EFFECTS OF SEAWATER TREATMENTS ON THE PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF HELIANTHUS TUBEROSUS SEEDLINGS AND RESPONSE TO PHOSPHORUS SUPPLY

doi:10.17521/cjpe.2006.0041

Abstract

Abstract:

Growing plants that are tolerant to salt_water is one option for exploiting mudflats along the coast. Pot experiments were carried out to study the effects of seawater on growth, inorganic and organic osmotic, antioxidant enzyme, membrane permeability in Helianthus tuberosus seedlings and response to phosphorus supply. The results showed that 1) compared with the control, the fresh weights of roots and aerial parts of Helianthus tuberosus seedlings treated with 10% seawater changed slightly, even were higher than the control, whereas decreased with treatment of 25% seawaters. The fresh weights of roots and aerial parts increased with the concentration of phosphorus augment. The trends of dry weights of roots and aerial parts resembled the trends of fresh weights with the same treatments. 2) The contents of proline and soluble_sugar increased obviously with increasing seawater concentrations. The contents of proline and soluble_sugar increased on 8 days and decreased on 12 days after the treatments of 10% and 25% seawater. They both increased with phosphorus augment. 3) The activities of SOD, POD and CAT in leaves of seawater_stressed plants were significantly stimulated compared to control plants, and the trends toned up with increasing seawater concentrations. The activities of SOD, POD and CAT all significantly stimulated with phosphorus augment. 4) Compared with the control, there was slight change of MDA content in leaves of Helianthus tuberosus seedlings treated with 10% seawater. The MDA content and ELP significantly increased with the treatment of 25% seawater. They both decreased with phosphorus augment. 5) The Na⁺ and Cl^- contents of the aerial parts and roots significantly increased with increasing seawater concentrations and time lasting. And the contents of Na⁺ and Cl^- decreased with phosphorus augment. Compared with the control, the contents of K⁺, Ca²⁺ and Mg²⁺ of the aerial parts and roots increased, and they all increased with phosphorus augment. Above all, phosphorus can improve nutrition and increase resisting salinity for Helianthus tuberosus seedlings.

Key words: Helianthus tuberosus, Effect of phosphorus, Treatment of seawater, Membrane permeability, Antioxidant enzyme, Ions

LONG Xiao_Hua, LIU Zhao_Pu, XU Wen_Jun. EFFECTS OF SEAWATER TREATMENTS ON THE PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF HELIANTHUS TUBEROSUS SEEDLINGS AND RESPONSE TO PHOSPHORUS SUPPLY[J]. Chin J Plant Ecol, 2006, 30(2): 307-313.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2006.0041

https://www.plant-ecology.com/EN/Y2006/V30/I2/307

Figures/Tables 6

Table 1 Basic ion contents of Laizhou bay seawater

pH	阴离子 Anion(g·L^-1)				阳离子 Cation(g·L^-1)				含盐量 Salinity(g·L^-1)
pH	C $O 3 2 -$	HC $O 3 -$	Cl^-	S $O 4 2 -$	Ca²⁺	Mg²⁺	K⁺	Na⁺	含盐量 Salinity(g·L^-1)
8.30	-	0.132	17.52	3.867	0.785	1.027	0.596	9.480	33.33

Table 1 Basic ion contents of Laizhou bay seawater

pH	阴离子 Anion(g·L^-1)				阳离子 Cation(g·L^-1)				含盐量 Salinity(g·L^-1)
pH	C $O 3 2 -$	HC $O 3 -$	Cl^-	S $O 4 2 -$	Ca²⁺	Mg²⁺	K⁺	Na⁺	含盐量 Salinity(g·L^-1)
8.30	-	0.132	17.52	3.867	0.785	1.027	0.596	9.480	33.33

Table 2 Effects of different treatment on biomass fresh weight and dry weight of shoot and root of Helianthus tuberosus seedlings

处理 Treatment	天数 Days	S₀		S₁		S₁P		S₂		S₂P
处理 Treatment	天数 Days	地上部 Shoot	根 Root	地上部 Shoot	根 Root	地上部 Shoot	根 Root	地上部 Shoot	根 Root	地上部 Shoot	根 Root
鲜重 Fresh weight (g·plant^-1)	4	3.48^b	3.30^c	3.56^b	3.54^b	3.84^a	3.67^a	3.36^c	3.65^a	3.48^b	3.73^a
	8	4.26^cd	4.35^cd	4.58^b	4.68^b	4.67^a	4.85^a	4.18^d	4.23^d	4.32^c	4.46^c
	12	5.58^a	5.03^ab	5.59^a	4.96^b	5.68^a	5.12^a	4.59^c	4.37^d	4.73^b	4.62^c
干重 Dry weight (g·plant^-1)	4	0.42^b	0.19^c	0.43^ab	0.20^bc	0.45^a	0.21^ab	0.38^d	0.21^a	0.39^cd	0.22^a
	8	0.49^b	0.25^b	0.53^a	0.26^b	0.54^a	0.28^a	0.46^c	0.25^b	0.48^bc	0.27^ab
	12	0.59^a	0.33^a	0.58^a	0.32^a	0.59^a	0.33^a	0.53^b	0.30^b	0.55^b	0.32^a

Table 3 Effects of different treatment on proline and soluble_suger content in leaf of Helianthus tuberosus seedlings

