GENETIC DIVERSITY OF TILIA AMURENSIS POPULATIONS IN DIFFERENT GEOGRAPHICAL DISTRIBUTION REGIONS

doi:10.17521/cjpe.2007.0148

Abstract

Abstract:

Aims Tilia amurensis is one of the II-grade Chinese protected plants with an important economic value and ecological significance. The population of T. amurensis has gradually declined due to overdrafting. Our objectives were to 1) study the genetic diversity of six natural populations varying in longitude and latitude and six varying in altitude and 2) discuss strategies to conserve germplasm resources.

Methods The newly developed inter-simple sequence repeats (ISSR) marker was used to examine genetic diversity.

Important findings The results of 14 ISSR primers showed that polymeric percentage of the six populations was 93.85%, the gene diversity (H) was 0.243 3 and Shannon Index of diversity (I) was 0.380 3. The genetic diversities of T. amurensis decreased with increasing altitude, had a single-peak curve with increasing latitude, and were higher in the central region than in the edges. Analysis of molecular variance (AMOVA) revealed that the majority of genetic variation occurred within populations. Genetic distance and geographic distance were uncorrelated. We concluded that the genetic diversity of T. amurensis was high and mainly within populations. Also, the major factors threatening the development of T. amurensis included direct damage to natural populations due to destruction of habitat and poor regeneration ability caused by innate biological and ecological characteristics. Habitat should be protected, as individuals, especially in core areas.

Key words: Tilia amurensis population, ISSR, genetic diversity, protect strategy

MU Li-Qiang, LIU Ying-Nan. GENETIC DIVERSITY OF TILIA AMURENSIS POPULATIONS IN DIFFERENT GEOGRAPHICAL DISTRIBUTION REGIONS[J]. Chin J Plant Ecol, 2007, 31(6): 1190-1198.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2007.0148

https://www.plant-ecology.com/EN/Y2007/V31/I6/1190

Figures/Tables 10

References 31

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对照 Control	种群 Populations	样本数 Samples	纬度 Latitude (N)	经度 Longitude (E)	海拔 Altitude (m)	生境 Habitats
水平对照	凤凰山(FHS)	15	40°24'37″	124°04'35″	560~580	以辽东栎为主的阔叶混交次生林¹⁾
Level control	长白山(CBS)	15	42°20'56″	128°05'26″	780~820	红松、沙冷杉针阔混交林²⁾
	宁安(NA)	30	44°19'44″	129°38'03″	510~530	以蒙古栎为主的阔叶混交次生林³⁾
	勃利(BL)	30	45°42'02″	130°25'08″	390~420	红松针阔混交林⁴⁾
	凉水(LS)	30	47°10'56″	128°53'28″	300~310	云冷杉红松林⁵⁾
	大杨树(DYS)	15	50°02'22″	124°56'01″	290~320	杂木林⁶⁾
垂直对照	海拔1(H1)	15	42°37'34″	127°56'16″	600~620	红松、沙冷杉针阔混交林²⁾
Upright control	海拔2(H2)	15	42°34'26″	127°50'47″	720~740	红松、沙冷杉针阔混交林²⁾
	海拔3(H3)	15	42°20'56″	128°05'26″	780~820	红松、沙冷杉针阔混交林²⁾
	海拔4(H4)	15	42°18'16″	128°07'42″	900~920	红松、沙冷杉针阔混交林²⁾
	海拔5(H5)	15	42°15'48″	128°09'15″	980~1000	针叶林⁷⁾
	海拔6(H6)	15	42°12'36″	128°10'10″	1 080~1 090	针叶林⁷⁾

对照 Control	种群 Populations	样本数 Samples	纬度 Latitude (N)	经度 Longitude (E)	海拔 Altitude (m)	生境 Habitats
水平对照	凤凰山(FHS)	15	40°24'37″	124°04'35″	560~580	以辽东栎为主的阔叶混交次生林¹⁾
Level control	长白山(CBS)	15	42°20'56″	128°05'26″	780~820	红松、沙冷杉针阔混交林²⁾
	宁安(NA)	30	44°19'44″	129°38'03″	510~530	以蒙古栎为主的阔叶混交次生林³⁾
	勃利(BL)	30	45°42'02″	130°25'08″	390~420	红松针阔混交林⁴⁾
	凉水(LS)	30	47°10'56″	128°53'28″	300~310	云冷杉红松林⁵⁾
	大杨树(DYS)	15	50°02'22″	124°56'01″	290~320	杂木林⁶⁾
垂直对照	海拔1(H1)	15	42°37'34″	127°56'16″	600~620	红松、沙冷杉针阔混交林²⁾
Upright control	海拔2(H2)	15	42°34'26″	127°50'47″	720~740	红松、沙冷杉针阔混交林²⁾
	海拔3(H3)	15	42°20'56″	128°05'26″	780~820	红松、沙冷杉针阔混交林²⁾
	海拔4(H4)	15	42°18'16″	128°07'42″	900~920	红松、沙冷杉针阔混交林²⁾
	海拔5(H5)	15	42°15'48″	128°09'15″	980~1000	针叶林⁷⁾
	海拔6(H6)	15	42°12'36″	128°10'10″	1 080~1 090	针叶林⁷⁾

种群 Population	样本数 No. of sample	多态位点百分率 Percentage of plymerphic P	等位基因数 Observed number of alleles A	有效等位基因数 Effective number of alleles A_e	期望杂合度 Mean expected heterozygosity H	香农信息指数 Shannon's information index I
FHS	15	56.15%	1.561 5	1.278 4	0.169 2	0.261 1
CBS	15	86.92%	1.869 2	1.377 4	0.230 7	0.358 9
NA	30	65.38%	1.653 8	1.319 4	0.194 6	0.299 9
BL	30	70.77%	1.707 7	1.332 3	0.203 5	0.315 8
LS	30	58.46%	1.584 6	1.302 8	0.179 3	0.272 8
DYS	15	57.69%	1.576 9	1.261 7	0.161 2	0.250 8
总 Total	135	93.85%	1.938 5	1.396 1	0.243 3	0.380 3

种群 Population	样本数 No. of sample	多态位点百分率 Percentage of plymerphic P	等位基因数 Observed number of alleles A	有效等位基因数 Effective number of alleles A_e	期望杂合度 Mean expected heterozygosity H	香农信息指数 Shannon's information index I
FHS	15	56.15%	1.561 5	1.278 4	0.169 2	0.261 1
CBS	15	86.92%	1.869 2	1.377 4	0.230 7	0.358 9
NA	30	65.38%	1.653 8	1.319 4	0.194 6	0.299 9
BL	30	70.77%	1.707 7	1.332 3	0.203 5	0.315 8
LS	30	58.46%	1.584 6	1.302 8	0.179 3	0.272 8
DYS	15	57.69%	1.576 9	1.261 7	0.161 2	0.250 8
总 Total	135	93.85%	1.938 5	1.396 1	0.243 3	0.380 3

变异来源 Source of variation	自由度 df	总方差 SSD	均方差 MSD	变异成分 Variance component		显著度检验 p
变异来源 Source of variation	自由度 df	总方差 SSD	均方差 MSD	Absolute	%
种群间 Among populations	5	358.66	71.73	4.36	23.75	<0.002
种群内 Within populations	74	1 035.20	13.98	13.99	76.25	<0.002
总 Total	79			18.35	100