Genetic diversity of arbuscular mycorrhizal fungi in karst microhabitats of Guizhou Province, China

doi:10.3724/SP.J.1258.2011.01083

Abstract

Abstract:

Aims Our objective was to explore the genetic diversity of arbuscular mycorrhizal fungi (AMF) in karst microhabitat.
Methods We used nested polymerase chain reaction and denaturing gradient gel electrophoresis to study the genetic characteristics of AMF sampled from microhabitats of three different vegetation types in Maolan National Nature Reserve of Guizhou Province.
Important findings The AMF genetic diversities in all three microhabitat types were rich. The highest values of biodiversity index (4.06) and species abundance (68) were found in the shrubland soil surface, and the lowest values (3.16 and 29, respectively) were found in the secondary forest rocky crevice. The average values of biodiversity index and species abundance of the 9 microhabitats studied were 3.67 and 48, respectively. These were much higher than those in some other areas; the possible reasons were the complex karst ecological system structure and high plant species diversity. The AMF genetic diversity of different microhabitat types showed significant differences. The highest similarity index was only 0.45, and this means the spatial heterogeneity of different microhabitat types had significant influence on AMF genetic diversity. Glomus was the dominant genus through the karst area and should be a candidate for screening high ecological restoration strains for karst areas.

Key words: arbuscular mycorrhizal fungi, genetic diversity, karst, microhabitat, richness

WEI Yuan, WANG Shi-Jie, LIU Xiu-Ming, HUANG Tian-Zhi. Genetic diversity of arbuscular mycorrhizal fungi in karst microhabitats of Guizhou Province, China[J]. Chin J Plant Ecol, 2011, 35(10): 1083-1090.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.01083

https://www.plant-ecology.com/EN/Y2011/V35/I10/1083

Figures/Tables 5

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引物 Primer	序列 Sequence	长度 Length	来源 Sources
GeoA2	5′-CCAGTAGTCATATGCTTGTCTC-3′	1.80 kb	Schwarzott & Schüβler, 2001
Geo11	5′-ACCTTGTTACGACTTTTACTTCC-3′	1.80 kb	Schwarzott & Schüβler, 2001
AM1	5′-GTTTCCCGTAAGGCGCCGAA-3′	0.55 kb	Helgason et al., 1998 Simon et al., 1992
NS31-GC	5′-TTGGAGGGCAAGTCTGGTGCC-3′	0.55 kb	Helgason et al., 1998 Simon et al., 1992
Glol	5′-GCCTGCTTTAAACACTCTA-3′	0.23 kb	Comejo et al., 2004

引物 Primer	序列 Sequence	长度 Length	来源 Sources
GeoA2	5′-CCAGTAGTCATATGCTTGTCTC-3′	1.80 kb	Schwarzott & Schüβler, 2001
Geo11	5′-ACCTTGTTACGACTTTTACTTCC-3′	1.80 kb	Schwarzott & Schüβler, 2001
AM1	5′-GTTTCCCGTAAGGCGCCGAA-3′	0.55 kb	Helgason et al., 1998 Simon et al., 1992
NS31-GC	5′-TTGGAGGGCAAGTCTGGTGCC-3′	0.55 kb	Helgason et al., 1998 Simon et al., 1992
Glol	5′-GCCTGCTTTAAACACTCTA-3′	0.23 kb	Comejo et al., 2004

样品 Sample	Shannon多样性指数 Shannon’s diversity index	丰富度 Richness	均匀度 Evenness
原生林石缝 Original forest rocky crevice	3.89	57	0.96
原生林石沟 Original forest rocky gully	3.74	48	0.97
原生林土面 Original forest soil surface	3.33	36	0.93
次生林石缝 Secondary forest rocky crevice	3.16	29	0.94
次生林石沟 Secondary forest rocky gully	3.65	48	0.94
次生林土面 Secondary forest soil surface	3.71	50	0.95
灌木林石缝 Shrubbery rocky crevice	3.68	50	0.94
灌木林石沟 Shrubbery rocky gully	3.77	48	0.97
灌木林土面 Shrubbery soil surface	4.06	68	0.96

样品 Sample	Shannon多样性指数 Shannon’s diversity index	丰富度 Richness	均匀度 Evenness
原生林石缝 Original forest rocky crevice	3.89	57	0.96
原生林石沟 Original forest rocky gully	3.74	48	0.97
原生林土面 Original forest soil surface	3.33	36	0.93
次生林石缝 Secondary forest rocky crevice	3.16	29	0.94
次生林石沟 Secondary forest rocky gully	3.65	48	0.94
次生林土面 Secondary forest soil surface	3.71	50	0.95
灌木林石缝 Shrubbery rocky crevice	3.68	50	0.94
灌木林石沟 Shrubbery rocky gully	3.77	48	0.97
灌木林土面 Shrubbery soil surface	4.06	68	0.96

条带来源(GenBank登录号) Band source (GenBank accession number)	最近相似种属 Closest genera	相似性 Similarity
原生林石缝 Original forest rocky crevice (JN153048)	用DGGE方法分离的属于未培养的球囊霉属的18S RNA菌种 Uncultured Glomus isolate DGGE gel band 22-1 18S ribosomal RNA gene (HQ874638)	88%
原生林石沟 Original forest rocky gully (JN153041)	用DGGE方法分离的属于未培养的球囊霉属的18S RNA菌种 Uncultured Glomus isolate DGGE gel band 11-2 18S ribosomal RNA gene (HQ874635)	86%
原生林土面 Original forest soil surface (JN153042)	用克隆方法分离的属于未培养的球囊霉属的18S RNA菌种 Uncultured Glomus clone OEF89 18S ribosomal RNA gene (EU340303)	99%
次生林石缝 Secondary forest rocky crevice (JN153043)	用DGGE方法分离的属于未培养的球囊霉属的18S RNA菌种 Uncultured Glomus isolate DGGE gel band 22-2 18S ribosomal RNA gene (HQ874639)	95%
次生林石沟 Secondary forest rocky gully (JN153044)	用克隆方法分离的属于未培养的球囊霉属的18S RNA菌种 Uncultured Glomus clone Ap7 18S ribosomal RNA gene (EU350053)	95%
次生林土面 Secondary forest soil surface (JN153040)	用克隆方法分离的属于未培养的球囊霉属的18S RNA菌种 Uncultured Glomus clone FEA6HBX02GK5NA 18S ribosomal RNA gene (GU198582)	93%
灌木林石缝 Shrubbery rocky crevice (JN153045)	未培养的球囊霉属的18S RNA菌种 Uncultured Glomus gene for 18S ribosomal RNA (AB556933)	97%
灌木林石沟 Shrubbery rocky gully (JN153046)	未培养的球囊霉属的18S RNA菌种, Uncultured Glomus partial 18S rRNA gene (AJ563889)	96%
灌木林土面 Shrubbery soil surface (JN153047)	用DGGE方法分离的属于未培养的球囊霉属的18S RNA菌种 Uncultured Glomus isolate DGGE gel band 22-2 18S ribosomal RNA gene (HQ874639)	91%