Chin J Plant Ecol ›› 2008, Vol. 32 ›› Issue (4): 938-950.DOI: 10.3773/j.issn.1005-264x.2008.04.024
Special Issue: 生物多样性
• Original article • Previous Articles Next Articles
YAN Mao-Fen, LI Xiang-Hua, WANG Ke-Jing()
Received:
2006-10-18
Accepted:
2007-11-06
Online:
2008-10-18
Published:
2008-07-30
Contact:
WANG Ke-Jing
YAN Mao-Fen, LI Xiang-Hua, WANG Ke-Jing. EVALUATION OF GENETIC DIVERSITY BY SSR MARKERS FOR NATURAL POPULATIONS OF WILD SOYBEAN (GLYCINE SOJA) GROWING IN THE REGION OF BEIJING, CHINA[J]. Chin J Plant Ecol, 2008, 32(4): 938-950.
种群Population | 收集地点 Collection site | 取样株数 Size of sample | 纬度Latitude (N) | 海拔Altitude (m) | 生境 Habitat | 分布面积 Area (m2) |
---|---|---|---|---|---|---|
1 | 延庆白河堡村 Baihepu, Yanqing | 30 | 40°37′847′′ | 586 | 白河水库边 Baihe Reservoir | 3 000 |
2 | 怀柔牦牛沟 Maoniugou, Huairou | 28 | 40°42′534′′ | 322 | 小河岸边 Riverside | 400 |
3 | 密云石城镇 Shicheng, Miyun | 30 | 40°33′741′′ | 135 | 白河岸边 Riverside | 3 000 |
4 | 平谷刘家河村 Liujiahe, Pingu | 30 | 40°11′871′′ | 89 | 干河堤 Drought riverbank | 1 000 |
5 | 顺义北营村 Beiying, Shunyi | 30 | 40°09′048′′ | 68 | 路边 Roadside | 1 000 |
6 | 石景山麻峪村 Mayu, Shijingshan | 30 | 39°56′253′′ | 91 | 永定河 River | 2 000 |
7 | 门头沟苇甸村 Weidian, Mentougou | 30 | 39°58′641′′ | 150 | 山区路边 Roadside | 500 |
8 | 昌平北小营村 Beixiaoying, Changping | 30 | 40°10′846′′ | 59 | 路旁沟中 Roadside trench | 1 000 |
9 | 朝阳三岔河村 Sanchahe, Chaoyang | 30 | 39°58′188′′ | 19 | 坝河岸上 Riverbank | 1 000 |
10 | 丰台卢沟桥 Lugouqiao, Fengtai | 30 | 39°50′819′′ | 26 | 永定河河床 River bed | 2 000 |
Table 1 Collection sites and habitats of 10 natural populations of wild soybean in Beijing
种群Population | 收集地点 Collection site | 取样株数 Size of sample | 纬度Latitude (N) | 海拔Altitude (m) | 生境 Habitat | 分布面积 Area (m2) |
---|---|---|---|---|---|---|
1 | 延庆白河堡村 Baihepu, Yanqing | 30 | 40°37′847′′ | 586 | 白河水库边 Baihe Reservoir | 3 000 |
2 | 怀柔牦牛沟 Maoniugou, Huairou | 28 | 40°42′534′′ | 322 | 小河岸边 Riverside | 400 |
3 | 密云石城镇 Shicheng, Miyun | 30 | 40°33′741′′ | 135 | 白河岸边 Riverside | 3 000 |
4 | 平谷刘家河村 Liujiahe, Pingu | 30 | 40°11′871′′ | 89 | 干河堤 Drought riverbank | 1 000 |
5 | 顺义北营村 Beiying, Shunyi | 30 | 40°09′048′′ | 68 | 路边 Roadside | 1 000 |
6 | 石景山麻峪村 Mayu, Shijingshan | 30 | 39°56′253′′ | 91 | 永定河 River | 2 000 |
7 | 门头沟苇甸村 Weidian, Mentougou | 30 | 39°58′641′′ | 150 | 山区路边 Roadside | 500 |
8 | 昌平北小营村 Beixiaoying, Changping | 30 | 40°10′846′′ | 59 | 路旁沟中 Roadside trench | 1 000 |
9 | 朝阳三岔河村 Sanchahe, Chaoyang | 30 | 39°58′188′′ | 19 | 坝河岸上 Riverbank | 1 000 |
10 | 丰台卢沟桥 Lugouqiao, Fengtai | 30 | 39°50′819′′ | 26 | 永定河河床 River bed | 2 000 |
引物 Primer | 带数 No. of bands | 种群 Population | 引物 Primer | 带数 No. of bands | 种群 Population | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |||||
satt236 | 9 | 5 | 1 | 7 | 1 | 7 | 1 | 4 | 5 | 5 | 2 | satt309 | 12 | 2 | 1 | 4 | 2 | 2 | 1 | 3 | 3 | 3 | 1 | |
satt300 | 16 | 3 | 1 | 4 | 2 | 3 | 2 | 6 | 7 | 2 | 2 | satt279 | 14 | 2 | 1 | 2 | 1 | 5 | 3 | 5 | 2 | 3 | 3 | |
satt390 | 13 | 1 | 1 | 4 | 1 | 5 | 2 | 4 | 7 | 3 | 3 | satt434 | 11 | 3 | 1 | 3 | 4 | 4 | 1 | 8 | 8 | 2 | 2 | |
satt429 | 12 | 4 | 1 | 8 | 1 | 7 | 1 | 3 | 3 | 3 | 3 | satt239 | 10 | 2 | 1 | 4 | 2 | 3 | 1 | 2 | 3 | 2 | 2 | |
