Physiological responses of bryophytes experienced low temperature stress to simulated nitrogen deposition

doi:10.3724/SP.J.1258.2011.00268

Abstract

Abstract:

Aims Increasing N deposition and frequent abnormal weather are two characteristics of global climate change. Our objective was to study the physiological responses of bryophytes to simulated nitrogen treatments and low temperature stress to provide insights to the relationship between N deposition and compensatory effect after low temperature stress.
Methods Physiological responses of three bryophyte species, Pogonatum cirratum subsp. fuscatum, Hypnum plumaeforme and Reboulia hemisphaerica, which were subjected to a 2-year-simulated N deposition and suffered a low temperature stress in early 2008, were studied and compared with the results of the bryophytes that experienced a 1-year-simulated N deposition and normal weather conditions. N treatments (Control, 20, 40 and 60 kg N·hm^-2·a^-1) in three replicates were established for each species. The N additions were divided into four applications per year.
Important findings The results of 2008 showed that net photosynthetic rate and concentration of starch decreased with increasing N addition doses within the range of 0-60 kg N·hm^-2·a^-1. The concentration of total N increased with increasing N treatment doses within the range of 0-40 kg N·hm^-2·a^-1, but decreased at N addition doses of 60 kg N·hm^-2·a^-1. At control and low N addition conditions (20 kg N·hm^-2·a^-1), most of the indices of carbon and nitrogen metabolism of the three bryophytes were higher than the results of 2007 of the same species at the same N treatments, but N addition depressed the increase. At high N treatment conditions (≥ 40 kg N·hm^-2·a^-1), the results of 2007 and 2008 were usually similar. Results indicate that the bryophytes exhibit overcompensatory growth after experiencing a low temperature stress, but at stimulated N deposition conditions, their compensatory ability decreases.

Key words: bryophytes, compensatory effect, low temperature stress, nitrogen deposition

LIU Bin-Yang, LIU Wei-Qiu, ZHANG Yi-Shun, LEI Chun-Yi. Physiological responses of bryophytes experienced low temperature stress to simulated nitrogen deposition[J]. Chin J Plant Ecol, 2011, 35(3): 268-274.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00268

https://www.plant-ecology.com/EN/Y2011/V35/I3/268

Figures/Tables 4

Fig. 1 Daily mean air temperature (A), daily maximum air temperature (B), daily minimum air temperature (C) at Heishiding Nature Reserve for 40 days before the measurement of physiological indices in 2007 and 2008. Data collected from the meteorological monitoring station at Heishiding Nature Reserve. The real lines represent data from Nov. 25, 2006 to Jan. 3, 2007, the dashed lines represent data from Jan. 31, 2008 to Mar. 8, 2008.

Fig. 2 Indices of carbon metabolism in bryophytes after two years of simulated nitrogen deposition (mean ± SD, n = 3). A and D, Pogonatum cirratum subsp. fuscatum. B and E, Hypnum plumaeforme. C and F, Reboulia hemisphaerica. Values with different letters are significantly different (p < 0.05, One-way ANOVA) in each species and each dependent variable.

Fig. 3 Indices of N metabolism in three bryophyte species in 2008 after two years of simulated nitrogen deposition (mean ± SD, n = 3). A, Pogonatum cirratum subsp. fuscatum. B, Hypnum plumaeforme. C, Reboulia hemisphaerica.

Table 1 Nitrate reductase activity (NRA) in Pogonatum cirratum subsp. fuscatum and Hypnum plumaeforme in 2008 after two years of simulated nitrogen deposition (mean ± SD, n = 3)

加N量 N addition doses (kg N·hm^-2·a^-1)	NRA (μg N·g^-1FW·h^-1)
加N量 N addition doses (kg N·hm^-2·a^-1)	固有活性 Constitutive NRA	诱导活性 Inducible NRA
刺边小金发藓拟刺亚种 P. cirratum subsp. fuscatum
0	3.86 ± 0.06^a	2.82 ± 0.48^a
20	1.80 ± 0.15^b	-0.34 ± 0.51^b
40	1.04 ± 0.20^c	-0.59 ± 0.10^b
60	0.54 ± 0.28^d	-0.54 ± 0.33^b
大灰藓 H. plumaeforme
0	3.86 ± 0.28^a	14.14 ± 0.70^a
20	1.58 ± 0.17^b	1.18 ± 0.13^b
40	1.48 ± 0.42^b	-0.22 ± 0.02^c
60	1.14 ± 0.08^b	-0.06 ± 0.18^c

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