Chin J Plan Ecolo ›› 2011, Vol. 35 ›› Issue (10): 1083-1090.doi: 10.3724/SP.J.1258.2011.01083

• Research Articles • Previous Articles    

Genetic diversity of arbuscular mycorrhizal fungi in karst microhabitats of Guizhou Province, China

WEI Yuan1,2*, WANG Shi-Jie1**, LIU Xiu-Ming1, and HUANG Tian-Zhi1,2   

  1. 1Puding Karst Ecosystem Research Station, State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences,
    Guiyang 550002, China;

    2Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-05-10 Revised:2011-06-26 Online:2011-11-07 Published:2011-10-01
  • Contact: WANG Shi-Jie


Aims Our objective was to explore the genetic diversity of arbuscular mycorrhizal fungi (AMF) in karst microhabitat.
Methods We used nested polymerase chain reaction and denaturing gradient gel electrophoresis to study the genetic characteristics of AMF sampled from microhabitats of three different vegetation types in Maolan National Nature Reserve of Guizhou Province.
Important findings The AMF genetic diversities in all three microhabitat types were rich. The highest values of biodiversity index (4.06) and species abundance (68) were found in the shrubland soil surface, and the lowest values (3.16 and 29, respectively) were found in the secondary forest rocky crevice. The average values of biodiversity index and species abundance of the 9 microhabitats studied were 3.67 and 48, respectively. These were much higher than those in some other areas; the possible reasons were the complex karst ecological system structure and high plant species diversity. The AMF genetic diversity of different microhabitat types showed significant differences. The highest similarity index was only 0.45, and this means the spatial heterogeneity of different microhabitat types had significant influence on AMF genetic diversity. Glomus was the dominant genus through the karst area and should be a candidate for screening high ecological restoration strains for karst areas.

Cornejo P,Azcon-Aguilar C,Barea JM et al.2004.Temporal temperature gradient gelelectrophoresis(TTGE)as a tool for the characterization of arbuscular mycorrhizal fungi.FEMS Microbiology Letters, 241:265-270.

Fang H(房辉),P. N.Damodaran,CaoM(曹敏) (2006).arbuscular mycorrhizal status of Glomus plants in tropical secondary forest of Xishuangbanna, southwest china. Acta Ecologica Sinica(.生态学报), 26(12):4179-4185(in Chinese with English abstract)

Helgason T,Daniell TJ,Husband R et al.1998.Ploughing the word-wide-web?Nature,384:431

Kernaghan G.( 2005) Mycorrhizal diversity:Cause and effect? Pedobiologia, (49):511-520

Long LK(龙良鲲),Yang SZ(羊宋贞),Yao Q(姚青),Zhu HH(朱红惠). (2005). DNA extraction from arbuscular mycorrhizal fungi and analysis by Pcr-Denaturing Gradient Gel Electrophoresis. Mycosystema(菌物学报).24(4):564-569(in Chinese with English abstract)

Luo HF, Qi HY, Zhang HX. 2004.Assessment of the bacterial diversity in fenvalerate-treated soil. World Journal of Microbiology & Biotechnology,20: 509-515.

Li YB(李阳兵), Wang SJ(王世杰)(2006). An Analysis on the Frangibility of Karst Ecosystem. Tropical Geography(热带地理), 26(4): 303-307. (in Chinese with English abstract)

Liu F(刘方),Wang SJ(王世杰),Luo HB(罗海波),Liu YS(刘元生),Liu HY(刘鸿雁)(2008)。 Micro-habitats in katst forest ecosystem and variability of soils,Acta Pedologica sinica(土壤学报),45(6):1055-1062.(in Chinese with English abstract)

Li Y(李岩), Jiao H(焦惠), Xu LJ(徐丽娟),Zhao HH(赵洪海),Liu RJ(刘润进)(2010). Advances in the study of community structure and function of arbuscular mycorrhizl fungi. Acta Ecologica Sinica(生态学报), 30(4): 1089-1096. (in Chinese with English abstract)

Schwarzott D, Schüβler A.2001.A simple and reliable method for ssuRNA gene DNA extraction ,amplification and cloning from single AM fungal spore. Mycorrhiza,10:203-207

Simon L, Lalonde M, Bruns TD. 1992. Specific amplification of 18S fungi ribosomal genes from vesicular–arbuscular mycorrhizal fungi communities. Applied and Environmental Microbiology ,58:291-295

Smith S E, Read D J(1997). Mycorrhizal symbiosis [M]. London: Academic Press,1-20

Sykorová Z, Wiemken A, Redecker D. 2007.Co-occurring Gentiana verna and Gentiana acaulisand their neighboring plants in two Swis supper montane meadows harbor distinct arbuscular mycorrhizal fungi communities. Applied Environmental Microbiology, 73(17): 5426-5434.

