NO和H2O2诱导大豆根尖和边缘细胞耐铝反应的 作用

doi:10.3724/SP.J.1258.2011.00981

摘要/Abstract

摘要：

NO和H₂O₂是参与植物抗非生物胁迫反应的重要信号分子, 为了确定NO和H₂O₂在大豆(Glycine max)根尖和根边缘细胞(root border cells, RBCs)耐铝反应中的作用及其相互关系, 以‘浙春3号’大豆为材料, 研究了铝毒胁迫下大豆根尖内源NO和H₂O₂的变化, 以及外源NO和H₂O₂诱导大豆根尖和RBCs的耐铝反应。结果表明, 50 μmol·L^-1 Al处理48 h显著抑制大豆根的伸长, 提高Al在根尖的积累, 同时显著增加根尖内源NO和H₂O₂含量。施加0.25 mmol·L^-1外源NO供体亚硝基铁氰化钠(Na₂[Fe(CN)₅NO]·2H₂O, sodium nitroprusside, SNP)和0.1 mmol·L^-1H₂O₂, 能有效地缓解Al对大豆根伸长的抑制、根尖Al积累和RBCs的死亡, 该缓解作用可以被0.05 mmol·L^-1NO清除剂2-(4-羧基苯)-4,4,5,5-四甲基咪唑-1-氧-3-氧化物,钾盐 (C₁₄H₁₆N₂O₄·K, carboxy-PTIO, cPTIO)和150 U·mL^-1H₂O₂清除酶(catalase, CAT)逆转。并且外源NO能够显著促进根尖H₂O₂的积累, 而外源H₂O₂对根尖NO的含量无显著影响。这表明NO和H₂O₂是诱导大豆根尖及RBCs耐铝反应的两种信号分子, NO可能通过调控H₂O₂的形成, 进而诱导大豆根尖及RBCs的耐铝反应。

关键词: 铝毒, H₂O₂, NO, 根尖, 根边缘细胞, 大豆

Abstract:

Aims Nitric oxide (NO) and hydrogen peroxide (H₂O₂) function as signaling molecules in plants. A role for NO and H₂O₂in the regulation of many abiotic stress responses, including drought, salt, heat, heavy metal and Al stresses, has been proposed. Our objective was to investigate (a) the Al-dependent accumulation of endogenous NO and H₂O₂ in root tips and (b) the role of exogenous NO and H₂O₂ in alleviating Al toxicity in root tips and root border cells (RBCs).
Methods Seedlings of soybean (Glycine max) ‘Zhechun No. 3’ were divided into two groups for hydroponic and aeroponic cultured experiments. In order to investigate the response of endogenous NO and H₂O₂ in root tips to 50 μmol·L^-1Al, we determined root elongation, Al content in root apexes, endogenous NO and H₂O₂ content and their location in hydroponic cultured experiments. In the aeroponic culture experiments, seedlings were pretreated with exogenous NO and H₂O₂, then RBCs viability as well as the indicators in hydroponic cultured experiments were tested to clarify the role of exogenous NO and H₂O₂ on alleviating Al toxicity in root tips and RBCs.
Important findings Al inhibited root elongation, increased Al content in root apexes and induced endogenous NO and H₂O₂ accumulation with the hydroponic culture. Results of the aeroponic experiments demonstrated that both 0.25 mmol·L^-1NO donor sodium nitroprusside (SNP) and 0.1 mmol·L^-1 H₂O₂ alleviated the inhibitory effect of Al, decreased Al accumulation in root tips and enhanced RBCs viability. The 0.05 mmol·L^-1 NO scavenger cPTIO (carboxy-PTIO) and 150 U·mL^-1 H₂O₂ scavenger CAT (catalase) reversed the alleviating effect. Furthermore, the results indicated that exogenous NO promoted the accumulation of H₂O₂ in root apexes, while exogenous H₂O₂ did not significantly affect NO content in root apexes. All of these results suggested that the rise of NO and H₂O₂were in accordance with defense response in root apexes and RBCs to Al toxicity in soybean, and the increase of NO may regulate the H₂O₂ production to protect soybean from Al toxicity.

Key words: Al toxicity, hydrogen peroxide, nitric oxide, root apex, root border cells, soybean

王芳妹, 蔡妙珍, 张淑娜, 王宁, 李华飞, 胡雪娜, 虞舒航. NO和H₂O₂诱导大豆根尖和边缘细胞耐铝反应的作用. 植物生态学报, 2011, 35(9): 981-989. DOI: 10.3724/SP.J.1258.2011.00981

WANG Fang-Mei, CAI Miao-Zhen, ZHANG Shu-Na, WANG Ning, LI Hua-Fei, HU Xue-Na, YU Shu-Hang. Effects of nitric oxide and hydrogen peroxide on induction of a defense response in the root tips and root border cells of soybean plants to Al toxicity. Chinese Journal of Plant Ecology, 2011, 35(9): 981-989. DOI: 10.3724/SP.J.1258.2011.00981

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2011.00981

https://www.plant-ecology.com/CN/Y2011/V35/I9/981

图/表 8

图1 不同铝毒害时间对大豆根长的影响(平均值±标准误差)。不同小写字母表示差异显著(p < 0.05)。

Fig. 1 Time-dependent effects of Al toxicity on root elongation of soybean (mean ± SE). Different small letters mean significant differences (p < 0.05).

