植物生态学报 ›› 2018, Vol. 42 ›› Issue (4): 498-507.DOI: 10.17521/cjpe.2017.0320

• 研究论文 • 上一篇    下一篇

最大电子传递速率的确定及其对电子流分配的影响

叶子飘1,段世华2,安婷1,康华靖3,*()   

  1. 1 井冈山大学数理学院, 江西吉安 343009
    2 井冈山大学生命科学学院, 江西吉安 343009
    3 温州市农业科学研究院, 浙江温州 325006
  • 出版日期:2018-04-20 发布日期:2018-03-21
  • 通讯作者: 康华靖
  • 基金资助:
    国家自然科学基金项目(31560069)

Determination of maximum electron transport rate and its impact on allocation of electron flow

Zi-Piao YE1,Shi-Hua DUAN2,Ting AN1,Hua-Jing KANG3,*()   

  1. 1 College of Math and Physics, Jinggangshan University, Ji'an, Jiangxi 343009, China
    2 School of Life Sciences, Jinggangshan University, Ji'an, Jiangxi 343009, China
    3 Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang 325006, China
  • Online:2018-04-20 Published:2018-03-21
  • Contact: Hua-Jing KANG
  • Supported by:
    Supported by the National Natural Science Foundation of China (31560069).

摘要:

非直角双曲线模型(简称模型I)是Farquhar、von Caemmerer和Berry提出的生物化学光合模型(简称FvCB生化模型)的主要子模型。在植物光合作用对光响应曲线的拟合中, 模型I得到广泛的应用和验证。同时, 模型I也可用于估算植物叶片的最大电子传递速率(Jmax)。然而, 由模型I估算植物叶片的Jmax是否与实测值相符, 尚未得到严格的验证。该文应用LI-6400-40光合测定仪测定了遮阴和全日照条件下大豆(Glycine max)叶片的光合速率和电子传递速率对光的响应曲线, 然后分别用模型I和电子传递速率对光响应机理模型(简称模型II)进行了拟合。结果表明, 由模型I估算遮阴和全日照条件下大豆叶片的Jmax与观测值之间存在显著差异; 由模型II计算得到的Jmax与实测值之间不存在显著差异。此外, 用模型I估算的Jmax将高估光合电子流分配到光呼吸的量, 从而高估光呼吸对植物的光保护作用。因此, 在估算植物叶片Jmax和准确评估光呼吸对植物光保护作用方面, 模型II更合理。

关键词: 大豆, 非直角双曲线模型, 光响应机理模型, FvCB生化模型, 电子传递速率

Abstract:

Aims The non-rectangular hyperbolic model (termed as model I) is the main submodel of the FvCB biochemical model, which is used to estimate the maximum electron transport rate (Jmax) of plant leaves. The submodel is widely applied to fit the light-response curves of electron transport rate (J-I curves), and obtain Jmax. However, it has not been strictly verified whether Jmax calculated by model I is consistent with the measured values.

Methods Light-response curves of electron transport rate and of photosynthesis rate of soybean (Glycine max) (under shading and full sunlight) were simultaneously measured by LI-6400-40, then these data were simulated by model I and the mechanistic model of light-response of electron transport rate (termed as model II).

Important findings The results showed that there was the significant differences between Jmax estimated by model I and the observation data irrespective of shading and full sunny leaves of soybean. However, there was no significant difference between Jmax calculated by model II and the measured value. Because Jmax was overestimated by model I, it must lead to overestimate the amount of photosynthetic electron flow to allocate to photorespiration pathway, and magnify the photoprotection of photorespiration on plants. On the contrary, the Jmax and saturation light intensity (PARsat) obtained by the model II were in very close agreement with the observations. It can be concluded that the model II was superior to the model I in estimates of Jmax and PARsat. Therefore, we recommend model II to be used as an operational model for fitting J-I curves and accurately assess the role of photorespiration on plant photo-protection.

Key words: Glycine max, non-rectangular hyperbolic model, mechanistic model, FvCB biochemical model, electron transport rate