POLLEN VIABILITY AND VARIATION IN CATHAYA ARGYROPHYLLA

doi:10.17521/cjpe.2007.0149

Abstract

Abstract:

Aims Cathaya argyrophylla is one of the most endangered conifers endemic to China. Previous studies showed that this species was characterized by unusually low fertility and high embryonic mortality, which may be factors leading to its endangerment. Because knowledge of pollen viability is important for successful reproduction of plant species, we investigated pollen viability in natural populations of C. argyrophylla.

Methods We collected pollen samples from 16 individuals of seven populations in two locations, Dayaoshan (DYS) in Sichuan and Huaping (HP) in Guangxi. We evaluated pollen viability using the TTC method and analyzed its variation based on ANOVA.

Important findings Pollen viability of C. argyrophylla is comparable to other gymnosperms. Viability was stable in dry and low temperature conditions, and the optional sucrose concentration for pollen germination was about 13%. We found low variation of pollen viability among individuals within populations (p>0.05) but significant differentiation between populations within regions and among regions (p<0.05). The pollen viability ofC. argyrophylla is normal and thus not likely relevant to its endangerment. We proposed artificial crossings between genetically distinct populations as a potential conservation strategy for the population recovery of this endangered species.

Key words: Cathaya argyrophylla, pollen viability, endangerment, conservation

WANG Hong-Wei, DENG Hui-Sheng, TAN Hai-Ming, QIN Kun, ZHANG Ding-Heng, GE Song. POLLEN VIABILITY AND VARIATION IN CATHAYA ARGYROPHYLLA[J]. Chin J Plant Ecol, 2007, 31(6): 1199-1204.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2007.0149

https://www.plant-ecology.com/EN/Y2007/V31/I6/1199

Figures/Tables 3

References 29

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种群 Population	个体 Individual	雄球花数目 No. of male flowers	花粉活力范围(平均值) Pollen viability (average)
大瑶山1(DYS1)	DYS1-1	4	84.4~92.8 (87.9)
大瑶山3(DYS3)	DYS3-1	3	89.3~92.7 (91.5)
大瑶山4(DYS4)	DYS4-1	5	86.7~93.0 (90.1)
	DYS4-2	1	86.9
			88.5^*
大瑶山5(DYS5)	DYS5-1	3	85.4~86.9(87.7)
	DYS5-2	3	90.1~95.2 (92.4)
	DYS5-5	6	85.9~93.4 (90.9)
	DYS5-6	5	89.1~92.9 (91.2)
	DYS5-8	3	90.2~95.9 (90.3)
			90.5^*
大瑶山7(DYS7)	DYS7-1	1	98.1
大瑶山总数或平均 Total or average in DYS	10	34	91.3
花坪1(HP2)	HP2-1	3	95~96 (95.7)
	HP2-2	3	92~96 (94.0)
	HP2-3	3	92~95 (93.3)
			94.3^*
花坪2(HP3)	HP3-1	3	96 (96)
	HP3-2	3	95~97 (96)
	HP3-3	3	95~97 (96)
			96^*
花坪总数或平均 Total or average in HP	6	18	95.2
物种总数或平均 Total or average in species	16	52	93.3

种群 Population	个体 Individual	雄球花数目 No. of male flowers	花粉活力范围(平均值) Pollen viability (average)
大瑶山1(DYS1)	DYS1-1	4	84.4~92.8 (87.9)
大瑶山3(DYS3)	DYS3-1	3	89.3~92.7 (91.5)
大瑶山4(DYS4)	DYS4-1	5	86.7~93.0 (90.1)
	DYS4-2	1	86.9
			88.5^*
大瑶山5(DYS5)	DYS5-1	3	85.4~86.9(87.7)
	DYS5-2	3	90.1~95.2 (92.4)
	DYS5-5	6	85.9~93.4 (90.9)
	DYS5-6	5	89.1~92.9 (91.2)
	DYS5-8	3	90.2~95.9 (90.3)
			90.5^*
大瑶山7(DYS7)	DYS7-1	1	98.1
大瑶山总数或平均 Total or average in DYS	10	34	91.3
花坪1(HP2)	HP2-1	3	95~96 (95.7)
	HP2-2	3	92~96 (94.0)
	HP2-3	3	92~95 (93.3)
			94.3^*
花坪2(HP3)	HP3-1	3	96 (96)
	HP3-2	3	95~97 (96)
	HP3-3	3	95~97 (96)
			96^*
花坪总数或平均 Total or average in HP	6	18	95.2
物种总数或平均 Total or average in species	16	52	93.3