Functional diversity of soil microorganisms in Casuarina equisetifolia woodlands of different stand ages in Hainan Island

doi:10.3724/SP.J.1258.2014.00056

Abstract

Abstract:

Aims Soil microbes play a key role in matter recycling and accumulation of allelochemicals. We studied the functional diversity of microbial communities in order to illustrate variations in soil microbial community diversity among different Casuarina equisetifolia woodlands.
Methods Young, middle-aged, and mature forest of C. equisetifolia woodlands in the Haikou Guilinyang littoral zone were chosen as subjects of the study. Soil microbial functional diversity in those stands was determined by using Biolog micro-plate technique.
Important findings The soil microbial carbon utilization was higher in bare land and the mature forest than the middle-aged and young forest. With increasing stand age, the values of Shannon-Wiener index (H') and McIntosh and Simpson diversity indices all increased. The H' significantly differed among different woodlands. However, there were no significant differences in the McIntosh and Simpson diversity indices between the young and middle-aged forest. Principal component analysis demonstrated that there was a significant difference in the soil microbial metabolic activities among different woodlands, in particular concerning monosaccharides and amino acids. The soil microbial community diversity increased with increasing stand age, which might be due to a joint action among many factors such as litter, the secondary metabolites of plant root secretion, soil nutrients, and specific soil microorganisms.

Key words: Biolog-ECO, Casuarina equisetifolia, functional diversity, soil microorganisms

LI Xiao-Rong,WEI Jin-Yu,CHEN Yun,CAO Ting-Ting,FENG Li,GU Mei-Zi,LI Lei. Functional diversity of soil microorganisms in Casuarina equisetifolia woodlands of different stand ages in Hainan Island[J]. Chin J Plant Ecol, 2014, 38(6): 608-618.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2014.00056

https://www.plant-ecology.com/EN/Y2014/V38/I6/608

Figures/Tables 8

References 47

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林地 Woodland	容重 Bulk density (g·cm^-3)	含水量 Water content (%)	pH值 pH value	全氮 Total N (mg·kg^-1)	全磷 Total P (mg·kg^-1)	全钾 Total K (mg·kg^-1)
对照裸地 Bare land as control	1.52 ± 0.05^a	13.35 ± 0.69^b	5.52 ± 0.26^c	297.49 ± 47.11^b	528.31 ± 2.65^c	137.82 ± 24.62^b
幼龄林 Young forest	1.27 ± 0.06^b	14.88 ± 1.15^a	5.27 ± 0.45^bc	435.65 ± 11.76^a	498.62 ± 5.30^d	175.55 ± 30.42^a
中龄林 Middle-aged forest	1.30 ± 0.03^b	13.39 ± 0.45^b	4.96 ± 0.29^ab	418.32 ± 31.45^a	552.76 ± 7.05^b	154.67 ± 6.21^ab
成熟林 Mature forest	1.28 ± 0.02^b	14.62 ± 0.65^a	4.68 ± 0.15^a	239.91 ± 24.00^b	573.10 ± 0.47^a	155.40 ± 22.83^ab

林地 Woodland	容重 Bulk density (g·cm^-3)	含水量 Water content (%)	pH值 pH value	全氮 Total N (mg·kg^-1)	全磷 Total P (mg·kg^-1)	全钾 Total K (mg·kg^-1)
对照裸地 Bare land as control	1.52 ± 0.05^a	13.35 ± 0.69^b	5.52 ± 0.26^c	297.49 ± 47.11^b	528.31 ± 2.65^c	137.82 ± 24.62^b
幼龄林 Young forest	1.27 ± 0.06^b	14.88 ± 1.15^a	5.27 ± 0.45^bc	435.65 ± 11.76^a	498.62 ± 5.30^d	175.55 ± 30.42^a
中龄林 Middle-aged forest	1.30 ± 0.03^b	13.39 ± 0.45^b	4.96 ± 0.29^ab	418.32 ± 31.45^a	552.76 ± 7.05^b	154.67 ± 6.21^ab
成熟林 Mature forest	1.28 ± 0.02^b	14.62 ± 0.65^a	4.68 ± 0.15^a	239.91 ± 24.00^b	573.10 ± 0.47^a	155.40 ± 22.83^ab

林地 Woodland	单糖类底物及其衍生物 Monosaccharide substrates and their derivants	二糖、多糖底物Disaccharide and polysaccharide substrates	氨基酸底物及其衍生物 Amino acid substrates and their derivants	脂肪酸和脂类 Fat acid and lipid	代谢中间产物和次生代谢物 Metabolic intermediates and secondary metabolites
对照裸地 Bare land as control	2.27 ± 0.12^a	1.14 ± 0.29^a	2.85 ± 0.10^a	2.22 ± 0.17^a	2.19 ± 0.07^a
幼龄林 Young forest	0.28 ± 0.17^c	0.22 ± 0.20^b	0.02 ± 0.03^d	0.49 ± 0.18^b	0.23 ± 0.04^c
中龄林 Middle-aged forest	0.30 ± 0.07^c	0.03 ± 0.00^b	0.43 ± 0.19^c	0.78 ± 0.39^b	0.40 ± 0.13^c
成熟林 Mature forest	1.89 ± 0.17^b	0.82 ± 0.21^a	2.46 ± 0.20^b	2.04 ± 0.07^a	1.69 ± 0.76^b

林地 Woodland	单糖类底物及其衍生物 Monosaccharide substrates and their derivants	二糖、多糖底物Disaccharide and polysaccharide substrates	氨基酸底物及其衍生物 Amino acid substrates and their derivants	脂肪酸和脂类 Fat acid and lipid	代谢中间产物和次生代谢物 Metabolic intermediates and secondary metabolites
对照裸地 Bare land as control	2.27 ± 0.12^a	1.14 ± 0.29^a	2.85 ± 0.10^a	2.22 ± 0.17^a	2.19 ± 0.07^a
幼龄林 Young forest	0.28 ± 0.17^c	0.22 ± 0.20^b	0.02 ± 0.03^d	0.49 ± 0.18^b	0.23 ± 0.04^c
中龄林 Middle-aged forest	0.30 ± 0.07^c	0.03 ± 0.00^b	0.43 ± 0.19^c	0.78 ± 0.39^b	0.40 ± 0.13^c
成熟林 Mature forest	1.89 ± 0.17^b	0.82 ± 0.21^a	2.46 ± 0.20^b	2.04 ± 0.07^a	1.69 ± 0.76^b

林地 Woodland	Shannon-Wiener指数 Shannon-Wiener index	Simpson指数 Simpson index	McIntosh指数 McIntosh index	丰富度指数 Richness index
对照裸地 Bare land as control	3.17 ± 0.02^d	21.92 ± 0.12^b	12.87 ± 0.25^b	29.00 ± 1.00^a
幼龄林 Young forest	2.28 ± 0.06^c	8.23 ± 0.36^c	4.69 ± 0.31^c	16.67 ± 0.58^b
中龄林 Middle-aged forest	2.53 ± 0.06^b	9.13 ± 1.61^c	5.12 ± 0.07^c	8.00 ± 2.00^c
成熟林 Mature forest	3.29 ± 0.04^a	25.54 ± 1.35^a	13.81 ± 0.01^a	27.67 ± 1.53^a