处理Treatment	天数Days	S₀	S₁	S₁P	S₂	S₂P
脯氨酸 Proline (μg·g^-1 FW)	4	17.12^cd	15.92^d	24.21^b	17.38^c	41.72^a
	8	8.97^e	16.95^d	41.64^c	281.23^b	431.08^a
	12	5.20^e	14.37^d	23.73^c	118.91^b	233.68^a
可溶性糖 Soluble_suger(%)	4	0.55^d	0.74^c	0.89^bc	0.98^b	1.17^a
	8	0.87^e	1.65^d	2.93^b	2.17^c	3.32^a
	12	0.90^e	1.01^de	1.16^cd	1.57^b	1.91^a

Table 4 Effects of different treatment on SOD, POD and CAT activity in leaf of Helianthus tuberosus seedlings

处理Treatment	天数Days	S₀	S₁	S₁P	S₂	S₂P
SOD (U·mg^-1 protein)	4	3.88^b	3.37^d	3.68^c	3.78^bc	4.26^a
	8	3.98^d	3.62^e	4.15^cd	5.05^b	5.66^a
	12	4.28^e	4.99^d	5.73^c	6.11^b	7.94^a
POD (mg^-1 protein·min^-1)	4	0.023^d	0.046^c	0.058^b	0.062^b	0.088^a
	8	0.304^d	0.400^c	0.496^a	0.393^c	0.432^b
	12	0.316^d	0.337^d	0.427^a	0.359^c	0.402^b
CAT (mg^-1 protein·min^-1)	4	0.080^b	0.062^c	0.084^b	0.075^bc	0.147^a
	8	0.152^c	0.116^d	0.186^b	0.198^b	0.262^a
	12	0.236^d	0.366^c	0.490^b	0.469^b	0.602^a

Table 5 Effects of different treatment on MDA content and electrolytic leakage percentage (ELP) in leaf of Helianthus tuberosus seedlings

处理Treatment	天数Days	S₀	S₁	S₁P	S₂	S₂P
MDA (nmol·g^-1 FW)	4	4.72^c	4.82^b	4.84^b	5.38^a	4.85^b
	8	5.77^c	5.38^de	5.15^e	9.19^a	8.10^b
	12	3.70^de	4.91^c	3.39^e	7.53^a	5.77^b
膜透性 ELP(%)	4	4.99^bc	5.03^b	4.87^cd	5.24^a	4.74^d
	8	5.08^d	5.31^bc	5.15^cd	5.88^a	5.32^b
	12	5.20^d	5.47^c	5.17^d	6.74^a	6.03^b

Table 6 Effects of different treatment on K+、Na+、Ca2+、Mg2+ and Cl- content in shoot and root of Helianthus tuberosus seedlings

处理 Treatment	天数 Days	S₀		S₁		S₁P		S₂		S₂P
处理 Treatment	天数 Days	地上部 Shoot	根 Root	地上部 Shoot	根 Root	地上部 Shoot	根 Root	地上部 Shoot	根 Root	地上部 Shoot	根 Root
K⁺ (mmol· g^-1 DW)	4	1.24^c	1.16^d	1.44^b	1.61^c	1.43^b	1.94^a	1.45^b	1.65^c	1.61^a	1.77^b
	8	1.12^c	1.29^c	1.10^c	1.32^c	1.20^b	1.48^b	1.14^c	1.45^b	1.38^a	1.64^a
	12	1.04^c	1.20^c	1.05^c	1.41^b	1.18^b	1.52^a	1.21^b	1.40^b	1.49^a	1.47^ab
Na⁺ (mmol· g^-1 DW)	4	0.05^d	0.16^d	0.17^c	0.73^b	0.16^c	0.52^c	0.71^a	1.07^a	0.53^b	0.79^b
	8	0.06^e	0.31^d	0.41^c	1.13^b	0.28^d	0.91^c	1.05^a	1.32^a	0.61^b	1.05^bc
	12	0.07^d	0.28^e	0.55^c	1.48^bc	0.44^c	1.17^d	1.29^a	1.99^a	1.04^b	1.35^c
Ca²⁺ (mmol· g^-1 DW)	4	0.13^b	0.13^c	0.14^b	0.14^bc	0.13^b	0.15^b	0.13^b	0.14^bc	0.17^a	0.18^a
	8	0.13^d	0.12^d	0.15^cd	0.13^d	0.17^bc	0.17^bc	0.18^b	0.16^c	0.23^a	0.19^a
	12	0.14^d	0.15^d	0.18^c	0.18^c	0.21^b	0.20^b	0.22^b	0.19^bc	0.30^a	0.23^a
Mg²⁺ (mmol· g^-1 DW)	4	0.10^e	0.23^d	0.15^d	0.24^d	0.20^c	0.33^b	0.23^b	0.27^c	0.26^a	0.37^a
	8	0.18^e	0.31^d	0.19^de	0.33^cd	0.21^cd	0.39^b	0.27^b	0.37^b	0.32^a	0.46^a
	12	0.20^d	0.35^e	0.22^cd	0.41^d	0.25^c	0.45^c	0.41^b	0.49^b	0.47^a	0.54^a
Cl^- (mmol· g^-1 DW)	4	0.21^e	0.27^e	0.87^c	1.06^cd	0.74^d	0.96^d	1.19^a	1.66^a	1.01^b	1.29^b
	8	0.26^e	0.37^e	1.16^c	1.70^bc	0.88^d	1.26^d	1.93^a	2.02^a	1.53^b	1.54^c
	12	0.27^e	0.31^e	1.33^c	2.06^b	1.02^d	1.58^d	2.06^a	2.26^a	1.65^b	1.79^c

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