Tu YL(屠玉麟)(1989).Preliminary study on karst forests in guizhou, Carsologica Sinica(中国岩溶), 8(4):282-290(in Chinese with English abstract)

Wang SJ(王世杰),Lu HM(卢红梅),Zhou YC(周运超),Xie LP(谢丽萍),Xiao DA(肖德安)(2007)。Spatial Variability of soil organic carbon and representative soil sampling method in maolan karst virgin forest. Acta pedoloqica sinica (土壤学报),44(3):475-483. (in Chinese with English abstract)

Wang L(王立), Jia WQ(贾文奇), Ma F(马放), Li SY(李世阳),Zhang SJ(张淑娟)(2010). Perspective of mycorrhizal technology application for environmental remediation. Ecology and Environmental Sciences(生态环境学报), 19(2): 487-493. (in Chinese with English abstract)

Yang RY(杨如意), Chen X(陈欣) , Tang JJ(唐建军),Chen J(陈静), Jiang QQ(蒋琦清), SJ HU(2005) Methods employed in species diversity determination of arbuscular mycorrhizal fungi(amf) colonized in terrestrial ecosystem, Bulletin of Science and Technology,(科技通报), 21(6):668-673(in Chinese with English abstract)

Zhou Z X(周政贤)(1987). Scientific Investigation Report of Maolan Karst Forest. Guiyang: Guizhou People’s Press, 1~23。(In Chinese)

Zhang Y (张英),Guo LD(郭良栋),Liu RJ(刘润进)( 2003).Diversity and ecology of arbuscular mycorrhizal fungi in dujiangyan .Acta phytoecologica sinica(植物生态学报), 27(4):537-544(in Chinese with English abstract)

Zhou YY(周游游),Li SS(黎树式),Huang TF(黄天放)(2003). Features of barst forest ecosystem in China and its conservation in utilisation-A case study on Maolan, Mulun, Nonggang typical karst forests in Southwest China. Journal of Guangxi Teachers Education University(NaturalScience Edition)(广西师范学院学报(自然科学版)), 16(3): 67-72.(in Chinese with English abstract)

Zhang MQ(张美庆),Wang YS(王幼珊),Xing LJ(邢礼军)( 1998).The ecologgical distribution of AM fungi community in south and east coast of china. Mycosystema(菌物系统), 17(3):274-277(in Chinese with English abstract)

Zhu SQ(朱守谦)(2003). Ecological Research on Karst Forest (Ⅲ) (In Chinese) . Guiyang: Guizhou Science and Technology Press. 52-62

Zhang MQ(张美庆),Wang YS(王幼珊),Xing LJ(邢礼军)(1999).The relationship between the distribution of AM fungi and environmental factors, Mycosystema(.菌物系统), 18(1):25-29(in Chinese with English abstract)
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[1] Yang Ying-gen;Zhang Li-jun and Li yu. Studies on the Postharvest Physiology properties of Peach Fruits[J]. Chin Bull Bot, 1995, 12(04): 47 -49 .
[2] Zhou Shi-gong. Applications of Lanthanum in Botanical Research[J]. Chin Bull Bot, 1992, 9(02): 26 -29 .
[3] . [J]. Chin Bull Bot, 1996, 13(专辑): 105 .
[4] 杜维广 王彬如 谭克辉 郝迺斌. An Approach to the Breeding of Soybean with High Photosynthetic Efficiency[J]. Chin Bull Bot, 1984, 2(23): 7 -11 .
[5] ZHAO Yun-Yun ZHOU Xiao-Mei YANG Cai. Production of Hybrid F1 Between Avena magna and Avena nuda and It''s Identification[J]. Chin Bull Bot, 2003, 20(03): 302 -306 .
[6] . Professor Jiayang Li, a Plant Molecular Genetist[J]. Chin Bull Bot, 2003, 20(03): 370 -372 .
[7] . [J]. Chin Bull Bot, 1996, 13(专辑): 100 -101 .
[8] Qiong Jiang, Youning Wang, Lixiang Wang, Zhengxi Sun, Xia Li. Validation of Reference Genes for Quantitative RT-PCR Analysis in Soybean Root Tissue under Salt Stress[J]. Chin Bull Bot, 2015, 50(6): 754 -764 .
[9] MA Ke-Ming. Advances of the Study on Species Abundance Pattern[J]. Chin J Plan Ecolo, 2003, 27(3): 412 -426 .