Rhizospheric soil of seedlings of Elaeagnus mollis colonized by arbuscular mycorrhizal fungi

doi:10.3773/j.issn.1005-264x.2010.06.007

Abstract

Abstract:

Aim Arbuscular mycorrhizal (AM) fungi were inoculated into the seedlings of Elaeagnus mollis, an endangered plant in China. Our objectives were to study the impact of AM fungi inoculation on the soil micro-area in the rhizosphere of E. mollis seedlings using a greenhouse pot experiment.

Methods The experimental design included separate inoculation (Glomus mosseae = GM or Acaulospora delicata = AD) and mixed inoculation (GM + AD); control groups were not inoculated. We determined the infection rate of AM fungi, biomass, infection rate of mycorrhizae, number of microorganisms in the rhizosphere, soil pH, soil enzyme activity, N and P nutrients in the rhizosphere, etc.

Important findings AM fungi infected the three inoculated groups, among which the infection rate of GM + AD was the greatest, up to 90.5%. Compared with the control group, the biomass of inoculated groups improved markedly (p < 0.05) and the biomass of the GM + AD group was 2.2 times that of the CK group. AM mycorrhizae impacted microbial populations on the roots; the number of bacteria, actinomycetes and nitrogen-fixing bacteria on the root surface increased significantly (p < 0.05). The AM mycorrhizae made the pH in the rhizosphere decrease, and this was significantly negatively correlated with the infection rate (p < 0.05). The activity of soil phosphatase, urease and protease in the rhizosphere of inoculated groups increased. Moreover, the increase of their activity was significantly correlated with mycorrhizal infection rate (p < 0.01). The phenomenon that the N and P elements directly absorbed by plants enriched the rhizosphere soil of inoculated groups was significantly correlated with the mycorrhizal infection rate (p < 0.05). AM formation improved the micro-ecological environment of E. mollis seedlings and increased soil fertility in the rhizosphere.

Key words: arbuscular mycorrhizal fungi, Elaeagnus mollis, rhizospheric soil, soil enzyme activity, pH, soil nutrient

YUAN Li-Huan, YAN Gui-Qin. Rhizospheric soil of seedlings of Elaeagnus mollis colonized by arbuscular mycorrhizal fungi[J]. Chin J Plant Ecol, 2010, 34(6): 678-686.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2010.06.007

https://www.plant-ecology.com/EN/Y2010/V34/I6/678

Figures/Tables 6

References 37

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处理 Treatment	苗高 Height of seedlings (cm)	地径 Diameter at ground (cm)	生物量 Biomass				菌根侵染率 Colonization rate (%)
处理 Treatment	苗高 Height of seedlings (cm)	地径 Diameter at ground (cm)	根干重 Dry weight of roots (g)	茎干重 Dry weight of stems (g)	叶干重 Dry weight of leaves (g)	全株干重 Whole plant dry weight (g)	菌根侵染率 Colonization rate (%)
GM	14.85 ± 1.82^a	0.26 ± 0.04^b	1.08 ± 0.08^a	1.02 ± 0.09^a	1.04 ± 0.08^a	3.14 ± 0.75^b	72.5 ± 7.28^b
AD	14.73 ± 1.89^a	0.25 ± 0.02^b	0.72 ± 0.09^b	0.67 ± 0.07^b	0.70 ± 0.07^b	2.09 ± 0.84^c	42.4 ± 4.41^c
GM + AD	15.01 ± 1.54^a	0.29 ± 0.03^a	1.20 ± 0.08^a	1.11 ± 0.06^a	1.07 ± 0.09^a	3.38 ± 0.96^a	90.5 ± 9.25^a
CK	13.17 ± 1.85^b	0.18 ± 0.02^c	0.48 ± 0.07^c	0.58 ± 0.04^c	0.49 ± 0.05^c	1.55 ± 0.63^d	0

处理 Treatment	苗高 Height of seedlings (cm)	地径 Diameter at ground (cm)	生物量 Biomass				菌根侵染率 Colonization rate (%)
处理 Treatment	苗高 Height of seedlings (cm)	地径 Diameter at ground (cm)	根干重 Dry weight of roots (g)	茎干重 Dry weight of stems (g)	叶干重 Dry weight of leaves (g)	全株干重 Whole plant dry weight (g)	菌根侵染率 Colonization rate (%)
GM	14.85 ± 1.82^a	0.26 ± 0.04^b	1.08 ± 0.08^a	1.02 ± 0.09^a	1.04 ± 0.08^a	3.14 ± 0.75^b	72.5 ± 7.28^b
AD	14.73 ± 1.89^a	0.25 ± 0.02^b	0.72 ± 0.09^b	0.67 ± 0.07^b	0.70 ± 0.07^b	2.09 ± 0.84^c	42.4 ± 4.41^c
GM + AD	15.01 ± 1.54^a	0.29 ± 0.03^a	1.20 ± 0.08^a	1.11 ± 0.06^a	1.07 ± 0.09^a	3.38 ± 0.96^a	90.5 ± 9.25^a
CK	13.17 ± 1.85^b	0.18 ± 0.02^c	0.48 ± 0.07^c	0.58 ± 0.04^c	0.49 ± 0.05^c	1.55 ± 0.63^d	0

处理Treatment	磷酸酶 Phosphatase (Phenol mg·g^-1·d^-1)				蛋白酶 Protease (NH₂-N mg·g^-1·d^-1)	脲酶 Urease (NH₃-N mg·g^-1·d^-1)
处理Treatment	碱性磷酸酶 Alkaline phosphatase	中性磷酸酶 Neutral phosphatase	酸性磷酸酶 Acid phosphatase	磷酸酶总量 Total phosphatase	蛋白酶 Protease (NH₂-N mg·g^-1·d^-1)	脲酶 Urease (NH₃-N mg·g^-1·d^-1)
GM	2.018^a	1.487^b	0.445^b	3.950^a	0.814 5^ab	1.252^a
AD	1.998^ab	1.201^b	0.431^b	3.630^b	0.756 1^b	0.854^b
GM + AD	2.019^a	1.521^b	0.554^a	4.094^a	1.002 8^a	1.326^a
CK	1.667^b	1.141^b	0.235^c	3.043^b	0.605 8^b	0.523^c

处理Treatment	磷酸酶 Phosphatase (Phenol mg·g^-1·d^-1)				蛋白酶 Protease (NH₂-N mg·g^-1·d^-1)	脲酶 Urease (NH₃-N mg·g^-1·d^-1)
处理Treatment	碱性磷酸酶 Alkaline phosphatase	中性磷酸酶 Neutral phosphatase	酸性磷酸酶 Acid phosphatase	磷酸酶总量 Total phosphatase	蛋白酶 Protease (NH₂-N mg·g^-1·d^-1)	脲酶 Urease (NH₃-N mg·g^-1·d^-1)
GM	2.018^a	1.487^b	0.445^b	3.950^a	0.814 5^ab	1.252^a
AD	1.998^ab	1.201^b	0.431^b	3.630^b	0.756 1^b	0.854^b
GM + AD	2.019^a	1.521^b	0.554^a	4.094^a	1.002 8^a	1.326^a
CK	1.667^b	1.141^b	0.235^c	3.043^b	0.605 8^b	0.523^c