Chin J Plan Ecolo ›› 2013, Vol. 37 ›› Issue (8): 750-757.doi: 10.3724/SP.J.1258.2013.00078

• Research Articles • Previous Articles     Next Articles

Microbial community diversity in the rhizosphere of wetland plants examined by phospholipid fatty acid and polymerase chain reaction denaturing gradient gel electrophoresis

WANG Ai-Li*   

  1. College of Chemistry and Chemical Engineering, Dezhou University, Dezhou, Shandong 253023, China
  • Received:2013-02-05 Revised:2013-06-18 Online:2013-08-07 Published:2013-08-01
  • Contact: WANG Ai-Li E-mail:sxz_li@126.com

Abstract:

Aims Our objective was to investigate the effect of plant growth pattern (single or mixed) of two emergent plants (i.e., Phragmites australis and Typha orientalis) on the microbial biomass and community structure of the rhizosphere in two adjacent shallow lakes in Tianjin, China.
Methods The microbial biomass and community structure were assayed by using phospholipid fatty acid (PLFA) and polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) techniques. Cluster analysis of the microbial PLFA profiles was performed using hierarchical clustering according to the between-groups linkage method using the software package SPSS 13.0 for Windows.
Important findings The PLFA results showed that microbial biomass in the plant rhizosphere was higher than those in the non-rhizosphere, except for the P. australis rhizosphere in Aiwan Lake. The microbial biomass was significantly higher in the T. orientalis rhizosphere sediments than that in the P. australis rhizosphere sediments in both lakes. The microbial biomass of the same plant’s rhizosphere was influenced by pattern of plant growth (single or mixed). As the growth condition (plant height) was similar in the two lakes, there was only minor difference in the microbial biomass of the T. orientalis rhizosphere. When the two plants grew together, the growth of P. australis was significantly inhibited, and significantly lower microbial biomass was assayed than that under the separated growing condition. The gram-positive bacteria (G+) were less than that of the gram-negative bacteria (G) in all samples. Moreover, the ratio of G+ to G in the plant rhizosphere was less than in the non-rhizosphere. Microbial community structure was found to be related to the plant species. Little difference in microbial community structure was observed in same plant rhizosphere sediments between the two lakes; however, apparent differences in the bacterial community were detected between the two different plant species.

[1]Amann R I, Ludwig W, Schleifer K H (1995). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. .Microbiology and Molecular Biology Reviews,59:143-169 [2]Dong X L, Reddy G B(2010).Soil bacterial communities in constructed wetlands treated with swine wastewater using PCR-DGGE technique..Bioresource Technology,101:1175-1182 [3]Fan S X, Li P J, Gong Z Q, Ren WX, He N (2008).Promotion of pyrene degradation in rhizosphere of alfalfa (Medicago sativa L.). .Chemosphere,71:1593-1598 [4]Hallberg K B, Johnson B D (2005). Microbiology of a wetland ecosystem constructed to remediate mine drainage from a heavy metal mine. .Science of the Total Environment,338:53-66 [5]Heinrich D, Hess D (1985). Chemotactic attraction of Azospirillum lipoferum by wheat roots and characterization of some attractants. .Canadian Journal of Microbiology,31:26-31 [6]Iwamoto T, Tani K, Nakamura K, Suzuki Y, Kitagawa M, Equchi M, Nasu M (2000). Monitoring impact of in suit biostimulation treatment on ground water bacterial community by DGGE. .FEMS Microbiology Ecology,32:129-141 [7]Jenkins M B, Lion L W(1993).Mobile bacteria and transport of poly-nuclear aromatic hydrocarbons in porous media..Applied and Environmental Microbiology,59:3306-3313 [8]Li M, Zhou Q H, Tao M, Wang Y, Jiang L J, Wu Z B (2010). Comparative study of microbial community structure in different filter media of constructed wetland..Journal of Environmental Sciences,22:127-133 [9]Ravit B, Ehrenfeld J G, H?ggblom M M, bartels M (2007). The effects of drainage and nitrogen enrichment on Phragmites australis, Spartina alterniflora, and their root-associated microbial communities. .Wetlands,27:915-927 [10]Ruiz-Rueda O, Hallin S, Baneras L (2009). Structure and function of denitrifying and nitrifying bacteria communities in relation to the plant species in a constructed wetland. .FEMS Microbiology Ecology,67:308-319 [11]Sirivedhim T, Gray K A (2006). Factors affecting denitrification rates in experimental wetlands: field and laboratory studies. .Ecological Engineering,26:167-181 [12]Xiang X M, Song C X, Li Y S, Sun X Y (2004). Microorganism features of Typha Latifolia and Phragmites Australis at rhizosphere. (in Chinese with English abstract) [项学敏, 宋春霞, 李彦生, 孙祥宇 (2004). 湿地植物芦苇和香蒲根际微生物特性研究. 环境保护科学, 30, 35-38.].Chinese Journal of Environmental Protection Science,30:35-38 [13]Xue D, Yao H Y, Ge D Y, Huang C Y (2008). Soil microbial community structure in diverse land use system: A comparative study using Biolog, DGGE, and PLFA analyses. .Pedosphere,18:653-663 [14]Zelles L (1997). Phospholipid fatty acids profiles in selected members of soil microbial communities. .Chemosphere,35:275 -294 [15]Zhang Q R, Zhou Q X, Ren L P, Zhu Y G, Sun S L (2006).Ecological effects of crude oil residues on the functional diversity of soil microorganisms in three weed rhizospheres..Journal of Environmental Science,18:1101-1106
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[2] . Factors Affecting in vitro Rooting of Camptotheca acuminata[J]. Chin Bull Bot, 2004, 21(06): 673 -681 .
[3] Jiang Yue-ling. Effect of the Pollution by Phenol in Water on the Growth of Rice Seedlings[J]. Chin Bull Bot, 1997, 14(02): 41 -44 .
[4] Ying Liu, Baozhang Chen,Jing Chen,Guang Xu . Applicability of Evapotranspiration Simulation Models for Forest Ecosystems in Qianyanzhou[J]. Chin Bull Bot, 2016, 51(2): 226 -234 .
[5] Yang Guang-xiao. Effects of EMS on Anther Culture of Rice in Vitro[J]. Chin Bull Bot, 1990, 7(02): 41 -45 .
[6] Hou Fu-jiang and Ben Gui-ying. Advances in the Study on Effects of Ultraviolet-B Radiation on Plant[J]. Chin Bull Bot, 1997, 14(04): 18 -23 .
[7] Fang Wei Peng Shao-lin He Dao-quan. Population Dynamics of Successional Process of Secondary Forest in Baiyunshan of Guangzhou[J]. Chin Bull Bot, 1995, 12(专辑2): 55 -62 .
[8] Lan Wei. Effects of Using the Cold-resister CR-4 for Rice Seedling Culture in Nanning Area in 1992[J]. Chin Bull Bot, 1994, 11(特辑): 67 -71 .
[9] Tang Wei Gui Yao-lin Guo Zhong-chen Wu Jiang-yun. Organogenests in Protoplast Culture of Panax Ginseng[J]. Chin Bull Bot, 1995, 12(02): 49 -50 .
[10] Jin Wei;Chen Ping-liang;Gu Ying and Zhen Shen-zhi. Effects of Plant Growth Regulators on Lateral Bud Proliferation of Vaccinium[J]. Chin Bull Bot, 1991, 8(02): 53 -54 .