satt453 | 11 | 2 | 1 | 7 | 1 | 5 | 2 | 3 | 4 | 2 | 2 | satt571 | 18 | 2 | 1 | 6 | 1 | 8 | 1 | 4 | 3 | 3 | 2 | |
satt197 | 3 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | satt431 | 17 | 3 | 2 | 3 | 1 | 6 | 5 | 4 | 4 | 5 | 3 | |
att168 | 12 | 1 | 2 | 2 | 1 | 5 | 2 | 5 | 5 | 4 | 5 | satt414 | 17 | 1 | 1 | 4 | 2 | 6 | 2 | 5 | 4 | 1 | 2 | |
satt556 | 16 | 4 | 1 | 6 | 1 | 8 | 4 | 6 | 4 | 4 | 4 | satt242 | 13 | 3 | 1 | 2 | 1 | 5 | 2 | 3 | 4 | 4 | 2 | |
satt194 | 14 | 4 | 1 | 5 | 1 | 3 | 3 | 4 | 3 | 2 | 2 | sct-099 | 15 | 2 | 1 | 4 | 1 | 4 | 3 | 5 | 7 | 4 | 4 | |
Satt180 | 9 | 2 | 1 | 3 | 1 | 3 | 1 | 2 | 2 | 1 | 2 | satt373 | 12 | 2 | 1 | 4 | 1 | 4 | 3 | 2 | 4 | 3 | 1 | |
satt307 | 14 | 6 | 2 | 6 | 1 | 2 | 1 | 3 | 3 | 3 | 3 | satt346 | 16 | 2 | 1 | 4 | 1 | 1 | 2 | 4 | 3 | 3 | 2 | |
satt281 | 19 | 4 | 1 | 6 | 1 | 6 | 3 | 3 | 6 | 4 | 5 | satt590 | 13 | 2 | 1 | 4 | 1 | 2 | 1 | 4 | 7 | 3 | 3 | |
satt386 | 15 | 3 | 1 | 2 | 1 | 3 | 3 | 3 | 6 | 3 | 2 | satt339 | 8 | 3 | 1 | 3 | 1 | 3 | 2 | 4 | 3 | 1 | 3 | |
satt002 | 17 | 2 | 1 | 7 | 1 | 6 | 2 | 7 | 5 | 3 | 1 | satt530 | 19 | 5 | 1 | 6 | 1 | 5 | 1 | 5 | 5 | 4 | 1 | |
satt268 | 11 | 3 | 2 | 4 | 1 | 3 | 1 | 5 | 5 | 3 | 4 | satt243 | 12 | 4 | 1 | 5 | 1 | 7 | 4 | 3 | 4 | 3 | 5 | |
satt230 | 9 | 9 | 1 | 6 | 1 | 4 | 4 | 4 | 3 | 4 | 4 | satt487 | 16 | 3 | 1 | 6 | 1 | 6 | 3 | 6 | 5 | 3 | 3 | |
satt185 | 13 | 4 | 1 | 4 | 1 | 4 | 1 | 3 | 3 | 2 | 2 | satt345 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | |
satt146 | 14 | 2 | 1 | 6 | 2 | 3 | 1 | 4 | 1 | 3 | 2 | satt216 | 15 | 5 | 1 | 2 | 1 | 1 | 2 | 5 | 4 | 4 | 3 | |
satt334 | 10 | 2 | 1 | 2 | 1 | 3 | 1 | 3 | 1 | 1 | 1 | satt005 | 14 | 2 | 2 | 8 | 1 | 3 | 3 | 5 | 3 | 3 | 2 | |
satt352 | 11 | 3 | 1 | 4 | 1 | 3 | 1 | 4 | 2 | 2 | 2 | satt267 | 8 | 1 | 1 | 4 | 1 | 5 | 2 | 2 | 3 | 2 | 3 | |
各居群在40对引物上的总带数 Total | 526 | 116 | 45 | 175 | 48 | 165 | 80 | 157 | 157 | 113 | 100 |
Table 2 The number of bands produced by every primer in the populations
引物 Primer | 带数 No. of bands | 种群 Population | 引物 Primer | 带数 No. of bands | 种群 Population | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |||||
satt236 | 9 | 5 | 1 | 7 | 1 | 7 | 1 | 4 | 5 | 5 | 2 | satt309 | 12 | 2 | 1 | 4 | 2 | 2 | 1 | 3 | 3 | 3 | 1 | |
satt300 | 16 | 3 | 1 | 4 | 2 | 3 | 2 | 6 | 7 | 2 | 2 | satt279 | 14 | 2 | 1 | 2 | 1 | 5 | 3 | 5 | 2 | 3 | 3 | |
satt390 | 13 | 1 | 1 | 4 | 1 | 5 | 2 | 4 | 7 | 3 | 3 | satt434 | 11 | 3 | 1 | 3 | 4 | 4 | 1 | 8 | 8 | 2 | 2 | |
satt429 | 12 | 4 | 1 | 8 | 1 | 7 | 1 | 3 | 3 | 3 | 3 | satt239 | 10 | 2 | 1 | 4 | 2 | 3 | 1 | 2 | 3 | 2 | 2 | |
satt453 | 11 | 2 | 1 | 7 | 1 | 5 | 2 | 3 | 4 | 2 | 2 | satt571 | 18 | 2 | 1 | 6 | 1 | 8 | 1 | 4 | 3 | 3 | 2 | |
satt197 | 3 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | satt431 | 17 | 3 | 2 | 3 | 1 | 6 | 5 | 4 | 4 | 5 | 3 | |
att168 | 12 | 1 | 2 | 2 | 1 | 5 | 2 | 5 | 5 | 4 | 5 | satt414 | 17 | 1 | 1 | 4 | 2 | 6 | 2 | 5 | 4 | 1 | 2 | |
satt556 | 16 | 4 | 1 | 6 | 1 | 8 | 4 | 6 | 4 | 4 | 4 | satt242 | 13 | 3 | 1 | 2 | 1 | 5 | 2 | 3 | 4 | 4 | 2 | |
