植物光合CO2响应模型对光下(暗)呼吸速率拟合的探讨

doi:10.3724/SP.J.1258.2014.00130

植物生态学报 ›› 2014, Vol. 38 ›› Issue (12): 1356-1363.DOI: 10.3724/SP.J.1258.2014.00130 cstr: 32100.14.SP.J.1258.2014.00130

植物光合CO₂响应模型对光下(暗)呼吸速率拟合的探讨

康华靖¹^,²^,³^,⁴, 陶月良⁵, 权伟⁴, 王伟⁴, 欧阳竹²^,³^,⁴^,^*()

¹温州科技职业学院, 浙江温州 325006
²中国科学院地理科学与资源研究所生态网络观测与模拟重点实验室, 北京 100101
³中国科学院禹城综合试验站, 北京 100101
⁴中国科学院大学, 北京 100049
⁵温州大学生命与环境科学学院, 浙江温州 325035

收稿日期:2014-03-21 接受日期:2014-05-10 出版日期:2014-03-21 发布日期:2015-04-16
作者简介:^*(E-mail: ouyz@igsnrr.ac.cn)
基金资助:
国家高技术研究发展计划(863计划)——作物健康生长农艺调控技术(2013AA102903);中国科学院地理科学与资源研究所“一三五”战略科技计划项目(2012ZD004);浙江省教育厅项目(Y201327619)

Fitting mitochondrial respiration rates under light by photosynthetic CO₂ response models

KANG Hua-Jing¹^,²^,³^,⁴, TAO Yue-Liang⁵, QUAN Wei⁴, WANG Wei⁴, OUYANG Zhu²^,³^,⁴^,^*()

¹Wenzhou Vocational & Technical College, Wenzhou, Zhejiang 325026, China;
²Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
³Yucheng Comprehensive Experiment Station, China Academy of Sciences, Beijing 100101, China
⁴University of Chinese Academy of Sciences, Beijing 100049, China
⁵College of Life & Environmental Science, Wenzhou University, Wenzhou, Zhejiang 325035, China;

Received:2014-03-21 Accepted:2014-05-10 Online:2014-03-21 Published:2015-04-16

摘要/Abstract

摘要：

运用LI-6400便携式光合作用系统测定了不同光强(2000、1500、1000和500 μmol·m^-2·s^-1)和两种O₂浓度(21%和2%的O₂)下冬小麦(Triticum aestivum)灌浆期旗叶的CO₂响应曲线, 比较了现有CO₂响应模型(生化模型、直角双曲线模型和直角双曲线修正模型)拟合给出光下(暗)呼吸与测量值之间的差异。结果显示, 直角双曲线修正模型所给出的光下呼吸速率拟合值与测量值最为接近。植物光合作用对大气CO₂响应(A/C_a)的拟合结果优于光合作用对胞间CO₂浓度(A/C_i)的拟合。然而, 所有模型基于A/C_a拟合的光下(暗)呼吸在整体上与测量值存在显著差异(p < 0.05), 推测与现有模型没有考虑CO₂浓度对光呼吸和光下暗呼吸速率的影响有关。对小麦的试验结果表明, CO₂浓度对光呼吸和光下暗呼吸均有显著影响: 随着CO₂浓度的增加(0-1400 μmol·mol^-1), 不同光强下的表观光呼吸变化范围分别为5.035-11.670、4.222-11.650、4.330-10.999和3.263-9.094 μmol CO_2·m^-2·s^-1; 光下暗呼吸的变化范围分别为0.491-2.987、0.457-2.955、0.545-3.139和0.448-3.139 μmol CO₂·m^-2·s^-1。回归分析发现, 表观光呼吸和光下暗呼吸与CO₂浓度之间均存在较好的相关性。然而, 将该回归关系整合到现有模型中, 是否会优化模型, 从而提高模型对相关光合参数估算的准确性尚有待于进一步研究。

关键词: CO₂浓度, 光下暗呼吸, 模型, 光呼吸, 小麦

Abstract:

Aims The objective of this study was to compare the values of respiration under light derived by fitting a photosynthetic CO₂ response model and measurements, in order to provide information for model optimization.

Methods Using combined gas exchange measurements and a low O₂ (2% O₂) method, the responses of photosynthetic rate (P_n) to CO₂ at different light intensities (2000, 1500, 1000 and 500 μmol·m^-2·s^-1) in the flag leaves of wheat were measured. The measured data were fitted by a biochemical model, a rectangular hyperbola model and a modified rectangular hyperbola model of the photosynthetic response to intercellular CO₂ concentration (A/C_i) and air CO₂ concentration (A/C_a), aiming to approach the reasonability of the fitted results obtained from the models.

Important findings The sequence of fitting effect of the three CO₂ response models in descending order was as follows: modified rectangular hyperbola model > rectangular hyperbola model > biochemical model. Fitted values of A/C_a curve was more reasonable than A/C_i curve, because the photorespiration and mitochondrial respiration under light (R_d) estimated by the former better matched the measured values. However, there were significant differences in the whole between the fitted and measured values. The reason could be that the effect of CO₂concentrations on R_d and apparent photorespiration (R_pa) is neglected in all the current CO₂ response models. Our results showed that CO₂ concentration had a marked effect on R_pa and R_d. With increasing CO₂ concentration, R_pa and R_d increased first, and then decreased sharply. Take 2000 μmol·m^-2·s^-1 for example, R_pa varied between 5.035 and 11.670 μmol CO₂·m^-2·s^-1, and R_d varied between 0.491 and 2.987 μmol CO₂·m^-2·s^-1. Regression analyses indicated that R_pa and R_dwere well related to CO₂ concentrations at different light intensities.