图2 铝毒对大豆根尖Al含量的影响(平均值±标准误差)。不同小写字母表示差异显著(p < 0.05)。

Fig. 2 Effects of Al toxicity on Al content in soybean root apex (mean ± SE). Different small letters mean significant differences (p < 0.05).

图3 铝毒对大豆根尖内源NO和H2O2含量的影响(平均值±标准误差)。不同小写字母表示差异显著(p < 0.05)。

Fig. 3 Effects of Al toxicity on endogenous NO and H2O2 contents in soybean root apex (mean ± SE). Different small letters mean significant differences (p < 0.05).

图4 铝毒害下大豆根尖NO和H2O2积累的原位观察。 A, B, NO积累的原位观察(A, -Al; B, Al)。C, D, H2O2积累的原位观察(C, -Al; D, Al)。

Fig. 4 The in situ observation of NO and H2O2 accumulation in root apex of soybean under Al toxicity. A, B, the in situ observation of NO accumulation (A, -Al; B, Al). C, D, the in situ observation of H2O2 accumulation (C, -Al; D, Al).

图5 NO和H2O2对铝胁迫下大豆根相对伸长率的影响(平均值±标准误差)。 A, NO。B, H2O2。CAT, 过氧化氢酶; cPTIO, 2-(4-羧基苯)-4,4,5,5-四甲基咪唑-1-O-3-氧化物, 钾盐; SNP, 亚硝基铁氰化钠。不同小写字母表示差异显著(p < 0.05)。

Fig. 5 Effects of NO and H2O2 on relative root elongation in soybean under Al toxicity (mean ± SE). A, NO. B, H2O2. CAT, catalase; cPTIO, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyllimidazoline-1-oxyl-3-oxyde, potassium salt; SNP, sodium nitroprusside. Different small letters mean significant differences (p < 0.05).

图6 NO和H2O2对铝胁迫下大豆根尖Al含量的影响(平均值±标准误差)。 A, NO。B, H2O2。CAT, 过氧化氢酶; cPTIO, 2-(4-羧基苯)-4,4,5,5-四甲基咪唑-1-O-3-氧化物, 钾盐; SNP, 亚硝基铁氰化钠。不同小写字母表示差异显著(p < 0.05)。

Fig. 6 Effects of NO and H2O2 on Al content in root apex of soybean under Al toxicity (mean ± SE). A, NO. B, H2O2. CAT, catalase; cPTIO, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyllimidazoline-1-oxyl-3-oxyde, potassium salt; SNP, sodium nitroprusside. Different small letters mean significant differences (p < 0.05).

图7 Al毒胁迫下NO和H2O2对大豆根尖根边缘细胞活性的影响(平均值±标准误差)。 A, NO。B, H2O2。CAT, 过氧化氢酶; cPTIO, 2-(4-羧基苯)-4,4,5,5-四甲基咪唑-1-O-3-氧化物, 钾盐; SNP, 亚硝基铁氰化钠。不同小写字母表示差异显著(p < 0.05)。

Fig. 7 Effects of NO and H2O2 on root border cells (RBCs) viability in root apex of soybean under Al toxicity(mean ± SE). A, NO. B, H2O2. CAT, catalase; cPTIO, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyllimidazoline-1-oxyl-3-oxyde, potassium salt; SNP, sodium nitroprusside. Different small letters mean significant differences (p < 0.05).

图8 Al毒胁迫下外源NO和H2O2对大豆根尖NO和H2O2含量的影响(平均值±标准误差)。 A, NO。B, H2O2。CAT, 过氧化氢酶; cPTIO, 2-(4-羧基苯)-4,4,5,5-四甲基咪唑-1-O-3-氧化物, 钾盐; SNP, 亚硝基铁氰化钠。不同小写字母表示差异显著(p < 0.05)。

Fig. 8 Effects of exogenous NO and H2O2 contents in root apex of soybean under Al toxicity (mean ± SE). A, NO. B, H2O2. CAT, catalase; cPTIO, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyllimidazoline-1-oxyl-3-oxyde, potassium salt; SNP, sodium nitroprusside. Different small letters mean significant differences (p < 0.05).

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NO和H₂O₂诱导大豆根尖和边缘细胞耐铝反应的作用

Effects of nitric oxide and hydrogen peroxide on induction of a defense response in the root tips and root border cells of soybean plants to Al toxicity

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