外源亚精胺对根际低氧胁迫下黄瓜幼苗根系多胺含量和抗氧化酶活性的影响

doi:10.17521/cjpe.2006.0017

摘要/Abstract

摘要：

采用营养液水培,研究了根际低氧胁迫下外源亚精胺对两个抗低氧能力不同的黄瓜(Cucumis sativus)品种(‘中农8号’和‘绿霸春4号’)根系中多胺含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响。结果表明,外源亚精胺能显著提高低氧胁迫下黄瓜幼苗根系中亚精胺和精胺含量,降低腐胺含量,同时,根系中SOD、POD、CAT活性也相应提高,使得幼苗鲜重和干重明显增加;单纯低氧处理及外源亚精胺的加入,抗低氧能力较强的品种‘绿霸春4号’较抗低氧能力较弱的品种‘中农8号’根系中抗氧化酶活性高。黄瓜幼苗根系中较高的亚精胺、精胺含量和较低的腐胺含量可能有利于提高抗氧化酶活性,增强幼苗的低氧逆境适应能力。

关键词: 多胺, 抗氧化酶, 低氧胁迫, 黄瓜

Abstract:

Polyamines (PAs) have long been recognized to be linked with growth, differentiation, plant senescence and defense reactions of plants to various environmental stresses. However, little is known about their physiological relevance in plants subjected to hypoxia stress. In the present experiment, the effects of exogenous spermidine on polyamine content and antioxidant enzyme activities in seedlings of two cucumber cultivars (Cucumis sativus cv. Zhongnong No.8 and cv. Lübachun No.4) with different resistances to hypoxia stress were investigated. The results showed that under hypoxia treatment, the contents of putrescine (Put), spermidine (Spd), and spermine (Spm) in roots of seedlings of two cucumber cultivars increased significantly in the early period of treatment and then decreased in the later period. Over the entire treatment period, concentrations of PAs were significantly higher than those in the control. Activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) over the treatment period followed the same pattern as PAs. The fresh weight and dry weight of the two cucumber cultivar seedlings were significantly lower than the controls. Exogenous Spd treatment increased the contents of Spd and Spm in roots while decreased the Put content when compared to the hypoxia treatment, enhanced the activities of the antioxidant enzymes, and the fresh and dry weight of seedlings were similar to the controls. Under the same treatment, the activities of the antioxidant enzymes in the roots of the hypoxia-resistant cultivar (cv. Lübachun No.4) were higher than in the hypoxia-sensitive cultivar (cv. Zhongnong No.8). These results indicated that exogenous Spd can change the contents of PAs in the roots of both cucumber cultivars under hypoxia stress, and higher levels of Spd and Spm in roots can enhance both the activities of antioxidant enzymes and the growth of cucumber cultivar seedlings.

Key words: Polyamines, Antioxidant enzyme, Hypoxia stress, Cucumber (Cucumis sativus)

李璟, 胡晓辉, 郭世荣, 王素平, 王鸣华. 外源亚精胺对根际低氧胁迫下黄瓜幼苗根系多胺含量和抗氧化酶活性的影响. 植物生态学报, 2006, 30(1): 118-123. DOI: 10.17521/cjpe.2006.0017

LI Jing, HU Xiao-Hui, GUO Shi-Rong, WANG Su-Ping, WANG Ming-Hua. EFFECT OF EXOGENOUS SPERMIDINE ON POLYAMINE CONTENT AND ANTIOXIDANT ENZYME ACTIVITIES IN ROOTS OF CUCUMBER SEEDLINGS UNDER ROOT-ZONE HYPOXIA STRESS. Chinese Journal of Plant Ecology, 2006, 30(1): 118-123. DOI: 10.17521/cjpe.2006.0017

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2006.0017

https://www.plant-ecology.com/CN/Y2006/V30/I1/118

图/表 3

表1 外源Spd对低氧胁迫下黄瓜植株鲜重和干重的影响

Table 1 Effect of exogenous Spd on fresh weight and dry weight of cucumber seedlings under hypoxia stress

处理 Treatment	鲜重 Fresh weight (g·plant^-1)		干重 Dry weight(g·plant^-1)
处理 Treatment	‘中农八号’ ‘Zhongnong No.8’	‘绿霸春4号’ ‘Lübachun No.4’	‘中农8号’ ‘Zhongnong No.8’	‘绿霸春4号’ ‘Lübachun No.4’
对照 Control	27.34±0.80^a	29.43±0.98^a	1.71±0.07^a	1.84±0.03^a
低氧胁迫 Hypoxia stress	18.08±0.92^c	21.47±0.57^b	1.28±0.03^b	1.44±0.04^b
低氧+Spd Hypoxia+Spd	24.73±0.81^b	27.59±0.41^a	1.59±0.04^a	1.76±0.04^a

