植物生态学报 ›› 2010, Vol. 34 ›› Issue (10): 1196-1203.DOI: 10.3773/j.issn.1005-264x.2010.10.008

• 研究论文 • 上一篇    下一篇

高大气CO2浓度下氮素对小麦叶片光能利用的影响

张绪成1,2,3, 于显枫1, 高世铭1,*()   

  1. 1甘肃省农业科学院农业部西北作物抗旱栽培与耕作重点开放实验室, 兰州 730070
    2中国农业大学资源环境学院, 北京 100094
    3中国科学院水土保持研究所, 黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨凌 712100
  • 收稿日期:2009-11-09 接受日期:2010-01-07 出版日期:2010-11-09 发布日期:2010-10-31
  • 通讯作者: 高世铭
  • 作者简介:* E-mail: gszhangxuch@163.com

Effects of nitrogen application rates on photosynthetic energy utilization in wheat leaves under elevated atmospheric CO2 concentration

ZHANG Xu-Cheng1,2,3, YU Xian-Feng1, GAO Shi-Ming1,*()   

  1. 1Key Laboratory of Northwest Crop Drought-Resistant Farming, Ministry of Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China
    2College of Resources and Environment, China Agricultural University, Beijing 100094, China
    3State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences, Yangling, Shaanxi 712100, China
  • Received:2009-11-09 Accepted:2010-01-07 Online:2010-11-09 Published:2010-10-31
  • Contact: GAO Shi-Ming

摘要:

关于氮素对高大气CO2浓度下C3植物光合作用适应现象的调节机理已有较为深入的研究, 但对其光合作用适应现象的光合能量转化和分配机制缺乏系统分析。该文以大气CO2浓度和施氮量为处理手段, 通过测定小麦(Triticum aestivum)抽穗期叶片的光合作用-胞间CO2浓度响应曲线以及荧光动力学参数来测算光合电子传递速率和分配去向, 研究了长期高大气CO2浓度下小麦叶片光合电子传递和分配对施氮量的响应。结果表明, 与正常大气CO2浓度处理相比, 高大气CO2浓度下小麦叶片较多的激发能以热量的形式耗散, 增施氮素可使更多的激发能向光化学反应方向的分配, 降低光合能量的热耗散速率; 大气CO2浓度升高后小麦叶片光化学淬灭系数无明显变化, 高氮叶片的非光化学猝灭降低而低氮叶片明显升高, 施氮促进PSII反应中心的开放比例, 降低光能的热耗散; 高大气CO2浓度下高氮叶片通过PSII反应中心的光合电子传递速率(JF)较高, 而且参与光呼吸的非环式电子流速率(J0)显著降低, 较正常大气CO2浓度处理的高氮叶片下降了88.40%, 光合速率增加46.47%; 高大气CO2浓度下小麦叶片JF-J0升高而J0/JF显著下降, 光呼吸耗能被抑制, 更多的光合电子分配至光合还原过程。因此, 大气CO2浓度增高条件下, 小麦叶片激发能的热耗散速率增加, 但增施氮素后小麦叶片PSII反应中心开放比例提高, 光化学速率增加, 进入PSII反应中心的电子流速率明显升高, 光呼吸作用被抑制, 光合电子较多地进入光化学过程, 这可能是高氮条件下光合作用适应性下调被缓解的一个原因。

关键词: 大气CO2浓度增高, 氮素, 光合电子传递速率, 光能分配, 小麦

Abstract:

Aims It is well documented that the increment of nitrogen application rates leads to the release of photosynthesis acclimation of C3 plants under elevated atmospheric CO2 concentration. However, current knowledge of the effects of nitrogen application rates on photosynthetic electron transport and distribution and its potential influence on photosynthesis acclimation is inadequate. The objective of our potted-experiment was to study the effect of nitrogen application rates on photosynthetic electronic transports and distribution in wheat leaves under elevated atmospheric CO2 concentration.

Methods Using open-top chambers in simulating elevated atmospheric CO2 concentration, we grew wheat (Triticum aestivum) under different nitrogen application rates and atmospheric CO2 concentrations. We estimated the photosynthetic electron transport rate and its distribution by measurement of photosynthetic rate (Pn)-intercellular CO2 concentration (Ci) curves and chlorophyll fluorescence parameters of wheat leaves in the heading stage.

Important findings Compared with ambient atmospheric CO2 concentration treatments, more light energy excited by antenna pigments was dissipated as heat in wheat leaves under elevated atmospheric CO2 concentration, the photochemical rate was increased and heat dissipative rate was decreased significantly in high-N wheat leaves. The non-photochemical quenching was decreased in high-N leaves but increased in low-N leaves, photochemical quenching coefficient was not changed significantly in both high-N and low-N leaves and the opening ratio of PSII reaction center would be increased and heat dissipation would be decreased where N was sufficient under elevated atmospheric CO2 concentration. The photosynthetic electron rate of PSII (JF) was increased, noncyclic electron transport rate involved in photorespiration (J0) in high-N wheat leaves was decreased by 88.40% and JF-J0 increased and J0/JF decreased significantly under elevated atmospheric CO2 concentration. Therefore, photorespiration was inhibited and more photosynthetic electrons were transported to photochemical process in wheat leaves under elevated atmospheric CO2 concentration and the Pn was increased by 46.47%. We concluded that although more excited light energy of wheat leaves would be dissipated as heat under elevated atmospheric CO2 concentration, the opening ratio of PSII reaction center, photochemical rates and JF increased, J0 decreased where nitrogen was applied sufficiently and more photosynthetic energy was transported to photochemical process. This may be a reason why photosynthesis acclimation of C3 plant is released in higher nitrogen content soil under elevated atmospheric CO2 concentration.

Key words: atmospheric CO2 enrichment, nitrogen application rates, photosynthetic electron transport rate, photosynthetic energy distribution, wheat