植物生态学报 ›› 2010, Vol. 34 ›› Issue (9): 1075-1083.DOI: 10.3773/j.issn.1005-264x.2010.09.008

• 研究论文 • 上一篇    下一篇

克隆整合有助于狗牙根抵御水淹

张想英1,2, 樊大勇1, 谢宗强1,*(), 熊高明1, 李兆佳1,2   

  1. 1中国科学院植物研究所植被与环境变化国家重点试验室, 北京 100093
    2中国科学院研究生院, 北京 100049
  • 收稿日期:2010-05-24 接受日期:2010-06-04 出版日期:2010-05-24 发布日期:2010-10-08
  • 通讯作者: 谢宗强
  • 作者简介:* E-mail: xie@ibcas.ac.cn

Clonal integration enhances performance of Cynodon dactylon subjected to submergence

ZHANG Xiang-Ying1,2, FAN Da-Yong1, XIE Zong-Qiang1,*(), XIONG Gao-Ming1, LI Zhao-Jia1,2   

  1. 1State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-05-24 Accepted:2010-06-04 Online:2010-05-24 Published:2010-10-08
  • Contact: XIE Zong-Qiang

摘要:

尽管国内外开展了大量的克隆整合对克隆植物抵御逆境能力影响的研究, 但整合对植物抵御水淹能力的影响研究仍比较缺乏。该文从克隆整合的角度探讨多年生草本植物狗牙根(Cynodon dactylon)对水淹胁迫的响应。试验模拟了先端分株(相对年幼的分株)分别处于0、5和15 cm三种水淹胁迫环境, 并在每个水淹梯度下实施先端分株与基端分株(相对年长的分株)之间匍匐茎连接或切断处理, 调查水淹一个月后基端分株和先端分株以及整个克隆片段在形态和生理上的表现。研究发现: 切断匍匐茎连接显著降低了狗牙根先端分株的生长, 表现在生物量下降、匍匐茎长度减短和分株数减少等方面; 水淹显著抑制了先端分株的生长, 但对基端分株的生长并未造成显著影响; 在5 cm水淹处理下, 匍匐茎保持连接时, 先端分株和整个克隆片段的生长显著增加; 连接或切断处理在不同水淹梯度下对匍匐茎平均节间长没有显著影响, 对先端分株或基端分株在光化学转化效率上也未表现显著性差异。结果表明: 克隆整合效应促进了狗牙根在水淹胁迫下分株的生长, 并有助于整个克隆片段抵御水淹胁迫。

关键词: 生物量, 荧光参数, 分株, 匍匐茎切断

Abstract:

Aims Despite extensive studies on effects of clonal integration in the past three decades, little is known about the effects of clonal integration on the performance of waterlogged clonal plants. Our objective was to test the hypothesis that clonal integration could improve performance of Cynodon dactylon, a stoloniferous clonal herb commonly found in riparian areas of reservoirs, under waterlogging stress.

Methods Relatively young apical ramets of C. dactylon clonal fragments were submerged in water at depths of 0 (control), 5 and 15 cm, and their connections to the relatively old basal ramets of the fragments under normal conditions were either severed (preventing clonal integration) or not (allowing integration). Performances of apical ramets, basal ramets and whole fragments were investigated after one month of treatment. Meanwhile, chlorophyll fluorescence parameters (Fv/Fm, maximum quantum yield of PSII; Yield, effective quantum yield of PSII) were measured.

Important findings Severing the stolon connection significantly decreased the growth of apical ramets under submergence in terms of biomass, total stolon length and number of ramets. Submergence also significantly decreased the growth of apical ramets, but had little effects on basal ramets. Performance of both basal ramets and clonal fragments was enhanced if the basal ramets were connected with the apical ramets subjected to 5 cm depth of submergence. However, stolon severing treatment had little effects on chlorophyll fluorescence parameters of both apical ramets and basal ramets. Results indicate that clonal integration increased performance of waterlogged ramets and the growth of non-waterlogged connected ramets. Clonal integration could help C. dactylon resist submergence stress.

Key words: biomass, fluorescence parameters, ramet, stolon severing