satt194 | 14 | 4 | 1 | 5 | 1 | 3 | 3 | 4 | 3 | 2 | 2 | sct-099 | 15 | 2 | 1 | 4 | 1 | 4 | 3 | 5 | 7 | 4 | 4 | |
Satt180 | 9 | 2 | 1 | 3 | 1 | 3 | 1 | 2 | 2 | 1 | 2 | satt373 | 12 | 2 | 1 | 4 | 1 | 4 | 3 | 2 | 4 | 3 | 1 | |
satt307 | 14 | 6 | 2 | 6 | 1 | 2 | 1 | 3 | 3 | 3 | 3 | satt346 | 16 | 2 | 1 | 4 | 1 | 1 | 2 | 4 | 3 | 3 | 2 | |
satt281 | 19 | 4 | 1 | 6 | 1 | 6 | 3 | 3 | 6 | 4 | 5 | satt590 | 13 | 2 | 1 | 4 | 1 | 2 | 1 | 4 | 7 | 3 | 3 | |
satt386 | 15 | 3 | 1 | 2 | 1 | 3 | 3 | 3 | 6 | 3 | 2 | satt339 | 8 | 3 | 1 | 3 | 1 | 3 | 2 | 4 | 3 | 1 | 3 | |
satt002 | 17 | 2 | 1 | 7 | 1 | 6 | 2 | 7 | 5 | 3 | 1 | satt530 | 19 | 5 | 1 | 6 | 1 | 5 | 1 | 5 | 5 | 4 | 1 | |
satt268 | 11 | 3 | 2 | 4 | 1 | 3 | 1 | 5 | 5 | 3 | 4 | satt243 | 12 | 4 | 1 | 5 | 1 | 7 | 4 | 3 | 4 | 3 | 5 | |
satt230 | 9 | 9 | 1 | 6 | 1 | 4 | 4 | 4 | 3 | 4 | 4 | satt487 | 16 | 3 | 1 | 6 | 1 | 6 | 3 | 6 | 5 | 3 | 3 | |
satt185 | 13 | 4 | 1 | 4 | 1 | 4 | 1 | 3 | 3 | 2 | 2 | satt345 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | |
satt146 | 14 | 2 | 1 | 6 | 2 | 3 | 1 | 4 | 1 | 3 | 2 | satt216 | 15 | 5 | 1 | 2 | 1 | 1 | 2 | 5 | 4 | 4 | 3 | |
satt334 | 10 | 2 | 1 | 2 | 1 | 3 | 1 | 3 | 1 | 1 | 1 | satt005 | 14 | 2 | 2 | 8 | 1 | 3 | 3 | 5 | 3 | 3 | 2 | |
satt352 | 11 | 3 | 1 | 4 | 1 | 3 | 1 | 4 | 2 | 2 | 2 | satt267 | 8 | 1 | 1 | 4 | 1 | 5 | 2 | 2 | 3 | 2 | 3 | |
各居群在40对引物上的总带数 Total | 526 | 116 | 45 | 175 | 48 | 165 | 80 | 157 | 157 | 113 | 100 |
种群 Population | 多态位点百分数 P (%) | 平均预期杂合度He | 实际观察杂合度 Ho | 平均等位基因 A | 有效等位基因 Ae | Shanno指数 I |
---|---|---|---|---|---|---|
1 | 87.50 | 0.446 3 | 0.006 7 | 2.900 0 | 2.145 8 | 0.743 9 |
2 | 12.50 | 0.017 3 | 0.000 9 | 1.125 0 | 1.023 4 | 0.030 4 |
3 | 100.00 | 0.603 1 | 0.018 3 | 4.375 0 | 3.120 8 | 1.137 9 |
4 | 15.00 | 0.033 6 | 0.000 0 | 1.200 0 | 1.074 5 | 0.058 8 |
5 | 90.00 | 0.580 4 | 0.029 2 | 4.125 0 | 3.011 6 | 1.083 8 |
6 | 57.50 | 0.221 7 | 0.004 2 | 2.000 0 | 1.454 8 | 0.365 6 |
7 | 95.00 | 0.458 4 | 0.019 2 | 3.925 0 | 2.050 7 | 0.853 4 |
8 | 92.50 | 0.575 7 | 0.010 8 | 3.925 0 | 2.799 5 | 1.049 0 |
9 | 87.50 | 0.400 7 | 0.025 0 | 2.825 0 | 1.843 6 | 0.677 3 |
10 | 82.50 | 0.347 9 | 0.015 0 | 2.500 0 | 1.766 7 | 0.582 4 |
平均 Mean | 72.00 | 0.368 5 | 0.012 9 | 2.890 0 | 2.029 1 | 0.658 3 |
Table 3 Estimates of genetic parameters for 10 natural populations of wild soybean in Beijing
种群 Population | 多态位点百分数 P (%) | 平均预期杂合度He | 实际观察杂合度 Ho | 平均等位基因 A | 有效等位基因 Ae | Shanno指数 I |
---|---|---|---|---|---|---|
1 | 87.50 | 0.446 3 | 0.006 7 | 2.900 0 | 2.145 8 | 0.743 9 |
2 | 12.50 | 0.017 3 | 0.000 9 | 1.125 0 | 1.023 4 | 0.030 4 |
3 | 100.00 | 0.603 1 | 0.018 3 | 4.375 0 | 3.120 8 | 1.137 9 |
4 | 15.00 | 0.033 6 | 0.000 0 | 1.200 0 | 1.074 5 | 0.058 8 |
5 | 90.00 | 0.580 4 | 0.029 2 | 4.125 0 | 3.011 6 | 1.083 8 |
6 | 57.50 | 0.221 7 | 0.004 2 | 2.000 0 | 1.454 8 | 0.365 6 |
7 | 95.00 | 0.458 4 | 0.019 2 | 3.925 0 | 2.050 7 | 0.853 4 |
8 | 92.50 | 0.575 7 | 0.010 8 | 3.925 0 | 2.799 5 | 1.049 0 |
9 | 87.50 | 0.400 7 | 0.025 0 | 2.825 0 | 1.843 6 | 0.677 3 |
10 | 82.50 | 0.347 9 | 0.015 0 | 2.500 0 | 1.766 7 | 0.582 4 |
平均 Mean | 72.00 | 0.368 5 | 0.012 9 | 2.890 0 | 2.029 1 | 0.658 3 |
