 2 657.9 | <0.001 | 172.4 | <0.001 | 73.7 | <0.001 | 305.8 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 92.8 | <0.001 | 151.2 | <0.001 | 18.3 | <0.001 | 6.6 | 0.004 | 167.4 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 4.9 | 0.003 | 43.4 | <0.001 | 4.3 | 0.006 | 2.3 | 0.082 | 26.4 | <0.001 |
遮光率×处理时间 S × T | 2, 53 | 23.8 | <0.001 | 366.9 | <0.001 | 9.3 | 0.001 | 4.1 | 0.026 | 52.9 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 50.7 | <0.001 | 88.0 | <0.001 | 4.2 | 0.007 | 5.7 | 0.001 | 31.5 | <0.001 |
表4 异质光环境下美丽箬竹克隆系统未遮光分株叶片气体交换参数三因素方差分析
Table 4 Statistical results of three-way ANOVA for leaf photosynthetic parameters of unshaded ramets of Indocalamus decorus clonal system under heterogeneous light environment
变异来源 Variation source | df | Pn | Gs | Ci | Tr | WUE | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 99.2 | <0.001 | 344.6 | <0.001 | 16.8 | <0.001 | 18.9 | <0.001 | 47.5 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 15.0 | <0.001 | 62.0 | <0.001 | 22.6 | <0.001 | 0.7 | 0.412 | 63.9 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 984.4 | <0.001 | 2 657.9 | <0.001 | 172.4 | <0.001 | 73.7 | <0.001 | 305.8 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 92.8 | <0.001 | 151.2 | <0.001 | 18.3 | <0.001 | 6.6 | 0.004 | 167.4 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 4.9 | 0.003 | 43.4 | <0.001 | 4.3 | 0.006 | 2.3 | 0.082 | 26.4 | <0.001 |
遮光率×处理时间 S × T | 2, 53 | 23.8 | <0.001 | 366.9 | <0.001 | 9.3 | 0.001 | 4.1 | 0.026 | 52.9 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 50.7 | <0.001 | 88.0 | <0.001 | 4.2 | 0.007 | 5.7 | 0.001 | 31.5 | <0.001 |
图4 异质光环境下美丽箬竹克隆系统遮光分株叶片光合气体交换特征(平均值±标准差)。 1:3、2:2、3:1为分株比例; 50%、75%为遮光率。大写字母表示同一处理相同指标不同时间比较, 小写字母表示不同处理相同指标同一时间比较, 字母不同表示差异显著(p < 0.05)。
Fig. 4 Photosynthetic parameters of shaded ramets of Indocalamus decorus clonal system under heterogeneous light environment (mean ± SD). 1:3, 2:2 and 3:1 referred to the ratio of shaded ramets to unshaded ones, respectively. The shading rate is 50% and 75%, respectively. Ci, intercellular CO2 concentration; Gs, stomatal conductance; Pn, net photosynthetic rate; Tr, transpiration rate; WUE, water use efficiency. Uppercase letters indicated the comparison between the different treatment times for the same treatment; lowercase letters indicated the comparison between the different treatments at the same time, different letters represented significant difference at the level of p < 0.05.
图5 异质光环境下美丽箬竹克隆系统未遮光分株叶片光合气体交换特征(平均值±标准差)。 1:3、2:2、3:1为分株比例; 0% (50%), 与遮光50%分株相连的未遮光分株; 0% (75%), 与遮光75%分株相连的未遮光分株。大写字母表示同一处理相同指标不同时间比较, 小写字母表示不同处理相同指标同一时间比较, 字母不同表示差异显著(p < 0.05)。
Fig. 5 Photosynthetic parameters of unshaded ramets of Indocalamus decorus clonal system under heterogeneous light environment (mean ± SD). 1:3, 2:2, and 3:1 referred to the ratios of shaded ramets to unshaded ones, respectively. 0% (50%), unshaded ramets connected to 50% shading ramets; 0% (75%), unshaded ramets connected to 75% shading ramets. Ci, intercellular CO2 concentration; Gs, stomatal conductance; Pn, net photosynthetic rate; Tr, transpiration rate; WUE, water use efficiency. Uppercase letters indicated the comparison between the different treatment times for the same treatment; lowercase letters indicated the comparison between the different treatments at the same time, different letters represented significant difference at the level of p < 0.05.
