基于Lake模型的叶绿素荧光参数在甘蔗苗期抗旱性研究中的应用

doi:10.17521/cjpe.2015.0039

摘要/Abstract

摘要：

为从能量平衡及分配的角度研究干旱胁迫下甘蔗(Saccharum officinarum)苗期光系统的运转状况, 进而为丰富不同甘蔗品种的抗旱性评价指标及实现对季节性干旱胁迫的快速诊断提供理论依据, 该研究通过对基于Lake模型的叶绿素荧光参数在不同入射光强下变化的动态分析, 研究光合电子传递链中能量平衡状态对不同水分梯度(40%、25%、10%、8%)的响应。结果表明: 两个供试品种(耐旱品种‘ROC22’和非耐旱品种‘ROC16’)的最大光能利用效率(F_v/F_m)、相对电子传递速率(rETR)、光系统II(PSII)量子效率(Φ_II)和光化学猝灭(q_L)均随着干旱胁迫程度的增加而下降, 可调节性能量耗散(Φ_NPQ)和非调节性能量耗散(Φ_NO)则随着干旱胁迫程度的增加而上升。除Φ_NO之外的叶绿素荧光参数的变化幅度均随着光合有效辐射(PAR)的增加而增大。在干旱胁迫的前中期, 相对于‘ROC22’, ‘ROC16’的PSII反应中心能够维持较高的开放程度; 但‘ROC22’调节能量耗散的能力和对干旱胁迫的敏感程度均高于‘ROC16’, 说明较强的光保护能力是‘ROC22’的抗旱性高于‘ROC16’的主要原因之一。对干旱胁迫敏感且在不同PAR下较为稳定的Φ_NO可作为甘蔗苗期抗旱性的快速诊断和评价指标。rETR对递增的PAR的响应表现为随着干旱胁迫程度的增加而提前出现峰值或下降趋势, 但是不同水分梯度下的rETR在PAR较低时并无显著差异, 表明干旱胁迫下光抑制现象的提早出现是造成光系统损伤的首要因素, 高光强对干旱胁迫信号起放大作用。

关键词: 叶绿素荧光, 干旱胁迫, Lake模型, 甘蔗, 抗旱性评价

Abstract: Aims

Spring drought greatly limits the sugarcane (Saccharum officinarum) seedling growth in the southwest China. A major objective of this study is to investigate the change of the energy allocation in photosystem II (PSII) resulted from the stomatal and non-stomatal limit of photosynthesis under the drought stress condition. The study results can be used for drought resistant breeding and rapid drought stress diagnosis.

Methods

Four levels (40%, 25%, 10% and 8%) of soil volumetric water content (VWC) have been chosen from natural drought treatments. The chlorophyll fluorescence parameters based on Lake-model were measured and analyzed with various levels of photosynthetically active radiation (PAR).

Important findings

Results of the study in the enhanced drought stress for two tested cultivars (‘ROC22’ and ‘ROC16’) showed 1) decreased numbers in the maximum quantum use efficiency (F_v/F_m), the relative electron transport rate (rETR), the quantum efficiency of PSII (Φ_II) and the photochemical quenching (q_L) and 2) increased numbers in the down-regulated energy dissipation (Φ_NPQ) and the non-light induced energy dissipation (Φ_NO). There was no significant difference between the parameter q_L of ‘ROC16’ and ‘ROC22’, except for the 8% level where the q_L showed a sharp decline. The results indicated that the PSII of ‘ROC16’ remained a relatively high open fraction during the mild drought stress, but suffered severe damage in serious drought stress. The rising range of Φ_NPQ for ‘ROC22’ were higher and more sensitive to drought stress than that of ‘ROC16’, which revealed a strong drought resistance resulted from strong photo-protective mechanism in‘ROC22’. The Φ_NO was more sensitive to drought stress than F_v/F_m especially for non drought resistant variety, and remained high stability under different PAR. Therefore, Φ_NO could be properly used as an indicator in drought stress diagnosis and resistance evaluation. Photo inhibition could be the initial factor leading to PSII damage and its signal had been amplified under high PAR related to photo inhibition since the appeared peak or decline of rETR related to increased PAR and its no significance at each water level and low PAR during drought stress.

Key words: chlorophyll fluorescence, drought stress, Lake-model, sugarcane, drought resistance evaluation

安东升, 曹娟, 黄小华, 周娟, 窦美安. 基于Lake模型的叶绿素荧光参数在甘蔗苗期抗旱性研究中的应用. 植物生态学报, 2015, 39(4): 398-406. DOI: 10.17521/cjpe.2015.0039

AN Dong-Sheng,CAO Juan,HUANG Xiao-Hua,ZHOU Juan,DOU Mei-An. Application of Lake-model based indices from chlorophyll fluorescence on sugarcane seedling drought resistance study. Chinese Journal of Plant Ecology, 2015, 39(4): 398-406. DOI: 10.17521/cjpe.2015.0039

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2015.0039

https://www.plant-ecology.com/CN/Y2015/V39/I4/398

图/表 4

表1 断水后不同品种甘蔗土壤体积含水量(VWC)随干旱天数的变化

Table 1 Soil volumetric water content (VWC) of the two cultivars of Saccharum officinarum after drought treatment

处理天数 Days after treatment	品种 Cultivars
	‘ROC22’		‘ROC16’
	VWC (%)	标准偏差 Standard deviation (%)	VWC (%)	标准偏差 Standard deviation (%)
1	74.9	3.6	74.4	3.6
2	64.4	3.9	59.2	4.6
3	52.4	3.6	40.1	4.0
4	40.0	2.6	24.7	3.3
5	25.9	3.5	10.9	1.4
6	17.7	2.1	8.6	1.0
7	10.2	1.7	5.9	1.4
8	7.6	1.5	3.0	0.0

图1 不同光强下光系统II活性对干旱胁迫的响应(平均值±标准偏差)。rETR, 相对电子传递速率; ΦII, 光系统II量子效率; PAR, 光合有效辐射; VWC, 体积含水量。

Fig. 1 Effects of drought stress on photosystem II activities under different light intensity (mean ± SD). rETR, relative electron transport rate; ΦII, quantum efficiency of photosystem II; PAR, photosynthetically active radiation; VWC, volumetric water content.

图2 不同光强下叶绿素荧光猝灭对干旱胁迫的响应(平均值±标准偏差)。qL, 基于Lake模型的光化学猝灭; ΦNPQ, 可调节性能量耗散; ΦNO, 非调节性能量耗散; PAR, 光合有效辐射; VWC, 体积含水量。

Fig. 2 Effects of drought stress on chlorophyll fluorescence quenching under different light intensity (mean ± SD). qL, photochemical quenching based on Lake-model; ΦNPQ, down-regulated energy dissipation; ΦNPQ, non-light induced energy dissipation; PAR, photo-synthetically active radiation; VWC, volumetric water content.

图3 最大光能利用效率(Fv/Fm)和非调节性能量耗散(ΦNO)对干旱胁迫的响应(平均值±标准偏差); VWC, 体积含水量。

Fig. 3 Effects of drought stress on the maximum quantum use efficiency (Fv/Fm) and the non-light induced energy dissipation (ΦNO) (mean ± SD); VWC, volumetric water content.

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