Chin J Plan Ecolo ›› 2017, Vol. 41 ›› Issue (7): 787-794.DOI: 10.17521/cjpe.2016.0322
• Method and Technology • Previous Articles Next Articles
Feng-Jiao SHEN, Qian-Qian REN, Qi DONG, Li ZHU, Jian-Fang ZHANG, Jing YANG, Ran ZHANG, Hong-Zhu LIANG, Jian-Cheng ZHAO, Shuo SHI*()
Received:
2016-10-17
Accepted:
2017-06-01
Online:
2017-07-10
Published:
2017-08-21
Contact:
Shuo SHI
About author:
KANG Jing-yao(1991-), E-mail:
Feng-Jiao SHEN, Qian-Qian REN, Qi DONG, Li ZHU, Jian-Fang ZHANG, Jing YANG, Ran ZHANG, Hong-Zhu LIANG, Jian-Cheng ZHAO, Shuo SHI. A new angiosperms molecular specimen treatment method for field use[J]. Chin J Plan Ecolo, 2017, 41(7): 787-794.
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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2016.0322
干燥方式 Drying method | 日本晚樱 Prunus serrulata var. lannesiana | 山麦冬 Liriope spicata | ||||
---|---|---|---|---|---|---|
OD260/280 | DNA (ng·μL-1) | PCR (ng·μL-1) | OD260/280 | DNA (ng·μL-1) | PCR (ng·μL-1) | |
150 | 1.83 ± 0.17a | 451.24 ± 150.91a | 13.94 ± 3.97c | 2.03 ± 0.04a | 211.43 ± 64.31a | 0.74 ± 2.30d |
80 | 1.80 ± 0.13a | 376.13 ± 121.04a | 34.48 ± 9.42b | 1.83 ± 0.16b | 80.64 ± 48.16c | 16.51 ± 17.49c |
40 | 1.74 ± 0.16ab | 470.44 ± 228.50a | 49.07 ± 4.83a | 1.90 ± 0.08ab | 241.41 ± 88.07a | 57.64 ± 6.51a |
Y | 1.73 ± 0.09ab | 291.64 ± 90.77b | 38.38 ± 4.75b | 1.93 ± 0.14ab | 160.01 ± 22.95b | 46.98 ± 8.22b |
S | 1.64 ± 0.10b | 432.59 ± 167.67a | 39.95 ± 5.82b | 1.90 ± 0.24ab | 252.33 ± 61.74a | 43.19 ± 8.72b |
Table 1 Comparisons of DNA purity, concentration and the concentration of PCR products in the specimens of Prunus serrulata var. lannesiana and Liriope spicata obtained with different drying methods (mean ± SD)
干燥方式 Drying method | 日本晚樱 Prunus serrulata var. lannesiana | 山麦冬 Liriope spicata | ||||
---|---|---|---|---|---|---|
OD260/280 | DNA (ng·μL-1) | PCR (ng·μL-1) | OD260/280 | DNA (ng·μL-1) | PCR (ng·μL-1) | |
150 | 1.83 ± 0.17a | 451.24 ± 150.91a | 13.94 ± 3.97c | 2.03 ± 0.04a | 211.43 ± 64.31a | 0.74 ± 2.30d |
80 | 1.80 ± 0.13a | 376.13 ± 121.04a | 34.48 ± 9.42b | 1.83 ± 0.16b | 80.64 ± 48.16c | 16.51 ± 17.49c |
40 | 1.74 ± 0.16ab | 470.44 ± 228.50a | 49.07 ± 4.83a | 1.90 ± 0.08ab | 241.41 ± 88.07a | 57.64 ± 6.51a |
Y | 1.73 ± 0.09ab | 291.64 ± 90.77b | 38.38 ± 4.75b | 1.93 ± 0.14ab | 160.01 ± 22.95b | 46.98 ± 8.22b |
S | 1.64 ± 0.10b | 432.59 ± 167.67a | 39.95 ± 5.82b | 1.90 ± 0.24ab | 252.33 ± 61.74a | 43.19 ± 8.72b |
Fig. 1 Genomic DNA in specimens of Prunus serrulata var. lannesiana (R) obtained with five different drying methods. 150, drying at 150 °C; 80, drying at 80 °C; 40, drying at 40 °C; Y, natural drying; S, silica gel drying; 2K, 2 kb plus DNA ladder.
Fig. 2 Genomic DNA in specimens of Liriope spicata (M) obtained with five different drying methods. 50, drying at 150 °C; 80, drying at 80 °C; 40, drying at 40 °C; Y, natural drying; S, silica gel drying; 2K, 2 kb plus DNA ladder.
Fig. 3 Agarose gel electrophoresis of PCR products in specimens of Prunus serrulata var. lannesiana (R) obtained with five different drying methods. 150, drying at 150 °C; 80, drying at 80 °C; 40, drying at 40 °C; Y, natural drying; S, silica gel drying; 2K, 2kb plus DNA ladder.
Fig. 4 Agarose gel electrophoresis of PCR products in specimens of Liriope spicata (M) obtained with five different drying methods. 150, drying at 150 °C; 80, drying at 80 °C; 40, drying at 40 °C; Y, natural drying; S, silica gel drying; 2K, 2 kb plus DNA ladder.
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