植物生态学报 ›› 2020, Vol. 44 ›› Issue (8): 885-894.DOI: 10.17521/cjpe.2019.0358

• 研究论文 • 上一篇    

秦岭锐齿栎林土壤酶活性与化学计量比变化特征及其影响因素

解梦怡1,2, 冯秀秀1,2, 马寰菲1,2, 胡汗1,2, 王洁莹1,2, 郭垚鑫3, 任成杰4, 王俊1,2, 赵发珠1,2,*()   

  1. 1陕西省地表系统与环境承载力重点实验室, 西安 710127
    2西北大学城市与环境学院, 西安 710127
    3西北大学生命科学学院, 西安 710127
    4西北农林科技大学农学院, 陕西杨凌 712100
  • 收稿日期:2019-12-23 接受日期:2020-04-23 出版日期:2020-08-20 发布日期:2020-07-03
  • 通讯作者: 赵发珠
  • 作者简介:* zhaofazhu@nwu.edu.cn
  • 基金资助:
    国家自然科学基金(41601578);中国博士后特别资助(2018T111089)

Characteristics of soil enzyme activities and stoichiometry and its influencing factors in Quercus aliena var. acuteserrata forests in the Qinling Mountains

XIE Meng-Yi1,2, FENG Xiu-Xiu1,2, MA Huan-Fei1,2, HU Han1,2, WANG Jie-Ying1,2, GUO Yao-Xin3, REN Cheng-Jie4, WANG Jun1,2, ZHAO Fa-Zhu1,2,*()   

  1. 1Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity, Xi?an 710127, China
    2College of Urban and Environment Science, Northwest University, Xi?an 710127, China
    3College of Life Sciences, Northwest University, Xi?an 710127, China
    4College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Received:2019-12-23 Accepted:2020-04-23 Online:2020-08-20 Published:2020-07-03
  • Contact: ZHAO Fa-Zhu
  • Supported by:
    National Natural Science Foundation of China(41601578);China Postdoctoral Science Foundation(2018T111089)

摘要:

研究微尺度海拔梯度土壤酶活性与化学计量学比值的动态变化及驱动因素对于探讨生态系统养分循环过程具有重要意义。该研究以秦岭太白山6个海拔(分别为1 308、1 403、1 503、1 603、1 694和1 803 m)的锐齿栎(Quercus aliena var. acuteserrata)林作为研究对象, 测定锐齿栎叶片、凋落物、细根和土壤的碳(C)、氮(N)、磷(P)含量以及碱性磷酸酶(AKP)、β-1,4-葡萄糖苷酶(βG)、纤维二糖水解酶(CBH)、木糖苷酶(βX)与β-N-乙酰氨基葡萄糖苷酶(NAG)的活性, 探究不同海拔植物、土壤、酶含量如何变化及驱动土壤酶活性变化的主要因子。结果表明: 5种土壤酶活性在海拔梯度上表现出不同的变化趋势, CBH和βG活性随海拔升高整体呈先增后减趋势, βX与之相反; NAG与AKP活性在1 408-1 694 m呈下降趋势, 在1 803 m处有所升高; 土壤总体酶活性随海拔上升整体表现为降低趋势。相关性分析表明, 土壤酶活性及其化学计量比不同程度上受到植物和土壤C、N、P资源及土壤水热条件等的调控, 其中与土壤有机碳含量的相关性较高, 土壤有机碳含量可被认为是锐齿栎林中影响土壤酶活性变化的主要因子。总之, 土壤酶活性及化学计量比在微尺度海拔梯度上具有差异性, 且受到植物和土壤C、N、P资源的综合影响。

关键词: 生态化学计量比, 土壤酶活性, 养分循环, 海拔梯度, 锐齿栎林

Abstract:

Aims The dynamics and driving factors of soil enzyme activities and stoichiometry in the micro-scale elevation gradient is of great significance in the study of nutrient cycling processes.
Methods In the present study, the Quercus aliena var. acuteserrata forest belts at the elevation of 1 308, 1 403, 1 503, 1 603, 1 694 and 1 803 m in Taibai Mountain were sampled to determine the contents of carbon (C), nitrogen (N), and phosphorus (P) in leaves, litters, roots and soils, and the activities of alkaline phosphatase (AKP), β-1,4-glucosidase (βG), cellobiohydrolase (CBH), β-1,4-xylosidase (βX) and β-1,4-N-acetylglucosaminidase (NAG).
Important findings Our results showed that altitude had a great impact on the activities of five soil enzymes. CBH and βG increased first and then decreased with the altitude, while βX showed the opposite trend. The NAG and AKP activity showed a downward trend from 1 408 to 1 694 m and increased with elevation since 1 803 m. The total enzyme activity index exhibited a decreasing trend with altitudes increases. The correlation analysis results indicated that soil enzyme activities and their stoichiometry were controlled by plant, soil C, N, P resources, and soil water and heat conditions. Among these factors, the content of soil organic carbon had high correlation with these parameters and was the main factor affecting the change of soil enzyme activities in the Quercus aliena var. acuteserrata forest. In short, the soil enzyme activities and stoichiometry were different along the micro-scale elevation gradient, affected by the C, N, and P resources of plant and soil.

Key words: ecological stoichiometry, soil enzyme activity, nutrient cycle, elevation gradient, Quercus aliena var. acuteserrata forest