位点Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传 多样度Hs | 居群间遗传多样度DST | 基因分 化度GST | 位点 Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传多样度Hs | 居群间遗传 多样度DST | 基因分 化度GST |
---|---|---|---|---|---|---|---|---|---|---|---|
satt236 | A1 | 0.850 1 | 0.422 4 | 0.345 3 | 0.449 8 | satt309 | G | 0.466 7 | 0.282 3 | 0.569 7 | 0.668 7 |
satt300 | A1 | 0.746 2 | 0.414 5 | 0.434 8 | 0.512 0 | satt279 | H | 0.756 0 | 0.437 8 | 0.407 5 | 0.482 1 |
satt390 | A2 | 0.749 5 | 0.402 8 | 0.432 6 | 0.517 8 | satt434 | H | 0.759 6 | 0.403 3 | 0.432 5 | 0.517 5 |
satt429 | A2 | 0.767 3 | 0.390 7 | 0.443 5 | 0.531 6 | satt239 | I | 0.503 0 | 0.300 2 | 0.424 6 | 0.585 8 |
satt453 | B1 | 0.710 5 | 0.403 5 | 0.464 6 | 0.535 2 | satt571 | I | 0.693 1 | 0.373 4 | 0.499 4 | 0.572 2 |
satt197 | B1 | 0.066 5 | 0.042 4 | 0.012 8 | 0.231 4 | satt431 | J | 0.930 3 | 0.502 0 | 0.394 9 | 0.440 3 |
satt168 | B2 | 0.725 1 | 0.379 8 | 0.479 8 | 0.558 2 | satt414 | K | 0.584 7 | 0.326 5 | 0.580 0 | 0.639 8 |
satt556 | B2 | 0.927 3 | 0.478 0 | 0.426 1 | 0.471 3 | satt242 | K | 0.682 3 | 0.400 3 | 0.428 1 | 0.516 8 |
satt194 | C1 | 0.747 1 | 0.442 2 | 0.427 3 | 0.491 4 | sct-099 | L | 0.871 6 | 0.473 8 | 0.413 9 | 0.466 3 |
Satt180 | C1 | 0.342 8 | 0.220 4 | 0.540 7 | 0.710 4 | satt373 | L | 0.601 4 | 0.346 7 | 0.486 9 | 0.584 1 |
satt307 | C2 | 0.634 9 | 0.339 7 | 0.484 0 | 0.587 6 | satt346 | M | 0.479 8 | 0.291 4 | 0.587 5 | 0.668 4 |
satt281 | C2 | 0.932 5 | 0.470 6 | 0.441 8 | 0.484 2 | satt590 | M | 0.651 4 | 0.363 6 | 0.487 7 | 0.572 9 |
satt386 | D2 | 0.677 0 | 0.394 4 | 0.498 3 | 0.558 2 | satt339 | N | 0.521 9 | 0.305 6 | 0.518 0 | 0.629 0 |
satt002 | D2 | 0.869 4 | 0.457 4 | 0.455 6 | 0.499 0 | satt530 | N | 0.800 0 | 0.401 8 | 0.473 1 | 0.540 7 |
satt268 | E | 0.774 0 | 0.427 8 | 0.439 6 | 0.506 8 | satt243 | O | 0.881 3 | 0.464 3 | 0.407 1 | 0.467 2 |
satt230 | E | 0.967 9 | 0.497 0 | 0.361 8 | 0.421 3 | satt487 | O | 0.795 0 | 0.422 0 | 0.459 0 | 0.521 0 |
satt185 | E | 0.682 4 | 0.394 9 | 0.489 3 | 0.553 4 | satt345 | O | 0.127 4 | 0.080 0 | 0.078 3 | 0.494 7 |
satt146 | F | 0.496 3 | 0.282 7 | 0.597 2 | 0.678 7 | satt216 | D1b+W | 0.649 9 | 0.359 5 | 0.525 3 | 0.593 7 |
satt334 | F | 0.184 7 | 0.107 8 | 0.719 3 | 0.869 7 | satt005 | D1b+W | 0.726 1 | 0.405 1 | 0.452 8 | 0.527 8 |
satt352 | G | 0.444 3 | 0.246 4 | 0.326 4 | 0.569 8 | satt267 | D1a+Q | 0.552 2 | 0.328 4 | 0.389 5 | 0.542 5 |
40个位点各参数的平均 Average genetic parameters for 40 loci | 0.658 2 | 0.362 1 | 0.445 9 | 0.544 2 |
Table 4 Estimates of genetic diversity for SSR loci in natural populations of wild soybean in Beijing
位点Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传 多样度Hs | 居群间遗传多样度DST | 基因分 化度GST | 位点 Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传多样度Hs | 居群间遗传 多样度DST | 基因分 化度GST |
---|---|---|---|---|---|---|---|---|---|---|---|
satt236 | A1 | 0.850 1 | 0.422 4 | 0.345 3 | 0.449 8 | satt309 | G | 0.466 7 | 0.282 3 | 0.569 7 | 0.668 7 |
satt300 | A1 | 0.746 2 | 0.414 5 | 0.434 8 | 0.512 0 | satt279 | H | 0.756 0 | 0.437 8 | 0.407 5 | 0.482 1 |