图1 根际低氧胁迫下外源亚精胺对黄瓜根系多胺含量的影响

Fig.1 Effect of exogenous Spd on PAs content of roots in cucumber seedlings under hypoxia stress

图2 低氧胁迫下外源亚精胺对黄瓜幼苗根系抗氧化酶活性的影响图例同图1

Fig.2 Effect of exogenous Spd on antioxidant enzyme activity in roots of cucumber seedlings under hypoxia stress Legends see Fig.1

参考文献 17

[1]	Borrell A, Carbonell L, Farràs R, Puig-Parellad P, Tiburcio AF (1997). Polyamines inhibit lipid peroxidation in senescing oat leaves. Physiologia Plantarum, 99,385-390.
[2]	Bouchereau A, Aziz A, Larher F, Martin-Tanguy J (1999). Polyamines and environmental challenges: recent developments. Plant Science, 140,103-125.
[3]	Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981). Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32,93-101.
[4]	Guo SR, Tachibana SJ (1997). Effect of dissolved O₂ levels in a nutrient of tomato and mineral nutrition of tomato and cucumber seedlings. Journal of the Japanese Society for Horticultural Science, 66,331-337.
[5]	Hummel I, Couee I, ElAmrani A, Martin-Tanguy J, Hennion F (2002). Involvement of polyamines in root development at low temperature in the subantarctic cruciferous species Pringlea antiscorbutica. Journal of Experimental Botany, 53,1463-1473. URL PMID
[6]	Lee MM, Lee SH, Park KY (1997). Effects of spermine on ethylene biosynthesis in cut carnation ( Dianthus caryophyllus L.) flowers during senescence. Journal of Plant Physiology, 151,68-73.
[7]	Li HS (李合生) (2000). Principles and Techniques of Plant Physiological Biochemical Experiment (植物生理生化实验原理和技术). Higher Education Press, Beijing,167-169. (in Chinese)
[8]	Liu HP, Dong BH, Zhang YY, Liu ZP, Liu YL (2004). Relationship between osmotic stress and the levels of free, conjugated and bound polyamines in leaves of wheat seedlings. Plant Science, 166,1261-1267.
[9]	Liu J (刘俊), Liu YL (刘友良) (2004). The relations between polyamine types and forms and polyamine oxidase activities in barley seedlings under salt stress. Journal of Plant Physiology and Molecular Biology (植物生理与分子生物学学报), 30,141-146. (in Chinese with English abstract)
[10]	Ma YH (马月花), Guo SR (郭世荣) (2004). Evaluation of tolerance to root-zone hypoxia stress of different cucumber varieties. Jiangsu Agricultural Sciences (江苏农业科学), (5),68-70. (in Chinese)
[11]	Ren HX (任红旭), Chen X (陈雄), Wang YF (王亚馥) (2001). Changes in antioxidative enzymes and polyamines in wheat seedlings with different drought resistance under drought and salt stress. Acta Phytoecologica Sinica (植物生态学报), 25,709-715. (in Chinese with English abstract)
[12]	Tadolini B (1988). Polyamine inhibition of lipoperoxidation: the influence of polyamines on iron oxidation in the presence of compounds mimicking phospholipid polar heads. Biochemical Journal, 249,33-36.
[13]	Tiburcio AF, Campos JL, Figueras X, Besford RT (1993). Recent advances in the understanding of polyamines functions during plant development. Plant Growth Regulation, 12,331-340.
[14]	Ushimaru T, Kanematsu S, Katayama M (2001). Antioxidative enzymes in seedings of Nelumbo nucifera germinated under water. Physiologia Plantarum, 112,39-46. DOI URL PMID
[15]	van Toai TT, Bolles CS (1991). Postanoxic injury in soybean ( Glycine max) seedlings. Plant Physiology, 97,588-592. DOI URL PMID
[16]	Xu YC (徐仰仓), Wang J (王静), Liu H (刘华), Wang GX (王根轩) (2001). Promoting effect of exogenous spermine on anti-oxidative enzyme activity in wheat seedlings. Acta Phytophysiologica Sinica (植物生理学报), 27,349-352. (in Chinese with English abstract)
[17]	Zeng SX (曾韶西), Wang YR (王以柔), Li MR (李美如) (1997). Comparison of the changes of membrane protective system in rice seedlings during enhancement of chilling resistance by different stress pretreatment. Acta Botanica Sinica (植物学报), 39,308-314. (in Chinese with English abstract)