Chin J Plan Ecolo ›› 2009, Vol. 33 ›› Issue (5): 966-973.doi: 10.3773/j.issn.1005-264x.2009.05.016

• Research Articles • Previous Articles     Next Articles


LIU Wei*; WANG Xi; GAN You-Min; HUANG Lin-Kai; XIE Wen-Gang; MIAO Jia-Min   

  1. Department of Grassland Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
  • Online:2009-09-30 Published:2009-09-30
  • Contact: LIU Wei

Abstract: Aims Our objective was to explore differences in genetic diversity of Kobresia pygmaea populations related to grazing intensity to help prevent grassland degradation and contribute to ecology, genetics and evolutionary ecology.
Methods We randomly selected tender leaves from 25 K. pygmaea individuals from four populations along a gradient of grazing intensity, used SRAP (Sequence-related amplified polymorphism) molecular markers, and examined the genetic diversity of the populations to determine the effect of grazing dis-turbance.
Important findings We used 20 primers and produced 448 clear bands, 376 (83.93%) of which were polymorphic. With increased grazing intensity, the percentage of polymorphic loci, Nei’s genetic diversity index and Shannon information index of the K. pygmaea populations decreased. Total genetic di-versity (Ht) of the four populations under different grazing intensities was 0.276 6, genetic diversity within the four populations (Hs) was 0.243 6, genetic differentiation coefficient among populations (Gst) was 0.119 4 and gene flow (Nm*) was 1.843 4. With increased grazing intensity, Gst increased and gene flow decreased. Therefore, grazing increased genetic the differentiation coefficient among populations, restrained gene transfer among populations and promoted population gene differentiation. The genetic distance of the four populations with different grazing intensities was small; however, with increased grazing intensity, genetic distance slowly increased and genetic consistency among populations decreased. The UPGMA dendrogram constructed by genetic distance arrayed the four populations in order of increasing grazing pressure.

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[1] Hu Shi-yi. Fertilization in Plants IV. Fertilization Barriers Inoompalibilty[J]. Chin Bull Bot, 1984, 2(23): 93 -99 .
[2] JIANG Gao-Ming. On the Restoration and Management of Degraded Ecosystems: with Special Reference of Protected Areas in the Restoration of Degraded Lands[J]. Chin Bull Bot, 2003, 20(03): 373 -382 .
[3] . [J]. Chin Bull Bot, 1994, 11(专辑): 65 .
[4] ZHANG Xiao-Ying;YANG Shi-Jie. Plasmodesmata and Intercellular Trafficking of Macromolecules[J]. Chin Bull Bot, 1999, 16(02): 150 -156 .
[5] Chen Zheng. Arabidopsis thaliana as a Model Species for Plant Molecular Biology Studies[J]. Chin Bull Bot, 1994, 11(01): 6 -11 .
[6] . [J]. Chin Bull Bot, 1996, 13(专辑): 13 -16 .
[7] LEI Xiao-Yong HUANG LeiTIAN Mei-ShengHU Xiao-SongDAI Yao-Ren. Isolation and Identification of AOX (Alternative Oxidase) in ‘Royal Gala’ Apple Fruits[J]. Chin Bull Bot, 2002, 19(06): 739 -742 .
[8] Chunpeng Yao;Na Li. Research Advances on Abscisic Acid Receptor[J]. Chin Bull Bot, 2006, 23(6): 718 -724 .
[9] Li Wang, Qinqin Wang, Youqun Wang. Cytochemical Localization of ATPase and Acid Phosphatase in Minor Veins of the Leaf of Vicia faba During Different Developmental Stages[J]. Chin Bull Bot, 2014, 49(1): 78 -86 .
[10] QIU Dong-Liang, LIU Xing-Hui, GUO Su-Zhi. Effects of Simulated Acid Rain Stress on Gas Exchange and Chlorophyll a Fluorescence Parameters in Leaves of Longan[J]. Chin J Plan Ecolo, 2002, 26(4): 441 -446 .