Chin J Plant Ecol ›› 2019, Vol. 43 ›› Issue (12): 1079-1090.doi: 10.17521/cjpe.2019.0055

• Research Articles • Previous Articles     Next Articles

Difference in fungal communities between in roots and in root-associated soil of nine orchids in Liaoning, China

JIANG Yu-Ling1,CHEN Xu-Hui1,MIAO Qing1,QU Bo1,2,*()   

  1. 1 College of Biological Technology, Shenyang Agricultural University, Shenyang 110161, China
    2 Key Laboratory of Biological Invasions and Global Changes, Shenyang 110161, China
  • Received:2019-03-12 Accepted:2019-11-18 Online:2020-01-03 Published:2019-12-20
  • Contact: QU Bo E-mail:syauqb@163.com
  • Supported by:
    Supported by the Natural Science Foundation of Liaoning Province(201602656)

Abstract:

Aims Orchid plants generally grow better when they are mycorrhizal since mycorrhizal fungi are likely to assist in orchid seeds’ germination. However, there is little quantitative work on it. Thus we hope to better understand this mechanism to benefit the orchid plants protection.
Methods We studied nine small population species of orchids grown in Liaoning Province, China. We analyzed the composition of orchid mycorrhizal fungi (OMF) and fungal communities in the roots, in the rhizosphere soil as well as bulk soil, by taking advantage of the next generation sequencing technology.
Important findings Our study showed that there was a significant difference in fungal communities among in the roots, the rhizosphere soil and the bulk soil, especially in the total operational taxonomic unit (OTU) number. Although the OTU number was far smaller in the roots than in the rhizosphere soil and bulk soil, the species and abundances of OMF were less relative to each other. FunGuild, an indicator to predict the functional fungi, indicated that Arbuscular Mycorrhizal fungi were abundence in the rhizosphere while were rare in the roots of orchids. In general, the fungal communities in the roots were not tightly correlated with that in the root-associated soil.

Key words: orchids, mycorrhizal fungi, soil, internal transcribed spacer (ITS), high-throughput sequencing

Table 1

Species identification and location of nine orchids in Liaoning, China"

植物种类
Species
株数
Number
采集地点
Location
生境
Biotope
生育期
Growth stage
无柱兰
Amitostigma gracile
1 辽宁大连庄河市塔子沟
Tazigou, Zhuanghe, Dalian, Liaoning
阔叶林下水边悬崖
Waterside cliff under a broad-leaved forest
营养期
Vegetative period
长苞头蕊兰
Cephalanthera
longibracteata
1 辽宁大连庄河市塔子沟
Tazigou, Zhuanghe, Dalian, Liaoning
针阔混交林
Mixed broadleaf-conifer forest
花期
Flowering phase
小斑叶兰
Goodyera repens
1 辽宁大连庄河市塔子沟
Tazigou, Zhuanghe, Dalian, Liaoning
阔叶林下水边悬崖
Waterside cliff under a broad-leaved forest
花期
Flowering phase
二叶舌唇兰
Platanthera chlorantha
4 辽宁凤城市、凌源市、本溪市与庄河市
Fengcheng, Lingyuan, Benxi and Zhuanghe, Liaoning
针阔混交林
Mixed broadleaf-conifer forest
营养期、花期
Vegetative period
& Flowering phase
蜻蜓兰
Tulotis fuscescens
1 辽宁丹东凤城市
Fengcheng, Dandong, Liaoning
针阔混交林
Mixed broadleaf-conifer forest
花期
Flowering phase
羊耳蒜
Liparis campylostalix
1 辽宁大连庄河市姑庵庙
Gu’anmiao, Zhuanghe, Dalian, Liaoning
针阔混交林
Mixed broadleaf-conifer forest
营养期
Vegetative period
珊瑚兰
Corallorhiza trifida
1 辽宁凌源与河北承德市交界处
Junction of Lingyuan, Liaoning and Chengde, Hebei
阔叶林
Broad-leaved forest
营养期
Vegetative period
绶草
Spiranthes sinensis
3 辽宁阜新市杜家店水库
Dujiadian Reservoir, Fuxin, Liaoning
湖泊边草甸
Meadow beside a lake
花期
Flowering phase
山兰
Oreorchis patens
2 辽宁本溪市本溪县沟门和老秃顶子
Goumen and Laotudingzi in Benxi County, Benxi, Liaoning
针阔混交林
Mixed broadleaf-conifer forest
花期
Flowering phase

Fig. 1

Principal Component Analysis (PCA) of fungal communities in roots and soils of nine orchids in Liaoning, China. A, root; B, rhizosphere soil; C, bulk soil."

Fig. 2

UPGMA clustering analysis of fungal communities in roots and soils of nine orchids in Liaoning, China based on Unifrac distance. Samples are clustered according to their similarity, and shorter branching length means more similar. b, root; c, rhizosphere soil; d, bulk soil. Uppercase letters and number in right column indicate sample number."

Fig. 3

Comparative analysis of differences in the fungal communities of roots and soils based on Unifrac distance. A, root; B, rhizosphere soil; C, bulk soil."

Fig. 4

Top 15 fungi in root, rhizosphere soil and bulk soil of nine orchids in Liaoning, China."

Fig. 5

Trophic mode of fungi in orchid’s root, rhizosphere soil and bulk soil of nine orchids in Liaoning, China."

Fig. 6

Guild of fungi in orchid’s root, rhizosphere soil and bulk soil of nine orchids in Liaoning Province, China."

Fig. 7

Comparison of mycorrhizal fungi Spiranthes sinensis’s root, rhizosphere soil and bulk soil in Liaoning."

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