satt390 | A2 | 0.749 5 | 0.402 8 | 0.432 6 | 0.517 8 | satt434 | H | 0.759 6 | 0.403 3 | 0.432 5 | 0.517 5 |
satt429 | A2 | 0.767 3 | 0.390 7 | 0.443 5 | 0.531 6 | satt239 | I | 0.503 0 | 0.300 2 | 0.424 6 | 0.585 8 |
satt453 | B1 | 0.710 5 | 0.403 5 | 0.464 6 | 0.535 2 | satt571 | I | 0.693 1 | 0.373 4 | 0.499 4 | 0.572 2 |
satt197 | B1 | 0.066 5 | 0.042 4 | 0.012 8 | 0.231 4 | satt431 | J | 0.930 3 | 0.502 0 | 0.394 9 | 0.440 3 |
satt168 | B2 | 0.725 1 | 0.379 8 | 0.479 8 | 0.558 2 | satt414 | K | 0.584 7 | 0.326 5 | 0.580 0 | 0.639 8 |
satt556 | B2 | 0.927 3 | 0.478 0 | 0.426 1 | 0.471 3 | satt242 | K | 0.682 3 | 0.400 3 | 0.428 1 | 0.516 8 |
satt194 | C1 | 0.747 1 | 0.442 2 | 0.427 3 | 0.491 4 | sct-099 | L | 0.871 6 | 0.473 8 | 0.413 9 | 0.466 3 |
Satt180 | C1 | 0.342 8 | 0.220 4 | 0.540 7 | 0.710 4 | satt373 | L | 0.601 4 | 0.346 7 | 0.486 9 | 0.584 1 |
satt307 | C2 | 0.634 9 | 0.339 7 | 0.484 0 | 0.587 6 | satt346 | M | 0.479 8 | 0.291 4 | 0.587 5 | 0.668 4 |
satt281 | C2 | 0.932 5 | 0.470 6 | 0.441 8 | 0.484 2 | satt590 | M | 0.651 4 | 0.363 6 | 0.487 7 | 0.572 9 |
satt386 | D2 | 0.677 0 | 0.394 4 | 0.498 3 | 0.558 2 | satt339 | N | 0.521 9 | 0.305 6 | 0.518 0 | 0.629 0 |
satt002 | D2 | 0.869 4 | 0.457 4 | 0.455 6 | 0.499 0 | satt530 | N | 0.800 0 | 0.401 8 | 0.473 1 | 0.540 7 |
satt268 | E | 0.774 0 | 0.427 8 | 0.439 6 | 0.506 8 | satt243 | O | 0.881 3 | 0.464 3 | 0.407 1 | 0.467 2 |
satt230 | E | 0.967 9 | 0.497 0 | 0.361 8 | 0.421 3 | satt487 | O | 0.795 0 | 0.422 0 | 0.459 0 | 0.521 0 |
satt185 | E | 0.682 4 | 0.394 9 | 0.489 3 | 0.553 4 | satt345 | O | 0.127 4 | 0.080 0 | 0.078 3 | 0.494 7 |
satt146 | F | 0.496 3 | 0.282 7 | 0.597 2 | 0.678 7 | satt216 | D1b+W | 0.649 9 | 0.359 5 | 0.525 3 | 0.593 7 |
satt334 | F | 0.184 7 | 0.107 8 | 0.719 3 | 0.869 7 | satt005 | D1b+W | 0.726 1 | 0.405 1 | 0.452 8 | 0.527 8 |
satt352 | G | 0.444 3 | 0.246 4 | 0.326 4 | 0.569 8 | satt267 | D1a+Q | 0.552 2 | 0.328 4 | 0.389 5 | 0.542 5 |
40个位点各参数的平均 Average genetic parameters for 40 loci | 0.658 2 | 0.362 1 | 0.445 9 | 0.544 2 |
种群Population | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|---|
1 | **** | 0.210 5 | 0.392 6 | 0.193 2 | 0.222 6 | 0.214 9 | 0.218 7 | 0.180 8 | 0.194 3 | 0.192 6 |
2 | 1.558 4 | **** | 0.245 5 | 0.053 1 | 0.340 0 | 0.200 3 | 0.210 1 | 0.289 4 | 0.206 8 | 0.194 1 |
3 | 0.935 0 | 1.404 6 | **** | 0.266 0 | 0.363 4 | 0.214 9 | 0.211 2 | 0.212 4 | 0.296 1 | 0.276 8 |
4 | 1.643 9 | 2.935 0 | 1.324 2 | **** | 0.206 9 | 0.107 0 | 0.170 2 | 0.090 1 | 0.120 8 | 0.152 6 |
5 | 1.502 5 | 1.078 8 | 1.012 2 | 1.575 7 | **** | 0.356 6 | 0.265 2 | 0.331 9 | 0.238 2 | 0.241 4 |
6 | 1.537 6 | 1.607 8 | 1.537 4 | 2.235 0 | 1.031 0 | **** | 0.253 3 | 0.238 1 | 0.259 8 | 0.195 6 |
7 | 1.519 9 | 1.560 3 | 1.554 8 | 1.770 8 | 1.327 2 | 1.373 1 | **** | 0.296 2 | 0.244 6 | 0.176 7 |
8 | 1.710 4 | 1.240 1 | 1.549 1 | 2.406 4 | 1.103 1 | 1.434 9 | 1.216 6 | **** | 0.374 8 | 0.340 1 |
9 | 1.638 4 | 1.575 9 | 1.217 1 | 2.113 2 | 1.434 7 | 1.348 0 | 1.408 1 | 0.981 5 | **** | 0.457 5 |
10 | 1.646 9 | 1.639 4 | 1.284 5 | 1.879 8 | 1.421 1 | 1.631 7 | 1.733 3 | 1.078 5 | 0.781 9 | **** |
