Chin J Plan Ecolo ›› 2017, Vol. 41 ›› Issue (7): 787-794.DOI: 10.17521/cjpe.2016.0322

• Method and Technology • Previous Articles     Next Articles

A new angiosperms molecular specimen treatment method for field use

Feng-Jiao SHEN, Qian-Qian REN, Qi DONG, Li ZHU, Jian-Fang ZHANG, Jing YANG, Ran ZHANG, Hong-Zhu LIANG, Jian-Cheng ZHAO, Shuo SHI*()   

  1. College of Life Science, Hebei Normal University, Shijiazhuang 050024, China
  • Received:2016-10-17 Accepted:2017-06-01 Online:2017-07-10 Published:2017-08-21
  • Contact: Shuo SHI
  • About author:

    KANG Jing-yao(1991-), E-mail:


Aims This study aims to identify a more convenient drying method for obtaining molecular specimen of angiosperms in the field than the conventional silica gel drying method. Methods The leaves of Prunus serrulata var. lannesiana and Liriope spicata were dried under temperatures of 150 °C, 80 °C, 40 °C as well as under natural conditions, and by the silica gel drying method, respectively. The DNA extracts of various specimens were then analyzed using techniques of spectrophotometer detection, electrophoresis and PCR to evaluate the impacts of different drying treatments to the genomic DNA of testing plants. Important findings The concentrations of total DNA were higher for the specimens dried at 40 °C treatment and by the silica gel drying method than other treatments when assessed by the techniques of spectrophotometer detection and electrophoresis. The concentration of PCR products was highest in the specimens dried at 40 °C. Based on the results, the 40 °C drying can be recommended for obtaining molecular specimens of angiosperms because of its minimum degree of degradation, for convenience of operation and avoiding carrying large amounts of silica gel in field investigations.

Key words: angiosperms, field, molecular specimen, DNA, drying, silica gel