植物生态学报 ›› 2021, Vol. 45 ›› Issue (5): 528-538.DOI: 10.17521/cjpe.2020.0139

• 研究论文 • 上一篇    下一篇

青藏高原不同高寒草地类型土壤酶活性及其影响因子

汪子微1,2,3, 万松泽3, 蒋洪毛1,2, 胡扬1,2, 马书琴4, 陈有超5, 鲁旭阳1,*()   

  1. 1中国科学院、水利部成都山地灾害与环境研究所, 成都 610041
    2中国科学院大学, 北京 100049
    3江西农业大学林学院, 南昌 330045
    4河南师范大学旅游学院, 河南新乡 453007
    5中国科学院武汉植物园, 武汉 430074
  • 收稿日期:2020-05-11 接受日期:2020-07-17 出版日期:2021-05-20 发布日期:2020-08-10
  • 通讯作者: 鲁旭阳
  • 作者简介:*鲁旭阳:ORCID: 0000-0001-6653-6009(xylu@imde.ac.cn)
  • 基金资助:
    国家自然科学基金(41671262);国家自然科学基金(41877338)

Soil enzyme activities and their influencing factors among different alpine grasslands on the Qingzang Plateau

WANG Zi-Wei1,2,3, WAN Song-Ze3, JIANG Hong-Mao1,2, HU Yang1,2, MA Shu-Qin4, CHEN You-Chao5, LU Xu-Yang1,*()   

  1. 1Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China
    2University of Chinese Academy of Science, Beijing 100049, China
    3School of Forestry, Jiangxi Agricultural University, Nanchang 330045, China
    4College of Tourism, Henan Normal University, Xinxiang, Henan 453007, China
    5Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, China
  • Received:2020-05-11 Accepted:2020-07-17 Online:2021-05-20 Published:2020-08-10
  • Contact: LU Xu-Yang
  • Supported by:
    the National Natural Science Foundation of China(41671262);the National Natural Science Foundation of China(41877338)

摘要:

土壤酶活性作为生态系统养分循环的关键因素, 是反映土壤质量和生态系统功能的重要指标, 但是关于高寒草地生态系统中不同草地类型间酶活性的差异研究还很少。因此, 该研究在藏北高寒草地选择高寒草甸、高寒草原、高寒草甸草原、高寒荒漠草原和高寒荒漠5种草地类型进行野外原位调查和采样, 测定了涉及碳(C)、氮(N)和磷(P)循环的14种酶的活性, 并建立了高寒草地酶活性与土壤微生物和土壤理化性质等环境因子的关系。结果表明: C循环酶(蔗糖酶、纤维素酶、β-葡萄糖苷酶、多酚氧化酶和过氧化物酶)和P循环酶(碱性磷酸酶)在不同高寒草地类型间活性差异明显, N循环酶中仅芳香氨基酶和亚硝酸盐还原酶两种酶在不同高寒草地类型间活性差异明显。同时, C、N和P循环酶之间存在一定的相关关系, 其中, 蔗糖酶和碱性磷酸酶、纤维素酶和α-乙酰氨基葡萄糖苷酶活性显著正相关, 多酚氧化酶与亚硝酸还原酶和β-乙酰氨基葡萄糖苷酶活性显著负相关。在测定的19个环境指标中, 土壤有机质(SOM)含量、革兰氏阴性菌数量、土壤N和P含量计量比、革兰氏阳性菌数量、细菌数量、放线菌数量、全氮含量、真菌数量是影响土壤酶活性的关键因子, 且SOM含量的影响最大(解释量为11.9%)。综上所述, 不同高寒草地类型间C循环酶、P循环酶和两种N循环酶(芳香氨基酶和亚硝酸还原酶)活性差异显著, SOM含量、微生物数量和N含量等是影响高寒草地生态系统土壤酶活性的关键因子。

关键词: 高寒草甸, 高寒草原, 土壤酶活性, 土壤微生物, 环境因子

Abstract:

Aims As a key factor of nutrient cycling in ecosystems, soil enzyme activity is an important indicator of soil quality and ecosystem function. However, there have been very few studies on the differences of soil enzyme activities among different types of alpine grassland ecosystems. Thus, the aims of this study were to compare the differences of soil enzyme activities among five different types of alpine grassland and to reveal their influencing environmental factors on the Qingzang Plateau.
Methods Totally, 21 samples of five alpine grassland types, including alpine meadow, alpine steppe, alpine meadow steppe, alpine desert steppe and alpine desert on northern Qingzang Plateau, were selected for field in-situ investigation and sampling. The activities of 14 enzymes involved in the cycling of carbon (C), nitrogen (N) and phosphorus (P) were determined, and the relationships between enzymatic activities and environmental factors in alpine grassland were established.
Important findings The activities of C-acquisition (invertase, cellulase, β-1,4-glucosidse, polyphenol oxidase and peroxidase), P-acquisition (alkaline phosphatase) enzymes and two N-acquisition (arylamidase and nitrite reductase) were significantly different among different alpine grassland types. Moreover, correlations were found among C-acquisition, N-acquisition and P-acquisition enzymes. A significant positive correlation was found between invertase and alkaline phosphatase, and between cellulase and N-acetyl-α-D-glucosaminidase. A significant negative correlation was found between polyphenol oxidase and nitrite reductase, N-acetyl-β-D- glucosaminidase. Soil organic matter (SOM) content, gram-negative bacteria content, ratio of nitrogen to phosphorus, gram-positive bacteria content, bacteria content, actinomycetes content, total nitrogen content and fungi content were the key factors influencing soil enzyme activity among the 19 environmental indicators, and SOM content had the greatest impact (explained 11.9%). The results demonstrated that the activities of C-acquisition, P-acquisition and two N-acquisition (arylamidase and nitrite reductase) enzymes were significantly different among different types of alpine grassland, and soil enzyme activities were mainly controlled by SOM content, microbes and N elements in alpine grassland ecosystems.

Key words: alpine meadow, alpine steppe, soil enzyme activity, soil microbes, environmental factor