变异来源 Variation source | df | Chl a | Chl b | Car | |||
---|---|---|---|---|---|---|---|
F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 111.7 | <0.001 | 50.7 | <0.001 | 43.5 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 131.5 | <0.001 | 318.3 | <0.001 | 75.5 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 61.7 | <0.001 | 140.2 | <0.001 | 149.1 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 3.2 | 0.053 | 0.3 | 0.733 | 31.9 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 9.5 | <0.001 | 3.4 | 0.019 | 5.0 | 0.003 |
遮光率×处理时间 S × T | 2, 53 | 8.7 | 0.001 | 35.5 | <0.001 | 58.7 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 12.5 | <0.001 | 9.5 | <0.001 | 9.2 | <0.001 |
表5 异质光环境下美丽箬竹克隆系统遮光分株叶片光合色素含量三因素方差分析
Table 5 Statistical results of three-way ANOVA for photosynthetic pigment content of shaded ramets of Indocalamus decorus clonal system under heterogeneous light environment
变异来源 Variation source | df | Chl a | Chl b | Car | |||
---|---|---|---|---|---|---|---|
F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 111.7 | <0.001 | 50.7 | <0.001 | 43.5 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 131.5 | <0.001 | 318.3 | <0.001 | 75.5 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 61.7 | <0.001 | 140.2 | <0.001 | 149.1 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 3.2 | 0.053 | 0.3 | 0.733 | 31.9 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 9.5 | <0.001 | 3.4 | 0.019 | 5.0 | 0.003 |
遮光率×处理时间 S × T | 2, 53 | 8.7 | 0.001 | 35.5 | <0.001 | 58.7 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 12.5 | <0.001 | 9.5 | <0.001 | 9.2 | <0.001 |
变异来源 Variation source | df | Chl a | Chl b | Car | |||
---|---|---|---|---|---|---|---|
F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 415.3 | <0.001 | 314.7 | <0.001 | 272.4 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 0.7 | 0.404 | 31.9 | <0.001 | 28.2 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 298.8 | <0.001 | 350.3 | <0.001 | 211.2 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 8.7 | 0.001 | 11.8 | <0.001 | 15.7 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 17.2 | <0.001 | 8.6 | <0.001 | 12.1 | <0.001 |
遮光率×处理时间 S × T | 2, 53 | 9.9 | <0.001 | 19.4 | <0.001 | 52.2 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 44.5 | <0.001 | 48.8 | <0.001 | 50.0 | <0.001 |
表6 异质光环境下美丽箬竹未遮光分株叶片光合色素含量三因素方差分析
Table 6 Statistical results of three-way ANOVA for photosynthetic pigment content of unshaded ramets of Indocalamus decorus clonal system under heterogeneous light environment
变异来源 Variation source | df | Chl a | Chl b | Car | |||
---|---|---|---|---|---|---|---|
F | p | F | p | F | p | ||
分株比例 Ramet ratio (R) | 2, 53 | 415.3 | <0.001 | 314.7 | <0.001 | 272.4 | <0.001 |
遮光率 Shading rate (S) | 1, 53 | 0.7 | 0.404 | 31.9 | <0.001 | 28.2 | <0.001 |
处理时间 Treatment time (T) | 2, 53 | 298.8 | <0.001 | 350.3 | <0.001 | 211.2 | <0.001 |
分株比例×遮光率 R × S | 2, 53 | 8.7 | 0.001 | 11.8 | <0.001 | 15.7 | <0.001 |
分株比例×处理时间 R × T | 4, 53 | 17.2 | <0.001 | 8.6 | <0.001 | 12.1 | <0.001 |
遮光率×处理时间 S × T | 2, 53 | 9.9 | <0.001 | 19.4 | <0.001 | 52.2 | <0.001 |
分株比例×遮光率×处理时间 R × S × T | 4, 53 | 44.5 | <0.001 | 48.8 | <0.001 | 50.0 | <0.001 |
图6 异质光环境下美丽箬竹克隆系统遮光分株(A、C、E)与未遮光分株(B、D、F)叶片光合色素含量(平均值±标准差)。 1:3、2:2、3:1为分株比例; 50%、75%为遮光率; 0% (50%), 与遮光50%分株相连的未遮光分株; 0% (75%), 与遮光75%分株相连的未遮光分株。大写字母表示同一处理相同指标不同时间比较, 小写字母表示不同处理相同指标同一时间比较。字母不同表示差异显著(p < 0.05)。
Fig. 6 Photosynthetic pigment content of shaded ramets (A, C, E) and unshaded ramets (B, D, F) of Indocalamus decorus clonal system under heterogeneous light environment (mean ± SD). 1:3, 2:2 and 3:1 referred to the ratios of shaded ramets to unshaded ones, respectively. The shading rate is 50% and 75%, respectively. 0% (50%), unshaded ramets connected to 50% shaded ramets; 0% (75%), unshaded ramets connected to 75% shaded ramets. Chl a, chlorophyll a; Chl b, chlorophyll b; Car, carotenoid. Uppercase letters indicated the comparison between the different treatment times for the same treatment; lowercase letters indicated the comparison between the different treatments at the same time, different letters represented significant difference at the level of p < 0.05.
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