Key words: CO₂concentration, mitochondrial respiration under light, model, photorespiration, wheat

康华靖, 陶月良, 权伟, 王伟, 欧阳竹. 植物光合CO₂响应模型对光下(暗)呼吸速率拟合的探讨. 植物生态学报, 2014, 38(12): 1356-1363. DOI: 10.3724/SP.J.1258.2014.00130

KANG Hua-Jing, TAO Yue-Liang, QUAN Wei, WANG Wei, OUYANG Zhu. Fitting mitochondrial respiration rates under light by photosynthetic CO₂ response models. Chinese Journal of Plant Ecology, 2014, 38(12): 1356-1363. DOI: 10.3724/SP.J.1258.2014.00130

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2014.00130

https://www.plant-ecology.com/CN/Y2014/V38/I12/1356

图/表 7

图1 不同光合有效辐射(PAR)下小麦旗叶表观光呼吸速率(Rpa)对CO2浓度(Ca)的响应(平均值±标准偏差)。

Fig. 1 Apparent photorespiration rate (Rpa) in response to CO2 concentration (Ca) at different photosynthetically active radiation (PAR) in flag leaves of wheat (mean ± SD).

图2 不同光合有效辐射(PAR)下小麦叶片光呼吸CO2回收利用率(Re-i)或被抑制率(Ii)对CO2浓度(Ca)的响应(平均值±标准偏差)。

Fig. 2 Recovery (Re-i) or inhibition (Ii) of photorespiratory CO2 in response to CO2 concentration (Ca) at different photosynthetically active radiation (PAR) (mean ± SD).

图3 21%和2%的O2浓度下小麦旗叶暗呼吸(Rn)对CO2浓度(Ca)的响应(平均值±标准偏差)。

图4 不同CO2浓度(Ca)下小麦旗叶的光下暗呼吸速率(Rd) (平均值±标准偏差)。

Fig. 4 Mitochondrial respiration under light (Rd) in response to different CO2 concentration (Ca) in flag leaves of wheat (mean ± SD).

表1 不同光合有效辐射(PAR)下的小麦旗叶在CO2浓度为0时表观光合速率的测量值(μmol CO2·m-2·s-1) (平均值±标准偏差)

Table 1 Measured values of photosynthetic rate in flag leaves of wheat at different photosynthetically active radiation (PAR) when CO2 concentration was 0 (μmol CO2·m-2·s-1) (mean ± SD)

	PAR (μmol·m^-2·s^-1)
	2 000	1 500	1 000	500
光下呼吸速率 P_n0-21%	6.843 ± 0.341^a	6.343 ± 0.762^a	6.536 ± 0.408^a	5.729 ± 0.499^a
光下暗呼吸速率 P_n0-2%	1.934 ± 0.190^c	2.064 ± 0.091^c	2.164 ± 0.112^c	2.539 ± 0.285^c
表观光呼吸 R_pa0	5.035 ± 0.194^b	4.222 ± 0.832^b	4.330 ± 0.394^b	3.263 ± 0.544^b

表2 不同光合有效辐射(PAR)下小麦旗叶的光下(暗)呼吸速率拟合值(21%的O2) (平均值±标准偏差)

Table 2 Fitted values of mitochondrial respiration in flag leaves of wheat under light at different photosynthetically active radiation (PAR) and 21% O2 (mean ± SD)

		PAR (μmol·m^-2·s^-1)
		2 000	1 500	1 000	500
A-C_i	A	21.667 ± 0.577^*#	21.857 ± 0.378^*#	21.750 ± 0.500^*#	20.850 ± 1.226^*#
	B	17.924 ± 1.250^*#	16.947 ± 0.908^*#	15.754 ± 1.200^*#	12.780 ± 0.905^*#
	C	14.809 ± 0.279^*#	13.745 ± 1.117^*#	13.412 ± 0.744^*#	11.655 ± 1.154^*#
A-C_a	A	21.667 ± 0.577^*#	21.857 ± 0.378^*#	21.750 ± 0.500^*#	20.850 ± 1.226^*#
	B	8.745 ± 1.340^*#	8.335 ± 0.695^*#	8.769 ± 0.931^*#	7.245 ± 0.589^*#
	C	7.743 ± 0.556^*#	6.907 ± 0.528^*	7.579 ± 0.570^*#	6.491 ± 0.616^*#

表3 不同光合有效辐射(PAR)下小麦旗叶的光下暗呼吸速率拟合值(2%的O2) (平均值±标准偏差)

Table 3 Fitted values of mitochondrial respiration in flag leaves of wheat under light at different photosynthetically active radiation (PAR) and 2% O2 (mean ± SD)

		PAR (μmol·m^-2·s^-1)
		2 000	1 500	1 000	500
A-C_i	A	12.067 ± 0.808^*	12.286 ± 0.445^*	12.400 ± 0.783^*	11.350 ± 0.823^*
	B	8.293 ± 1.500^*	9.245 ± 1.093^*	9.320 ± 0.843^*	9.416 ± 1.475^*
	C	6.099 ± 0.730^*	6.507 ± 0.913^*	6.609 ± 0.515^*	7.264 ± 0.561^*
A-C_a	A	21.667 ± 0.577^*	21.857 ± 0.378^*	21.750 ± 0.500^*	20.850 ± 1.226^*
	B	4.520 ± 1.118^*	5.022 ± 1.306^*	5.345 ± 0.828^*	4.919 ± 0.244^*
	C	2.766 ± 0.564^*	3.323 ± 0.977^*	3.326 ± 0.554^*	3.798 ± 0.251^*

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植物光合CO₂响应模型对光下(暗)呼吸速率拟合的探讨

Fitting mitochondrial respiration rates under light by photosynthetic CO₂ response models

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