Table 5 Nei’s genetic identity and genetic distance among 10 natural populations of wild soybean in Beijing
种群Population | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|---|
1 | **** | 0.210 5 | 0.392 6 | 0.193 2 | 0.222 6 | 0.214 9 | 0.218 7 | 0.180 8 | 0.194 3 | 0.192 6 |
2 | 1.558 4 | **** | 0.245 5 | 0.053 1 | 0.340 0 | 0.200 3 | 0.210 1 | 0.289 4 | 0.206 8 | 0.194 1 |
3 | 0.935 0 | 1.404 6 | **** | 0.266 0 | 0.363 4 | 0.214 9 | 0.211 2 | 0.212 4 | 0.296 1 | 0.276 8 |
4 | 1.643 9 | 2.935 0 | 1.324 2 | **** | 0.206 9 | 0.107 0 | 0.170 2 | 0.090 1 | 0.120 8 | 0.152 6 |
5 | 1.502 5 | 1.078 8 | 1.012 2 | 1.575 7 | **** | 0.356 6 | 0.265 2 | 0.331 9 | 0.238 2 | 0.241 4 |
6 | 1.537 6 | 1.607 8 | 1.537 4 | 2.235 0 | 1.031 0 | **** | 0.253 3 | 0.238 1 | 0.259 8 | 0.195 6 |
7 | 1.519 9 | 1.560 3 | 1.554 8 | 1.770 8 | 1.327 2 | 1.373 1 | **** | 0.296 2 | 0.244 6 | 0.176 7 |
8 | 1.710 4 | 1.240 1 | 1.549 1 | 2.406 4 | 1.103 1 | 1.434 9 | 1.216 6 | **** | 0.374 8 | 0.340 1 |
9 | 1.638 4 | 1.575 9 | 1.217 1 | 2.113 2 | 1.434 7 | 1.348 0 | 1.408 1 | 0.981 5 | **** | 0.457 5 |
10 | 1.646 9 | 1.639 4 | 1.284 5 | 1.879 8 | 1.421 1 | 1.631 7 | 1.733 3 | 1.078 5 | 0.781 9 | **** |
[1] | Akkaya MS, Bhagwat AA, Cregan PB (1992). Length polymorphisms of simple sequence repeat DNA in soybean. Genetics, 132,1131-1139. |
[2] | Chen YW, Nelson RL (2004). Genetic variation and relationships among cultivated, wild, and semiwild soybean. Crop Science, 44,316-325. |
[3] | Choi IY, Kang JH, Song HS, Kim NS (1999). Genetic diversity measured by simple sequence repeat variations among the wild soybean ( Glycine soja) collected along riverside of five major rivers in Korea . Genes and Genetic Systems, 74,169-177. |
[4] | Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, VanToai TT, Lohnes DG, Chung J, Specht JE (1999). An integrated genetic linkage map of the soybean genome. Crop Science, 39,1464-1490. |
[5] | Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus, 12,13-15. |
[6] | Fang WG (方卫国), Wei YT (韦宇拓), Pei Y (裴炎) (2000). An effective silver staining protocol for DNA. Hereditas (遗传), 22,167-168. (in Chinese with English abstract) |
[7] | Fujita R, Ohara M, Okazaki K, Shimamoto Y (1997). The extent of natural cross-pollination in wild soybean (Glycine soja). Journal of Heredity, 88,124-128. |
[8] | Fu YL (府宇雷), Qian J (钱吉), Ma YH (马玉虹), Li J (李军), Zheng SZ (郑师章) (2002). Genetic different- tiation research on populations of wild soybeans in different scales. Acta Ecologica Sinica (生态学报), 22,176-184. (in Chinese with English abstract) |
[9] | Gottlieb LD (1981). Electrophoretic evidence and plant populations. Progress in Phytochemistry, 7,1-46. |
[10] | Guan RX (关荣霞), Liu XM (刘秀敏), Chang RZ (常汝镇), Ning HX (宁惠霞), Yuan CP (袁翠平), Liu ZX (刘章雄), Qiu LJ (丘丽娟) (2006). Genetic diversity analysis of wild soybean ( Glycine soja Sieb. & Zucc.) from in-situ conserved population in Xinbin County of Liaoning Province . Chinese High Technology Letters (高技术通讯), 16,67-72. (in Chinese with English abstract) |
