植物生态学报 ›› 2007, Vol. 31 ›› Issue (5): 946-951.DOI: 10.17521/cjpe.2007.0120

• 论文 • 上一篇    下一篇

活性氧在UV-B诱导的玉米幼苗叶片乙烯产生中的作用

王弋博1,2, 冯虎元2, 曲颖2, 程佳强2, $\boxed{\hbox{王勋陵}}$2, 安黎哲2,3,*()   

  1. 1 天水师范学院生物系, 甘肃天水 741000
    2 兰州大学生命科学学院, 兰州 730000
    3 中国科学院寒区旱区环境与工程研究所,兰州 730000
  • 收稿日期:2005-12-15 接受日期:2007-01-11 出版日期:2007-12-15 发布日期:2007-09-30
  • 通讯作者: 安黎哲
  • 作者简介:* E-mail: anlizhe@yahoo.com
  • 基金资助:
    国家自然科学基金资助项目(30170083);国家自然科学基金资助项目(30170186)

EFFECTS OF REACTIVE OXYGEN SPECIES ON UV-B-INDUCED ETHYLENE PRODUCTION IN LEAVES OF MAIZE SEEDLINGS

WANG Yi-Bo1,2, FENG Hu-Yuan2, QU Ying2, CHENG Jia-Qiang2, $\boxed{\hbox{WANG Xun-Ling}}$ 2, AN Li-Zhe2,3,*()   

  1. 1Department of Biology, Tianshui Normal College, Tianshui, Gansu 741000, China
    2School of Life Sciences, Lanzhou University, Lanzhou 730000, China
    3Cold and Arid Region Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2005-12-15 Accepted:2007-01-11 Online:2007-12-15 Published:2007-09-30
  • Contact: AN Li-Zhe

摘要:

研究了活性氧在UV-B(280~320 nm)诱导的玉米(Zea mays)幼苗叶片乙烯合成中的作用。结果表明,UV-B促进了玉米幼苗活性氧和乙烯的产生;乙烯合成抑制剂氨氧乙烯基甘氨酸(AVG)和氨氧乙酸(AOA)能明显减弱UV-B对玉米幼苗乙烯产生的诱导作用,但对活性氧(ROS)的产生没有明显影响;ROS的清除剂不但能抑制UV-B诱导的ROS的产生,而且还可以抑制UV-B诱导的乙烯的产生,但这种抑制作用可以被外源$\text{O}_{2}^{{\bar{.}}}$的供体所逆转。这说明,乙烯的积累不能作为UV-B胁迫下ROS的诱导的因素,相反,ROS的积累则导致了乙烯的积累;因此,ROS可能参与了UV-B胁迫诱导的乙烯的产生。质膜NADPH氧化酶的抑制剂二苯碘鎓(DPI)和H2O2的特异性清除剂过氧化氢酶(CAT)对UV-B胁迫诱导的乙烯积累几乎没有影响,这说明H2O2可能与UV-B诱导的玉米幼苗叶片乙烯的产生无关, 在UV-B诱导的玉米幼苗叶片乙烯的生物合成过程中$\text{O}_{2}^{{\bar{.}}}$起着很重要的作用,相关的$\text{O}_{2}^{{\bar{.}}}$不是由NADPH氧化酶催化产生的。

关键词: 活性氧, UV-B辐射, 乙烯, 玉米

Abstract:

Aims Ethylene accumulation and oxidative stress are two common responses of plants to environmental stresses; however, little is known about their relationships. Our objective was to investigate the role of reactive oxygen species (ROS) in ethylene synthesis induced by UV-B radiation (280-320 nm) in leaves of maize (Zea mays).

Methods Lamps were suspended above and perpendicular to the plastic trays and filtered with 0.13-mm thick cellulose diacetate (transmission down to 290 nm) for UV-B irradiance. The desired UV-B irradiation was obtained by changing the distance between the lamps and the plastic trays. The levels of UV-B irradiation were 4.8 kJ·m-2·d-1.

Important findings UV-B radiation led to the generation of ROS and ethylene. The accumulation of ethylene induced by UV-B was not only inhibited by the scavengers of ROS, but also by aminoxyacetic acid (AOA) and 2-aminoethoxyvinlglycine (AVG), which are specific inhibitors of ethylene synthesis. The inhibition effect of ROS scavengers on UV-B-induced ethylene production was reversed by $\text{O}_{2}^{{\bar{.}}}$ donors. Results indicated that the increase in ethylene production may not be the cause of the increase in ROS production under UV-B stress. In contrast, the increase of ROS led to the accumulation of ethylene. Results suggested that ROS are involved in UV-B stress-induced ethylene accumulation. The accumulation of UV-B-induced ethylene was not affected by DPI, an inhibitor of NADPH oxidase and CAT, a specific scavenger of H2O2. Results suggested that the effect of H2O2 on UV-B-induced ethylene production in leaves of maize seedlings can be excluded, $\text{O}_{2}^{{\bar{.}}}$ plays an important role in UV-B-induced ethylene synthesis in leaves of maize seedlings and $\text{O}_{2}^{{\bar{.}}}$ serves as a potential mediator of ethylene production that the plant can sense the UV-B stress. The related ROS are not from plasma membrane NADPH oxidase. The source of ROS contributing to ethylene accumulation under UV-B stress is unknown.

Key words: reactive oxygen species (ROS), ultraviolet-B radiation (UV-B), ethylene production, maize