大豆根尖边缘细胞和粘液分泌对铝胁迫解除的响应

doi:10.3773/j.issn.1005-264x.2008.05.005

摘要/Abstract

摘要：

以耐铝性明显差异的两个大豆(Glycine max)基因型‘浙秋2号’(耐性)和‘浙春3号’(敏感)为材料, 研究根尖边缘细胞比活度、粘液分泌和根长对铝胁迫和解除胁迫的反应, 明确边缘细胞的粘液分泌对策在铝毒环境中的生态学意义。结果表明, ‘浙秋2号’在100~400 µmol·L^-1Al³⁺处理的3~12 h, 边缘细胞比活率呈递减趋势, 12 h后比活率又略有上升。‘浙春3号’在300和400 µmol·L^-1Al³⁺处理的变化与前者一致。两个大豆基因型的粘液层随着Al³⁺浓度增加和时间延长而增厚, 并于400 µmol·L ^-1Al³⁺处理24 h时达到最大(>17 µm)。‘浙秋2号’在低浓度Al³⁺(100和200 µmol·L ^-1)处理3~6 h后就会分泌大量粘液, ‘浙春3号’则在300 µmol·L ^-1Al³⁺处理12 h后才有类似的变化。‘浙秋2号’在400 µmol·L^-1Al³⁺处理下的根相对伸长率均高于100~300 µmol·L ^-1Al³⁺处理, ‘浙春3号’则表现为Al ³⁺浓度越高, 根伸长受抑越明显。Al³⁺胁迫解除后, ‘浙秋2号’的粘液分泌速度和分泌量急剧下降, ‘浙春3号’在胁迫解除后的24 h, 仍会持续、大量地分泌粘液(>19 µm)。可见, 耐性大豆通过在铝胁迫初期快速、大量地分泌粘液以维持较高的边缘细胞活性和解除胁迫后迅速降低粘液的分泌速度及分泌量来适应铝毒害环境。

关键词: 大豆, 铝毒, 边缘细胞, 粘液

Abstract:

Aims Border cells and their secretory mucilage can form a layer over the surface of younger parts of roots to help form a “rhizosheath” protecting the root tip from biotic and abiotic stresses. Our objective was to investigate the ecological role of mucilage secretion strategies in response to Al exposure and absence.

Methods Soybean (Glycine max) ‘Zheqiu No. 2’ and ‘Zhechun No. 3’ were used in this study. Seedlings were aeroponically cultured in 0.2 mmol·L ^-1CaCl₂ solution, pH 4.5, containing 0, 100, 200, 300 or 400 µmol·L ^-1AlCl₃.Half of the seedlings were harvested after 24 h Al exposure and the other half continued to culture in the absence of Al³⁺. We measured percent viability of border cells, mucilage thickness and relative root elongation.

Important findings Percent viability of root border cells decreased with increasing external Al³⁺concentrations in both cultivars. Percent viability of ‘Zheqiu No. 2’ border cells decreased slightly after a 3-12 h treatment with 100, 200, or 400 µmol·L ^-1Al³⁺and then slightly increased with treatment time, whereas the same change in ‘Zhechun No. 3’ seedlings was noted at Al ³⁺concentrations of 300 and 400 µmol·L ^-1. Al³⁺induced a thicker mucilage layer around border cells of both cultivars, with a maximum of 17 µm after 24 h at 400 µmol·L ^-1Al³⁺. ‘Zheqiu No. 2’ border cells developed a thicker mucilage layer within 3-6 h in response to 100 and 200 µmol·L ^-1Al³⁺, while ‘Zhechun No. 3’ cells exhibited the same change after 12 h to 300 µmol·L ^-1Al³⁺. Relative root elongation of ‘Zheqiu No. 2’ exposed to 400 µmol·L ^-1Al³⁺was significantly higher than to 100-300 µmol·L ^-1Al³⁺treatment, while ‘Zhechun No. 3’ exhibited reduced relative root elongation after exposure to 100-400 µmol·L ^-1Al³⁺. With Al³⁺recovery treatment, ‘Zheqiu No. 2’ border cells developed a thinner layer of mucilage in the absence of Al ³⁺compared with Al³⁺exposure, while ‘Zhechun No. 3’ border cells continued to secrete a conspicuous layer of mucilage. Rapid induction of mucilage production exposure to Al ³⁺stress and abrupt halt by border cells is a specific response to Al³⁺stress and recovery for Al-resistant soybean cultivar.

Key words: soybean, aluminum toxicity, root borer cells, mucilage

蔡妙珍, 邢承华, 刘鹏, 徐根娣, 吴韶辉, 何璠. 大豆根尖边缘细胞和粘液分泌对铝胁迫解除的响应. 植物生态学报, 2008, 32(5): 1007-1014. DOI: 10.3773/j.issn.1005-264x.2008.05.005

CAI Miao-Zhen, XING Cheng-Hua, LIU Peng, XU Gen-Di, WU Shao-Hui, HE Fan. DYNAMIC RESPONSE OF ROOT BORDER CELLS AND THEIR ASSOCIATED MUCILAGE EXUDATION IN SOYBEAN TO Al STRESS AND RECOVERY. Chinese Journal of Plant Ecology, 2008, 32(5): 1007-1014. DOI: 10.3773/j.issn.1005-264x.2008.05.005

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2008.05.005

https://www.plant-ecology.com/CN/Y2008/V32/I5/1007

图/表 5

图1 Al3+对根边缘细胞比活率的影响

Fig. 1 Effect of Al3+on percent viability of root cap border cells of soybean

图2 不同浓度Al3+胁迫下大豆根尖边缘细胞粘液层厚度的动态变化不同字母表示差异达显著水平(p<0.05) Means associated with different letters are significantly different at p<0.05

Fig. 2 Dynamic change of mucilage thickness around detached root cap border cells of soybean exposed to five Al3+levels

图3 不同浓度Al3+胁迫对大豆根相对伸长率的影响

Fig. 3 Effect of five Al3+levels on relative root length of two soybean cultivars

图4 Al3+胁迫解除后大豆根尖边缘细胞粘液层厚度的动态变化图注同图2 Note see Fig. 2

Fig. 4 Dynamic change of mucilage thickness around detached root cap border cells of soybean at Al3+recovery treatment

图5 Al3+胁迫解除后大豆根相对伸长率的动态变化

Fig. 5 Effect of Al3+recovery on relative root length of two soybean cultivars

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