[11] | Hai L (海林), Wang KJ (王克晶), Yang K (杨凯) (2002). Genetic diversity of semi-wild soybean using SSR markers. Acta Botanica Boreali-occidentalia Sinica (西北植物学报), 22,751-757. (in Chinese with English abstract) |
[12] | Hu ZA (胡志昂), Wang HX (王洪新) (1985). Genetic structure of natural populations of wild soybean ( Glycine soja) in Beijing region . Acta Botanica Sinica (植物学报), 27,599-604. (in Chinese with English abstract) |
[13] | Jin Y (金燕), Lu BR (卢宝荣) (2003). Sampling strategy for genetic diversity. Chinese Biodiversity (生物多样性), 11,155-161. (in Chinese with English abstract) |
[14] | Jin Y, Zhang WJ, Fu DX, Lu BR (2003). Sampling strategy within a wild soybean population based on its genetic variation detected by ISSR markers. Acta Botanica Sinica (植物学报), 45,995-1002. |
[15] | Kiang YT, Chiang YC, Kaizuma N (1992). Genetic diversity in natural populations of wild soybean in iwate prefecture, Japan. Journal of Heredity, 83,325-329. |
[16] | Li J (李军), Tao Y (陶芸), Zheng SZ (郑师章), Zhou JL (周纪纶) (1995). Isozymatic differentiation in local population of Glycine soja Sieb. & Zucc . Acta Botanica Sinica (植物学报), 37,669-676. (in Chinese with English abstract) |
[17] | Li XH (李向华), Tian ZG (田子罡), Li FS (李福山) (2003). Genetic analysis of newly collected wild soybean materials and conserved germplasm collected from the same places. Journal of Plant Genetic Resources (植物遗传资源学报), 4,345-349. (in Chinese with English abstract) |
[18] | Li XH (李向华), Wang KJ (王克晶), Li FS (李福山), Yan MF (严茂粉) (2005). Research progress of wild soybean ( Glycine soja) and suggestions for improving its effective utilization and protection . Soybean Science (大豆科学), 24,305-309. (in Chinese with English abstract) |
[19] | Li ZL, Nelson RL (2001). RAPD marker diversity among cultivated and wild soybean accessions from four Chinese provinces. Crop Science, 41,1337-1347. |
[20] | Loveless MD, Hamrick JL (1984). Ecological determinants of genetic structure in plant populations. Annual Review of Ecology and Systematics, 15,93-99. |
[21] | Maughan PJ, Saghai Maroof MA, Buss GR (1995). Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean. Genome, 38,715-723. |
[22] | Morgante M, Rafalski A, Biddle P, Tingey S, Olivieri AM (1994). Genetic mapping and variability of seven soybean simple sequence repeat loci. Genome, 37,763-769. |
[23] | Nei M (1972). Genetic distance between populations. American Naturalist, 106,283-292. |
[24] | Nei M (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America, 70,3321-3323. |
[25] | Nei M (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89,583-590. |
[26] | Nei M (1987). Molecular Evolutionary Genetics. Columbia University Press, New York. |
[27] | Pei YL (裴颜龙), Wang L (王岚), Ge S (葛颂), Wang LZ (王连铮) (1996). Studies on genetic diversity of Glycine soja-isozymevariation in four populations . Soybean Science (大豆科学), 15,302-308. (in Chinese with English abstract) |
[28] | Powell W, Morgante M, Doyle JJ, McNicol JW, Tingey SV, Rafalski AJ (1996). Genepool variation in genus Glycine subgenus Soja revealed by polymorphic nuclear and chloroplast microsatellites . Genetics, 144,793-803. |
[29] | Shannon CE, Weaver W (1963). The Mathematical Theory of Communication. Illinois University Press, Urbana. |
