活性氧在UV-B诱导的玉米幼苗叶片乙烯产生中的作用

doi:10.17521/cjpe.2007.0120

摘要/Abstract

摘要：

研究了活性氧在UV-B(280~320 nm)诱导的玉米(Zea mays)幼苗叶片乙烯合成中的作用。结果表明,UV-B促进了玉米幼苗活性氧和乙烯的产生;乙烯合成抑制剂氨氧乙烯基甘氨酸(AVG)和氨氧乙酸(AOA)能明显减弱UV-B对玉米幼苗乙烯产生的诱导作用,但对活性氧(ROS)的产生没有明显影响;ROS的清除剂不但能抑制UV-B诱导的ROS的产生,而且还可以抑制UV-B诱导的乙烯的产生,但这种抑制作用可以被外源$\text{O}_{2}^{{\bar{.}}}$的供体所逆转。这说明,乙烯的积累不能作为UV-B胁迫下ROS的诱导的因素,相反,ROS的积累则导致了乙烯的积累;因此,ROS可能参与了UV-B胁迫诱导的乙烯的产生。质膜NADPH氧化酶的抑制剂二苯碘鎓(DPI)和H₂O₂的特异性清除剂过氧化氢酶(CAT)对UV-B胁迫诱导的乙烯积累几乎没有影响,这说明H₂O₂可能与UV-B诱导的玉米幼苗叶片乙烯的产生无关, 在UV-B诱导的玉米幼苗叶片乙烯的生物合成过程中$\text{O}_{2}^{{\bar{.}}}$起着很重要的作用,相关的$\text{O}_{2}^{{\bar{.}}}$不是由NADPH氧化酶催化产生的。

关键词: 活性氧, UV-B辐射, 乙烯, 玉米

Abstract:

Aims Ethylene accumulation and oxidative stress are two common responses of plants to environmental stresses; however, little is known about their relationships. Our objective was to investigate the role of reactive oxygen species (ROS) in ethylene synthesis induced by UV-B radiation (280-320 nm) in leaves of maize (Zea mays).

Methods Lamps were suspended above and perpendicular to the plastic trays and filtered with 0.13-mm thick cellulose diacetate (transmission down to 290 nm) for UV-B irradiance. The desired UV-B irradiation was obtained by changing the distance between the lamps and the plastic trays. The levels of UV-B irradiation were 4.8 kJ·m^-2·d^-1.

Important findings UV-B radiation led to the generation of ROS and ethylene. The accumulation of ethylene induced by UV-B was not only inhibited by the scavengers of ROS, but also by aminoxyacetic acid (AOA) and 2-aminoethoxyvinlglycine (AVG), which are specific inhibitors of ethylene synthesis. The inhibition effect of ROS scavengers on UV-B-induced ethylene production was reversed by $\text{O}_{2}^{{\bar{.}}}$ donors. Results indicated that the increase in ethylene production may not be the cause of the increase in ROS production under UV-B stress. In contrast, the increase of ROS led to the accumulation of ethylene. Results suggested that ROS are involved in UV-B stress-induced ethylene accumulation. The accumulation of UV-B-induced ethylene was not affected by DPI, an inhibitor of NADPH oxidase and CAT, a specific scavenger of H₂O₂. Results suggested that the effect of H₂O₂ on UV-B-induced ethylene production in leaves of maize seedlings can be excluded, $\text{O}_{2}^{{\bar{.}}}$ plays an important role in UV-B-induced ethylene synthesis in leaves of maize seedlings and $\text{O}_{2}^{{\bar{.}}}$ serves as a potential mediator of ethylene production that the plant can sense the UV-B stress. The related ROS are not from plasma membrane NADPH oxidase. The source of ROS contributing to ethylene accumulation under UV-B stress is unknown.

Key words: reactive oxygen species (ROS), ultraviolet-B radiation (UV-B), ethylene production, maize

王弋博, 冯虎元, 曲颖, 程佳强, $\boxed{\hbox{王勋陵}}$, 安黎哲. 活性氧在UV-B诱导的玉米幼苗叶片乙烯产生中的作用. 植物生态学报, 2007, 31(5): 946-951. DOI: 10.17521/cjpe.2007.0120

WANG Yi-Bo, FENG Hu-Yuan, QU Ying, CHENG Jia-Qiang, $\boxed{\hbox{WANG Xun-Ling}}$ , AN Li-Zhe. EFFECTS OF REACTIVE OXYGEN SPECIES ON UV-B-INDUCED ETHYLENE PRODUCTION IN LEAVES OF MAIZE SEEDLINGS. Chinese Journal of Plant Ecology, 2007, 31(5): 946-951. DOI: 10.17521/cjpe.2007.0120

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2007.0120

https://www.plant-ecology.com/CN/Y2007/V31/I5/946

图/表 4

图1 UV-B对玉米幼苗叶片$\text{O}_{2}^{{\bar{.}}}$ (a)、H2O2(b)及乙烯(c)产生速率的影响结果表示为3次独立实验的3次重复的平均值±标准差

Fig.1 Effects of UV-B radiation on the rate of $\text{O}_{2}^{{\bar{.}}}$ (a) 、H2O2(b) and ethylene (c)production in leaves of maize seedlings Bars represent standard deviations of three independent experiments with three replicates; Values are means of three triplicate±SE

图2 AOA和AVG对UV-B诱导的玉米幼苗叶片$\text{O}_{2}^{{\bar{.}}}$ (a)、H2O2(b)及乙烯(c)合成的影响图注见图1

Fig.2 Effects of AOA and AVG on UV-B induced $\text{O}_{2}^{{\bar{.}}}$ (a)、H2O2(b) and ethylene(c) accumulation in leaves of maize seedlings Note see Fig. 1

图3 处理5天后外源活性氧清除剂对UV-B诱导的玉米幼苗$\text{O}_{2}^{{\bar{.}}}$ (a)、H2O2(b)及乙烯(c)合成的影响图注见图1

Fig.3 Effects of exogenous ROS scavengers on UV-induced $\text{O}_{2}^{{\bar{.}}}$ (a)、H2O2(b) and ethylene(c) accumulation in leaves of maize seedlings Note see Fig. 1

图4 处理5天后外源超氧阴离子产生剂对UV-B胁迫下玉米幼苗乙烯产生的影响\ 图注见图1

Fig.4 Effects of exogenous $\text{O}_{2}^{{\bar{.}}}$ donor on UV-induced ethylene accumulation in leaves of maize seedlings after treatments for 5 days Note see Fig. 1

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