GENETIC AND CLONAL DIVERSITY OF GLOBBA RACEMOSA, A HERB WITH A MIXED REPRODUCTIVE MODE

doi:10.3773/j.issn.1005-264x.2008.04.003

Abstract

Abstract:

Aims Our objective was to understand the relative effects of reproductive mode, geographic distance and other potential factors on within- and among-population genetic and clonal diversity in Globba racemosa, a species that can reproduce sexually (via seeds) and asexually (via bulbils).

Methods We investigated reproduction variation in the seven natural G. racemosa populations in Yuannan Province, China by measuring the number of sexually produced fruits and asexually produced bulbils. Genetic data were collected using inter-simple sequence repeat (ISSR) markers. We evaluated the distribution of genetic variance and levels of population genetic diversity and assessed the relationship between genetic diversity and reproduction variation.

Important findings Globba racemosa has a relatively high genetic diversity at the species level (PPB=71.19%), and most genetic variances resided among populations (G_ST = 0.590 7). A high clonal diversity was found in this species (G/N = 0.88). No significant difference was detected between reproductive mode and genetic diversity or clonal diversity, which suggested that the mode of reproduction was not necessarily a determinant of population genetic diversity. There was a significant correlation between genetic and geographical distances (r = 0.68, p < 0.05), which indicated that genetic differentiation between populations was likely attributed to the effect of isolation by distance. Other factors (e.g., small sexual recruitment, somatic mutation and founder effect, etc.) could also play important roles in maintaining genetic and clonal diversity in G. racemosa.

Key words: genetic diversity, clonal diversity, Globba racemosa, ISSR, mantel test, sexual vs. clonal reproduction

ZHOU Hui-Ping, CHEN Jin, ZHANG Shou-Zhou. GENETIC AND CLONAL DIVERSITY OF GLOBBA RACEMOSA, A HERB WITH A MIXED REPRODUCTIVE MODE[J]. Chin J Plant Ecol, 2008, 32(4): 751-759.

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https://www.plant-ecology.com/EN/Y2008/V32/I4/751

Figures/Tables 6

References 59

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	Pop1	Pop2	Pop3	Pop4	Pop5	Pop6	Pop7
Latitude/longitude	21o53′ N/ 100o58′ E	22o53′ N/ 99o48′ E	24o04′ N/ 97o48′ E	24o05′ N/ 99o44′ E	24o25′ N/ 97o50′ E	24o35′ N/ 97o40′ E	24o56′ N/ 102o29′ E
Habitat size (100 m²)	5-10	5-10	10-20	5-10	10-20	10-20	10-20
Altitude (m)	1 350	1 700	1 136	1 873	1 456	1 352	2 100
Soil water supply	Rich	Rich	Rich	Poor	Very rich	Poor	Very poor
Degree of disturbance	Slight	Slight	Undisturbed	Intense	Undisturbed	Intense	Slight
No. of fruits per plant (Mean ± 1 SE, n = 30)	1.65 ± 0.37	6.53 ± 0.62	0	0.80 ± 0.228	19.10 ± 1.58	0	0.20 ± 0.12
No. of bulbils per plant (Mean ± 1 SE, n = 30)	20. 12 ± 0.88	24. 67 ± 1.88	30.00 ± 1.90	24.27 ± 1.79	28.63 ± 0.77	18.83 ± 1.28	24.43 ± 1.30
Ratio of fruits/bulbils (Mean ± 1 SE, n = 30)	0.11 ± 0.03	0.34 ± 0.06	0	0.04 ± 0.01	0.67 ± 0.05	0	0.01 ± 0.01

	Pop1	Pop2	Pop3	Pop4	Pop5	Pop6	Pop7
Latitude/longitude	21o53′ N/ 100o58′ E	22o53′ N/ 99o48′ E	24o04′ N/ 97o48′ E	24o05′ N/ 99o44′ E	24o25′ N/ 97o50′ E	24o35′ N/ 97o40′ E	24o56′ N/ 102o29′ E
Habitat size (100 m²)	5-10	5-10	10-20	5-10	10-20	10-20	10-20
Altitude (m)	1 350	1 700	1 136	1 873	1 456	1 352	2 100
Soil water supply	Rich	Rich	Rich	Poor	Very rich	Poor	Very poor
Degree of disturbance	Slight	Slight	Undisturbed	Intense	Undisturbed	Intense	Slight
No. of fruits per plant (Mean ± 1 SE, n = 30)	1.65 ± 0.37	6.53 ± 0.62	0	0.80 ± 0.228	19.10 ± 1.58	0	0.20 ± 0.12
No. of bulbils per plant (Mean ± 1 SE, n = 30)	20. 12 ± 0.88	24. 67 ± 1.88	30.00 ± 1.90	24.27 ± 1.79	28.63 ± 0.77	18.83 ± 1.28	24.43 ± 1.30
Ratio of fruits/bulbils (Mean ± 1 SE, n = 30)	0.11 ± 0.03	0.34 ± 0.06	0	0.04 ± 0.01	0.67 ± 0.05	0	0.01 ± 0.01