[30] | Sjögren P, Wyöni PI (1994). Conservation genetics and detection of rare alleles in finite populations. Conservation Biology, 8,267-270. |
[31] | Song Q, Choi IY, Heo NK, Kim NS (1998). Genotype fingerprinting, differentiation and association between morphological traits and SSR loci of soybean landraces. Plant Research, 1,81-91. |
[32] | Tozuka A, Fukushi H, Hirata T, Ohara M, Kanazawa A, Mikami T, Abe1 J, Shimamoto1 Y (1998). Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA. Theoretical and Applied Genetics, 96,170-176. |
[33] | Wang KJ (王克晶), Li FS (李福山) (2000). General situation of wild soybean ( G. soja) germplasm resources and its utilization of introgression into cultivated soybean in China . Review of China Agricultural Science and Technology (中国农业科技导报), 2(6),69-72. (in Chinese with English abstract) |
[34] | Wang KJ (王克晶), Li FS (李福山), Chao YS (曹永生), Zhou T (周涛) (2001). Quantitative character structures of the natural populations of wild soybean in Hebei Province. Acta Phytoecologica Sinica (植物生态学报), 25,351-358. (in Chinese with English abstract) |
[35] | Wang KJ (王克晶), Li XH (李向华), Zhang ZW (张志卫), Li FS (李福山), Chao YS (曹永生) (2005). Components and distribution of 100-seed weight in natural populations of wild soybean. Soybean Science (大豆科学), 24,243-248. (in Chinese with English abstract) |
[36] | Wang KJ, Takahata Y (2007). A preliminary comparative evaluation of genetic diversity between Chinese and Japanese wild soybean germplasm pools using SSR markers. Genetic Resources and Crop Evolution, 54,157-165. |
[37] |
Xu DH, Abe J, Gai JY, Shimamoto Y (2002). Diversity of chloroplast DNA SSRs in wild and cultivated soybeans: evidence for multiple origins of cultivated soybean. Theoretical and Applied Genetics, 105,645-653.
URL PMID |
[38] | Yu H, Kiang YT (1993). Genetic variation in South Korean natural populations of wild soybean (Glycine soja). Euphytica, 68,213-221. |
[39] | Zhao HK (赵洪锟), Wang YM (王玉民), Li QY (李启云), Zhang M (张明), Zhuang BC (庄炳昌) (2001). SSR analysis of wild soybean ( G. soja) and cultivated soybean from different latitude in China . Soybean Science (大豆科学), 20,172-176. (in Chinese with English abstract) |
[40] | Zhao RZ (赵茹), Cheng Z (程舟), Lu BR (卢宝荣) (2006). Estimate of genetic diversity and sampling strategy based on molecular markers in one single population of wild soybean. Chinese Science Bulletin (科学通报), 51,1042-1048. (in Chinese with English abstract) |
[41] | Zhou XF (周晓馥), Zhuang BC (庄炳昌), Wang YM (王玉民), Zhao HK (赵洪琨) (2002). Population differentiation of wild soybean based on the RAPD and SSR analysis. Chinese Journal of Eco-Agriculture (中国生态农业学报), 4,6-9. (in Chinese with English abstract) |
[42] | Zhu WY (朱维岳), Zhou TY (周桃英), Zhong M (钟明), Lu BR (卢宝荣) (2006). Sampling strategy for wild soybean (Glycine soja) populations based on their genetic diversity and fine-scale spatial genetic structure. Journal of Fudan University (Natural Science Edition) (复旦大学学报(自然科学版)), 45,321-327. (in Chinese with English abstract) |
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